Inositolphosphoglycans and diacylglycerol are possible mediators in the glycogenic effect of GLP-1(7-36)amide in BC3H-1 myocytes
A potent glycogenic effect of GLP‐1(7‐36)amide has been found in rat hepatocytes and skeletal muscle, and specific receptors for this peptide, which do not seem to be associated with the adenylate cyclase—cAMP system, have been detected in these tissue membranes. On the other hand, inositolphosphogl...
Gespeichert in:
| Veröffentlicht in: | Cell biochemistry and function 1996-03, Vol.14 (1), p.43-48 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 48 |
|---|---|
| container_issue | 1 |
| container_start_page | 43 |
| container_title | Cell biochemistry and function |
| container_volume | 14 |
| creator | Galera, C. Clemente, F. Alcántara, A. Trapote, M. A. Perea, A. López-Delgado, M. I. Villanueva-Peñacarrillo, M. L. Valverde, I. |
| description | A potent glycogenic effect of GLP‐1(7‐36)amide has been found in rat hepatocytes and skeletal muscle, and specific receptors for this peptide, which do not seem to be associated with the adenylate cyclase—cAMP system, have been detected in these tissue membranes. On the other hand, inositolphosphoglycan molecules (IPGs) have been implicated as second messengers of the action of insulin. In this work, we have found, in differentiated BC3H‐1 myocytes, specific binding of [125I]GLP‐1(7‐36)amide, and a stimulatory effect of the peptide on glycogen synthesis, confirming the findings in rat skeletal muscle. Also, GLP‐1(7‐36)amide modulates the cell content of radiolabelled glycosylphosphatidylinositols (GPIs) and increases the production of diacylglycerol (DAG), in the same manner as insulin acts, indicating hydrolysis of GPIs and an immediate and short‐lived generation of IPGs. Thus, IPGs and DAG could be mediators in the glycogenic action of GLP‐1(7‐36)amide in skeletal muscle. |
| doi_str_mv | 10.1002/cbf.639 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1891881778</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1891881778</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3989-2d0f169fd08f8198196b2a82ed230686328af0b21260fac91f5126286315c2d33</originalsourceid><addsrcrecordid>eNp10F1PHCEUBmBiatKtbfoXuKvGYPnYZeBSN7pq1rYX_fCOsMxBqcywwph27vrTy2aMdyacQDhPTnJehD4yesIo5Z_dxp9IoffQjFGtCVXz-Rs0o1wKIudq_ha9K-U3pVRLQWfo31WfShhS3N6nUusujs72Bdu-xW2wboy7H8gpYpsBb1MpYRMBd1C7Q8oFhx4P94B3LN1BHxwG78ENOHm8Wn8j7LAhQh7ZLrSww2dLcUkY7sbkxgHKe7TvbSzw4fk-QD8uzr8vL8n66-pqebomTmilCW-pZ1L7liqvmK5HbrhVHFouqFRScGU93XDGJfXWaeYX9clrgy0cb4U4QIfT3G1Oj09QBtOF4iBG20N6KoYpzZRiTaMq_TRRl-u6GbzZ5tDZPBpGzS5jUzM2NeMqjyf5J0QYX2NmeXYxaTLpUAb4-6JtfjCyEc3C_PqyMtfrG_6zudHmVvwHzj-KyQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1891881778</pqid></control><display><type>article</type><title>Inositolphosphoglycans and diacylglycerol are possible mediators in the glycogenic effect of GLP-1(7-36)amide in BC3H-1 myocytes</title><source>Access via Wiley Online Library</source><creator>Galera, C. ; Clemente, F. ; Alcántara, A. ; Trapote, M. A. ; Perea, A. ; López-Delgado, M. I. ; Villanueva-Peñacarrillo, M. L. ; Valverde, I.</creator><creatorcontrib>Galera, C. ; Clemente, F. ; Alcántara, A. ; Trapote, M. A. ; Perea, A. ; López-Delgado, M. I. ; Villanueva-Peñacarrillo, M. L. ; Valverde, I.</creatorcontrib><description>A potent glycogenic effect of GLP‐1(7‐36)amide has been found in rat hepatocytes and skeletal muscle, and specific receptors for this peptide, which do not seem to be associated with the adenylate cyclase—cAMP system, have been detected in these tissue membranes. On the other hand, inositolphosphoglycan molecules (IPGs) have been implicated as second messengers of the action of insulin. In this work, we have found, in differentiated BC3H‐1 myocytes, specific binding of [125I]GLP‐1(7‐36)amide, and a stimulatory effect of the peptide on glycogen synthesis, confirming the findings in rat skeletal muscle. Also, GLP‐1(7‐36)amide modulates the cell content of radiolabelled glycosylphosphatidylinositols (GPIs) and increases the production of diacylglycerol (DAG), in the same manner as insulin acts, indicating hydrolysis of GPIs and an immediate and short‐lived generation of IPGs. Thus, IPGs and DAG could be mediators in the glycogenic action of GLP‐1(7‐36)amide in skeletal muscle.</description><identifier>ISSN: 0263-6484</identifier><identifier>EISSN: 1099-0844</identifier><identifier>DOI: 10.1002/cbf.639</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>diacylglycerol ; GLP-1 ; glycogenesis ; inositolphosphoglycans ; myocytes</subject><ispartof>Cell biochemistry and function, 1996-03, Vol.14 (1), p.43-48</ispartof><rights>Copyright © 1996 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3989-2d0f169fd08f8198196b2a82ed230686328af0b21260fac91f5126286315c2d33</citedby><cites>FETCH-LOGICAL-c3989-2d0f169fd08f8198196b2a82ed230686328af0b21260fac91f5126286315c2d33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcbf.639$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcbf.639$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Galera, C.</creatorcontrib><creatorcontrib>Clemente, F.</creatorcontrib><creatorcontrib>Alcántara, A.</creatorcontrib><creatorcontrib>Trapote, M. A.</creatorcontrib><creatorcontrib>Perea, A.</creatorcontrib><creatorcontrib>López-Delgado, M. I.</creatorcontrib><creatorcontrib>Villanueva-Peñacarrillo, M. L.</creatorcontrib><creatorcontrib>Valverde, I.</creatorcontrib><title>Inositolphosphoglycans and diacylglycerol are possible mediators in the glycogenic effect of GLP-1(7-36)amide in BC3H-1 myocytes</title><title>Cell biochemistry and function</title><addtitle>Cell Biochem. Funct</addtitle><description>A potent glycogenic effect of GLP‐1(7‐36)amide has been found in rat hepatocytes and skeletal muscle, and specific receptors for this peptide, which do not seem to be associated with the adenylate cyclase—cAMP system, have been detected in these tissue membranes. On the other hand, inositolphosphoglycan molecules (IPGs) have been implicated as second messengers of the action of insulin. In this work, we have found, in differentiated BC3H‐1 myocytes, specific binding of [125I]GLP‐1(7‐36)amide, and a stimulatory effect of the peptide on glycogen synthesis, confirming the findings in rat skeletal muscle. Also, GLP‐1(7‐36)amide modulates the cell content of radiolabelled glycosylphosphatidylinositols (GPIs) and increases the production of diacylglycerol (DAG), in the same manner as insulin acts, indicating hydrolysis of GPIs and an immediate and short‐lived generation of IPGs. Thus, IPGs and DAG could be mediators in the glycogenic action of GLP‐1(7‐36)amide in skeletal muscle.</description><subject>diacylglycerol</subject><subject>GLP-1</subject><subject>glycogenesis</subject><subject>inositolphosphoglycans</subject><subject>myocytes</subject><issn>0263-6484</issn><issn>1099-0844</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNp10F1PHCEUBmBiatKtbfoXuKvGYPnYZeBSN7pq1rYX_fCOsMxBqcywwph27vrTy2aMdyacQDhPTnJehD4yesIo5Z_dxp9IoffQjFGtCVXz-Rs0o1wKIudq_ha9K-U3pVRLQWfo31WfShhS3N6nUusujs72Bdu-xW2wboy7H8gpYpsBb1MpYRMBd1C7Q8oFhx4P94B3LN1BHxwG78ENOHm8Wn8j7LAhQh7ZLrSww2dLcUkY7sbkxgHKe7TvbSzw4fk-QD8uzr8vL8n66-pqebomTmilCW-pZ1L7liqvmK5HbrhVHFouqFRScGU93XDGJfXWaeYX9clrgy0cb4U4QIfT3G1Oj09QBtOF4iBG20N6KoYpzZRiTaMq_TRRl-u6GbzZ5tDZPBpGzS5jUzM2NeMqjyf5J0QYX2NmeXYxaTLpUAb4-6JtfjCyEc3C_PqyMtfrG_6zudHmVvwHzj-KyQ</recordid><startdate>199603</startdate><enddate>199603</enddate><creator>Galera, C.</creator><creator>Clemente, F.</creator><creator>Alcántara, A.</creator><creator>Trapote, M. A.</creator><creator>Perea, A.</creator><creator>López-Delgado, M. I.</creator><creator>Villanueva-Peñacarrillo, M. L.</creator><creator>Valverde, I.</creator><general>John Wiley & Sons, Ltd</general><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>199603</creationdate><title>Inositolphosphoglycans and diacylglycerol are possible mediators in the glycogenic effect of GLP-1(7-36)amide in BC3H-1 myocytes</title><author>Galera, C. ; Clemente, F. ; Alcántara, A. ; Trapote, M. A. ; Perea, A. ; López-Delgado, M. I. ; Villanueva-Peñacarrillo, M. L. ; Valverde, I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3989-2d0f169fd08f8198196b2a82ed230686328af0b21260fac91f5126286315c2d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>diacylglycerol</topic><topic>GLP-1</topic><topic>glycogenesis</topic><topic>inositolphosphoglycans</topic><topic>myocytes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Galera, C.</creatorcontrib><creatorcontrib>Clemente, F.</creatorcontrib><creatorcontrib>Alcántara, A.</creatorcontrib><creatorcontrib>Trapote, M. A.</creatorcontrib><creatorcontrib>Perea, A.</creatorcontrib><creatorcontrib>López-Delgado, M. I.</creatorcontrib><creatorcontrib>Villanueva-Peñacarrillo, M. L.</creatorcontrib><creatorcontrib>Valverde, I.</creatorcontrib><collection>Istex</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Cell biochemistry and function</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Galera, C.</au><au>Clemente, F.</au><au>Alcántara, A.</au><au>Trapote, M. A.</au><au>Perea, A.</au><au>López-Delgado, M. I.</au><au>Villanueva-Peñacarrillo, M. L.</au><au>Valverde, I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inositolphosphoglycans and diacylglycerol are possible mediators in the glycogenic effect of GLP-1(7-36)amide in BC3H-1 myocytes</atitle><jtitle>Cell biochemistry and function</jtitle><addtitle>Cell Biochem. Funct</addtitle><date>1996-03</date><risdate>1996</risdate><volume>14</volume><issue>1</issue><spage>43</spage><epage>48</epage><pages>43-48</pages><issn>0263-6484</issn><eissn>1099-0844</eissn><abstract>A potent glycogenic effect of GLP‐1(7‐36)amide has been found in rat hepatocytes and skeletal muscle, and specific receptors for this peptide, which do not seem to be associated with the adenylate cyclase—cAMP system, have been detected in these tissue membranes. On the other hand, inositolphosphoglycan molecules (IPGs) have been implicated as second messengers of the action of insulin. In this work, we have found, in differentiated BC3H‐1 myocytes, specific binding of [125I]GLP‐1(7‐36)amide, and a stimulatory effect of the peptide on glycogen synthesis, confirming the findings in rat skeletal muscle. Also, GLP‐1(7‐36)amide modulates the cell content of radiolabelled glycosylphosphatidylinositols (GPIs) and increases the production of diacylglycerol (DAG), in the same manner as insulin acts, indicating hydrolysis of GPIs and an immediate and short‐lived generation of IPGs. Thus, IPGs and DAG could be mediators in the glycogenic action of GLP‐1(7‐36)amide in skeletal muscle.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/cbf.639</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0263-6484 |
| ispartof | Cell biochemistry and function, 1996-03, Vol.14 (1), p.43-48 |
| issn | 0263-6484 1099-0844 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1891881778 |
| source | Access via Wiley Online Library |
| subjects | diacylglycerol GLP-1 glycogenesis inositolphosphoglycans myocytes |
| title | Inositolphosphoglycans and diacylglycerol are possible mediators in the glycogenic effect of GLP-1(7-36)amide in BC3H-1 myocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T18%3A28%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Inositolphosphoglycans%20and%20diacylglycerol%20are%20possible%20mediators%20in%20the%20glycogenic%20effect%20of%20GLP-1(7-36)amide%20in%20BC3H-1%20myocytes&rft.jtitle=Cell%20biochemistry%20and%20function&rft.au=Galera,%20C.&rft.date=1996-03&rft.volume=14&rft.issue=1&rft.spage=43&rft.epage=48&rft.pages=43-48&rft.issn=0263-6484&rft.eissn=1099-0844&rft_id=info:doi/10.1002/cbf.639&rft_dat=%3Cproquest_cross%3E1891881778%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1891881778&rft_id=info:pmid/&rfr_iscdi=true |