Differentially methylated Embryonal Fyn-associated Substrate (EFS) gene as a blood-specific epigenetic marker and its potential application in forensic casework

Highlights • A novel blood-specific differentially methylated locus (EFS) was investigated. • Most CpGs, especially CpG4, was found highly methylated in whole blood. • EFS methylation has the potential to differentiate between whole and menstrual blood. • The designed bisulfite pyrosequencing assay...

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Veröffentlicht in:	Forensic science international : genetics 2017-07, Vol.29, p.165-173
Hauptverfasser:	Vidaki, Athina, Johansson, Cecilia, Giangasparo, Federica
Format:	Artikel
Sprache:	eng
Schlagworte:	Adaptor Proteins, Signal Transducing - blood Adaptor Proteins, Signal Transducing - genetics Bisulfite pyrosequencing CpG Islands - genetics DNA Methylation EFS gene Epigenetics Epigenomics Forensic Genetics Forensic tissue identification blood Genetic Markers Humans Pathology Phosphoproteins - blood Phosphoproteins - genetics Sequence Analysis, DNA - methods
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container_title	Forensic science international : genetics
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creator	Vidaki, Athina Johansson, Cecilia Giangasparo, Federica
description	Highlights • A novel blood-specific differentially methylated locus (EFS) was investigated. • Most CpGs, especially CpG4, was found highly methylated in whole blood. • EFS methylation has the potential to differentiate between whole and menstrual blood. • The designed bisulfite pyrosequencing assay is both accurate and reproducible. • The method’s applicability to casework was demonstrated via aged and mixed stains.
doi_str_mv	10.1016/j.fsigen.2017.04.010
format	Article
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subjects	Adaptor Proteins, Signal Transducing - blood Adaptor Proteins, Signal Transducing - genetics Bisulfite pyrosequencing CpG Islands - genetics DNA Methylation EFS gene Epigenetics Epigenomics Forensic Genetics Forensic tissue identification blood Genetic Markers Humans Pathology Phosphoproteins - blood Phosphoproteins - genetics Sequence Analysis, DNA - methods
title	Differentially methylated Embryonal Fyn-associated Substrate (EFS) gene as a blood-specific epigenetic marker and its potential application in forensic casework
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