Drought-inducible expression of Hv-miR827 enhances drought tolerance in transgenic barley
Drought is one of the major abiotic stresses reducing crop yield. Since the discovery of plant microRNAs (miRNAs), considerable progress has been made in clarifying their role in plant responses to abiotic stresses, including drought. miR827 was previously reported to confer drought tolerance in tra...
Gespeichert in:
| Veröffentlicht in: | Functional & integrative genomics 2017-05, Vol.17 (2-3), p.279-292 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 292 |
|---|---|
| container_issue | 2-3 |
| container_start_page | 279 |
| container_title | Functional & integrative genomics |
| container_volume | 17 |
| creator | Ferdous, Jannatul Whitford, Ryan Nguyen, Martin Brien, Chris Langridge, Peter Tricker, Penny J. |
| description | Drought is one of the major abiotic stresses reducing crop yield. Since the discovery of plant microRNAs (miRNAs), considerable progress has been made in clarifying their role in plant responses to abiotic stresses, including drought. miR827 was previously reported to confer drought tolerance in transgenic
Arabidopsis
. We examined barley (
Hordeum vulgare
L. ‘Golden Promise’) plants over-expressing miR827 for plant performance under drought. Transgenic plants constitutively expressing
CaMV-35S
::
Ath
-miR827 and drought-inducible
Zm-Rab17
::
Hv
-miR827 were phenotyped by non-destructive imaging for growth and whole plant water use efficiency (WUE
wp
). We observed that the growth, WUE
wp
, time to anthesis and grain weight of transgenic barley plants expressing
CaMV-35S
::
Ath
-miR827 were negatively affected in both well-watered and drought-treated growing conditions compared with the wild-type plants. In contrast, transgenic plants over-expressing
Zm-Rab17
::
Hv
-miR827 showed improved WUE
wp
with no growth or reproductive timing change compared with the wild-type plants. The recovery of
Zm-Rab17
::
Hv
-miR827 over-expressing plants also improved following severe drought stress. Our results suggest that
Hv
-miR827 has the potential to improve the performance of barley under drought and that the choice of promoter to control the timing and specificity of miRNA expression is critical. |
| doi_str_mv | 10.1007/s10142-016-0526-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1891876900</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1835406668</sourcerecordid><originalsourceid>FETCH-LOGICAL-c405t-d218fef366e537f75fe9e979b678d9aea19f96dc0a0e9a6829e302b4549eb4ad3</originalsourceid><addsrcrecordid>eNqNkU1r3DAQhkVJaT6aH5BLEOTSi9rRh_VxDEmaDQQKpYH0JGR7vOvglTeSHZp_Xy9OllAI9KRB88w7DA8hJxy-cgDzLXPgSjDgmkEhNLMfyAFX0jLjlN3b1fJ-nxzm_AAABTj5iewLYyQooQ_I78vUj8vVwNpYj1VbdkjxzyZhzm0fad_QxRNbtz-tMBTjKsQKM63nETr0HabtF20jHaYqLzG2FS1D6vD5M_nYhC7j8ct7RO6-X_26WLDbH9c3F-e3rFJQDKwW3DbYSK2xkKYxRYMOnXGlNrZ2AQN3jdN1BQHQBW2FQwmiVIVyWKpQyyPyZc7dpP5xxDz4dZsr7LoQsR-z59Zxa7QD-A9UFgq01nZCz_5BH_oxxemQibLKGFFwM1F8pqrU55yw8ZvUrkN69hz8VpGfFflJkd8q8tvk05fksVxjvZt4dTIBYgby1IpLTG9Wv5v6F8KQm0o</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1884772517</pqid></control><display><type>article</type><title>Drought-inducible expression of Hv-miR827 enhances drought tolerance in transgenic barley</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Ferdous, Jannatul ; Whitford, Ryan ; Nguyen, Martin ; Brien, Chris ; Langridge, Peter ; Tricker, Penny J.</creator><creatorcontrib>Ferdous, Jannatul ; Whitford, Ryan ; Nguyen, Martin ; Brien, Chris ; Langridge, Peter ; Tricker, Penny J.</creatorcontrib><description>Drought is one of the major abiotic stresses reducing crop yield. Since the discovery of plant microRNAs (miRNAs), considerable progress has been made in clarifying their role in plant responses to abiotic stresses, including drought. miR827 was previously reported to confer drought tolerance in transgenic
Arabidopsis
. We examined barley (
Hordeum vulgare
L. ‘Golden Promise’) plants over-expressing miR827 for plant performance under drought. Transgenic plants constitutively expressing
CaMV-35S
::
Ath
-miR827 and drought-inducible
Zm-Rab17
::
Hv
-miR827 were phenotyped by non-destructive imaging for growth and whole plant water use efficiency (WUE
wp
). We observed that the growth, WUE
wp
, time to anthesis and grain weight of transgenic barley plants expressing
CaMV-35S
::
Ath
-miR827 were negatively affected in both well-watered and drought-treated growing conditions compared with the wild-type plants. In contrast, transgenic plants over-expressing
Zm-Rab17
::
Hv
-miR827 showed improved WUE
wp
with no growth or reproductive timing change compared with the wild-type plants. The recovery of
Zm-Rab17
::
Hv
-miR827 over-expressing plants also improved following severe drought stress. Our results suggest that
Hv
-miR827 has the potential to improve the performance of barley under drought and that the choice of promoter to control the timing and specificity of miRNA expression is critical.</description><identifier>ISSN: 1438-793X</identifier><identifier>EISSN: 1438-7948</identifier><identifier>DOI: 10.1007/s10142-016-0526-8</identifier><identifier>PMID: 27730426</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Abiotic stress ; Adaptation, Physiological ; Animal Genetics and Genomics ; Arabidopsis ; Barley ; Biochemistry ; Bioinformatics ; Biomedical and Life Sciences ; Cell Biology ; Drought ; Droughts ; Gene expression ; Hordeum - genetics ; Hordeum vulgare ; Life Sciences ; Microbial Genetics and Genomics ; MicroRNAs ; Original Article ; Plant Genetics and Genomics ; Plants, Genetically Modified ; Transgenic plants</subject><ispartof>Functional & integrative genomics, 2017-05, Vol.17 (2-3), p.279-292</ispartof><rights>Springer-Verlag Berlin Heidelberg 2016</rights><rights>Functional & Integrative Genomics is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-d218fef366e537f75fe9e979b678d9aea19f96dc0a0e9a6829e302b4549eb4ad3</citedby><cites>FETCH-LOGICAL-c405t-d218fef366e537f75fe9e979b678d9aea19f96dc0a0e9a6829e302b4549eb4ad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10142-016-0526-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10142-016-0526-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27730426$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ferdous, Jannatul</creatorcontrib><creatorcontrib>Whitford, Ryan</creatorcontrib><creatorcontrib>Nguyen, Martin</creatorcontrib><creatorcontrib>Brien, Chris</creatorcontrib><creatorcontrib>Langridge, Peter</creatorcontrib><creatorcontrib>Tricker, Penny J.</creatorcontrib><title>Drought-inducible expression of Hv-miR827 enhances drought tolerance in transgenic barley</title><title>Functional & integrative genomics</title><addtitle>Funct Integr Genomics</addtitle><addtitle>Funct Integr Genomics</addtitle><description>Drought is one of the major abiotic stresses reducing crop yield. Since the discovery of plant microRNAs (miRNAs), considerable progress has been made in clarifying their role in plant responses to abiotic stresses, including drought. miR827 was previously reported to confer drought tolerance in transgenic
Arabidopsis
. We examined barley (
Hordeum vulgare
L. ‘Golden Promise’) plants over-expressing miR827 for plant performance under drought. Transgenic plants constitutively expressing
CaMV-35S
::
Ath
-miR827 and drought-inducible
Zm-Rab17
::
Hv
-miR827 were phenotyped by non-destructive imaging for growth and whole plant water use efficiency (WUE
wp
). We observed that the growth, WUE
wp
, time to anthesis and grain weight of transgenic barley plants expressing
CaMV-35S
::
Ath
-miR827 were negatively affected in both well-watered and drought-treated growing conditions compared with the wild-type plants. In contrast, transgenic plants over-expressing
Zm-Rab17
::
Hv
-miR827 showed improved WUE
wp
with no growth or reproductive timing change compared with the wild-type plants. The recovery of
Zm-Rab17
::
Hv
-miR827 over-expressing plants also improved following severe drought stress. Our results suggest that
Hv
-miR827 has the potential to improve the performance of barley under drought and that the choice of promoter to control the timing and specificity of miRNA expression is critical.</description><subject>Abiotic stress</subject><subject>Adaptation, Physiological</subject><subject>Animal Genetics and Genomics</subject><subject>Arabidopsis</subject><subject>Barley</subject><subject>Biochemistry</subject><subject>Bioinformatics</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Drought</subject><subject>Droughts</subject><subject>Gene expression</subject><subject>Hordeum - genetics</subject><subject>Hordeum vulgare</subject><subject>Life Sciences</subject><subject>Microbial Genetics and Genomics</subject><subject>MicroRNAs</subject><subject>Original Article</subject><subject>Plant Genetics and Genomics</subject><subject>Plants, Genetically Modified</subject><subject>Transgenic plants</subject><issn>1438-793X</issn><issn>1438-7948</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkU1r3DAQhkVJaT6aH5BLEOTSi9rRh_VxDEmaDQQKpYH0JGR7vOvglTeSHZp_Xy9OllAI9KRB88w7DA8hJxy-cgDzLXPgSjDgmkEhNLMfyAFX0jLjlN3b1fJ-nxzm_AAABTj5iewLYyQooQ_I78vUj8vVwNpYj1VbdkjxzyZhzm0fad_QxRNbtz-tMBTjKsQKM63nETr0HabtF20jHaYqLzG2FS1D6vD5M_nYhC7j8ct7RO6-X_26WLDbH9c3F-e3rFJQDKwW3DbYSK2xkKYxRYMOnXGlNrZ2AQN3jdN1BQHQBW2FQwmiVIVyWKpQyyPyZc7dpP5xxDz4dZsr7LoQsR-z59Zxa7QD-A9UFgq01nZCz_5BH_oxxemQibLKGFFwM1F8pqrU55yw8ZvUrkN69hz8VpGfFflJkd8q8tvk05fksVxjvZt4dTIBYgby1IpLTG9Wv5v6F8KQm0o</recordid><startdate>20170501</startdate><enddate>20170501</enddate><creator>Ferdous, Jannatul</creator><creator>Whitford, Ryan</creator><creator>Nguyen, Martin</creator><creator>Brien, Chris</creator><creator>Langridge, Peter</creator><creator>Tricker, Penny J.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PADUT</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20170501</creationdate><title>Drought-inducible expression of Hv-miR827 enhances drought tolerance in transgenic barley</title><author>Ferdous, Jannatul ; Whitford, Ryan ; Nguyen, Martin ; Brien, Chris ; Langridge, Peter ; Tricker, Penny J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-d218fef366e537f75fe9e979b678d9aea19f96dc0a0e9a6829e302b4549eb4ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Abiotic stress</topic><topic>Adaptation, Physiological</topic><topic>Animal Genetics and Genomics</topic><topic>Arabidopsis</topic><topic>Barley</topic><topic>Biochemistry</topic><topic>Bioinformatics</topic><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>Drought</topic><topic>Droughts</topic><topic>Gene expression</topic><topic>Hordeum - genetics</topic><topic>Hordeum vulgare</topic><topic>Life Sciences</topic><topic>Microbial Genetics and Genomics</topic><topic>MicroRNAs</topic><topic>Original Article</topic><topic>Plant Genetics and Genomics</topic><topic>Plants, Genetically Modified</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ferdous, Jannatul</creatorcontrib><creatorcontrib>Whitford, Ryan</creatorcontrib><creatorcontrib>Nguyen, Martin</creatorcontrib><creatorcontrib>Brien, Chris</creatorcontrib><creatorcontrib>Langridge, Peter</creatorcontrib><creatorcontrib>Tricker, Penny J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Research Library China</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Functional & integrative genomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ferdous, Jannatul</au><au>Whitford, Ryan</au><au>Nguyen, Martin</au><au>Brien, Chris</au><au>Langridge, Peter</au><au>Tricker, Penny J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Drought-inducible expression of Hv-miR827 enhances drought tolerance in transgenic barley</atitle><jtitle>Functional & integrative genomics</jtitle><stitle>Funct Integr Genomics</stitle><addtitle>Funct Integr Genomics</addtitle><date>2017-05-01</date><risdate>2017</risdate><volume>17</volume><issue>2-3</issue><spage>279</spage><epage>292</epage><pages>279-292</pages><issn>1438-793X</issn><eissn>1438-7948</eissn><abstract>Drought is one of the major abiotic stresses reducing crop yield. Since the discovery of plant microRNAs (miRNAs), considerable progress has been made in clarifying their role in plant responses to abiotic stresses, including drought. miR827 was previously reported to confer drought tolerance in transgenic
Arabidopsis
. We examined barley (
Hordeum vulgare
L. ‘Golden Promise’) plants over-expressing miR827 for plant performance under drought. Transgenic plants constitutively expressing
CaMV-35S
::
Ath
-miR827 and drought-inducible
Zm-Rab17
::
Hv
-miR827 were phenotyped by non-destructive imaging for growth and whole plant water use efficiency (WUE
wp
). We observed that the growth, WUE
wp
, time to anthesis and grain weight of transgenic barley plants expressing
CaMV-35S
::
Ath
-miR827 were negatively affected in both well-watered and drought-treated growing conditions compared with the wild-type plants. In contrast, transgenic plants over-expressing
Zm-Rab17
::
Hv
-miR827 showed improved WUE
wp
with no growth or reproductive timing change compared with the wild-type plants. The recovery of
Zm-Rab17
::
Hv
-miR827 over-expressing plants also improved following severe drought stress. Our results suggest that
Hv
-miR827 has the potential to improve the performance of barley under drought and that the choice of promoter to control the timing and specificity of miRNA expression is critical.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>27730426</pmid><doi>10.1007/s10142-016-0526-8</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1438-793X |
| ispartof | Functional & integrative genomics, 2017-05, Vol.17 (2-3), p.279-292 |
| issn | 1438-793X 1438-7948 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1891876900 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Abiotic stress Adaptation, Physiological Animal Genetics and Genomics Arabidopsis Barley Biochemistry Bioinformatics Biomedical and Life Sciences Cell Biology Drought Droughts Gene expression Hordeum - genetics Hordeum vulgare Life Sciences Microbial Genetics and Genomics MicroRNAs Original Article Plant Genetics and Genomics Plants, Genetically Modified Transgenic plants |
| title | Drought-inducible expression of Hv-miR827 enhances drought tolerance in transgenic barley |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T14%3A24%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Drought-inducible%20expression%20of%20Hv-miR827%20enhances%20drought%20tolerance%20in%20transgenic%20barley&rft.jtitle=Functional%20&%20integrative%20genomics&rft.au=Ferdous,%20Jannatul&rft.date=2017-05-01&rft.volume=17&rft.issue=2-3&rft.spage=279&rft.epage=292&rft.pages=279-292&rft.issn=1438-793X&rft.eissn=1438-7948&rft_id=info:doi/10.1007/s10142-016-0526-8&rft_dat=%3Cproquest_cross%3E1835406668%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1884772517&rft_id=info:pmid/27730426&rfr_iscdi=true |