Multiple GO-SELEX for efficient screening of flexible aptamers
We describe a simple, high-speed, high-throughput aptamer screening for a group of small molecules using graphene oxide (simple Multi-GO-SELEX) without immobilizing targets. The affinities of ten different ssDNA aptamers successfully obtained for three pesticides were in the range of 10-100 nM. Besi...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2014-09, Vol.50 (72), p.10513-10516 |
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| container_issue | 72 |
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| container_title | Chemical communications (Cambridge, England) |
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| creator | Nguyen, Van-Thuan Kwon, Young Seop Kim, Jae Hoon Gu, Man Bock |
| description | We describe a simple, high-speed, high-throughput aptamer screening for a group of small molecules using graphene oxide (simple Multi-GO-SELEX) without immobilizing targets. The affinities of ten different ssDNA aptamers successfully obtained for three pesticides were in the range of 10-100 nM. Besides a specific aptamer for each target, we found a couple of flexible multi-target aptamers, which can bind with 2 or 3 different molecules. These flexible aptamers developed for binding with a mixture of targets are not only significant for the rapid screening of a group of small molecules but also offer great promise for aptamer-based biosensor applications. |
| doi_str_mv | 10.1039/c4cc03953j |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 1359-7345 |
| ispartof | Chemical communications (Cambridge, England), 2014-09, Vol.50 (72), p.10513-10516 |
| issn | 1359-7345 1364-548X |
| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Affinity Aptamers, Nucleotide - chemistry Binding Biosensing Techniques Colorimetry Couples DNA, Single-Stranded - chemistry Gold - chemistry Graphene Graphite - chemistry High speed Metal Nanoparticles - chemistry Oxides Oxides - chemistry Pesticides Pesticides - analysis Screening SELEX Aptamer Technique |
| title | Multiple GO-SELEX for efficient screening of flexible aptamers |
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