Stimulated Raman scattering microscopy and spectroscopy with a rapid scanning optical delay line
Stimulated Raman scattering (SRS) microscopy that is capable of both high-speed imaging and rapid spectroscopy will be advantageous for detailed chemical analysis of heterogeneous biological specimens. We have developed a system based on spectral focusing SRS technology with the integration of a rap...
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Veröffentlicht in: | Optics letters 2017-02, Vol.42 (4), p.659-662 |
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creator | He, Ruoyu Liu, Zhiping Xu, Yongkui Huang, Wei Ma, Hong Ji, Minbiao |
description | Stimulated Raman scattering (SRS) microscopy that is capable of both high-speed imaging and rapid spectroscopy will be advantageous for detailed chemical analysis of heterogeneous biological specimens. We have developed a system based on spectral focusing SRS technology with the integration of a rapid scanning optical delay line, which allows continuous tuning of SRS spectra by scanning a galvo mirror. We demonstrate SRS spectral measurements of dimethyl sulfoxide solution at low concentrations and multi-color imaging of rice pollens and HeLa cells with line-by-line delay tuning to reduce motion artifacts, as well as fast acquisition of SRS spectra at specific regions of interest. |
doi_str_mv | 10.1364/OL.42.000659 |
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We demonstrate SRS spectral measurements of dimethyl sulfoxide solution at low concentrations and multi-color imaging of rice pollens and HeLa cells with line-by-line delay tuning to reduce motion artifacts, as well as fast acquisition of SRS spectra at specific regions of interest.</description><subject>Delay lines</subject><subject>Focusing</subject><subject>Imaging</subject><subject>Microscopy</subject><subject>Raman scattering</subject><subject>Scanning</subject><subject>Spectral lines</subject><subject>Tuning</subject><issn>0146-9592</issn><issn>1539-4794</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNqNkDtPwzAYRS0EoqWwMSOPDKT4FcffiBAvKVIlHrNxYgeM8iJ2hPrvadSWmelKV-fe4SB0TsmScimuV_lSsCUhRKZwgOY05ZCIDMQhmhMqZAIpsBk6CeFrYjLOj9GMKQpKAZuj95fom7E20Vn8bBrT4lCaGN3g2w_c-HLoQtn1a2xai0Pvyrgvfnz8xAYPpvd22rTttOj66EtTY-tqs8a1b90pOqpMHdzZLhfo7f7u9fYxyVcPT7c3eVJyJmIiiBPGCgLUKsZIZRV1IHlhZCWUVJklTBUcqqySGQBLaZaSiqm0IIUTjFO-QJfb337ovkcXom58KF1dm9Z1Y9BUAQfCJYd_oFJJoBTEBr3aopOIMLhK94NvzLDWlOhJv17lWjC91b_BL3bPY9E4-wfvffNfnzt_eg</recordid><startdate>20170215</startdate><enddate>20170215</enddate><creator>He, Ruoyu</creator><creator>Liu, Zhiping</creator><creator>Xu, Yongkui</creator><creator>Huang, Wei</creator><creator>Ma, Hong</creator><creator>Ji, Minbiao</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SP</scope><scope>7U5</scope><scope>8FD</scope><scope>H8D</scope><scope>L7M</scope></search><sort><creationdate>20170215</creationdate><title>Stimulated Raman scattering microscopy and spectroscopy with a rapid scanning optical delay line</title><author>He, Ruoyu ; Liu, Zhiping ; Xu, Yongkui ; Huang, Wei ; Ma, Hong ; Ji, Minbiao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c324t-40e4ad4091d8220fd81e963ba6f48687d028b39f7f6799251750f285b0be42313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Delay lines</topic><topic>Focusing</topic><topic>Imaging</topic><topic>Microscopy</topic><topic>Raman scattering</topic><topic>Scanning</topic><topic>Spectral lines</topic><topic>Tuning</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>He, Ruoyu</creatorcontrib><creatorcontrib>Liu, Zhiping</creatorcontrib><creatorcontrib>Xu, Yongkui</creatorcontrib><creatorcontrib>Huang, Wei</creatorcontrib><creatorcontrib>Ma, Hong</creatorcontrib><creatorcontrib>Ji, Minbiao</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Aerospace Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Optics letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>He, Ruoyu</au><au>Liu, Zhiping</au><au>Xu, Yongkui</au><au>Huang, Wei</au><au>Ma, Hong</au><au>Ji, Minbiao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stimulated Raman scattering microscopy and spectroscopy with a rapid scanning optical delay line</atitle><jtitle>Optics letters</jtitle><addtitle>Opt Lett</addtitle><date>2017-02-15</date><risdate>2017</risdate><volume>42</volume><issue>4</issue><spage>659</spage><epage>662</epage><pages>659-662</pages><issn>0146-9592</issn><eissn>1539-4794</eissn><abstract>Stimulated Raman scattering (SRS) microscopy that is capable of both high-speed imaging and rapid spectroscopy will be advantageous for detailed chemical analysis of heterogeneous biological specimens. 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subjects | Delay lines Focusing Imaging Microscopy Raman scattering Scanning Spectral lines Tuning |
title | Stimulated Raman scattering microscopy and spectroscopy with a rapid scanning optical delay line |
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