Shaved margin histopathology and imprint cytology for assessment of excision in canine mast cell tumors and soft tissue sarcomas
Objective To determine the feasibility and agreement of margin assessment by imprint cytology, shaved margin histopathology, and radial section histopathology in canine cutaneous and subcutaneous mast cell tumors (MCT) and soft tissue sarcomas (STS). Study design Prospective clinical study. Sample p...
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| Veröffentlicht in: | Veterinary surgery 2017-08, Vol.46 (6), p.879-885 |
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| creator | Milovancev, Milan Townsend, Kaitlin L. Gorman, Elena Bracha, Shay Curran, Katie Russell, Duncan S. |
| description | Objective
To determine the feasibility and agreement of margin assessment by imprint cytology, shaved margin histopathology, and radial section histopathology in canine cutaneous and subcutaneous mast cell tumors (MCT) and soft tissue sarcomas (STS).
Study design
Prospective clinical study.
Sample population
Three hundred and forty margins from 72 excised tumors (52 MCT and 20 STS) in 54 client‐owned dogs.
Methods
Imprint cytology samples were acquired by pressing glass slides to the cut surgical margin of the freshly excised surgical specimen. Shaved margin samples were obtained from the patient wound bed using a scalpel immediately prior to closure. Radial section histopathology was performed as part of routine histopathologic processing. All margins were assessed as either positive or negative for presence of tumor cells at the surgical margin. Agreement among methods was calculated using Fleiss Kappa coefficients and an association of method, margin direction, and tumor type with positive margin status was evaluated using a general linear mixed model.
Results
Positive margin detection rates differed for MCT (imprint cytology 21%, radial section histopathology 9%, and shaved margin histopathology 3%; P |
| doi_str_mv | 10.1111/vsu.12668 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1894520406</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1894520406</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3538-9670b467ae25dfcf7d5f6f4d00366b77f862b72fc162b3ab8eef3abc3f50cd473</originalsourceid><addsrcrecordid>eNp1kc1O3DAUha2qqDNAF32BylI3ZRGw458kS4TKj4TEYgrqznIcmzFK4sE3GZgdj45nMnRRqd4c6erzp6t7EPpGySlN72wN4ynNpSw_oTkVLM8qQf58RnNCJc0Yr6oZOgR4IoRUnLMvaJaXXBJOqzl6Wyz12ja40_HR93jpYQgrPSxDGx43WPcN9t0q-n7AZjNMQxci1gAWoLNpHhy2r8aDDz1OBqN739vkg_TFti0exi5E2KkguAEPHmC0GHQ0IVHH6MDpFuzXfR6h-8tfvy-us9u7q5uL89vMMMHKrJIFqbkstM1F44wrGuGk4w0hTMq6KFwp87rInaEpma5La10Kw5wgpuEFO0I_J-8qhufRwqA6D9sFdW_DCIqWFRc54UQm9Mc_6FMYY5-2U7TKeSkEEWWiTibKxAAQrVPpTumMG0WJ2taiUi1qV0tiv--NY93Z5i_50UMCzibgxbd283-TeljcT8p33JiZ9w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1924855058</pqid></control><display><type>article</type><title>Shaved margin histopathology and imprint cytology for assessment of excision in canine mast cell tumors and soft tissue sarcomas</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Milovancev, Milan ; Townsend, Kaitlin L. ; Gorman, Elena ; Bracha, Shay ; Curran, Katie ; Russell, Duncan S.</creator><creatorcontrib>Milovancev, Milan ; Townsend, Kaitlin L. ; Gorman, Elena ; Bracha, Shay ; Curran, Katie ; Russell, Duncan S.</creatorcontrib><description>Objective
To determine the feasibility and agreement of margin assessment by imprint cytology, shaved margin histopathology, and radial section histopathology in canine cutaneous and subcutaneous mast cell tumors (MCT) and soft tissue sarcomas (STS).
Study design
Prospective clinical study.
Sample population
Three hundred and forty margins from 72 excised tumors (52 MCT and 20 STS) in 54 client‐owned dogs.
Methods
Imprint cytology samples were acquired by pressing glass slides to the cut surgical margin of the freshly excised surgical specimen. Shaved margin samples were obtained from the patient wound bed using a scalpel immediately prior to closure. Radial section histopathology was performed as part of routine histopathologic processing. All margins were assessed as either positive or negative for presence of tumor cells at the surgical margin. Agreement among methods was calculated using Fleiss Kappa coefficients and an association of method, margin direction, and tumor type with positive margin status was evaluated using a general linear mixed model.
Results
Positive margin detection rates differed for MCT (imprint cytology 21%, radial section histopathology 9%, and shaved margin histopathology 3%; P < .0001) but not for STS. Intermethod agreement was poor (Fleiss Kappa = 0.051 and 0.176 for MCT and STS, respectively). Margin direction did not influence margin status for either tumor type.
Conclusion
Imprint cytology and shaved margin histopathology are feasible, but their results are frequently disparate from routine radial section histopathology. Future studies are needed to evaluate the correlation of each method with local recurrence rates.</description><identifier>ISSN: 0161-3499</identifier><identifier>EISSN: 1532-950X</identifier><identifier>DOI: 10.1111/vsu.12668</identifier><identifier>PMID: 28460419</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Animals ; Cellular biology ; Cytodiagnosis - methods ; Cytodiagnosis - veterinary ; Cytology ; Dog Diseases - surgery ; Dogs ; Feasibility studies ; Female ; Histopathology ; Male ; Mastocytoma - surgery ; Mastocytoma - veterinary ; Population studies ; Prospective Studies ; Sampling methods ; Sarcoma - surgery ; Sarcoma - veterinary ; Surgery ; Surgery, Veterinary - methods ; Tumor cells ; Tumors ; Wounds</subject><ispartof>Veterinary surgery, 2017-08, Vol.46 (6), p.879-885</ispartof><rights>2017 The American College of Veterinary Surgeons</rights><rights>2017 The American College of Veterinary Surgeons.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3538-9670b467ae25dfcf7d5f6f4d00366b77f862b72fc162b3ab8eef3abc3f50cd473</citedby><cites>FETCH-LOGICAL-c3538-9670b467ae25dfcf7d5f6f4d00366b77f862b72fc162b3ab8eef3abc3f50cd473</cites><orcidid>0000-0002-0556-4409</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fvsu.12668$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fvsu.12668$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28460419$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Milovancev, Milan</creatorcontrib><creatorcontrib>Townsend, Kaitlin L.</creatorcontrib><creatorcontrib>Gorman, Elena</creatorcontrib><creatorcontrib>Bracha, Shay</creatorcontrib><creatorcontrib>Curran, Katie</creatorcontrib><creatorcontrib>Russell, Duncan S.</creatorcontrib><title>Shaved margin histopathology and imprint cytology for assessment of excision in canine mast cell tumors and soft tissue sarcomas</title><title>Veterinary surgery</title><addtitle>Vet Surg</addtitle><description>Objective
To determine the feasibility and agreement of margin assessment by imprint cytology, shaved margin histopathology, and radial section histopathology in canine cutaneous and subcutaneous mast cell tumors (MCT) and soft tissue sarcomas (STS).
Study design
Prospective clinical study.
Sample population
Three hundred and forty margins from 72 excised tumors (52 MCT and 20 STS) in 54 client‐owned dogs.
Methods
Imprint cytology samples were acquired by pressing glass slides to the cut surgical margin of the freshly excised surgical specimen. Shaved margin samples were obtained from the patient wound bed using a scalpel immediately prior to closure. Radial section histopathology was performed as part of routine histopathologic processing. All margins were assessed as either positive or negative for presence of tumor cells at the surgical margin. Agreement among methods was calculated using Fleiss Kappa coefficients and an association of method, margin direction, and tumor type with positive margin status was evaluated using a general linear mixed model.
Results
Positive margin detection rates differed for MCT (imprint cytology 21%, radial section histopathology 9%, and shaved margin histopathology 3%; P < .0001) but not for STS. Intermethod agreement was poor (Fleiss Kappa = 0.051 and 0.176 for MCT and STS, respectively). Margin direction did not influence margin status for either tumor type.
Conclusion
Imprint cytology and shaved margin histopathology are feasible, but their results are frequently disparate from routine radial section histopathology. Future studies are needed to evaluate the correlation of each method with local recurrence rates.</description><subject>Animals</subject><subject>Cellular biology</subject><subject>Cytodiagnosis - methods</subject><subject>Cytodiagnosis - veterinary</subject><subject>Cytology</subject><subject>Dog Diseases - surgery</subject><subject>Dogs</subject><subject>Feasibility studies</subject><subject>Female</subject><subject>Histopathology</subject><subject>Male</subject><subject>Mastocytoma - surgery</subject><subject>Mastocytoma - veterinary</subject><subject>Population studies</subject><subject>Prospective Studies</subject><subject>Sampling methods</subject><subject>Sarcoma - surgery</subject><subject>Sarcoma - veterinary</subject><subject>Surgery</subject><subject>Surgery, Veterinary - methods</subject><subject>Tumor cells</subject><subject>Tumors</subject><subject>Wounds</subject><issn>0161-3499</issn><issn>1532-950X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1O3DAUha2qqDNAF32BylI3ZRGw458kS4TKj4TEYgrqznIcmzFK4sE3GZgdj45nMnRRqd4c6erzp6t7EPpGySlN72wN4ynNpSw_oTkVLM8qQf58RnNCJc0Yr6oZOgR4IoRUnLMvaJaXXBJOqzl6Wyz12ja40_HR93jpYQgrPSxDGx43WPcN9t0q-n7AZjNMQxci1gAWoLNpHhy2r8aDDz1OBqN739vkg_TFti0exi5E2KkguAEPHmC0GHQ0IVHH6MDpFuzXfR6h-8tfvy-us9u7q5uL89vMMMHKrJIFqbkstM1F44wrGuGk4w0hTMq6KFwp87rInaEpma5La10Kw5wgpuEFO0I_J-8qhufRwqA6D9sFdW_DCIqWFRc54UQm9Mc_6FMYY5-2U7TKeSkEEWWiTibKxAAQrVPpTumMG0WJ2taiUi1qV0tiv--NY93Z5i_50UMCzibgxbd283-TeljcT8p33JiZ9w</recordid><startdate>201708</startdate><enddate>201708</enddate><creator>Milovancev, Milan</creator><creator>Townsend, Kaitlin L.</creator><creator>Gorman, Elena</creator><creator>Bracha, Shay</creator><creator>Curran, Katie</creator><creator>Russell, Duncan S.</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0556-4409</orcidid></search><sort><creationdate>201708</creationdate><title>Shaved margin histopathology and imprint cytology for assessment of excision in canine mast cell tumors and soft tissue sarcomas</title><author>Milovancev, Milan ; Townsend, Kaitlin L. ; Gorman, Elena ; Bracha, Shay ; Curran, Katie ; Russell, Duncan S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3538-9670b467ae25dfcf7d5f6f4d00366b77f862b72fc162b3ab8eef3abc3f50cd473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Cellular biology</topic><topic>Cytodiagnosis - methods</topic><topic>Cytodiagnosis - veterinary</topic><topic>Cytology</topic><topic>Dog Diseases - surgery</topic><topic>Dogs</topic><topic>Feasibility studies</topic><topic>Female</topic><topic>Histopathology</topic><topic>Male</topic><topic>Mastocytoma - surgery</topic><topic>Mastocytoma - veterinary</topic><topic>Population studies</topic><topic>Prospective Studies</topic><topic>Sampling methods</topic><topic>Sarcoma - surgery</topic><topic>Sarcoma - veterinary</topic><topic>Surgery</topic><topic>Surgery, Veterinary - methods</topic><topic>Tumor cells</topic><topic>Tumors</topic><topic>Wounds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Milovancev, Milan</creatorcontrib><creatorcontrib>Townsend, Kaitlin L.</creatorcontrib><creatorcontrib>Gorman, Elena</creatorcontrib><creatorcontrib>Bracha, Shay</creatorcontrib><creatorcontrib>Curran, Katie</creatorcontrib><creatorcontrib>Russell, Duncan S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Milovancev, Milan</au><au>Townsend, Kaitlin L.</au><au>Gorman, Elena</au><au>Bracha, Shay</au><au>Curran, Katie</au><au>Russell, Duncan S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Shaved margin histopathology and imprint cytology for assessment of excision in canine mast cell tumors and soft tissue sarcomas</atitle><jtitle>Veterinary surgery</jtitle><addtitle>Vet Surg</addtitle><date>2017-08</date><risdate>2017</risdate><volume>46</volume><issue>6</issue><spage>879</spage><epage>885</epage><pages>879-885</pages><issn>0161-3499</issn><eissn>1532-950X</eissn><abstract>Objective
To determine the feasibility and agreement of margin assessment by imprint cytology, shaved margin histopathology, and radial section histopathology in canine cutaneous and subcutaneous mast cell tumors (MCT) and soft tissue sarcomas (STS).
Study design
Prospective clinical study.
Sample population
Three hundred and forty margins from 72 excised tumors (52 MCT and 20 STS) in 54 client‐owned dogs.
Methods
Imprint cytology samples were acquired by pressing glass slides to the cut surgical margin of the freshly excised surgical specimen. Shaved margin samples were obtained from the patient wound bed using a scalpel immediately prior to closure. Radial section histopathology was performed as part of routine histopathologic processing. All margins were assessed as either positive or negative for presence of tumor cells at the surgical margin. Agreement among methods was calculated using Fleiss Kappa coefficients and an association of method, margin direction, and tumor type with positive margin status was evaluated using a general linear mixed model.
Results
Positive margin detection rates differed for MCT (imprint cytology 21%, radial section histopathology 9%, and shaved margin histopathology 3%; P < .0001) but not for STS. Intermethod agreement was poor (Fleiss Kappa = 0.051 and 0.176 for MCT and STS, respectively). Margin direction did not influence margin status for either tumor type.
Conclusion
Imprint cytology and shaved margin histopathology are feasible, but their results are frequently disparate from routine radial section histopathology. Future studies are needed to evaluate the correlation of each method with local recurrence rates.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>28460419</pmid><doi>10.1111/vsu.12668</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-0556-4409</orcidid></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Cellular biology Cytodiagnosis - methods Cytodiagnosis - veterinary Cytology Dog Diseases - surgery Dogs Feasibility studies Female Histopathology Male Mastocytoma - surgery Mastocytoma - veterinary Population studies Prospective Studies Sampling methods Sarcoma - surgery Sarcoma - veterinary Surgery Surgery, Veterinary - methods Tumor cells Tumors Wounds |
| title | Shaved margin histopathology and imprint cytology for assessment of excision in canine mast cell tumors and soft tissue sarcomas |
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