Aqueous extract from Orthosiphon stamineus leaves prevents bladder and kidney infection in mice
Extracts from the leaves of Orthosiphon stamineus are used in phytotherapy for treatment of uncomplicated urinary tract infections. Evaluation of an aqueous extract against infection with uropathogenic Escherichia coli in vivo; investigation of underlying microbiological mechanisms. In vivo studies...
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| Veröffentlicht in: | Phytomedicine (Stuttgart) 2017-05, Vol.28, p.1-9 |
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| creator | Sarshar, S. Brandt, S. Asadi Karam, M.R. Habibi, M. Bouzari, S. Lechtenberg, M. Dobrindt, U. Qin, X. Goycoolea, F.M. Hensel, A. |
| description | Extracts from the leaves of Orthosiphon stamineus are used in phytotherapy for treatment of uncomplicated urinary tract infections.
Evaluation of an aqueous extract against infection with uropathogenic Escherichia coli in vivo; investigation of underlying microbiological mechanisms.
In vivo studies in mice and in vitro investigations on cytotoxicity, antiadhesive potential, influence on bacterial gene expression and quorum sensing.
Extract OWE was prepared by hot water extraction. For in vivo studies BALB/c mice were used in an UPEC infection model. The effect of OWE on bacterial load in bladder/kidney tissue was monitored in pre- and posttreatment. Cytotoxicity of OWE against different UPEC strains, T24 bladder/A498 kidney cells, gene expression analysis, monitoring of phenotypic motility and quorum sensing was investigated by standard methods of microbiology.
OWE was quantified (UHPLC) according to the content of rosmarinic acid, cichoric acid, caffeic acid. Three- and 5-day treatment of animals with OWE (750mg/kg) after transurethral infection with UPEC CFT073 reduced the bacterial load in bladder and kidney, similar to norfloxacin. Four- and 7-day pretreatment of mice prior to the infection with UPEC NU14 reduced bacterial bladder colonization. In vitro investigations indicated that OWE (≤2mg/ml) has no cytotoxic or proliferation-inhibiting activity against different UPEC strains as well as against T24 bladder and A498 kidney cells. OWE exerts a dose dependent antiadhesive activity against UPEC strains NU14 and UTI89. OWE reduced gene expression of fimH, but evoked increase of the expression of motility/fitness gene fliC. Increase of bacterial motility on gene level was confirmed by a changed bacterial phenotype by an increased bacterial motility in soft agar assay. OWE inhibited in a concentration-dependent manner bacterial quorum sensing.
OWE is assessed as a strong antiadhesive plant extract for which the traditional use in phytotherapy for UTI might be justified.
[Display omitted] |
| doi_str_mv | 10.1016/j.phymed.2017.02.009 |
| format | Article |
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Evaluation of an aqueous extract against infection with uropathogenic Escherichia coli in vivo; investigation of underlying microbiological mechanisms.
In vivo studies in mice and in vitro investigations on cytotoxicity, antiadhesive potential, influence on bacterial gene expression and quorum sensing.
Extract OWE was prepared by hot water extraction. For in vivo studies BALB/c mice were used in an UPEC infection model. The effect of OWE on bacterial load in bladder/kidney tissue was monitored in pre- and posttreatment. Cytotoxicity of OWE against different UPEC strains, T24 bladder/A498 kidney cells, gene expression analysis, monitoring of phenotypic motility and quorum sensing was investigated by standard methods of microbiology.
OWE was quantified (UHPLC) according to the content of rosmarinic acid, cichoric acid, caffeic acid. Three- and 5-day treatment of animals with OWE (750mg/kg) after transurethral infection with UPEC CFT073 reduced the bacterial load in bladder and kidney, similar to norfloxacin. Four- and 7-day pretreatment of mice prior to the infection with UPEC NU14 reduced bacterial bladder colonization. In vitro investigations indicated that OWE (≤2mg/ml) has no cytotoxic or proliferation-inhibiting activity against different UPEC strains as well as against T24 bladder and A498 kidney cells. OWE exerts a dose dependent antiadhesive activity against UPEC strains NU14 and UTI89. OWE reduced gene expression of fimH, but evoked increase of the expression of motility/fitness gene fliC. Increase of bacterial motility on gene level was confirmed by a changed bacterial phenotype by an increased bacterial motility in soft agar assay. OWE inhibited in a concentration-dependent manner bacterial quorum sensing.
OWE is assessed as a strong antiadhesive plant extract for which the traditional use in phytotherapy for UTI might be justified.
[Display omitted]</description><identifier>ISSN: 0944-7113</identifier><identifier>EISSN: 1618-095X</identifier><identifier>DOI: 10.1016/j.phymed.2017.02.009</identifier><identifier>PMID: 28478807</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Adhesion ; Animals ; Anti-Bacterial Agents - chemistry ; Anti-Bacterial Agents - pharmacology ; Antiadhesion ; Disease Models, Animal ; Epithelial Cells - drug effects ; Escherichia coli Infections - drug therapy ; Escherichia coli Infections - prevention & control ; Female ; Humans ; Kidney Diseases - microbiology ; Kidney Diseases - pathology ; Kidney Diseases - prevention & control ; Mice ; Mice, Inbred BALB C ; Orthosiphon - chemistry ; Orthosiphon stamineus ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Plant Leaves - chemistry ; Quorum sensing ; UPEC ; Urinary Bladder Diseases - microbiology ; Urinary Bladder Diseases - prevention & control ; Urinary Tract Infections - microbiology ; Uropathogenic E. coli ; Uropathogenic Escherichia coli - drug effects ; Uropathogenic Escherichia coli - pathogenicity</subject><ispartof>Phytomedicine (Stuttgart), 2017-05, Vol.28, p.1-9</ispartof><rights>2017 Elsevier GmbH</rights><rights>Copyright © 2017 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-8656d4fea95f8184f28f4ab1d611ff3acb886bc3336dc1f0df543e894312ec543</citedby><cites>FETCH-LOGICAL-c408t-8656d4fea95f8184f28f4ab1d611ff3acb886bc3336dc1f0df543e894312ec543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.phymed.2017.02.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28478807$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sarshar, S.</creatorcontrib><creatorcontrib>Brandt, S.</creatorcontrib><creatorcontrib>Asadi Karam, M.R.</creatorcontrib><creatorcontrib>Habibi, M.</creatorcontrib><creatorcontrib>Bouzari, S.</creatorcontrib><creatorcontrib>Lechtenberg, M.</creatorcontrib><creatorcontrib>Dobrindt, U.</creatorcontrib><creatorcontrib>Qin, X.</creatorcontrib><creatorcontrib>Goycoolea, F.M.</creatorcontrib><creatorcontrib>Hensel, A.</creatorcontrib><title>Aqueous extract from Orthosiphon stamineus leaves prevents bladder and kidney infection in mice</title><title>Phytomedicine (Stuttgart)</title><addtitle>Phytomedicine</addtitle><description>Extracts from the leaves of Orthosiphon stamineus are used in phytotherapy for treatment of uncomplicated urinary tract infections.
Evaluation of an aqueous extract against infection with uropathogenic Escherichia coli in vivo; investigation of underlying microbiological mechanisms.
In vivo studies in mice and in vitro investigations on cytotoxicity, antiadhesive potential, influence on bacterial gene expression and quorum sensing.
Extract OWE was prepared by hot water extraction. For in vivo studies BALB/c mice were used in an UPEC infection model. The effect of OWE on bacterial load in bladder/kidney tissue was monitored in pre- and posttreatment. Cytotoxicity of OWE against different UPEC strains, T24 bladder/A498 kidney cells, gene expression analysis, monitoring of phenotypic motility and quorum sensing was investigated by standard methods of microbiology.
OWE was quantified (UHPLC) according to the content of rosmarinic acid, cichoric acid, caffeic acid. Three- and 5-day treatment of animals with OWE (750mg/kg) after transurethral infection with UPEC CFT073 reduced the bacterial load in bladder and kidney, similar to norfloxacin. Four- and 7-day pretreatment of mice prior to the infection with UPEC NU14 reduced bacterial bladder colonization. In vitro investigations indicated that OWE (≤2mg/ml) has no cytotoxic or proliferation-inhibiting activity against different UPEC strains as well as against T24 bladder and A498 kidney cells. OWE exerts a dose dependent antiadhesive activity against UPEC strains NU14 and UTI89. OWE reduced gene expression of fimH, but evoked increase of the expression of motility/fitness gene fliC. Increase of bacterial motility on gene level was confirmed by a changed bacterial phenotype by an increased bacterial motility in soft agar assay. OWE inhibited in a concentration-dependent manner bacterial quorum sensing.
OWE is assessed as a strong antiadhesive plant extract for which the traditional use in phytotherapy for UTI might be justified.
[Display omitted]</description><subject>Adhesion</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - chemistry</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antiadhesion</subject><subject>Disease Models, Animal</subject><subject>Epithelial Cells - drug effects</subject><subject>Escherichia coli Infections - drug therapy</subject><subject>Escherichia coli Infections - prevention & control</subject><subject>Female</subject><subject>Humans</subject><subject>Kidney Diseases - microbiology</subject><subject>Kidney Diseases - pathology</subject><subject>Kidney Diseases - prevention & control</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Orthosiphon - chemistry</subject><subject>Orthosiphon stamineus</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Leaves - chemistry</subject><subject>Quorum sensing</subject><subject>UPEC</subject><subject>Urinary Bladder Diseases - microbiology</subject><subject>Urinary Bladder Diseases - prevention & control</subject><subject>Urinary Tract Infections - microbiology</subject><subject>Uropathogenic E. coli</subject><subject>Uropathogenic Escherichia coli - drug effects</subject><subject>Uropathogenic Escherichia coli - pathogenicity</subject><issn>0944-7113</issn><issn>1618-095X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMoun78A5EcvbRm2mybXgQRv0DwouAtpMmEzdqmNeku7r83y7pXTxnI887wPoRcAsuBQXWzzMfFpkeTFwzqnBU5Y80BmUEFImPN_POQzFjDeVYDlCfkNMYlY8Cbmh2Tk0LwWghWz4i8-17hsIoUf6ag9ERtGHr6FqbFEN24GDyNk-qdx4R0qNYY6RhwjX6KtO2UMRio8oZ-OeNxQ523qCeXYs7T3mk8J0dWdREv_t4z8vH48H7_nL2-Pb3c371mmjMxZaKaV4ZbVM3cChDcFsJy1YKpAKwtlW6FqFpdlmVlNFhm7JyXKBpeQoE6zWfkerd3DENqFCfZu6ix65Tf1pMgmooD57xJKN-hOgwxBrRyDK5XYSOBya1auZQ7tXKrVrJCJrUpdvV3YdVu__ahvcsE3O4ATD3XDoOM2qHXaFxIUqQZ3P8XfgFY4o4d</recordid><startdate>20170515</startdate><enddate>20170515</enddate><creator>Sarshar, S.</creator><creator>Brandt, S.</creator><creator>Asadi Karam, M.R.</creator><creator>Habibi, M.</creator><creator>Bouzari, S.</creator><creator>Lechtenberg, M.</creator><creator>Dobrindt, U.</creator><creator>Qin, X.</creator><creator>Goycoolea, F.M.</creator><creator>Hensel, A.</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20170515</creationdate><title>Aqueous extract from Orthosiphon stamineus leaves prevents bladder and kidney infection in mice</title><author>Sarshar, S. ; Brandt, S. ; Asadi Karam, M.R. ; Habibi, M. ; Bouzari, S. ; Lechtenberg, M. ; Dobrindt, U. ; Qin, X. ; Goycoolea, F.M. ; Hensel, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-8656d4fea95f8184f28f4ab1d611ff3acb886bc3336dc1f0df543e894312ec543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adhesion</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - chemistry</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antiadhesion</topic><topic>Disease Models, Animal</topic><topic>Epithelial Cells - drug effects</topic><topic>Escherichia coli Infections - drug therapy</topic><topic>Escherichia coli Infections - prevention & control</topic><topic>Female</topic><topic>Humans</topic><topic>Kidney Diseases - microbiology</topic><topic>Kidney Diseases - pathology</topic><topic>Kidney Diseases - prevention & control</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Orthosiphon - chemistry</topic><topic>Orthosiphon stamineus</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Leaves - chemistry</topic><topic>Quorum sensing</topic><topic>UPEC</topic><topic>Urinary Bladder Diseases - microbiology</topic><topic>Urinary Bladder Diseases - prevention & control</topic><topic>Urinary Tract Infections - microbiology</topic><topic>Uropathogenic E. coli</topic><topic>Uropathogenic Escherichia coli - drug effects</topic><topic>Uropathogenic Escherichia coli - pathogenicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sarshar, S.</creatorcontrib><creatorcontrib>Brandt, S.</creatorcontrib><creatorcontrib>Asadi Karam, M.R.</creatorcontrib><creatorcontrib>Habibi, M.</creatorcontrib><creatorcontrib>Bouzari, S.</creatorcontrib><creatorcontrib>Lechtenberg, M.</creatorcontrib><creatorcontrib>Dobrindt, U.</creatorcontrib><creatorcontrib>Qin, X.</creatorcontrib><creatorcontrib>Goycoolea, F.M.</creatorcontrib><creatorcontrib>Hensel, A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Phytomedicine (Stuttgart)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sarshar, S.</au><au>Brandt, S.</au><au>Asadi Karam, M.R.</au><au>Habibi, M.</au><au>Bouzari, S.</au><au>Lechtenberg, M.</au><au>Dobrindt, U.</au><au>Qin, X.</au><au>Goycoolea, F.M.</au><au>Hensel, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aqueous extract from Orthosiphon stamineus leaves prevents bladder and kidney infection in mice</atitle><jtitle>Phytomedicine (Stuttgart)</jtitle><addtitle>Phytomedicine</addtitle><date>2017-05-15</date><risdate>2017</risdate><volume>28</volume><spage>1</spage><epage>9</epage><pages>1-9</pages><issn>0944-7113</issn><eissn>1618-095X</eissn><abstract>Extracts from the leaves of Orthosiphon stamineus are used in phytotherapy for treatment of uncomplicated urinary tract infections.
Evaluation of an aqueous extract against infection with uropathogenic Escherichia coli in vivo; investigation of underlying microbiological mechanisms.
In vivo studies in mice and in vitro investigations on cytotoxicity, antiadhesive potential, influence on bacterial gene expression and quorum sensing.
Extract OWE was prepared by hot water extraction. For in vivo studies BALB/c mice were used in an UPEC infection model. The effect of OWE on bacterial load in bladder/kidney tissue was monitored in pre- and posttreatment. Cytotoxicity of OWE against different UPEC strains, T24 bladder/A498 kidney cells, gene expression analysis, monitoring of phenotypic motility and quorum sensing was investigated by standard methods of microbiology.
OWE was quantified (UHPLC) according to the content of rosmarinic acid, cichoric acid, caffeic acid. Three- and 5-day treatment of animals with OWE (750mg/kg) after transurethral infection with UPEC CFT073 reduced the bacterial load in bladder and kidney, similar to norfloxacin. Four- and 7-day pretreatment of mice prior to the infection with UPEC NU14 reduced bacterial bladder colonization. In vitro investigations indicated that OWE (≤2mg/ml) has no cytotoxic or proliferation-inhibiting activity against different UPEC strains as well as against T24 bladder and A498 kidney cells. OWE exerts a dose dependent antiadhesive activity against UPEC strains NU14 and UTI89. OWE reduced gene expression of fimH, but evoked increase of the expression of motility/fitness gene fliC. Increase of bacterial motility on gene level was confirmed by a changed bacterial phenotype by an increased bacterial motility in soft agar assay. OWE inhibited in a concentration-dependent manner bacterial quorum sensing.
OWE is assessed as a strong antiadhesive plant extract for which the traditional use in phytotherapy for UTI might be justified.
[Display omitted]</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>28478807</pmid><doi>10.1016/j.phymed.2017.02.009</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adhesion Animals Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Antiadhesion Disease Models, Animal Epithelial Cells - drug effects Escherichia coli Infections - drug therapy Escherichia coli Infections - prevention & control Female Humans Kidney Diseases - microbiology Kidney Diseases - pathology Kidney Diseases - prevention & control Mice Mice, Inbred BALB C Orthosiphon - chemistry Orthosiphon stamineus Plant Extracts - chemistry Plant Extracts - pharmacology Plant Leaves - chemistry Quorum sensing UPEC Urinary Bladder Diseases - microbiology Urinary Bladder Diseases - prevention & control Urinary Tract Infections - microbiology Uropathogenic E. coli Uropathogenic Escherichia coli - drug effects Uropathogenic Escherichia coli - pathogenicity |
| title | Aqueous extract from Orthosiphon stamineus leaves prevents bladder and kidney infection in mice |
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