Transcription of 4'-thioDNA templates to natural RNA in vitro and in mammalian cells
Synthetic chemically modified nucleic acids, which are compatible with DNA/RNA polymerases, have great potential as a genetic material for synthetic biological studies. A comprehensive analysis of the transcription of 4'-thioDNA templates to natural RNA is herein reported. The modifications of...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2015-05, Vol.51 (37), p.7887-7890 |
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| container_issue | 37 |
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| container_title | Chemical communications (Cambridge, England) |
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| creator | Maruyama, Hideto Furukawa, Kazuhiro Kamiya, Hiroyuki Minakawa, Noriaki Matsuda, Akira |
| description | Synthetic chemically modified nucleic acids, which are compatible with DNA/RNA polymerases, have great potential as a genetic material for synthetic biological studies. A comprehensive analysis of the transcription of 4'-thioDNA templates to natural RNA is herein reported. The modifications of DNA with 2'-deoxy-4'-thionucleosides, so-called 4'-thioDNA, were found to act as templates for transcription with RNA polymerases both in vitro and in mammalian cells. |
| doi_str_mv | 10.1039/c4cc08862j |
| format | Article |
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A comprehensive analysis of the transcription of 4'-thioDNA templates to natural RNA is herein reported. 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A comprehensive analysis of the transcription of 4'-thioDNA templates to natural RNA is herein reported. 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| ispartof | Chemical communications (Cambridge, England), 2015-05, Vol.51 (37), p.7887-7890 |
| issn | 1359-7345 1364-548X |
| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Animals Biological materials Compatibility Deoxyribonucleic acid DNA - chemistry DNA - genetics DNA - metabolism DNA-Directed RNA Polymerases - metabolism Genetics In Vitro Techniques Mammals Mice NIH 3T3 Cells Nucleic acids Ribonucleic acids RNA - biosynthesis RNA - genetics Templates, Genetic Thiophenes - chemistry Thiophenes - metabolism Transcription, Genetic |
| title | Transcription of 4'-thioDNA templates to natural RNA in vitro and in mammalian cells |
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