Collagen labelling with an azide-proline chemical reporter in live cells

We have developed a strategy for selective imaging of collagen in live foetal ovine osteoblasts. Our approach involves the incorporation of an azide-tagged proline in the biosynthesis of collagen followed by labelling using a strain-promoted [3+2] azide-alkyne cycloaddition reaction.

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Bibliographische Detailangaben
Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2015-03, Vol.51 (25), p.5250-5252
Hauptverfasser:	Amgarten, Beatrice, Rajan, Rakesh, Martínez-Sáez, Nuria, Oliveira, Bruno L, Albuquerque, Inês S, Brooks, Roger A, Reid, David G, Duer, Melinda J, Bernardes, Gonçalo J L
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkynes - chemistry Azides - chemistry Biosynthesis Cell Survival Cells, Cultured Collagen - analysis Collagens Cyclization Cycloaddition Humans Imaging Labelling Osteoblasts Osteoblasts - chemistry Osteoblasts - cytology Proline Proline - chemistry Strategy
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Beschreibung
Zusammenfassung:	We have developed a strategy for selective imaging of collagen in live foetal ovine osteoblasts. Our approach involves the incorporation of an azide-tagged proline in the biosynthesis of collagen followed by labelling using a strain-promoted [3+2] azide-alkyne cycloaddition reaction.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c4cc07974d