Paper-based microfluidic system for tear electrolyte analysis

The analysis of tear constituents at point-of-care settings has a potential for early diagnosis of ocular disorders such as dry eye disease, low-cost screening, and surveillance of at-risk subjects. However, current minimally-invasive rapid tear analysis systems for point-of-care settings have been...

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Veröffentlicht in:Lab on a chip 2017-03, Vol.17 (6), p.1137-1148
Hauptverfasser: Yetisen, Ali K, Jiang, Nan, Tamayol, Ali, Ruiz-Esparza, Guillermo U, Zhang, Yu Shrike, Medina-Pando, Sofía, Gupta, Aditi, Wolffsohn, James S, Butt, Haider, Khademhosseini, Ali, Yun, Seok-Hyun
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container_end_page 1148
container_issue 6
container_start_page 1137
container_title Lab on a chip
container_volume 17
creator Yetisen, Ali K
Jiang, Nan
Tamayol, Ali
Ruiz-Esparza, Guillermo U
Zhang, Yu Shrike
Medina-Pando, Sofía
Gupta, Aditi
Wolffsohn, James S
Butt, Haider
Khademhosseini, Ali
Yun, Seok-Hyun
description The analysis of tear constituents at point-of-care settings has a potential for early diagnosis of ocular disorders such as dry eye disease, low-cost screening, and surveillance of at-risk subjects. However, current minimally-invasive rapid tear analysis systems for point-of-care settings have been limited to assessment of osmolarity or inflammatory markers and cannot differentiate between dry eye subclassifications. Here, we demonstrate a portable microfluidic system that allows quantitative analysis of electrolytes in the tear fluid that is suited for point-of-care settings. The microfluidic system consists of a capillary tube for sample collection, a reservoir for sample dilution, and a paper-based microfluidic device for electrolyte analysis. The sensing regions are functionalized with fluorescent crown ethers, o-acetanisidide, and seminaphtorhodafluor that are sensitive to mono- and divalent electrolytes, and their fluorescence outputs are measured with a smartphone readout device. The measured sensitivity values of Na , K , Ca ions and pH in artificial tear fluid were matched with the known ion concentrations within the physiological range. The microfluidic system was tested with samples having different ionic concentrations, demonstrating the feasibility for the detection of early-stage dry eye, differential diagnosis of dry eye sub-types, and their severity staging.
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source MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection
subjects Devices
Dry Eye Syndromes
Drying
Electrolytes
Electrolytes - analysis
Equipment Design
Eyes
Fluorescence
Humans
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Microfluidics
Potassium - analysis
Sodium - analysis
Systems analysis
Tearing
Tears - chemistry
title Paper-based microfluidic system for tear electrolyte analysis
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