Non-neuronal neuropeptide Y and its receptors during acute rejection of rat pulmonary allografts
Abstract This study tested the hypothesis that neuropeptide Y (NPY) and NPY receptors 1 (Y1) and 2 (Y2) participate in lung allograft rejection. Inflammation in grafts may include interaction between blood leukocytes and graft endothelial cells and marked accumulation of intravascular blood leukocyt...
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| Veröffentlicht in: | Transplant immunology 2017-08, Vol.43-44, p.49-53 |
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| creator | Schmitz, Jessica Zakrzewicz, Anna Wilker, Sigrid Kuncová, Jitka Hecker, Andreas Grau, Veronika Padberg, Winfried Holler, Julia P.N |
| description | Abstract This study tested the hypothesis that neuropeptide Y (NPY) and NPY receptors 1 (Y1) and 2 (Y2) participate in lung allograft rejection. Inflammation in grafts may include interaction between blood leukocytes and graft endothelial cells and marked accumulation of intravascular blood leukocytes. Fewer leukocytes accumulate in lung than in kidney allografts. Lung transplantion was performed in the Dark Agouti to Lewis rat strain combination. Intravascular and intraalveolar leukocytes were isolated from the grafts, and we evaluated the mRNA expression of NPY, Y1, and Y2 by real-time RT-PCR as well as the peptide expression of NPY by radioimmunoassay and immunohistochemistry. NPY and Y1 were expressed by pulmonary intravascular and intraalveolar leukocytes. Y1 was up-regulated by pulmonary intravascular and intraalveolar leukocytes during allograft rejection while Y2 could not be detected. Higher NPY expression levels in intravascular leukocytes were observed in lung compared to kidney allografts, which were investigated previously. Our findings suggest that an increased leukocytic expression of NPY in lung compared to kidney allografts results in a reduced accumulation of leukocytes in allograft vessels. |
| doi_str_mv | 10.1016/j.trim.2017.04.004 |
| format | Article |
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Inflammation in grafts may include interaction between blood leukocytes and graft endothelial cells and marked accumulation of intravascular blood leukocytes. Fewer leukocytes accumulate in lung than in kidney allografts. Lung transplantion was performed in the Dark Agouti to Lewis rat strain combination. Intravascular and intraalveolar leukocytes were isolated from the grafts, and we evaluated the mRNA expression of NPY, Y1, and Y2 by real-time RT-PCR as well as the peptide expression of NPY by radioimmunoassay and immunohistochemistry. NPY and Y1 were expressed by pulmonary intravascular and intraalveolar leukocytes. Y1 was up-regulated by pulmonary intravascular and intraalveolar leukocytes during allograft rejection while Y2 could not be detected. Higher NPY expression levels in intravascular leukocytes were observed in lung compared to kidney allografts, which were investigated previously. Our findings suggest that an increased leukocytic expression of NPY in lung compared to kidney allografts results in a reduced accumulation of leukocytes in allograft vessels.</description><identifier>ISSN: 0966-3274</identifier><identifier>EISSN: 1878-5492</identifier><identifier>DOI: 10.1016/j.trim.2017.04.004</identifier><identifier>PMID: 28438668</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Acute Disease ; Allergy and Immunology ; Allografts ; Animals ; Gene Expression Regulation - immunology ; Graft Rejection - immunology ; Graft Rejection - pathology ; Immunosuppression ; Inflammation ; Leukocytes ; Leukocytes - immunology ; Leukocytes - pathology ; Lung - immunology ; Lung - pathology ; Lung Transplantation ; Neuropeptide Y ; Neuropeptide Y - immunology ; Rats ; Rats, Inbred Lew ; Receptors, G-Protein-Coupled - immunology ; Receptors, Neuropeptide - immunology ; Receptors, Neuropeptide Y - immunology ; Transplantion</subject><ispartof>Transplant immunology, 2017-08, Vol.43-44, p.49-53</ispartof><rights>2017</rights><rights>Copyright © 2017. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-c449980ab54e8ac0d749f2e44c179ab85ce1ba17171366edbd34d5acee14e7d53</citedby><cites>FETCH-LOGICAL-c411t-c449980ab54e8ac0d749f2e44c179ab85ce1ba17171366edbd34d5acee14e7d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0966327416301642$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28438668$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schmitz, Jessica</creatorcontrib><creatorcontrib>Zakrzewicz, Anna</creatorcontrib><creatorcontrib>Wilker, Sigrid</creatorcontrib><creatorcontrib>Kuncová, Jitka</creatorcontrib><creatorcontrib>Hecker, Andreas</creatorcontrib><creatorcontrib>Grau, Veronika</creatorcontrib><creatorcontrib>Padberg, Winfried</creatorcontrib><creatorcontrib>Holler, Julia P.N</creatorcontrib><title>Non-neuronal neuropeptide Y and its receptors during acute rejection of rat pulmonary allografts</title><title>Transplant immunology</title><addtitle>Transpl Immunol</addtitle><description>Abstract This study tested the hypothesis that neuropeptide Y (NPY) and NPY receptors 1 (Y1) and 2 (Y2) participate in lung allograft rejection. Inflammation in grafts may include interaction between blood leukocytes and graft endothelial cells and marked accumulation of intravascular blood leukocytes. Fewer leukocytes accumulate in lung than in kidney allografts. Lung transplantion was performed in the Dark Agouti to Lewis rat strain combination. Intravascular and intraalveolar leukocytes were isolated from the grafts, and we evaluated the mRNA expression of NPY, Y1, and Y2 by real-time RT-PCR as well as the peptide expression of NPY by radioimmunoassay and immunohistochemistry. NPY and Y1 were expressed by pulmonary intravascular and intraalveolar leukocytes. Y1 was up-regulated by pulmonary intravascular and intraalveolar leukocytes during allograft rejection while Y2 could not be detected. Higher NPY expression levels in intravascular leukocytes were observed in lung compared to kidney allografts, which were investigated previously. Our findings suggest that an increased leukocytic expression of NPY in lung compared to kidney allografts results in a reduced accumulation of leukocytes in allograft vessels.</description><subject>Acute Disease</subject><subject>Allergy and Immunology</subject><subject>Allografts</subject><subject>Animals</subject><subject>Gene Expression Regulation - immunology</subject><subject>Graft Rejection - immunology</subject><subject>Graft Rejection - pathology</subject><subject>Immunosuppression</subject><subject>Inflammation</subject><subject>Leukocytes</subject><subject>Leukocytes - immunology</subject><subject>Leukocytes - pathology</subject><subject>Lung - immunology</subject><subject>Lung - pathology</subject><subject>Lung Transplantation</subject><subject>Neuropeptide Y</subject><subject>Neuropeptide Y - immunology</subject><subject>Rats</subject><subject>Rats, Inbred Lew</subject><subject>Receptors, G-Protein-Coupled - immunology</subject><subject>Receptors, Neuropeptide - immunology</subject><subject>Receptors, Neuropeptide Y - immunology</subject><subject>Transplantion</subject><issn>0966-3274</issn><issn>1878-5492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUGP1SAUhYnROM_RP-DCsHTTCpQWmhgTM1Fnkoku1IUrpHA7ofKgAp1k_r3UN7pwYSBAbs454X4XoeeUtJTQ4dXSluSOLSNUtIS3hPAH6EClkE3PR_YQHcg4DE3HBD9DT3JeCCGsH8VjdMYk7-QwyAP6_jGGJsCWYtAe_36ssBZnAX_DOljsSsYJTK3FlLHdkgs3WJutQC0vYIqLAccZJ13wuvljzUl3WHsfb5KeS36KHs3aZ3h2f5-jr-_ffbm4bK4_fbi6eHvdGE5pqScfR0n01HOQ2hAr-Dgz4NxQMepJ9gbopKmoqxsGsJPtuO21AaAchO27c_TylLum-HODXNTRZQPe6wBxy4rKcd-UiSplJ6lJMecEs1orx_ptRYnayapF7WTVTlYRrirZanpxn79NR7B_LX9QVsHrkwBql7cOksrGQTBgXeVXlI3u__lv_rEb74Iz2v-AO8hL3FKdUO1DZaaI-rzPdh8tHbqayFn3C5RFoPQ</recordid><startdate>20170801</startdate><enddate>20170801</enddate><creator>Schmitz, Jessica</creator><creator>Zakrzewicz, Anna</creator><creator>Wilker, Sigrid</creator><creator>Kuncová, Jitka</creator><creator>Hecker, Andreas</creator><creator>Grau, Veronika</creator><creator>Padberg, Winfried</creator><creator>Holler, Julia P.N</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20170801</creationdate><title>Non-neuronal neuropeptide Y and its receptors during acute rejection of rat pulmonary allografts</title><author>Schmitz, Jessica ; Zakrzewicz, Anna ; Wilker, Sigrid ; Kuncová, Jitka ; Hecker, Andreas ; Grau, Veronika ; Padberg, Winfried ; Holler, Julia P.N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-c449980ab54e8ac0d749f2e44c179ab85ce1ba17171366edbd34d5acee14e7d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Acute Disease</topic><topic>Allergy and Immunology</topic><topic>Allografts</topic><topic>Animals</topic><topic>Gene Expression Regulation - immunology</topic><topic>Graft Rejection - immunology</topic><topic>Graft Rejection - pathology</topic><topic>Immunosuppression</topic><topic>Inflammation</topic><topic>Leukocytes</topic><topic>Leukocytes - immunology</topic><topic>Leukocytes - pathology</topic><topic>Lung - immunology</topic><topic>Lung - pathology</topic><topic>Lung Transplantation</topic><topic>Neuropeptide Y</topic><topic>Neuropeptide Y - immunology</topic><topic>Rats</topic><topic>Rats, Inbred Lew</topic><topic>Receptors, G-Protein-Coupled - immunology</topic><topic>Receptors, Neuropeptide - immunology</topic><topic>Receptors, Neuropeptide Y - immunology</topic><topic>Transplantion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schmitz, Jessica</creatorcontrib><creatorcontrib>Zakrzewicz, Anna</creatorcontrib><creatorcontrib>Wilker, Sigrid</creatorcontrib><creatorcontrib>Kuncová, Jitka</creatorcontrib><creatorcontrib>Hecker, Andreas</creatorcontrib><creatorcontrib>Grau, Veronika</creatorcontrib><creatorcontrib>Padberg, Winfried</creatorcontrib><creatorcontrib>Holler, Julia P.N</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Transplant immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schmitz, Jessica</au><au>Zakrzewicz, Anna</au><au>Wilker, Sigrid</au><au>Kuncová, Jitka</au><au>Hecker, Andreas</au><au>Grau, Veronika</au><au>Padberg, Winfried</au><au>Holler, Julia P.N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Non-neuronal neuropeptide Y and its receptors during acute rejection of rat pulmonary allografts</atitle><jtitle>Transplant immunology</jtitle><addtitle>Transpl Immunol</addtitle><date>2017-08-01</date><risdate>2017</risdate><volume>43-44</volume><spage>49</spage><epage>53</epage><pages>49-53</pages><issn>0966-3274</issn><eissn>1878-5492</eissn><abstract>Abstract This study tested the hypothesis that neuropeptide Y (NPY) and NPY receptors 1 (Y1) and 2 (Y2) participate in lung allograft rejection. Inflammation in grafts may include interaction between blood leukocytes and graft endothelial cells and marked accumulation of intravascular blood leukocytes. Fewer leukocytes accumulate in lung than in kidney allografts. Lung transplantion was performed in the Dark Agouti to Lewis rat strain combination. Intravascular and intraalveolar leukocytes were isolated from the grafts, and we evaluated the mRNA expression of NPY, Y1, and Y2 by real-time RT-PCR as well as the peptide expression of NPY by radioimmunoassay and immunohistochemistry. NPY and Y1 were expressed by pulmonary intravascular and intraalveolar leukocytes. Y1 was up-regulated by pulmonary intravascular and intraalveolar leukocytes during allograft rejection while Y2 could not be detected. Higher NPY expression levels in intravascular leukocytes were observed in lung compared to kidney allografts, which were investigated previously. Our findings suggest that an increased leukocytic expression of NPY in lung compared to kidney allografts results in a reduced accumulation of leukocytes in allograft vessels.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>28438668</pmid><doi>10.1016/j.trim.2017.04.004</doi><tpages>5</tpages></addata></record> |
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| subjects | Acute Disease Allergy and Immunology Allografts Animals Gene Expression Regulation - immunology Graft Rejection - immunology Graft Rejection - pathology Immunosuppression Inflammation Leukocytes Leukocytes - immunology Leukocytes - pathology Lung - immunology Lung - pathology Lung Transplantation Neuropeptide Y Neuropeptide Y - immunology Rats Rats, Inbred Lew Receptors, G-Protein-Coupled - immunology Receptors, Neuropeptide - immunology Receptors, Neuropeptide Y - immunology Transplantion |
| title | Non-neuronal neuropeptide Y and its receptors during acute rejection of rat pulmonary allografts |
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