Optimization of cord blood unit sterility testing: impact of dilution, analysis delay, and inhibitory substances

BACKGROUND Different methods are used by cord blood banks to prepare samples for sterility testing. Suboptimal methods can result in the release of contaminated products. In our organization, samples are prepared by diluting the final product in RPMI‐1640 medium. In this work, we have compared our m...

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Veröffentlicht in:	Transfusion (Philadelphia, Pa.) Pa.), 2017-08, Vol.57 (8), p.1956-1967
Hauptverfasser:	Girard, Mélissa, Laforce‐Lavoie, Audrey, de Grandmont, Marie Joëlle, Cayer, Marie‐Pierre, Fournier, Diane, Delage, Gilles, Thibault, Louis
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Bacterial Load - methods Bacterial Load - standards Bacteriological Techniques - methods Blood Blood Banking - methods Blood Banks - standards Blood cells Blood Specimen Collection - methods Bottles Cell culture Contamination Cord blood Design optimization Dilution E coli Erythrocytes Fetal Blood - microbiology Humans Impact analysis Indicator Dilution Techniques Infertility - blood Inoculation Klebsiella Plasmas (physics) Sample preparation Staphylococcus epidermidis Stem cells Sterility Sterility testing Temperature Time Factors
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