The Influence of Ammonium, Nitrate, and Dissolved Oxygen Concentrations on Uptake, Nitrification, and Denitrification Rates Associated with Prairie Stream Substrata

Substrata samples were collected from Kings Creek on Konza Prairie Biological Station (Manhattan, Kansas) and incubated with varying levels of ammonium (NH4 +), nitrate (NO3 -), and dissolved oxygen (O2) to examine the response of nitrogen (N) uptake and transformation rates. Substrata collected wer...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Limnology and oceanography 2002-09, Vol.47 (5), p.1380-1393
Hauptverfasser:	Kemp, Melody J., Dodds, Walter K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal and plant ecology Animal, plant and microbial ecology Biological and medical sciences Bryophytes Diatoms Earth sciences Earth, ocean, space Exact sciences and technology Fresh water ecosystems Freshwater Fundamental and applied biological sciences. Psychology Geochemistry Green algae Hydrology Hydrology. Hydrogeology Lotic systems Mineralogy Nitrates Nitrification Nitrous oxide Prairies Quaternary ammonium compounds Silicates Streams Synecology Water geochemistry
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

container_end_page	1393
container_issue	5
container_start_page	1380
container_title	Limnology and oceanography
container_volume	47
creator	Kemp, Melody J. Dodds, Walter K.
description	Substrata samples were collected from Kings Creek on Konza Prairie Biological Station (Manhattan, Kansas) and incubated with varying levels of ammonium (NH4 +), nitrate (NO3 -), and dissolved oxygen (O2) to examine the response of nitrogen (N) uptake and transformation rates. Substrata collected were fine benthic organic matter (FBOM), coarse benthic organic matter, filamentous green algae, bryophytes, suspended particulate organic matter, and epilithic diatoms. Nitrification and denitrification were estimated by use of the nitrapyrin and acetylene inhibition methods, respectively. Ammonium uptake demonstrated Michaelis-Menten kinetics, with the highest maximum rates (Vmax) associated with filamentous green algae (5.90 mg N gdm-1d-1) and epilithic diatoms (4.96 m N gdm-1d-1). Nitrate uptake did not saturate at the highest NO3 -addition (25 µg N L-1) above ambient when associated with FBOM. Overall, maximum uptake rates of NH4 +were 10-fold higher than for NO3 -. Nitrification response to increasing NH4 +concentrations was highly variable, depending on the substrata type. Nitrification was lowest under low O2conditions, being undetectable when NO3 -was added but not when NH4 +was added. Denitrification increased linearly with NO3 -concentration when associated with epilithic diatoms and FBOM but became saturated at ~20 µg N L-1above ambient concentrations when associated with filamentous green algae. Samples purged with N2gas had the highest rates of denitrification. We predicted stream ecosystem rates using equations derived from the experimental data and substrata mass estimates measured in the field. Substantial temporal variability was predicted in uptake (0-1,300 mg NH4 +-N m-2d-1; 0-5.2 mg NO3 --N m-2d-1), nitrification (0-35 mg NH4 +-N m-2d-1), and denitrification (0-130 µg N2O-N m-2d-1) as due to natural variation in water column NH4 +,NO3 -, and O2concentrations.
doi_str_mv	10.4319/lo.2002.47.5.1380
format	Article
fullrecord	<record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_18908592</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>3068957</jstor_id><sourcerecordid>3068957</sourcerecordid><originalsourceid>FETCH-LOGICAL-a4450-8f81801bf79c6b992a40cae291495fbb8d82e8f813b9ed18a7e3f0698f27b2fa3</originalsourceid><addsrcrecordid>eNqFkc1u1DAURi0EEkPhAZBYeAOrJtixPbEXLEbDX6VRB9F2bTnJNXVx7MFOKPM-PCgJGQE7Vr6695zPiw-h55SUnFH12seyIqQqeV2KkjJJHqAVVUwVQijyEK2mGy_YND9GT3K-I4QoIcQK_by-BXwRrB8htICjxZu-j8GN_Tm-dEMyA5xjEzr81uUc_Xfo8P7H8QsEvI2TEGbCxZBxDPjmMJivsHjOuvb35WRD-HeJP0-5GW-myNZNY4fv3XCLPyXjkgN8NSQwPb4amzznm6fokTU-w7PTe4Zu3r-73n4sdvsPF9vNrjCcC1JIK6kktLG1ateNUpXhpDVQKcqVsE0jO1nBDLFGQUelqYFZslbSVnVTWcPO0Ksl95DitxHyoHuXW_DeBIhj1lQqIoWqJpAuYJtizgmsPiTXm3TUlOi5D-2jnvvQvNZCz31MzstTuMmt8TaZ0Lr8V2SKSiFm7s3C3TsPx_8H693lft7wWpz-ebH4d3mI6Y_PyFoqUbNfkFOpwQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18908592</pqid></control><display><type>article</type><title>The Influence of Ammonium, Nitrate, and Dissolved Oxygen Concentrations on Uptake, Nitrification, and Denitrification Rates Associated with Prairie Stream Substrata</title><source>Wiley Blackwell Single Titles</source><source>Wiley Online Library Free Content</source><source>Alma/SFX Local Collection</source><source>JSTOR</source><source>EZB Electronic Journals Library</source><creator>Kemp, Melody J. ; Dodds, Walter K.</creator><creatorcontrib>Kemp, Melody J. ; Dodds, Walter K.</creatorcontrib><description>Substrata samples were collected from Kings Creek on Konza Prairie Biological Station (Manhattan, Kansas) and incubated with varying levels of ammonium (NH4 +), nitrate (NO3 -), and dissolved oxygen (O2) to examine the response of nitrogen (N) uptake and transformation rates. Substrata collected were fine benthic organic matter (FBOM), coarse benthic organic matter, filamentous green algae, bryophytes, suspended particulate organic matter, and epilithic diatoms. Nitrification and denitrification were estimated by use of the nitrapyrin and acetylene inhibition methods, respectively. Ammonium uptake demonstrated Michaelis-Menten kinetics, with the highest maximum rates (Vmax) associated with filamentous green algae (5.90 mg N gdm-1d-1) and epilithic diatoms (4.96 m N gdm-1d-1). Nitrate uptake did not saturate at the highest NO3 -addition (25 µg N L-1) above ambient when associated with FBOM. Overall, maximum uptake rates of NH4 +were 10-fold higher than for NO3 -. Nitrification response to increasing NH4 +concentrations was highly variable, depending on the substrata type. Nitrification was lowest under low O2conditions, being undetectable when NO3 -was added but not when NH4 +was added. Denitrification increased linearly with NO3 -concentration when associated with epilithic diatoms and FBOM but became saturated at ~20 µg N L-1above ambient concentrations when associated with filamentous green algae. Samples purged with N2gas had the highest rates of denitrification. We predicted stream ecosystem rates using equations derived from the experimental data and substrata mass estimates measured in the field. Substantial temporal variability was predicted in uptake (0-1,300 mg NH4 +-N m-2d-1; 0-5.2 mg NO3 --N m-2d-1), nitrification (0-35 mg NH4 +-N m-2d-1), and denitrification (0-130 µg N2O-N m-2d-1) as due to natural variation in water column NH4 +,NO3 -, and O2concentrations.</description><identifier>ISSN: 0024-3590</identifier><identifier>EISSN: 1939-5590</identifier><identifier>DOI: 10.4319/lo.2002.47.5.1380</identifier><identifier>CODEN: LIOCAH</identifier><language>eng</language><publisher>Waco, TX: American Society of Limnology and Oceanography</publisher><subject>Animal and plant ecology ; Animal, plant and microbial ecology ; Biological and medical sciences ; Bryophytes ; Diatoms ; Earth sciences ; Earth, ocean, space ; Exact sciences and technology ; Fresh water ecosystems ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Geochemistry ; Green algae ; Hydrology ; Hydrology. Hydrogeology ; Lotic systems ; Mineralogy ; Nitrates ; Nitrification ; Nitrous oxide ; Prairies ; Quaternary ammonium compounds ; Silicates ; Streams ; Synecology ; Water geochemistry</subject><ispartof>Limnology and oceanography, 2002-09, Vol.47 (5), p.1380-1393</ispartof><rights>Copyright 2002 American Society of Limnology and Oceanography Inc.</rights><rights>2002, by the Association for the Sciences of Limnology and Oceanography, Inc.</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a4450-8f81801bf79c6b992a40cae291495fbb8d82e8f813b9ed18a7e3f0698f27b2fa3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3068957$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3068957$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,1416,1432,27915,27916,45565,45566,46400,46824,58008,58241</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13918550$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Kemp, Melody J.</creatorcontrib><creatorcontrib>Dodds, Walter K.</creatorcontrib><title>The Influence of Ammonium, Nitrate, and Dissolved Oxygen Concentrations on Uptake, Nitrification, and Denitrification Rates Associated with Prairie Stream Substrata</title><title>Limnology and oceanography</title><description>Substrata samples were collected from Kings Creek on Konza Prairie Biological Station (Manhattan, Kansas) and incubated with varying levels of ammonium (NH4 +), nitrate (NO3 -), and dissolved oxygen (O2) to examine the response of nitrogen (N) uptake and transformation rates. Substrata collected were fine benthic organic matter (FBOM), coarse benthic organic matter, filamentous green algae, bryophytes, suspended particulate organic matter, and epilithic diatoms. Nitrification and denitrification were estimated by use of the nitrapyrin and acetylene inhibition methods, respectively. Ammonium uptake demonstrated Michaelis-Menten kinetics, with the highest maximum rates (Vmax) associated with filamentous green algae (5.90 mg N gdm-1d-1) and epilithic diatoms (4.96 m N gdm-1d-1). Nitrate uptake did not saturate at the highest NO3 -addition (25 µg N L-1) above ambient when associated with FBOM. Overall, maximum uptake rates of NH4 +were 10-fold higher than for NO3 -. Nitrification response to increasing NH4 +concentrations was highly variable, depending on the substrata type. Nitrification was lowest under low O2conditions, being undetectable when NO3 -was added but not when NH4 +was added. Denitrification increased linearly with NO3 -concentration when associated with epilithic diatoms and FBOM but became saturated at ~20 µg N L-1above ambient concentrations when associated with filamentous green algae. Samples purged with N2gas had the highest rates of denitrification. We predicted stream ecosystem rates using equations derived from the experimental data and substrata mass estimates measured in the field. Substantial temporal variability was predicted in uptake (0-1,300 mg NH4 +-N m-2d-1; 0-5.2 mg NO3 --N m-2d-1), nitrification (0-35 mg NH4 +-N m-2d-1), and denitrification (0-130 µg N2O-N m-2d-1) as due to natural variation in water column NH4 +,NO3 -, and O2concentrations.</description><subject>Animal and plant ecology</subject><subject>Animal, plant and microbial ecology</subject><subject>Biological and medical sciences</subject><subject>Bryophytes</subject><subject>Diatoms</subject><subject>Earth sciences</subject><subject>Earth, ocean, space</subject><subject>Exact sciences and technology</subject><subject>Fresh water ecosystems</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Geochemistry</subject><subject>Green algae</subject><subject>Hydrology</subject><subject>Hydrology. Hydrogeology</subject><subject>Lotic systems</subject><subject>Mineralogy</subject><subject>Nitrates</subject><subject>Nitrification</subject><subject>Nitrous oxide</subject><subject>Prairies</subject><subject>Quaternary ammonium compounds</subject><subject>Silicates</subject><subject>Streams</subject><subject>Synecology</subject><subject>Water geochemistry</subject><issn>0024-3590</issn><issn>1939-5590</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqFkc1u1DAURi0EEkPhAZBYeAOrJtixPbEXLEbDX6VRB9F2bTnJNXVx7MFOKPM-PCgJGQE7Vr6695zPiw-h55SUnFH12seyIqQqeV2KkjJJHqAVVUwVQijyEK2mGy_YND9GT3K-I4QoIcQK_by-BXwRrB8htICjxZu-j8GN_Tm-dEMyA5xjEzr81uUc_Xfo8P7H8QsEvI2TEGbCxZBxDPjmMJivsHjOuvb35WRD-HeJP0-5GW-myNZNY4fv3XCLPyXjkgN8NSQwPb4amzznm6fokTU-w7PTe4Zu3r-73n4sdvsPF9vNrjCcC1JIK6kktLG1ateNUpXhpDVQKcqVsE0jO1nBDLFGQUelqYFZslbSVnVTWcPO0Ksl95DitxHyoHuXW_DeBIhj1lQqIoWqJpAuYJtizgmsPiTXm3TUlOi5D-2jnvvQvNZCz31MzstTuMmt8TaZ0Lr8V2SKSiFm7s3C3TsPx_8H693lft7wWpz-ebH4d3mI6Y_PyFoqUbNfkFOpwQ</recordid><startdate>200209</startdate><enddate>200209</enddate><creator>Kemp, Melody J.</creator><creator>Dodds, Walter K.</creator><general>American Society of Limnology and Oceanography</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7SN</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H96</scope><scope>L.G</scope><scope>M7N</scope></search><sort><creationdate>200209</creationdate><title>The Influence of Ammonium, Nitrate, and Dissolved Oxygen Concentrations on Uptake, Nitrification, and Denitrification Rates Associated with Prairie Stream Substrata</title><author>Kemp, Melody J. ; Dodds, Walter K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a4450-8f81801bf79c6b992a40cae291495fbb8d82e8f813b9ed18a7e3f0698f27b2fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animal and plant ecology</topic><topic>Animal, plant and microbial ecology</topic><topic>Biological and medical sciences</topic><topic>Bryophytes</topic><topic>Diatoms</topic><topic>Earth sciences</topic><topic>Earth, ocean, space</topic><topic>Exact sciences and technology</topic><topic>Fresh water ecosystems</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Geochemistry</topic><topic>Green algae</topic><topic>Hydrology</topic><topic>Hydrology. Hydrogeology</topic><topic>Lotic systems</topic><topic>Mineralogy</topic><topic>Nitrates</topic><topic>Nitrification</topic><topic>Nitrous oxide</topic><topic>Prairies</topic><topic>Quaternary ammonium compounds</topic><topic>Silicates</topic><topic>Streams</topic><topic>Synecology</topic><topic>Water geochemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kemp, Melody J.</creatorcontrib><creatorcontrib>Dodds, Walter K.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Ecology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 2: Ocean Technology, Policy & Non-Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Limnology and oceanography</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kemp, Melody J.</au><au>Dodds, Walter K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Influence of Ammonium, Nitrate, and Dissolved Oxygen Concentrations on Uptake, Nitrification, and Denitrification Rates Associated with Prairie Stream Substrata</atitle><jtitle>Limnology and oceanography</jtitle><date>2002-09</date><risdate>2002</risdate><volume>47</volume><issue>5</issue><spage>1380</spage><epage>1393</epage><pages>1380-1393</pages><issn>0024-3590</issn><eissn>1939-5590</eissn><coden>LIOCAH</coden><abstract>Substrata samples were collected from Kings Creek on Konza Prairie Biological Station (Manhattan, Kansas) and incubated with varying levels of ammonium (NH4 +), nitrate (NO3 -), and dissolved oxygen (O2) to examine the response of nitrogen (N) uptake and transformation rates. Substrata collected were fine benthic organic matter (FBOM), coarse benthic organic matter, filamentous green algae, bryophytes, suspended particulate organic matter, and epilithic diatoms. Nitrification and denitrification were estimated by use of the nitrapyrin and acetylene inhibition methods, respectively. Ammonium uptake demonstrated Michaelis-Menten kinetics, with the highest maximum rates (Vmax) associated with filamentous green algae (5.90 mg N gdm-1d-1) and epilithic diatoms (4.96 m N gdm-1d-1). Nitrate uptake did not saturate at the highest NO3 -addition (25 µg N L-1) above ambient when associated with FBOM. Overall, maximum uptake rates of NH4 +were 10-fold higher than for NO3 -. Nitrification response to increasing NH4 +concentrations was highly variable, depending on the substrata type. Nitrification was lowest under low O2conditions, being undetectable when NO3 -was added but not when NH4 +was added. Denitrification increased linearly with NO3 -concentration when associated with epilithic diatoms and FBOM but became saturated at ~20 µg N L-1above ambient concentrations when associated with filamentous green algae. Samples purged with N2gas had the highest rates of denitrification. We predicted stream ecosystem rates using equations derived from the experimental data and substrata mass estimates measured in the field. Substantial temporal variability was predicted in uptake (0-1,300 mg NH4 +-N m-2d-1; 0-5.2 mg NO3 --N m-2d-1), nitrification (0-35 mg NH4 +-N m-2d-1), and denitrification (0-130 µg N2O-N m-2d-1) as due to natural variation in water column NH4 +,NO3 -, and O2concentrations.</abstract><cop>Waco, TX</cop><pub>American Society of Limnology and Oceanography</pub><doi>10.4319/lo.2002.47.5.1380</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record>
fulltext	fulltext
identifier	ISSN: 0024-3590
ispartof	Limnology and oceanography, 2002-09, Vol.47 (5), p.1380-1393
issn	0024-3590 1939-5590
language	eng
recordid	cdi_proquest_miscellaneous_18908592
source	Wiley Blackwell Single Titles; Wiley Online Library Free Content; Alma/SFX Local Collection; JSTOR; EZB Electronic Journals Library
subjects	Animal and plant ecology Animal, plant and microbial ecology Biological and medical sciences Bryophytes Diatoms Earth sciences Earth, ocean, space Exact sciences and technology Fresh water ecosystems Freshwater Fundamental and applied biological sciences. Psychology Geochemistry Green algae Hydrology Hydrology. Hydrogeology Lotic systems Mineralogy Nitrates Nitrification Nitrous oxide Prairies Quaternary ammonium compounds Silicates Streams Synecology Water geochemistry
title	The Influence of Ammonium, Nitrate, and Dissolved Oxygen Concentrations on Uptake, Nitrification, and Denitrification Rates Associated with Prairie Stream Substrata
url	https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T19%3A57%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Influence%20of%20Ammonium,%20Nitrate,%20and%20Dissolved%20Oxygen%20Concentrations%20on%20Uptake,%20Nitrification,%20and%20Denitrification%20Rates%20Associated%20with%20Prairie%20Stream%20Substrata&rft.jtitle=Limnology%20and%20oceanography&rft.au=Kemp,%20Melody%20J.&rft.date=2002-09&rft.volume=47&rft.issue=5&rft.spage=1380&rft.epage=1393&rft.pages=1380-1393&rft.issn=0024-3590&rft.eissn=1939-5590&rft.coden=LIOCAH&rft_id=info:doi/10.4319/lo.2002.47.5.1380&rft_dat=%3Cjstor_proqu%3E3068957%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18908592&rft_id=info:pmid/&rft_jstor_id=3068957&rfr_iscdi=true