Glechoma hederacea extracts attenuate cholestatic liver injury in a bile duct-ligated rat model
In traditional Chinese medicine, Glechoma hederacea is frequently prescribed to patients with cholelithiasis, dropsy, abscess, diabetes, inflammation, and jaundice. Polyphenolic compounds are main bioactive components of Glechoma hederacea. This study was aimed to investigate the hepatoprotective po...
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| Veröffentlicht in: | Journal of ethnopharmacology 2017-05, Vol.204 (NA), p.58-66 |
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| description | In traditional Chinese medicine, Glechoma hederacea is frequently prescribed to patients with cholelithiasis, dropsy, abscess, diabetes, inflammation, and jaundice. Polyphenolic compounds are main bioactive components of Glechoma hederacea.
This study was aimed to investigate the hepatoprotective potential of hot water extract of Glechoma hederacea against cholestatic liver injury in rats.
Cholestatic liver injury was produced by ligating common bile ducts in Sprague-Dawley rats. Saline and hot water extract of Glechoma hederacea were orally administrated using gastric gavages. Liver tissues and bloods were collected and subjected to evaluation using histological, molecular, and biochemical approaches.
Using a rat model of cholestasis caused by bile duct ligation (BDL), daily oral administration of Glechoma hederacea hot water extracts showed protective effects against cholestatic liver injury, as evidenced by the improvement of serum biochemicals, ductular reaction, oxidative stress, inflammation, and fibrosis. Glechoma hederacea extracts alleviated BDL-induced transforming growth factor beta-1 (TGF-β1), connective tissue growth factor, and collagen expression, and the anti-fibrotic effects were accompanied by reductions in α-smooth muscle actin-positive matrix-producing cells and Smad2/3 activity. Glechoma hederacea extracts attenuated BDL-induced inflammatory cell infiltration/accumulation, NF-κB and AP-1 activation, and inflammatory cytokine production. Further studies demonstrated an inhibitory effect of Glechoma hederacea extracts on the axis of high mobility group box-1 (HMGB1)/toll-like receptor-4 (TLR4) intracellular signaling pathways.
The hepatoprotective, anti-oxidative, anti-inflammatory, and anti-fibrotic effects of Glechoma hederacea extracts seem to be multifactorial. The beneficial effects of daily Glechoma hederacea extracts supplementation were associated with anti-oxidative, anti-inflammatory, and anti-fibrotic potential, as well as down-regulation of NF-κB, AP-1, and TGF-β/Smad signaling, probably via interference with the HMGB1/TLR4 axis.
[Display omitted] |
| doi_str_mv | 10.1016/j.jep.2017.04.011 |
| format | Article |
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This study was aimed to investigate the hepatoprotective potential of hot water extract of Glechoma hederacea against cholestatic liver injury in rats.
Cholestatic liver injury was produced by ligating common bile ducts in Sprague-Dawley rats. Saline and hot water extract of Glechoma hederacea were orally administrated using gastric gavages. Liver tissues and bloods were collected and subjected to evaluation using histological, molecular, and biochemical approaches.
Using a rat model of cholestasis caused by bile duct ligation (BDL), daily oral administration of Glechoma hederacea hot water extracts showed protective effects against cholestatic liver injury, as evidenced by the improvement of serum biochemicals, ductular reaction, oxidative stress, inflammation, and fibrosis. Glechoma hederacea extracts alleviated BDL-induced transforming growth factor beta-1 (TGF-β1), connective tissue growth factor, and collagen expression, and the anti-fibrotic effects were accompanied by reductions in α-smooth muscle actin-positive matrix-producing cells and Smad2/3 activity. Glechoma hederacea extracts attenuated BDL-induced inflammatory cell infiltration/accumulation, NF-κB and AP-1 activation, and inflammatory cytokine production. Further studies demonstrated an inhibitory effect of Glechoma hederacea extracts on the axis of high mobility group box-1 (HMGB1)/toll-like receptor-4 (TLR4) intracellular signaling pathways.
The hepatoprotective, anti-oxidative, anti-inflammatory, and anti-fibrotic effects of Glechoma hederacea extracts seem to be multifactorial. The beneficial effects of daily Glechoma hederacea extracts supplementation were associated with anti-oxidative, anti-inflammatory, and anti-fibrotic potential, as well as down-regulation of NF-κB, AP-1, and TGF-β/Smad signaling, probably via interference with the HMGB1/TLR4 axis.
[Display omitted]</description><identifier>ISSN: 0378-8741</identifier><identifier>EISSN: 1872-7573</identifier><identifier>DOI: 10.1016/j.jep.2017.04.011</identifier><identifier>PMID: 28416441</identifier><language>eng</language><publisher>Ireland: Elsevier B.V</publisher><subject>Animals ; Anti-Inflammatory Agents - pharmacology ; Anti-Inflammatory Agents - therapeutic use ; Antioxidants - pharmacology ; Antioxidants - therapeutic use ; Bile Ducts - surgery ; Cholestasis ; Cholestasis - drug therapy ; Cholestasis - metabolism ; Cholestasis - pathology ; Collagen - metabolism ; Connective Tissue Growth Factor - metabolism ; Fibrosis ; Herbal medicine ; Lamiaceae ; Ligation ; Liver - drug effects ; Liver - metabolism ; Liver - pathology ; Male ; NF-kappa B - metabolism ; Phytotherapy ; Plant Extracts - pharmacology ; Plant Extracts - therapeutic use ; Polyphenol ; Rats, Sprague-Dawley ; Rosmarinic acid ; Smad2 Protein - metabolism ; Smad3 Protein - metabolism ; Transcription Factor AP-1 - metabolism ; Transforming Growth Factor beta1 - metabolism</subject><ispartof>Journal of ethnopharmacology, 2017-05, Vol.204 (NA), p.58-66</ispartof><rights>2017 Elsevier Ireland Ltd</rights><rights>Copyright © 2017 Elsevier Ireland Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-440ae4a570ef7ff082be915ff9318fa32e30b4ca7aed9878ab3460b0a3ad2acb3</citedby><cites>FETCH-LOGICAL-c452t-440ae4a570ef7ff082be915ff9318fa32e30b4ca7aed9878ab3460b0a3ad2acb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378874117300880$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28416441$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Ya-Yu</creatorcontrib><creatorcontrib>Lin, Shih-Yi</creatorcontrib><creatorcontrib>Chen, Wen-Ying</creatorcontrib><creatorcontrib>Liao, Su-Lan</creatorcontrib><creatorcontrib>Wu, Chih-Cheng</creatorcontrib><creatorcontrib>Pan, Pin-Ho</creatorcontrib><creatorcontrib>Chou, Su-Tze</creatorcontrib><creatorcontrib>Chen, Chun-Jung</creatorcontrib><title>Glechoma hederacea extracts attenuate cholestatic liver injury in a bile duct-ligated rat model</title><title>Journal of ethnopharmacology</title><addtitle>J Ethnopharmacol</addtitle><description>In traditional Chinese medicine, Glechoma hederacea is frequently prescribed to patients with cholelithiasis, dropsy, abscess, diabetes, inflammation, and jaundice. Polyphenolic compounds are main bioactive components of Glechoma hederacea.
This study was aimed to investigate the hepatoprotective potential of hot water extract of Glechoma hederacea against cholestatic liver injury in rats.
Cholestatic liver injury was produced by ligating common bile ducts in Sprague-Dawley rats. Saline and hot water extract of Glechoma hederacea were orally administrated using gastric gavages. Liver tissues and bloods were collected and subjected to evaluation using histological, molecular, and biochemical approaches.
Using a rat model of cholestasis caused by bile duct ligation (BDL), daily oral administration of Glechoma hederacea hot water extracts showed protective effects against cholestatic liver injury, as evidenced by the improvement of serum biochemicals, ductular reaction, oxidative stress, inflammation, and fibrosis. Glechoma hederacea extracts alleviated BDL-induced transforming growth factor beta-1 (TGF-β1), connective tissue growth factor, and collagen expression, and the anti-fibrotic effects were accompanied by reductions in α-smooth muscle actin-positive matrix-producing cells and Smad2/3 activity. Glechoma hederacea extracts attenuated BDL-induced inflammatory cell infiltration/accumulation, NF-κB and AP-1 activation, and inflammatory cytokine production. Further studies demonstrated an inhibitory effect of Glechoma hederacea extracts on the axis of high mobility group box-1 (HMGB1)/toll-like receptor-4 (TLR4) intracellular signaling pathways.
The hepatoprotective, anti-oxidative, anti-inflammatory, and anti-fibrotic effects of Glechoma hederacea extracts seem to be multifactorial. The beneficial effects of daily Glechoma hederacea extracts supplementation were associated with anti-oxidative, anti-inflammatory, and anti-fibrotic potential, as well as down-regulation of NF-κB, AP-1, and TGF-β/Smad signaling, probably via interference with the HMGB1/TLR4 axis.
[Display omitted]</description><subject>Animals</subject><subject>Anti-Inflammatory Agents - pharmacology</subject><subject>Anti-Inflammatory Agents - therapeutic use</subject><subject>Antioxidants - pharmacology</subject><subject>Antioxidants - therapeutic use</subject><subject>Bile Ducts - surgery</subject><subject>Cholestasis</subject><subject>Cholestasis - drug therapy</subject><subject>Cholestasis - metabolism</subject><subject>Cholestasis - pathology</subject><subject>Collagen - metabolism</subject><subject>Connective Tissue Growth Factor - metabolism</subject><subject>Fibrosis</subject><subject>Herbal medicine</subject><subject>Lamiaceae</subject><subject>Ligation</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>Liver - pathology</subject><subject>Male</subject><subject>NF-kappa B - metabolism</subject><subject>Phytotherapy</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Extracts - therapeutic use</subject><subject>Polyphenol</subject><subject>Rats, Sprague-Dawley</subject><subject>Rosmarinic acid</subject><subject>Smad2 Protein - metabolism</subject><subject>Smad3 Protein - metabolism</subject><subject>Transcription Factor AP-1 - metabolism</subject><subject>Transforming Growth Factor beta1 - metabolism</subject><issn>0378-8741</issn><issn>1872-7573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EotvCD-CCfOSSMI6dtSNOqIJSqRIXOFsTe0IdOZvFdir67_FqC0c4vTl872n0HmNvBLQCxP793M50bDsQugXVghDP2E4Y3TW61_I524HUpjFaiQt2mfMMAFooeMkuOqPEXimxY_YmkrtfF-T35CmhI-T0q9SjZI6l0GHDQrwikXLBEhyP4YESD4d5S49VOPIxROJ-c6WJ4UfFPU9Y-LJ6iq_YiwljptdPesW-f_707fpLc_f15vb6413jVN-VRilAUthroElPE5hupEH00zRIYSaUHUkYlUON5AejDY5S7WEElOg7dKO8Yu_Ouce0_tzqq3YJ2VGMeKB1y7YDI4a-mvR_UWHMIM1edCdUnFGX1pwTTfaYwoLp0QqwpwnsbOsE9jSBBWXrBNXz9il-Gxfyfx1_Oq_AhzNAtY-HQMlmF-jgyIdErli_hn_E_wZzJpe8</recordid><startdate>20170523</startdate><enddate>20170523</enddate><creator>Wang, Ya-Yu</creator><creator>Lin, Shih-Yi</creator><creator>Chen, Wen-Ying</creator><creator>Liao, Su-Lan</creator><creator>Wu, Chih-Cheng</creator><creator>Pan, Pin-Ho</creator><creator>Chou, Su-Tze</creator><creator>Chen, Chun-Jung</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20170523</creationdate><title>Glechoma hederacea extracts attenuate cholestatic liver injury in a bile duct-ligated rat model</title><author>Wang, Ya-Yu ; Lin, Shih-Yi ; Chen, Wen-Ying ; Liao, Su-Lan ; Wu, Chih-Cheng ; Pan, Pin-Ho ; Chou, Su-Tze ; Chen, Chun-Jung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-440ae4a570ef7ff082be915ff9318fa32e30b4ca7aed9878ab3460b0a3ad2acb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Anti-Inflammatory Agents - pharmacology</topic><topic>Anti-Inflammatory Agents - therapeutic use</topic><topic>Antioxidants - pharmacology</topic><topic>Antioxidants - therapeutic use</topic><topic>Bile Ducts - surgery</topic><topic>Cholestasis</topic><topic>Cholestasis - drug therapy</topic><topic>Cholestasis - metabolism</topic><topic>Cholestasis - pathology</topic><topic>Collagen - metabolism</topic><topic>Connective Tissue Growth Factor - metabolism</topic><topic>Fibrosis</topic><topic>Herbal medicine</topic><topic>Lamiaceae</topic><topic>Ligation</topic><topic>Liver - drug effects</topic><topic>Liver - metabolism</topic><topic>Liver - pathology</topic><topic>Male</topic><topic>NF-kappa B - metabolism</topic><topic>Phytotherapy</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Extracts - therapeutic use</topic><topic>Polyphenol</topic><topic>Rats, Sprague-Dawley</topic><topic>Rosmarinic acid</topic><topic>Smad2 Protein - metabolism</topic><topic>Smad3 Protein - metabolism</topic><topic>Transcription Factor AP-1 - metabolism</topic><topic>Transforming Growth Factor beta1 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Ya-Yu</creatorcontrib><creatorcontrib>Lin, Shih-Yi</creatorcontrib><creatorcontrib>Chen, Wen-Ying</creatorcontrib><creatorcontrib>Liao, Su-Lan</creatorcontrib><creatorcontrib>Wu, Chih-Cheng</creatorcontrib><creatorcontrib>Pan, Pin-Ho</creatorcontrib><creatorcontrib>Chou, Su-Tze</creatorcontrib><creatorcontrib>Chen, Chun-Jung</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of ethnopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Ya-Yu</au><au>Lin, Shih-Yi</au><au>Chen, Wen-Ying</au><au>Liao, Su-Lan</au><au>Wu, Chih-Cheng</au><au>Pan, Pin-Ho</au><au>Chou, Su-Tze</au><au>Chen, Chun-Jung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glechoma hederacea extracts attenuate cholestatic liver injury in a bile duct-ligated rat model</atitle><jtitle>Journal of ethnopharmacology</jtitle><addtitle>J Ethnopharmacol</addtitle><date>2017-05-23</date><risdate>2017</risdate><volume>204</volume><issue>NA</issue><spage>58</spage><epage>66</epage><pages>58-66</pages><issn>0378-8741</issn><eissn>1872-7573</eissn><abstract>In traditional Chinese medicine, Glechoma hederacea is frequently prescribed to patients with cholelithiasis, dropsy, abscess, diabetes, inflammation, and jaundice. Polyphenolic compounds are main bioactive components of Glechoma hederacea.
This study was aimed to investigate the hepatoprotective potential of hot water extract of Glechoma hederacea against cholestatic liver injury in rats.
Cholestatic liver injury was produced by ligating common bile ducts in Sprague-Dawley rats. Saline and hot water extract of Glechoma hederacea were orally administrated using gastric gavages. Liver tissues and bloods were collected and subjected to evaluation using histological, molecular, and biochemical approaches.
Using a rat model of cholestasis caused by bile duct ligation (BDL), daily oral administration of Glechoma hederacea hot water extracts showed protective effects against cholestatic liver injury, as evidenced by the improvement of serum biochemicals, ductular reaction, oxidative stress, inflammation, and fibrosis. Glechoma hederacea extracts alleviated BDL-induced transforming growth factor beta-1 (TGF-β1), connective tissue growth factor, and collagen expression, and the anti-fibrotic effects were accompanied by reductions in α-smooth muscle actin-positive matrix-producing cells and Smad2/3 activity. Glechoma hederacea extracts attenuated BDL-induced inflammatory cell infiltration/accumulation, NF-κB and AP-1 activation, and inflammatory cytokine production. Further studies demonstrated an inhibitory effect of Glechoma hederacea extracts on the axis of high mobility group box-1 (HMGB1)/toll-like receptor-4 (TLR4) intracellular signaling pathways.
The hepatoprotective, anti-oxidative, anti-inflammatory, and anti-fibrotic effects of Glechoma hederacea extracts seem to be multifactorial. The beneficial effects of daily Glechoma hederacea extracts supplementation were associated with anti-oxidative, anti-inflammatory, and anti-fibrotic potential, as well as down-regulation of NF-κB, AP-1, and TGF-β/Smad signaling, probably via interference with the HMGB1/TLR4 axis.
[Display omitted]</abstract><cop>Ireland</cop><pub>Elsevier B.V</pub><pmid>28416441</pmid><doi>10.1016/j.jep.2017.04.011</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Anti-Inflammatory Agents - pharmacology Anti-Inflammatory Agents - therapeutic use Antioxidants - pharmacology Antioxidants - therapeutic use Bile Ducts - surgery Cholestasis Cholestasis - drug therapy Cholestasis - metabolism Cholestasis - pathology Collagen - metabolism Connective Tissue Growth Factor - metabolism Fibrosis Herbal medicine Lamiaceae Ligation Liver - drug effects Liver - metabolism Liver - pathology Male NF-kappa B - metabolism Phytotherapy Plant Extracts - pharmacology Plant Extracts - therapeutic use Polyphenol Rats, Sprague-Dawley Rosmarinic acid Smad2 Protein - metabolism Smad3 Protein - metabolism Transcription Factor AP-1 - metabolism Transforming Growth Factor beta1 - metabolism |
| title | Glechoma hederacea extracts attenuate cholestatic liver injury in a bile duct-ligated rat model |
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