Silencing of Agrobacterium tumefaciens oncogenes ipt and iaaM induces resistance to crown gall disease in plum but not in apricot
BACKGROUND In this study, two vectors with short‐length chimeric transgenes were used to produce Prunus rootstocks resistant to crown gall disease through RNA‐interference‐mediated gene silencing of the Agrobacterium tumefaciens oncogenes ipt and iaaM. RESULTS Transgenic plum and apricot lines were...
Gespeichert in:
| Veröffentlicht in: | Pest management science 2017-10, Vol.73 (10), p.2163-2173 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2173 |
|---|---|
| container_issue | 10 |
| container_start_page | 2163 |
| container_title | Pest management science |
| container_volume | 73 |
| creator | Alburquerque, Nuria Faize, Lydia Burgos, Lorenzo |
| description | BACKGROUND
In this study, two vectors with short‐length chimeric transgenes were used to produce Prunus rootstocks resistant to crown gall disease through RNA‐interference‐mediated gene silencing of the Agrobacterium tumefaciens oncogenes ipt and iaaM.
RESULTS
Transgenic plum and apricot lines were produced with efficiencies of up to 7.7 and 1.1% respectively. An in vitro evaluation method allowed identification of susceptible lines and reduction in the number of lines to be evaluated in the greenhouse. Five transgenic plum lines, expressing transgene‐derived small interfering RNA (siRNA) and low levels of transgene hairpin RNA (hpRNA), showed a significant reduction in the development of the disease after infection with Agrobacterium strains C58 and A281 under greenhouse conditions. However, unexpectedly, all transgenic apricot lines were gall susceptible. The infection of apricot plants with a binary vector containing only the 6b oncogene demonstrated that the expression of this gene is involved in the induction of tumours in the apricot species.
CONCLUSION
RNAi‐mediated gene silencing can be used for inducing crown gall resistance in plum rootstocks. These could be used to graft non‐genetically modified commercial fruit cultivars reducing, or eliminating, the disease symptoms. © 2017 Society of Chemical Industry |
| doi_str_mv | 10.1002/ps.4600 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1893552503</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1893552503</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3450-752756bea0e703f6d67ff20f4352b967469ba6711f15e643820fcbfe7bfad6ab3</originalsourceid><addsrcrecordid>eNp1kU1rFTEUhoNY7IfiP5CACwtya5JJMpNlKbYKFYUquAtJ5uSSMpOMSYbSpf-8ud7aheAqycnDw3l5EXpNyRklhH1YyhmXhDxDR1QwueFKDc-f7sPPQ3Rcyi0hRCnFXqBDNnCuGKFH6PdNmCC6ELc4eXy-zckaVyGHdcZ1ncEbFyAWnKJLW4hQcFgqNnHEwZgvOMRxdW2YoYRSTXSAa8Iup7uIt2aa8BgKmAINxMvUnHatOKa6e5slB5fqS3TgzVTg1eN5gn5cfvx-8Wlz_fXq88X59cZ1XJBNL1gvpAVDoCedl6PsvWfE804wq2TPpbJG9pR6KkDybmh_znrorTejNLY7Qad775LTrxVK1XMoDqbJREhr0XRQnRBMkK6hb_9Bb9OaY9tOU9VxKgmjvFHv9lSLW0oGr1ug2eR7TYnetaKXonetNPLNo2-1M4xP3N8aGvB-D9y1Nu7_59Hfbv7oHgCFQZXt</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1934160214</pqid></control><display><type>article</type><title>Silencing of Agrobacterium tumefaciens oncogenes ipt and iaaM induces resistance to crown gall disease in plum but not in apricot</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Alburquerque, Nuria ; Faize, Lydia ; Burgos, Lorenzo</creator><creatorcontrib>Alburquerque, Nuria ; Faize, Lydia ; Burgos, Lorenzo</creatorcontrib><description>BACKGROUND
In this study, two vectors with short‐length chimeric transgenes were used to produce Prunus rootstocks resistant to crown gall disease through RNA‐interference‐mediated gene silencing of the Agrobacterium tumefaciens oncogenes ipt and iaaM.
RESULTS
Transgenic plum and apricot lines were produced with efficiencies of up to 7.7 and 1.1% respectively. An in vitro evaluation method allowed identification of susceptible lines and reduction in the number of lines to be evaluated in the greenhouse. Five transgenic plum lines, expressing transgene‐derived small interfering RNA (siRNA) and low levels of transgene hairpin RNA (hpRNA), showed a significant reduction in the development of the disease after infection with Agrobacterium strains C58 and A281 under greenhouse conditions. However, unexpectedly, all transgenic apricot lines were gall susceptible. The infection of apricot plants with a binary vector containing only the 6b oncogene demonstrated that the expression of this gene is involved in the induction of tumours in the apricot species.
CONCLUSION
RNAi‐mediated gene silencing can be used for inducing crown gall resistance in plum rootstocks. These could be used to graft non‐genetically modified commercial fruit cultivars reducing, or eliminating, the disease symptoms. © 2017 Society of Chemical Industry</description><identifier>ISSN: 1526-498X</identifier><identifier>EISSN: 1526-4998</identifier><identifier>DOI: 10.1002/ps.4600</identifier><identifier>PMID: 28449201</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Agrobacterium tumefaciens ; Agrobacterium tumefaciens - genetics ; apricot ; Apricots ; bacterial plant disease ; Bacterial Proteins - genetics ; Canker ; Crown gall ; Cultivars ; Disease Resistance ; Gall ; Gene expression ; Gene Silencing ; Genetic modification ; Greenhouses ; In vitro methods and tests ; Infections ; oncogene silencing ; Oncogenes ; Oncogenes - genetics ; Plant bacterial diseases ; Plant Tumors - microbiology ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - microbiology ; plum ; Prunus ; Prunus armeniaca - genetics ; Prunus armeniaca - microbiology ; Prunus domestica - genetics ; Prunus domestica - microbiology ; Reduction ; Ribonucleic acid ; RNA ; RNA-mediated interference ; Rootstocks ; siRNA ; Transgenes ; Transgenic plants ; Tumors</subject><ispartof>Pest management science, 2017-10, Vol.73 (10), p.2163-2173</ispartof><rights>2017 Society of Chemical Industry</rights><rights>2017 Society of Chemical Industry.</rights><rights>Copyright © 2017 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3450-752756bea0e703f6d67ff20f4352b967469ba6711f15e643820fcbfe7bfad6ab3</citedby><cites>FETCH-LOGICAL-c3450-752756bea0e703f6d67ff20f4352b967469ba6711f15e643820fcbfe7bfad6ab3</cites><orcidid>0000-0003-2399-8232 ; 0000-0002-7770-5100</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fps.4600$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fps.4600$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27911,27912,45561,45562</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28449201$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alburquerque, Nuria</creatorcontrib><creatorcontrib>Faize, Lydia</creatorcontrib><creatorcontrib>Burgos, Lorenzo</creatorcontrib><title>Silencing of Agrobacterium tumefaciens oncogenes ipt and iaaM induces resistance to crown gall disease in plum but not in apricot</title><title>Pest management science</title><addtitle>Pest Manag Sci</addtitle><description>BACKGROUND
In this study, two vectors with short‐length chimeric transgenes were used to produce Prunus rootstocks resistant to crown gall disease through RNA‐interference‐mediated gene silencing of the Agrobacterium tumefaciens oncogenes ipt and iaaM.
RESULTS
Transgenic plum and apricot lines were produced with efficiencies of up to 7.7 and 1.1% respectively. An in vitro evaluation method allowed identification of susceptible lines and reduction in the number of lines to be evaluated in the greenhouse. Five transgenic plum lines, expressing transgene‐derived small interfering RNA (siRNA) and low levels of transgene hairpin RNA (hpRNA), showed a significant reduction in the development of the disease after infection with Agrobacterium strains C58 and A281 under greenhouse conditions. However, unexpectedly, all transgenic apricot lines were gall susceptible. The infection of apricot plants with a binary vector containing only the 6b oncogene demonstrated that the expression of this gene is involved in the induction of tumours in the apricot species.
CONCLUSION
RNAi‐mediated gene silencing can be used for inducing crown gall resistance in plum rootstocks. These could be used to graft non‐genetically modified commercial fruit cultivars reducing, or eliminating, the disease symptoms. © 2017 Society of Chemical Industry</description><subject>Agrobacterium tumefaciens</subject><subject>Agrobacterium tumefaciens - genetics</subject><subject>apricot</subject><subject>Apricots</subject><subject>bacterial plant disease</subject><subject>Bacterial Proteins - genetics</subject><subject>Canker</subject><subject>Crown gall</subject><subject>Cultivars</subject><subject>Disease Resistance</subject><subject>Gall</subject><subject>Gene expression</subject><subject>Gene Silencing</subject><subject>Genetic modification</subject><subject>Greenhouses</subject><subject>In vitro methods and tests</subject><subject>Infections</subject><subject>oncogene silencing</subject><subject>Oncogenes</subject><subject>Oncogenes - genetics</subject><subject>Plant bacterial diseases</subject><subject>Plant Tumors - microbiology</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - microbiology</subject><subject>plum</subject><subject>Prunus</subject><subject>Prunus armeniaca - genetics</subject><subject>Prunus armeniaca - microbiology</subject><subject>Prunus domestica - genetics</subject><subject>Prunus domestica - microbiology</subject><subject>Reduction</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA-mediated interference</subject><subject>Rootstocks</subject><subject>siRNA</subject><subject>Transgenes</subject><subject>Transgenic plants</subject><subject>Tumors</subject><issn>1526-498X</issn><issn>1526-4998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1rFTEUhoNY7IfiP5CACwtya5JJMpNlKbYKFYUquAtJ5uSSMpOMSYbSpf-8ud7aheAqycnDw3l5EXpNyRklhH1YyhmXhDxDR1QwueFKDc-f7sPPQ3Rcyi0hRCnFXqBDNnCuGKFH6PdNmCC6ELc4eXy-zckaVyGHdcZ1ncEbFyAWnKJLW4hQcFgqNnHEwZgvOMRxdW2YoYRSTXSAa8Iup7uIt2aa8BgKmAINxMvUnHatOKa6e5slB5fqS3TgzVTg1eN5gn5cfvx-8Wlz_fXq88X59cZ1XJBNL1gvpAVDoCedl6PsvWfE804wq2TPpbJG9pR6KkDybmh_znrorTejNLY7Qad775LTrxVK1XMoDqbJREhr0XRQnRBMkK6hb_9Bb9OaY9tOU9VxKgmjvFHv9lSLW0oGr1ug2eR7TYnetaKXonetNPLNo2-1M4xP3N8aGvB-D9y1Nu7_59Hfbv7oHgCFQZXt</recordid><startdate>201710</startdate><enddate>201710</enddate><creator>Alburquerque, Nuria</creator><creator>Faize, Lydia</creator><creator>Burgos, Lorenzo</creator><general>John Wiley & Sons, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2399-8232</orcidid><orcidid>https://orcid.org/0000-0002-7770-5100</orcidid></search><sort><creationdate>201710</creationdate><title>Silencing of Agrobacterium tumefaciens oncogenes ipt and iaaM induces resistance to crown gall disease in plum but not in apricot</title><author>Alburquerque, Nuria ; Faize, Lydia ; Burgos, Lorenzo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3450-752756bea0e703f6d67ff20f4352b967469ba6711f15e643820fcbfe7bfad6ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Agrobacterium tumefaciens</topic><topic>Agrobacterium tumefaciens - genetics</topic><topic>apricot</topic><topic>Apricots</topic><topic>bacterial plant disease</topic><topic>Bacterial Proteins - genetics</topic><topic>Canker</topic><topic>Crown gall</topic><topic>Cultivars</topic><topic>Disease Resistance</topic><topic>Gall</topic><topic>Gene expression</topic><topic>Gene Silencing</topic><topic>Genetic modification</topic><topic>Greenhouses</topic><topic>In vitro methods and tests</topic><topic>Infections</topic><topic>oncogene silencing</topic><topic>Oncogenes</topic><topic>Oncogenes - genetics</topic><topic>Plant bacterial diseases</topic><topic>Plant Tumors - microbiology</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - microbiology</topic><topic>plum</topic><topic>Prunus</topic><topic>Prunus armeniaca - genetics</topic><topic>Prunus armeniaca - microbiology</topic><topic>Prunus domestica - genetics</topic><topic>Prunus domestica - microbiology</topic><topic>Reduction</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA-mediated interference</topic><topic>Rootstocks</topic><topic>siRNA</topic><topic>Transgenes</topic><topic>Transgenic plants</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alburquerque, Nuria</creatorcontrib><creatorcontrib>Faize, Lydia</creatorcontrib><creatorcontrib>Burgos, Lorenzo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Pest management science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alburquerque, Nuria</au><au>Faize, Lydia</au><au>Burgos, Lorenzo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Silencing of Agrobacterium tumefaciens oncogenes ipt and iaaM induces resistance to crown gall disease in plum but not in apricot</atitle><jtitle>Pest management science</jtitle><addtitle>Pest Manag Sci</addtitle><date>2017-10</date><risdate>2017</risdate><volume>73</volume><issue>10</issue><spage>2163</spage><epage>2173</epage><pages>2163-2173</pages><issn>1526-498X</issn><eissn>1526-4998</eissn><abstract>BACKGROUND
In this study, two vectors with short‐length chimeric transgenes were used to produce Prunus rootstocks resistant to crown gall disease through RNA‐interference‐mediated gene silencing of the Agrobacterium tumefaciens oncogenes ipt and iaaM.
RESULTS
Transgenic plum and apricot lines were produced with efficiencies of up to 7.7 and 1.1% respectively. An in vitro evaluation method allowed identification of susceptible lines and reduction in the number of lines to be evaluated in the greenhouse. Five transgenic plum lines, expressing transgene‐derived small interfering RNA (siRNA) and low levels of transgene hairpin RNA (hpRNA), showed a significant reduction in the development of the disease after infection with Agrobacterium strains C58 and A281 under greenhouse conditions. However, unexpectedly, all transgenic apricot lines were gall susceptible. The infection of apricot plants with a binary vector containing only the 6b oncogene demonstrated that the expression of this gene is involved in the induction of tumours in the apricot species.
CONCLUSION
RNAi‐mediated gene silencing can be used for inducing crown gall resistance in plum rootstocks. These could be used to graft non‐genetically modified commercial fruit cultivars reducing, or eliminating, the disease symptoms. © 2017 Society of Chemical Industry</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>28449201</pmid><doi>10.1002/ps.4600</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-2399-8232</orcidid><orcidid>https://orcid.org/0000-0002-7770-5100</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1526-498X |
| ispartof | Pest management science, 2017-10, Vol.73 (10), p.2163-2173 |
| issn | 1526-498X 1526-4998 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1893552503 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Agrobacterium tumefaciens Agrobacterium tumefaciens - genetics apricot Apricots bacterial plant disease Bacterial Proteins - genetics Canker Crown gall Cultivars Disease Resistance Gall Gene expression Gene Silencing Genetic modification Greenhouses In vitro methods and tests Infections oncogene silencing Oncogenes Oncogenes - genetics Plant bacterial diseases Plant Tumors - microbiology Plants, Genetically Modified - genetics Plants, Genetically Modified - microbiology plum Prunus Prunus armeniaca - genetics Prunus armeniaca - microbiology Prunus domestica - genetics Prunus domestica - microbiology Reduction Ribonucleic acid RNA RNA-mediated interference Rootstocks siRNA Transgenes Transgenic plants Tumors |
| title | Silencing of Agrobacterium tumefaciens oncogenes ipt and iaaM induces resistance to crown gall disease in plum but not in apricot |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T13%3A09%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Silencing%20of%20Agrobacterium%20tumefaciens%20oncogenes%20ipt%20and%20iaaM%20induces%20resistance%20to%20crown%20gall%20disease%20in%20plum%20but%20not%20in%20apricot&rft.jtitle=Pest%20management%20science&rft.au=Alburquerque,%20Nuria&rft.date=2017-10&rft.volume=73&rft.issue=10&rft.spage=2163&rft.epage=2173&rft.pages=2163-2173&rft.issn=1526-498X&rft.eissn=1526-4998&rft_id=info:doi/10.1002/ps.4600&rft_dat=%3Cproquest_cross%3E1893552503%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1934160214&rft_id=info:pmid/28449201&rfr_iscdi=true |