Pentoxifylline increase sperm motility in devitrified spermatozoa from asthenozoospermic patient without damage chromatin and DNA integrity
The freeze–thaw process results in reduced motility, viability and fertilization potential of human spermatozoa. So, a variety of substances were evaluated in order to enhance human sperm resistance to the stress of cryopreservation, such as Pentoxifylline (PTX) for improving the Intracytoplasmic sp...
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| Veröffentlicht in: | Cryobiology 2017-06, Vol.76, p.59-64 |
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| creator | Nabi, Ali Khalili, Mohammad Ali Fesahat, Farzaneh Talebi, Alireza Ghasemi-Esmailabad, Saeed |
| description | The freeze–thaw process results in reduced motility, viability and fertilization potential of human spermatozoa. So, a variety of substances were evaluated in order to enhance human sperm resistance to the stress of cryopreservation, such as Pentoxifylline (PTX) for improving the Intracytoplasmic sperm injection (ICSI) outcomes. The aim was to investigate the effect of PTX on sperm parameters and chromatin/DNA integrity of asthenozoospermic semen post vitrification. A total of 30 semen specimens were obtained from infertile men with asthenozoospermia. The cryoprotectant-free vitrification was performed for the samples after assessment of sperm parameters. After warming, each sample was exposed for 30 min to 3.6 mmol/l PTX in experimental group and the control group without any treatment apposing at 37 °C for 30 min in regard, to repeat all in vitro analysis (sperm parameters and DNA integrity assay). Regardless of the vitrification devastating impacts on sperm parameters, incubation of post vitrified samples with PTX increased the rate of progressive motility (P |
| doi_str_mv | 10.1016/j.cryobiol.2017.04.008 |
| format | Article |
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So, a variety of substances were evaluated in order to enhance human sperm resistance to the stress of cryopreservation, such as Pentoxifylline (PTX) for improving the Intracytoplasmic sperm injection (ICSI) outcomes. The aim was to investigate the effect of PTX on sperm parameters and chromatin/DNA integrity of asthenozoospermic semen post vitrification. A total of 30 semen specimens were obtained from infertile men with asthenozoospermia. The cryoprotectant-free vitrification was performed for the samples after assessment of sperm parameters. After warming, each sample was exposed for 30 min to 3.6 mmol/l PTX in experimental group and the control group without any treatment apposing at 37 °C for 30 min in regard, to repeat all in vitro analysis (sperm parameters and DNA integrity assay). Regardless of the vitrification devastating impacts on sperm parameters, incubation of post vitrified samples with PTX increased the rate of progressive motility (P < 0.01). Moreover, PTX addition did not significantly damage DNA integrity of asthenozoospermic sperm samples. The data showed that PTX was able to improve sperm movement without any adverse effects on sperm chromatin/DNA integrity in vitrification program.</description><identifier>ISSN: 0011-2240</identifier><identifier>EISSN: 1090-2392</identifier><identifier>DOI: 10.1016/j.cryobiol.2017.04.008</identifier><identifier>PMID: 28455156</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Adult ; Asthenozoospermia ; Chromatin ; Cryopreservation ; DNA Damage ; DNA integrity ; Humans ; Male ; Middle Aged ; Pentoxifylline ; Pentoxifylline - pharmacology ; Semen Preservation ; Sperm Injections, Intracytoplasmic ; Sperm Motility - drug effects ; Spermatozoa ; Vitrification</subject><ispartof>Cryobiology, 2017-06, Vol.76, p.59-64</ispartof><rights>2017 Elsevier Inc.</rights><rights>Copyright © 2017 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-45b1ea8572f6d8e596d29eb793735291cf3b86f0d04df5c490d70182abc44dbc3</citedby><cites>FETCH-LOGICAL-c368t-45b1ea8572f6d8e596d29eb793735291cf3b86f0d04df5c490d70182abc44dbc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cryobiol.2017.04.008$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28455156$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nabi, Ali</creatorcontrib><creatorcontrib>Khalili, Mohammad Ali</creatorcontrib><creatorcontrib>Fesahat, Farzaneh</creatorcontrib><creatorcontrib>Talebi, Alireza</creatorcontrib><creatorcontrib>Ghasemi-Esmailabad, Saeed</creatorcontrib><title>Pentoxifylline increase sperm motility in devitrified spermatozoa from asthenozoospermic patient without damage chromatin and DNA integrity</title><title>Cryobiology</title><addtitle>Cryobiology</addtitle><description>The freeze–thaw process results in reduced motility, viability and fertilization potential of human spermatozoa. So, a variety of substances were evaluated in order to enhance human sperm resistance to the stress of cryopreservation, such as Pentoxifylline (PTX) for improving the Intracytoplasmic sperm injection (ICSI) outcomes. The aim was to investigate the effect of PTX on sperm parameters and chromatin/DNA integrity of asthenozoospermic semen post vitrification. A total of 30 semen specimens were obtained from infertile men with asthenozoospermia. The cryoprotectant-free vitrification was performed for the samples after assessment of sperm parameters. After warming, each sample was exposed for 30 min to 3.6 mmol/l PTX in experimental group and the control group without any treatment apposing at 37 °C for 30 min in regard, to repeat all in vitro analysis (sperm parameters and DNA integrity assay). Regardless of the vitrification devastating impacts on sperm parameters, incubation of post vitrified samples with PTX increased the rate of progressive motility (P < 0.01). Moreover, PTX addition did not significantly damage DNA integrity of asthenozoospermic sperm samples. The data showed that PTX was able to improve sperm movement without any adverse effects on sperm chromatin/DNA integrity in vitrification program.</description><subject>Adult</subject><subject>Asthenozoospermia</subject><subject>Chromatin</subject><subject>Cryopreservation</subject><subject>DNA Damage</subject><subject>DNA integrity</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Pentoxifylline</subject><subject>Pentoxifylline - pharmacology</subject><subject>Semen Preservation</subject><subject>Sperm Injections, Intracytoplasmic</subject><subject>Sperm Motility - drug effects</subject><subject>Spermatozoa</subject><subject>Vitrification</subject><issn>0011-2240</issn><issn>1090-2392</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2OFCEUhYnROO3oK0xYuqnyQkEV7JyMv8lEXeiaUHBrmk5V0QI92r6CLy1jz7h1ReB895xwDyEXDFoGrH-1a106xjHEueXAhhZEC6AekQ0DDQ3vNH9MNgCMNZwLOCPPct4BQD904ik540pIyWS_Ib-_4FrizzAd5zmsSMPqEtqMNO8xLXSJJcyhHOs79XgbSgpTQH9SbYm_oqVTigu1uWxxrff4VwqO7m0J1Zv-CGUbD4V6u9gbpG5b8Sqt1K6evvl0Wa0L3qQa8pw8meyc8cX9eU6-vXv79epDc_35_cery-vGdb0qjZAjQ6vkwKfeK5S691zjOOhu6CTXzE3dqPoJPAg_SSc0-AGY4nZ0QvjRdefk5cl3n-L3A-ZilpAdzrNdMR6yYUp3UmgxqIr2J9SlmHPCyexTWGw6GgbmrgizMw9FmLsiDAhTi6iDF_cZh3FB_2_sYfMVeH0CsP70NmAy2dV9OfQhoSvGx_C_jD-emqH-</recordid><startdate>201706</startdate><enddate>201706</enddate><creator>Nabi, Ali</creator><creator>Khalili, Mohammad Ali</creator><creator>Fesahat, Farzaneh</creator><creator>Talebi, Alireza</creator><creator>Ghasemi-Esmailabad, Saeed</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201706</creationdate><title>Pentoxifylline increase sperm motility in devitrified spermatozoa from asthenozoospermic patient without damage chromatin and DNA integrity</title><author>Nabi, Ali ; Khalili, Mohammad Ali ; Fesahat, Farzaneh ; Talebi, Alireza ; Ghasemi-Esmailabad, Saeed</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-45b1ea8572f6d8e596d29eb793735291cf3b86f0d04df5c490d70182abc44dbc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>Asthenozoospermia</topic><topic>Chromatin</topic><topic>Cryopreservation</topic><topic>DNA Damage</topic><topic>DNA integrity</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Pentoxifylline</topic><topic>Pentoxifylline - pharmacology</topic><topic>Semen Preservation</topic><topic>Sperm Injections, Intracytoplasmic</topic><topic>Sperm Motility - drug effects</topic><topic>Spermatozoa</topic><topic>Vitrification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nabi, Ali</creatorcontrib><creatorcontrib>Khalili, Mohammad Ali</creatorcontrib><creatorcontrib>Fesahat, Farzaneh</creatorcontrib><creatorcontrib>Talebi, Alireza</creatorcontrib><creatorcontrib>Ghasemi-Esmailabad, Saeed</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cryobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nabi, Ali</au><au>Khalili, Mohammad Ali</au><au>Fesahat, Farzaneh</au><au>Talebi, Alireza</au><au>Ghasemi-Esmailabad, Saeed</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pentoxifylline increase sperm motility in devitrified spermatozoa from asthenozoospermic patient without damage chromatin and DNA integrity</atitle><jtitle>Cryobiology</jtitle><addtitle>Cryobiology</addtitle><date>2017-06</date><risdate>2017</risdate><volume>76</volume><spage>59</spage><epage>64</epage><pages>59-64</pages><issn>0011-2240</issn><eissn>1090-2392</eissn><abstract>The freeze–thaw process results in reduced motility, viability and fertilization potential of human spermatozoa. 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| ispartof | Cryobiology, 2017-06, Vol.76, p.59-64 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adult Asthenozoospermia Chromatin Cryopreservation DNA Damage DNA integrity Humans Male Middle Aged Pentoxifylline Pentoxifylline - pharmacology Semen Preservation Sperm Injections, Intracytoplasmic Sperm Motility - drug effects Spermatozoa Vitrification |
| title | Pentoxifylline increase sperm motility in devitrified spermatozoa from asthenozoospermic patient without damage chromatin and DNA integrity |
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