Fetal DNA does not induce preeclampsia-like symptoms when delivered in late pregnancy in the mouse
Abstract Introduction The etiology of preeclampsia is unclear. Fetal DNA is present in higher concentrations in the plasma of pregnant women suffering from preeclampsia than in the plasma of healthy pregnant women. A previously published study has shown that human fetal DNA injected into pregnant mi...
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| Veröffentlicht in: | Placenta (Eastbourne) 2017-04, Vol.52, p.100-105 |
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| creator | Čonka, Jozef Konečná, Barbora Lauková, Lucia Vlková, Barbora Celec, Peter |
| description | Abstract Introduction The etiology of preeclampsia is unclear. Fetal DNA is present in higher concentrations in the plasma of pregnant women suffering from preeclampsia than in the plasma of healthy pregnant women. A previously published study has shown that human fetal DNA injected into pregnant mice induces preeclampsia-like symptoms when administered between gestation days 10–14. The aim of our experiment was to determine whether or not similar effects would be induced by administration of human and mouse fetal DNA, as well as mouse adult DNA and lipopolysaccharide during late pregnancy in the mouse. Methods Experimental animals were injected daily intraperitoneally during gestation days 14–18 with either saline – negative control, lipopolysaccharide – positive control, or various types of DNA. On gestation day 19, blood pressure and proteinuria were measured, and placental and fetal weights were recorded. Results Fetal and placental hypotrophy were induced only by lipopolysaccharide (p |
| doi_str_mv | 10.1016/j.placenta.2017.02.008 |
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Fetal DNA is present in higher concentrations in the plasma of pregnant women suffering from preeclampsia than in the plasma of healthy pregnant women. A previously published study has shown that human fetal DNA injected into pregnant mice induces preeclampsia-like symptoms when administered between gestation days 10–14. The aim of our experiment was to determine whether or not similar effects would be induced by administration of human and mouse fetal DNA, as well as mouse adult DNA and lipopolysaccharide during late pregnancy in the mouse. Methods Experimental animals were injected daily intraperitoneally during gestation days 14–18 with either saline – negative control, lipopolysaccharide – positive control, or various types of DNA. On gestation day 19, blood pressure and proteinuria were measured, and placental and fetal weights were recorded. Results Fetal and placental hypotrophy were induced only by lipopolysaccharide (p < 0.001). Neither fetal nor adult DNA induced changes in fetal/placental weight. None of the experimental groups had higher blood pressure or urinary protein in comparison to saline treated animals. Discussion In our experiment, we found that there was no effect from intraperitoneally injected human fetal DNA, mouse fetal DNA, or mouse adult DNA on pregnant mice. Additionally, relatively high doses of various types of DNA did not induce preeclampsia-like symptoms in mice when administered in late pregnancy. Our negative results support the hypothesis that the increase of fetal DNA circulating in maternal circulation during the third trimester is rather a consequence than a cause of preeclampsia.</description><identifier>ISSN: 0143-4004</identifier><identifier>EISSN: 1532-3102</identifier><identifier>DOI: 10.1016/j.placenta.2017.02.008</identifier><identifier>PMID: 28454691</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Animals ; Blood Pressure - physiology ; Cell-free DNA ; Cell-Free Nucleic Acids - administration & dosage ; Disease Models, Animal ; Female ; Fetal hypotrophy ; Fetal nucleic acid ; Internal Medicine ; Lipopolysaccharides ; Mice ; Obstetrics and Gynecology ; Placenta - physiopathology ; Pre-Eclampsia - etiology ; Pre-Eclampsia - physiopathology ; Preeclampsia ; Pregnancy ; Pregnancy complication</subject><ispartof>Placenta (Eastbourne), 2017-04, Vol.52, p.100-105</ispartof><rights>2017 Elsevier Ltd</rights><rights>Copyright © 2017 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-ade10569effbec23b8ff992df0700f6ce105c206079e634e8869d3260112cded3</citedby><cites>FETCH-LOGICAL-c423t-ade10569effbec23b8ff992df0700f6ce105c206079e634e8869d3260112cded3</cites><orcidid>0000-0001-5883-3580</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.placenta.2017.02.008$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28454691$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Čonka, Jozef</creatorcontrib><creatorcontrib>Konečná, Barbora</creatorcontrib><creatorcontrib>Lauková, Lucia</creatorcontrib><creatorcontrib>Vlková, Barbora</creatorcontrib><creatorcontrib>Celec, Peter</creatorcontrib><title>Fetal DNA does not induce preeclampsia-like symptoms when delivered in late pregnancy in the mouse</title><title>Placenta (Eastbourne)</title><addtitle>Placenta</addtitle><description>Abstract Introduction The etiology of preeclampsia is unclear. Fetal DNA is present in higher concentrations in the plasma of pregnant women suffering from preeclampsia than in the plasma of healthy pregnant women. A previously published study has shown that human fetal DNA injected into pregnant mice induces preeclampsia-like symptoms when administered between gestation days 10–14. The aim of our experiment was to determine whether or not similar effects would be induced by administration of human and mouse fetal DNA, as well as mouse adult DNA and lipopolysaccharide during late pregnancy in the mouse. Methods Experimental animals were injected daily intraperitoneally during gestation days 14–18 with either saline – negative control, lipopolysaccharide – positive control, or various types of DNA. On gestation day 19, blood pressure and proteinuria were measured, and placental and fetal weights were recorded. Results Fetal and placental hypotrophy were induced only by lipopolysaccharide (p < 0.001). Neither fetal nor adult DNA induced changes in fetal/placental weight. None of the experimental groups had higher blood pressure or urinary protein in comparison to saline treated animals. Discussion In our experiment, we found that there was no effect from intraperitoneally injected human fetal DNA, mouse fetal DNA, or mouse adult DNA on pregnant mice. Additionally, relatively high doses of various types of DNA did not induce preeclampsia-like symptoms in mice when administered in late pregnancy. Our negative results support the hypothesis that the increase of fetal DNA circulating in maternal circulation during the third trimester is rather a consequence than a cause of preeclampsia.</description><subject>Animals</subject><subject>Blood Pressure - physiology</subject><subject>Cell-free DNA</subject><subject>Cell-Free Nucleic Acids - administration & dosage</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Fetal hypotrophy</subject><subject>Fetal nucleic acid</subject><subject>Internal Medicine</subject><subject>Lipopolysaccharides</subject><subject>Mice</subject><subject>Obstetrics and Gynecology</subject><subject>Placenta - physiopathology</subject><subject>Pre-Eclampsia - etiology</subject><subject>Pre-Eclampsia - physiopathology</subject><subject>Preeclampsia</subject><subject>Pregnancy</subject><subject>Pregnancy complication</subject><issn>0143-4004</issn><issn>1532-3102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EotvCK1Q-ckk6YyfZ5IKoWgpIFRyAs-W1J9Rbxwl2UrRvj8O2HLhwsmT_38z4G8bOEUoEbC725eS1oTDrUgBuSxAlQPuMbbCWopAI4jnbAFayqACqE3aa0h4AugrFS3Yi2qqumg43bHdDs_b8-vMltyMlHsaZu2AXQ3yKRMbrYUpOF97dE0-HYZrHIfFfdxS4Je8eKJLNAPd6_kP8CDqYw3oz3xEfxiXRK_ai1z7R68fzjH2_ef_t6mNx--XDp6vL28JUQs6FtoRQNx31_Y6MkLu277tO2B62AH1j1lcjoIFtR42sqG2bzkrRAKIwlqw8Y2-Odac4_lwozWpwyZD3OlCeQ2HbybpGiSJHm2PUxDGlSL2aoht0PCgEtfpVe_XkV61-FQiV_Wbw_LHHshvI_sWehObAu2OA8k8fHEWVjKNgyLpIZlZ2dP_v8fafEsa74Iz293SgtB-XGLJHhSplQH1dt7wuGbcSsEaQvwEjKKSK</recordid><startdate>20170401</startdate><enddate>20170401</enddate><creator>Čonka, Jozef</creator><creator>Konečná, Barbora</creator><creator>Lauková, Lucia</creator><creator>Vlková, Barbora</creator><creator>Celec, Peter</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5883-3580</orcidid></search><sort><creationdate>20170401</creationdate><title>Fetal DNA does not induce preeclampsia-like symptoms when delivered in late pregnancy in the mouse</title><author>Čonka, Jozef ; Konečná, Barbora ; Lauková, Lucia ; Vlková, Barbora ; Celec, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-ade10569effbec23b8ff992df0700f6ce105c206079e634e8869d3260112cded3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Blood Pressure - physiology</topic><topic>Cell-free DNA</topic><topic>Cell-Free Nucleic Acids - administration & dosage</topic><topic>Disease Models, Animal</topic><topic>Female</topic><topic>Fetal hypotrophy</topic><topic>Fetal nucleic acid</topic><topic>Internal Medicine</topic><topic>Lipopolysaccharides</topic><topic>Mice</topic><topic>Obstetrics and Gynecology</topic><topic>Placenta - physiopathology</topic><topic>Pre-Eclampsia - etiology</topic><topic>Pre-Eclampsia - physiopathology</topic><topic>Preeclampsia</topic><topic>Pregnancy</topic><topic>Pregnancy complication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Čonka, Jozef</creatorcontrib><creatorcontrib>Konečná, Barbora</creatorcontrib><creatorcontrib>Lauková, Lucia</creatorcontrib><creatorcontrib>Vlková, Barbora</creatorcontrib><creatorcontrib>Celec, Peter</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Placenta (Eastbourne)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Čonka, Jozef</au><au>Konečná, Barbora</au><au>Lauková, Lucia</au><au>Vlková, Barbora</au><au>Celec, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fetal DNA does not induce preeclampsia-like symptoms when delivered in late pregnancy in the mouse</atitle><jtitle>Placenta (Eastbourne)</jtitle><addtitle>Placenta</addtitle><date>2017-04-01</date><risdate>2017</risdate><volume>52</volume><spage>100</spage><epage>105</epage><pages>100-105</pages><issn>0143-4004</issn><eissn>1532-3102</eissn><abstract>Abstract Introduction The etiology of preeclampsia is unclear. Fetal DNA is present in higher concentrations in the plasma of pregnant women suffering from preeclampsia than in the plasma of healthy pregnant women. A previously published study has shown that human fetal DNA injected into pregnant mice induces preeclampsia-like symptoms when administered between gestation days 10–14. The aim of our experiment was to determine whether or not similar effects would be induced by administration of human and mouse fetal DNA, as well as mouse adult DNA and lipopolysaccharide during late pregnancy in the mouse. Methods Experimental animals were injected daily intraperitoneally during gestation days 14–18 with either saline – negative control, lipopolysaccharide – positive control, or various types of DNA. On gestation day 19, blood pressure and proteinuria were measured, and placental and fetal weights were recorded. Results Fetal and placental hypotrophy were induced only by lipopolysaccharide (p < 0.001). Neither fetal nor adult DNA induced changes in fetal/placental weight. None of the experimental groups had higher blood pressure or urinary protein in comparison to saline treated animals. Discussion In our experiment, we found that there was no effect from intraperitoneally injected human fetal DNA, mouse fetal DNA, or mouse adult DNA on pregnant mice. Additionally, relatively high doses of various types of DNA did not induce preeclampsia-like symptoms in mice when administered in late pregnancy. Our negative results support the hypothesis that the increase of fetal DNA circulating in maternal circulation during the third trimester is rather a consequence than a cause of preeclampsia.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>28454691</pmid><doi>10.1016/j.placenta.2017.02.008</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0001-5883-3580</orcidid></addata></record> |
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| subjects | Animals Blood Pressure - physiology Cell-free DNA Cell-Free Nucleic Acids - administration & dosage Disease Models, Animal Female Fetal hypotrophy Fetal nucleic acid Internal Medicine Lipopolysaccharides Mice Obstetrics and Gynecology Placenta - physiopathology Pre-Eclampsia - etiology Pre-Eclampsia - physiopathology Preeclampsia Pregnancy Pregnancy complication |
| title | Fetal DNA does not induce preeclampsia-like symptoms when delivered in late pregnancy in the mouse |
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