High-content analysis screening for cell cycle regulators using arrayed synthetic crRNA libraries

•First use of arrayed, synthetic crRNAs in a multiparametric gene knockout screen.•High-content analysis was used to identify hits in a cell cycle reporter assay.•7 experimental parameters measured in live cells to classify cell into 6 categories.•Hit stratification: target gene expression, indel fo...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Journal of biotechnology 2017-06, Vol.251, p.189-200
Hauptverfasser:	Strezoska, Žaklina, Perkett, Matthew R., Chou, Eldon T., Maksimova, Elena, Anderson, Emily M., McClelland, Shawn, Kelley, Melissa L., Vermeulen, Annaleen, Smith, Anja van Brabant
Format:	Artikel
Sprache:	eng
Schlagworte:	Arrayed crRNA libraries Cell Cycle - genetics Cell cycle regulators Cell Line, Tumor CRISPR-Cas Systems CRISPR-Cas9 Gene editing High-content analysis screen High-Throughput Nucleotide Sequencing Humans Lentivirus - genetics Phenotype RNA - genetics Synthetic crRNA
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!