Aggregation and Adhesion Activity of Lactobacilli Isolated from Fermented Products In Vitro and In Vivo: a Potential Probiotic Strain
Approximately 25 strains of lactobacilli isolated from different dairy products and fermented vegetables were screened according to their possibility to show the high auto-aggregation and co-aggregation. The strains Lactobacillus helveticus INRA-2010-H11, Lactobacillus rhamnosus INA-5.1, and Lactoba...
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| Veröffentlicht in: | Probiotics and antimicrobial proteins 2018-06, Vol.10 (2), p.269-276 |
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| creator | Grigoryan, S. Bazukyan, I. Trchounian, A. |
| description | Approximately 25 strains of lactobacilli isolated from different dairy products and fermented vegetables were screened according to their possibility to show the high auto-aggregation and co-aggregation. The strains
Lactobacillus helveticus
INRA-2010-H11,
Lactobacillus rhamnosus
INA-5.1, and
Lactobacillus acidophilus
JM-2012 were determined to have the high auto-aggregation (approximately 73, 46, and 70.5% correspondingly). A high co-aggregation capacity (75.53%) for strains INRA-2010-H11 and JM-2012 was shown. The adhesion degree of INRA-2010-H11 on the surface of buccal epithelial cells was 88.23%. The study of INRA-2010-H11, JM-2012, and both strains’ mixture (1:1) adhesion capacity on the surface of epithelial HeLa cells revealed the adhesion of 1.1 × 10
6
, 6.3 × 10
4
, and 2.3 × 10
5
CFU, respectively, from starter amount of CFU 10
7
and 10
8
for both strains. In vivo experiments of LAB adhesion in gastrointestinal tract of mouse revealed the presence of 2.5 × 10
9
, 1.2 × 10
9
, and 1.5 × 10
9
CFU of LAB in control and groups of mouse, fed by INRA-2010-H11 and mixture, respectively. Feeding by investigated lactobacilli was suggested to lead to microbiota biodiversity reduction in small intestine and colon and its augmentation in stomach. Thus, INRA-2010-H11 demonstrated a high aggregation and adhesion activity so it has the potential as a good probiotic strain. |
| doi_str_mv | 10.1007/s12602-017-9283-9 |
| format | Article |
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Lactobacillus helveticus
INRA-2010-H11,
Lactobacillus rhamnosus
INA-5.1, and
Lactobacillus acidophilus
JM-2012 were determined to have the high auto-aggregation (approximately 73, 46, and 70.5% correspondingly). A high co-aggregation capacity (75.53%) for strains INRA-2010-H11 and JM-2012 was shown. The adhesion degree of INRA-2010-H11 on the surface of buccal epithelial cells was 88.23%. The study of INRA-2010-H11, JM-2012, and both strains’ mixture (1:1) adhesion capacity on the surface of epithelial HeLa cells revealed the adhesion of 1.1 × 10
6
, 6.3 × 10
4
, and 2.3 × 10
5
CFU, respectively, from starter amount of CFU 10
7
and 10
8
for both strains. In vivo experiments of LAB adhesion in gastrointestinal tract of mouse revealed the presence of 2.5 × 10
9
, 1.2 × 10
9
, and 1.5 × 10
9
CFU of LAB in control and groups of mouse, fed by INRA-2010-H11 and mixture, respectively. Feeding by investigated lactobacilli was suggested to lead to microbiota biodiversity reduction in small intestine and colon and its augmentation in stomach. Thus, INRA-2010-H11 demonstrated a high aggregation and adhesion activity so it has the potential as a good probiotic strain.</description><identifier>ISSN: 1867-1306</identifier><identifier>EISSN: 1867-1314</identifier><identifier>DOI: 10.1007/s12602-017-9283-9</identifier><identifier>PMID: 28455689</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adolescent ; Adult ; Animals ; Applied Microbiology ; Bacterial Adhesion ; Biodiversity ; Cheek - microbiology ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Dairy Products - microbiology ; Epithelial Cells - microbiology ; Feces - microbiology ; Female ; Fermented Foods - microbiology ; Gastrointestinal Microbiome ; Gastrointestinal Tract - microbiology ; HeLa Cells ; Humans ; Lactobacillus - classification ; Lactobacillus - genetics ; Lactobacillus - isolation & purification ; Lactobacillus - physiology ; Mice ; Mice, Inbred BALB C ; Microbiology ; Nutrition ; Probiotics - chemistry ; Probiotics - classification ; Probiotics - isolation & purification ; Protein Science ; Young Adult</subject><ispartof>Probiotics and antimicrobial proteins, 2018-06, Vol.10 (2), p.269-276</ispartof><rights>Springer Science+Business Media New York 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c344t-6daea75cc87508a515fd21f6a6cbfa66d8ec49918d4791cdc1b34fbfd07f656e3</citedby><cites>FETCH-LOGICAL-c344t-6daea75cc87508a515fd21f6a6cbfa66d8ec49918d4791cdc1b34fbfd07f656e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12602-017-9283-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12602-017-9283-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28455689$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Grigoryan, S.</creatorcontrib><creatorcontrib>Bazukyan, I.</creatorcontrib><creatorcontrib>Trchounian, A.</creatorcontrib><title>Aggregation and Adhesion Activity of Lactobacilli Isolated from Fermented Products In Vitro and In Vivo: a Potential Probiotic Strain</title><title>Probiotics and antimicrobial proteins</title><addtitle>Probiotics & Antimicro. Prot</addtitle><addtitle>Probiotics Antimicrob Proteins</addtitle><description>Approximately 25 strains of lactobacilli isolated from different dairy products and fermented vegetables were screened according to their possibility to show the high auto-aggregation and co-aggregation. The strains
Lactobacillus helveticus
INRA-2010-H11,
Lactobacillus rhamnosus
INA-5.1, and
Lactobacillus acidophilus
JM-2012 were determined to have the high auto-aggregation (approximately 73, 46, and 70.5% correspondingly). A high co-aggregation capacity (75.53%) for strains INRA-2010-H11 and JM-2012 was shown. The adhesion degree of INRA-2010-H11 on the surface of buccal epithelial cells was 88.23%. The study of INRA-2010-H11, JM-2012, and both strains’ mixture (1:1) adhesion capacity on the surface of epithelial HeLa cells revealed the adhesion of 1.1 × 10
6
, 6.3 × 10
4
, and 2.3 × 10
5
CFU, respectively, from starter amount of CFU 10
7
and 10
8
for both strains. In vivo experiments of LAB adhesion in gastrointestinal tract of mouse revealed the presence of 2.5 × 10
9
, 1.2 × 10
9
, and 1.5 × 10
9
CFU of LAB in control and groups of mouse, fed by INRA-2010-H11 and mixture, respectively. Feeding by investigated lactobacilli was suggested to lead to microbiota biodiversity reduction in small intestine and colon and its augmentation in stomach. Thus, INRA-2010-H11 demonstrated a high aggregation and adhesion activity so it has the potential as a good probiotic strain.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Animals</subject><subject>Applied Microbiology</subject><subject>Bacterial Adhesion</subject><subject>Biodiversity</subject><subject>Cheek - microbiology</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>Dairy Products - microbiology</subject><subject>Epithelial Cells - microbiology</subject><subject>Feces - microbiology</subject><subject>Female</subject><subject>Fermented Foods - microbiology</subject><subject>Gastrointestinal Microbiome</subject><subject>Gastrointestinal Tract - microbiology</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Lactobacillus - classification</subject><subject>Lactobacillus - genetics</subject><subject>Lactobacillus - isolation & purification</subject><subject>Lactobacillus - physiology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Nutrition</subject><subject>Probiotics - chemistry</subject><subject>Probiotics - classification</subject><subject>Probiotics - isolation & purification</subject><subject>Protein Science</subject><subject>Young Adult</subject><issn>1867-1306</issn><issn>1867-1314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKAzEUhoMotlYfwI1k6WY0mUsmcVfES6Fgwcs2ZHKpKTOTmmSEPoDv7Uxbu3R1zk--80M-AC4xusEIlbcBpwSlCcJlwlKaJewIjDElZYIznB8fdkRG4CyEFUKEZCk6BaOU5kVBKBuDn-ly6fVSROtaKFoFp-pThyFMZbTfNm6gM3AuZHSVkLauLZwFV4uoFTTeNfBR-0a3Q1x4pzoZA5y18MNG77Z92_Dt7qCACxd70op6QCvropXwNXph23NwYkQd9MV-TsD748Pb_XMyf3ma3U_niczyPCZECS3KQkpaFoiKAhdGpdgQQWRlBCGKapkzhqnKS4alkrjKclMZhUpDCqKzCbje9a69--p0iLyxQeq6Fq12XeCYsqzIGU1xj-IdKr0LwWvD1942wm84Rnywz3f2eW-fD_Y562-u9vVd1Wh1uPjT3QPpDgj9U7vUnq9c59v-y_-0_gKT9ZHT</recordid><startdate>20180601</startdate><enddate>20180601</enddate><creator>Grigoryan, S.</creator><creator>Bazukyan, I.</creator><creator>Trchounian, A.</creator><general>Springer US</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20180601</creationdate><title>Aggregation and Adhesion Activity of Lactobacilli Isolated from Fermented Products In Vitro and In Vivo: a Potential Probiotic Strain</title><author>Grigoryan, S. ; Bazukyan, I. ; Trchounian, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-6daea75cc87508a515fd21f6a6cbfa66d8ec49918d4791cdc1b34fbfd07f656e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Animals</topic><topic>Applied Microbiology</topic><topic>Bacterial Adhesion</topic><topic>Biodiversity</topic><topic>Cheek - microbiology</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemistry/Food Science</topic><topic>Dairy Products - microbiology</topic><topic>Epithelial Cells - microbiology</topic><topic>Feces - microbiology</topic><topic>Female</topic><topic>Fermented Foods - microbiology</topic><topic>Gastrointestinal Microbiome</topic><topic>Gastrointestinal Tract - microbiology</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Lactobacillus - classification</topic><topic>Lactobacillus - genetics</topic><topic>Lactobacillus - isolation & purification</topic><topic>Lactobacillus - physiology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Nutrition</topic><topic>Probiotics - chemistry</topic><topic>Probiotics - classification</topic><topic>Probiotics - isolation & purification</topic><topic>Protein Science</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grigoryan, S.</creatorcontrib><creatorcontrib>Bazukyan, I.</creatorcontrib><creatorcontrib>Trchounian, A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Probiotics and antimicrobial proteins</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grigoryan, S.</au><au>Bazukyan, I.</au><au>Trchounian, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aggregation and Adhesion Activity of Lactobacilli Isolated from Fermented Products In Vitro and In Vivo: a Potential Probiotic Strain</atitle><jtitle>Probiotics and antimicrobial proteins</jtitle><stitle>Probiotics & Antimicro. Prot</stitle><addtitle>Probiotics Antimicrob Proteins</addtitle><date>2018-06-01</date><risdate>2018</risdate><volume>10</volume><issue>2</issue><spage>269</spage><epage>276</epage><pages>269-276</pages><issn>1867-1306</issn><eissn>1867-1314</eissn><abstract>Approximately 25 strains of lactobacilli isolated from different dairy products and fermented vegetables were screened according to their possibility to show the high auto-aggregation and co-aggregation. The strains
Lactobacillus helveticus
INRA-2010-H11,
Lactobacillus rhamnosus
INA-5.1, and
Lactobacillus acidophilus
JM-2012 were determined to have the high auto-aggregation (approximately 73, 46, and 70.5% correspondingly). A high co-aggregation capacity (75.53%) for strains INRA-2010-H11 and JM-2012 was shown. The adhesion degree of INRA-2010-H11 on the surface of buccal epithelial cells was 88.23%. The study of INRA-2010-H11, JM-2012, and both strains’ mixture (1:1) adhesion capacity on the surface of epithelial HeLa cells revealed the adhesion of 1.1 × 10
6
, 6.3 × 10
4
, and 2.3 × 10
5
CFU, respectively, from starter amount of CFU 10
7
and 10
8
for both strains. In vivo experiments of LAB adhesion in gastrointestinal tract of mouse revealed the presence of 2.5 × 10
9
, 1.2 × 10
9
, and 1.5 × 10
9
CFU of LAB in control and groups of mouse, fed by INRA-2010-H11 and mixture, respectively. Feeding by investigated lactobacilli was suggested to lead to microbiota biodiversity reduction in small intestine and colon and its augmentation in stomach. Thus, INRA-2010-H11 demonstrated a high aggregation and adhesion activity so it has the potential as a good probiotic strain.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>28455689</pmid><doi>10.1007/s12602-017-9283-9</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Adolescent Adult Animals Applied Microbiology Bacterial Adhesion Biodiversity Cheek - microbiology Chemistry Chemistry and Materials Science Chemistry/Food Science Dairy Products - microbiology Epithelial Cells - microbiology Feces - microbiology Female Fermented Foods - microbiology Gastrointestinal Microbiome Gastrointestinal Tract - microbiology HeLa Cells Humans Lactobacillus - classification Lactobacillus - genetics Lactobacillus - isolation & purification Lactobacillus - physiology Mice Mice, Inbred BALB C Microbiology Nutrition Probiotics - chemistry Probiotics - classification Probiotics - isolation & purification Protein Science Young Adult |
| title | Aggregation and Adhesion Activity of Lactobacilli Isolated from Fermented Products In Vitro and In Vivo: a Potential Probiotic Strain |
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