Purification of β-mannanase derived from Bacillus subtilis ATCC 11774 using ionic liquid as adjuvant in aqueous two-phase system

•First report on ATPS using ionic liquid as adjuvant to purify β-mannanase.•Optimisation of phase composition in ATPS using RSM.•The optimum results were obtained from PEG 6000/citrate with 3% ionic liquid.•New finding on ionic liquid as adjuvant in ATPS greatly increased recovery yield. The partiti...

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Veröffentlicht in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2017-06, Vol.1055-1056, p.104-112
Hauptverfasser:	Aziz, Nur Fazrin Husna Abdul, Abbasiliasi, Sahar, Ng, Hui Suan, Phapugrangkul, Pongsathon, Bakar, Mohamad Hafizi Abu, Tam, Yew Joon, Tan, Joo Shun
Format:	Artikel
Sprache:	eng
Schlagworte:	Aqueous two-phase system Bacillus subtilis - chemistry Bacillus subtilis - enzymology beta-Mannosidase - chemistry beta-Mannosidase - isolation & purification Citrates - chemistry Electrophoresis, Polyacrylamide Gel Imidazoles - chemistry Ionic liquid Ionic Liquids - chemistry Polyethylene glycol Polyethylene Glycols - chemistry Purification Water - chemistry β-Mannanase
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