Aberrantly Expressed Long Non‐Coding RNAs In CD8+ T Cells Response to Active Tuberculosis
ABSTRACT Dysregulated expression of long noncoding RNAs (lncRNAs) has been demonstrated as being implicated in a variety of human diseases. In the study we aimed to determine lncRNA profile in CD8+ T cells response to active tuberculosis (TB). We examined the lncRNA expression by microarray in circu...
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| Veröffentlicht in: | Journal of cellular biochemistry 2017-12, Vol.118 (12), p.4275-4284 |
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| container_title | Journal of cellular biochemistry |
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| creator | Fu, Yurong Gao, Kunshan Tao, Enxue Li, Ruifang Yi, Zhengjun |
| description | ABSTRACT
Dysregulated expression of long noncoding RNAs (lncRNAs) has been demonstrated as being implicated in a variety of human diseases. In the study we aimed to determine lncRNA profile in CD8+ T cells response to active tuberculosis (TB). We examined the lncRNA expression by microarray in circulating CD8+ T cells isolated from patients with active TB and healthy controls. Change predictions to analysis was used to address functional roles of the deregulated mRNAs. Real‐time quantitative PCR (RT‐qPCR) was used to validate the microarray result. In total, 328 lncRNAs and 356 mRNAs were differentially expressed in TB CD8+ T cells. Upregulated mRNAs were mainly enriched in cAMP signaling pathway, calcium signaling pathway, and TGF‐beta signaling pathway, while downregulated mRNAs were enriched in antigen processing and presentation and natural killer cell mediated cytotoxicity in TB CD8+ T cells. Interestingly, we found that heme oxygenase 1 (HMOX1) was decreased in active TB CD8+ T cells, while its nearby lincRNA XLOC_014219 was upregulated. Subsequent RT‐qPCR results confirmed the changes. This is the first research addressing lncRNA expression profiles in active TB CD8+ T cells. The aberrantly expressed lncRNAs observed in the study may provide clues to the dysfunction of CD8+ T cells and so to the pathophysiological properties of active TB. Further studies should focus on the function of lncRNAs involved in active TB. J. Cell. Biochem. 118: 4275–4284, 2017. © 2017 Wiley Periodicals, Inc.
A total of 328 lncRNAs and 356 mRNAs were deregulated in circulating CD8+ T cells response to active TB. Heme oxygenase 1 (HMOX1) was decreased in active TB CD8+ T cells, while its nearby lincRNA XLOC_014219 was upregulated. |
| doi_str_mv | 10.1002/jcb.26078 |
| format | Article |
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Dysregulated expression of long noncoding RNAs (lncRNAs) has been demonstrated as being implicated in a variety of human diseases. In the study we aimed to determine lncRNA profile in CD8+ T cells response to active tuberculosis (TB). We examined the lncRNA expression by microarray in circulating CD8+ T cells isolated from patients with active TB and healthy controls. Change predictions to analysis was used to address functional roles of the deregulated mRNAs. Real‐time quantitative PCR (RT‐qPCR) was used to validate the microarray result. In total, 328 lncRNAs and 356 mRNAs were differentially expressed in TB CD8+ T cells. Upregulated mRNAs were mainly enriched in cAMP signaling pathway, calcium signaling pathway, and TGF‐beta signaling pathway, while downregulated mRNAs were enriched in antigen processing and presentation and natural killer cell mediated cytotoxicity in TB CD8+ T cells. Interestingly, we found that heme oxygenase 1 (HMOX1) was decreased in active TB CD8+ T cells, while its nearby lincRNA XLOC_014219 was upregulated. Subsequent RT‐qPCR results confirmed the changes. This is the first research addressing lncRNA expression profiles in active TB CD8+ T cells. The aberrantly expressed lncRNAs observed in the study may provide clues to the dysfunction of CD8+ T cells and so to the pathophysiological properties of active TB. Further studies should focus on the function of lncRNAs involved in active TB. J. Cell. Biochem. 118: 4275–4284, 2017. © 2017 Wiley Periodicals, Inc.
A total of 328 lncRNAs and 356 mRNAs were deregulated in circulating CD8+ T cells response to active TB. Heme oxygenase 1 (HMOX1) was decreased in active TB CD8+ T cells, while its nearby lincRNA XLOC_014219 was upregulated.</description><identifier>ISSN: 0730-2312</identifier><identifier>EISSN: 1097-4644</identifier><identifier>DOI: 10.1002/jcb.26078</identifier><identifier>PMID: 28422321</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Aberration ; Active control ; ACTIVE TUBERCULOSIS ; Adult ; Antigen presentation ; Antigen processing ; Calcium ; Calcium signalling ; CD8 antigen ; CD8+ T CELL ; CD8-Positive T-Lymphocytes - metabolism ; Cytotoxicity ; Deregulation ; Female ; Gene Expression Profiling ; Gene Expression Regulation ; Heme ; HMOX1 ; Humans ; LncRNA ; Lymphocytes ; Lymphocytes T ; Male ; Middle Aged ; Natural killer cells ; Oxygenase ; RNA, Long Noncoding - genetics ; Signal transduction ; SIGNALING PATHWAY ; T cell receptors ; Toxicity ; Transforming growth factor-b ; Tuberculosis ; Tuberculosis - genetics ; Tuberculosis - metabolism ; Young Adult</subject><ispartof>Journal of cellular biochemistry, 2017-12, Vol.118 (12), p.4275-4284</ispartof><rights>2017 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3538-a5e5202efb8cfebbfc43d49256c40a0ce2ae6d13807ef4c5c42900f7c48b5bd43</citedby><cites>FETCH-LOGICAL-c3538-a5e5202efb8cfebbfc43d49256c40a0ce2ae6d13807ef4c5c42900f7c48b5bd43</cites><orcidid>0000-0001-9350-2453</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcb.26078$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcb.26078$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27907,27908,45557,45558</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28422321$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fu, Yurong</creatorcontrib><creatorcontrib>Gao, Kunshan</creatorcontrib><creatorcontrib>Tao, Enxue</creatorcontrib><creatorcontrib>Li, Ruifang</creatorcontrib><creatorcontrib>Yi, Zhengjun</creatorcontrib><title>Aberrantly Expressed Long Non‐Coding RNAs In CD8+ T Cells Response to Active Tuberculosis</title><title>Journal of cellular biochemistry</title><addtitle>J Cell Biochem</addtitle><description>ABSTRACT
Dysregulated expression of long noncoding RNAs (lncRNAs) has been demonstrated as being implicated in a variety of human diseases. In the study we aimed to determine lncRNA profile in CD8+ T cells response to active tuberculosis (TB). We examined the lncRNA expression by microarray in circulating CD8+ T cells isolated from patients with active TB and healthy controls. Change predictions to analysis was used to address functional roles of the deregulated mRNAs. Real‐time quantitative PCR (RT‐qPCR) was used to validate the microarray result. In total, 328 lncRNAs and 356 mRNAs were differentially expressed in TB CD8+ T cells. Upregulated mRNAs were mainly enriched in cAMP signaling pathway, calcium signaling pathway, and TGF‐beta signaling pathway, while downregulated mRNAs were enriched in antigen processing and presentation and natural killer cell mediated cytotoxicity in TB CD8+ T cells. Interestingly, we found that heme oxygenase 1 (HMOX1) was decreased in active TB CD8+ T cells, while its nearby lincRNA XLOC_014219 was upregulated. Subsequent RT‐qPCR results confirmed the changes. This is the first research addressing lncRNA expression profiles in active TB CD8+ T cells. The aberrantly expressed lncRNAs observed in the study may provide clues to the dysfunction of CD8+ T cells and so to the pathophysiological properties of active TB. Further studies should focus on the function of lncRNAs involved in active TB. J. Cell. Biochem. 118: 4275–4284, 2017. © 2017 Wiley Periodicals, Inc.
A total of 328 lncRNAs and 356 mRNAs were deregulated in circulating CD8+ T cells response to active TB. Heme oxygenase 1 (HMOX1) was decreased in active TB CD8+ T cells, while its nearby lincRNA XLOC_014219 was upregulated.</description><subject>Aberration</subject><subject>Active control</subject><subject>ACTIVE TUBERCULOSIS</subject><subject>Adult</subject><subject>Antigen presentation</subject><subject>Antigen processing</subject><subject>Calcium</subject><subject>Calcium signalling</subject><subject>CD8 antigen</subject><subject>CD8+ T CELL</subject><subject>CD8-Positive T-Lymphocytes - metabolism</subject><subject>Cytotoxicity</subject><subject>Deregulation</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Heme</subject><subject>HMOX1</subject><subject>Humans</subject><subject>LncRNA</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Natural killer cells</subject><subject>Oxygenase</subject><subject>RNA, Long Noncoding - genetics</subject><subject>Signal transduction</subject><subject>SIGNALING PATHWAY</subject><subject>T cell receptors</subject><subject>Toxicity</subject><subject>Transforming growth factor-b</subject><subject>Tuberculosis</subject><subject>Tuberculosis - genetics</subject><subject>Tuberculosis - metabolism</subject><subject>Young Adult</subject><issn>0730-2312</issn><issn>1097-4644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1O20AURkdVUQm0C16gGqkbEDLc-bPHy2CgBUWpFKUrFiN7fF05cjxhJgay4xF4Rp6EaUNZIHV1daWjo0-HkAMGJwyAny5sdcJTyPQHMmKQZ4lMpfxIRpAJSLhgfJfshbAAgDwX_BPZ5VpyLjgbkZtxhd6X_brb0IuHlccQsKYT1_-mU9c_Pz4Vrm7jM5uOA73qaXGuj-mcFth1gc4wrFwfkK4dHdt1e4d0PkSfHToX2vCZ7DRlF_DL690nvy4v5sWPZPLz-1UxniRWKKGTUqHiwLGptG2wqhorRS1zrlIroQSLvMS0ZkJDho20ykqeAzSZlbpSVS3FPjncelfe3Q4Y1mbZBhsXlj26IRimdZ6lWmYQ0W_v0IUbfB_XGZYrwZXkikXqaEtZ70Lw2JiVb5el3xgG5k9xE4ubv8Uj-_XVOFRLrN_If4kjcLoF7tsON_83mevibKt8AdA5iVY</recordid><startdate>201712</startdate><enddate>201712</enddate><creator>Fu, Yurong</creator><creator>Gao, Kunshan</creator><creator>Tao, Enxue</creator><creator>Li, Ruifang</creator><creator>Yi, Zhengjun</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9350-2453</orcidid></search><sort><creationdate>201712</creationdate><title>Aberrantly Expressed Long Non‐Coding RNAs In CD8+ T Cells Response to Active Tuberculosis</title><author>Fu, Yurong ; Gao, Kunshan ; Tao, Enxue ; Li, Ruifang ; Yi, Zhengjun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3538-a5e5202efb8cfebbfc43d49256c40a0ce2ae6d13807ef4c5c42900f7c48b5bd43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Aberration</topic><topic>Active control</topic><topic>ACTIVE TUBERCULOSIS</topic><topic>Adult</topic><topic>Antigen presentation</topic><topic>Antigen processing</topic><topic>Calcium</topic><topic>Calcium signalling</topic><topic>CD8 antigen</topic><topic>CD8+ T CELL</topic><topic>CD8-Positive T-Lymphocytes - metabolism</topic><topic>Cytotoxicity</topic><topic>Deregulation</topic><topic>Female</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>Heme</topic><topic>HMOX1</topic><topic>Humans</topic><topic>LncRNA</topic><topic>Lymphocytes</topic><topic>Lymphocytes T</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Natural killer cells</topic><topic>Oxygenase</topic><topic>RNA, Long Noncoding - genetics</topic><topic>Signal transduction</topic><topic>SIGNALING PATHWAY</topic><topic>T cell receptors</topic><topic>Toxicity</topic><topic>Transforming growth factor-b</topic><topic>Tuberculosis</topic><topic>Tuberculosis - genetics</topic><topic>Tuberculosis - metabolism</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fu, Yurong</creatorcontrib><creatorcontrib>Gao, Kunshan</creatorcontrib><creatorcontrib>Tao, Enxue</creatorcontrib><creatorcontrib>Li, Ruifang</creatorcontrib><creatorcontrib>Yi, Zhengjun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fu, Yurong</au><au>Gao, Kunshan</au><au>Tao, Enxue</au><au>Li, Ruifang</au><au>Yi, Zhengjun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aberrantly Expressed Long Non‐Coding RNAs In CD8+ T Cells Response to Active Tuberculosis</atitle><jtitle>Journal of cellular biochemistry</jtitle><addtitle>J Cell Biochem</addtitle><date>2017-12</date><risdate>2017</risdate><volume>118</volume><issue>12</issue><spage>4275</spage><epage>4284</epage><pages>4275-4284</pages><issn>0730-2312</issn><eissn>1097-4644</eissn><abstract>ABSTRACT
Dysregulated expression of long noncoding RNAs (lncRNAs) has been demonstrated as being implicated in a variety of human diseases. In the study we aimed to determine lncRNA profile in CD8+ T cells response to active tuberculosis (TB). We examined the lncRNA expression by microarray in circulating CD8+ T cells isolated from patients with active TB and healthy controls. Change predictions to analysis was used to address functional roles of the deregulated mRNAs. Real‐time quantitative PCR (RT‐qPCR) was used to validate the microarray result. In total, 328 lncRNAs and 356 mRNAs were differentially expressed in TB CD8+ T cells. Upregulated mRNAs were mainly enriched in cAMP signaling pathway, calcium signaling pathway, and TGF‐beta signaling pathway, while downregulated mRNAs were enriched in antigen processing and presentation and natural killer cell mediated cytotoxicity in TB CD8+ T cells. Interestingly, we found that heme oxygenase 1 (HMOX1) was decreased in active TB CD8+ T cells, while its nearby lincRNA XLOC_014219 was upregulated. Subsequent RT‐qPCR results confirmed the changes. This is the first research addressing lncRNA expression profiles in active TB CD8+ T cells. The aberrantly expressed lncRNAs observed in the study may provide clues to the dysfunction of CD8+ T cells and so to the pathophysiological properties of active TB. Further studies should focus on the function of lncRNAs involved in active TB. J. Cell. Biochem. 118: 4275–4284, 2017. © 2017 Wiley Periodicals, Inc.
A total of 328 lncRNAs and 356 mRNAs were deregulated in circulating CD8+ T cells response to active TB. Heme oxygenase 1 (HMOX1) was decreased in active TB CD8+ T cells, while its nearby lincRNA XLOC_014219 was upregulated.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>28422321</pmid><doi>10.1002/jcb.26078</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-9350-2453</orcidid></addata></record> |
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| subjects | Aberration Active control ACTIVE TUBERCULOSIS Adult Antigen presentation Antigen processing Calcium Calcium signalling CD8 antigen CD8+ T CELL CD8-Positive T-Lymphocytes - metabolism Cytotoxicity Deregulation Female Gene Expression Profiling Gene Expression Regulation Heme HMOX1 Humans LncRNA Lymphocytes Lymphocytes T Male Middle Aged Natural killer cells Oxygenase RNA, Long Noncoding - genetics Signal transduction SIGNALING PATHWAY T cell receptors Toxicity Transforming growth factor-b Tuberculosis Tuberculosis - genetics Tuberculosis - metabolism Young Adult |
| title | Aberrantly Expressed Long Non‐Coding RNAs In CD8+ T Cells Response to Active Tuberculosis |
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