Effects of riboflavin and ultraviolet light treatment on platelet thrombus formation and thrombus stability on collagen
BACKGROUND Pathogen reduction technologies (PRTs) are considered for the implementation of safer platelet (PLT) transfusion. PRT treatment involves the addition of a photosensitizer to a blood component followed by ultraviolet (UV) light irradiation. However, the effects of PRT treatment on PLT thro...
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| Veröffentlicht in: | Transfusion (Philadelphia, Pa.) Pa.), 2017-07, Vol.57 (7), p.1772-1780 |
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| creator | Terada, Chikahiro Shiba, Masayuki Nagai, Tadashi Satake, Masahiro |
| description | BACKGROUND
Pathogen reduction technologies (PRTs) are considered for the implementation of safer platelet (PLT) transfusion. PRT treatment involves the addition of a photosensitizer to a blood component followed by ultraviolet (UV) light irradiation. However, the effects of PRT treatment on PLT thrombus formation and thrombus stability have not been satisfactorily clarified.
STUDY DESIGN AND METHODS
Leukoreduced PLT concentrates (PCs) were treated with riboflavin and UV light (Mirasol PRT). PLT thrombus formation on collagen was evaluated by the microchannel method, by which the total amount of PLTs deposited was measured as indices of thrombus formation and thrombus stability. Using a cone‐plate shear‐induced PLT aggregometer, PLT reactivity in blood flow was examined in a wide range of shear stresses of 6 to 108 dyn/cm2.
RESULTS
There was no significant difference in surface coverage between PRT‐treated PLTs and control PLTs on collagen. On the other hand, the total amount of PRT‐treated PLTs deposited was higher than that of control PLTs. The promotive effect of PRT treatment on PLT deposition completely disappeared in the presence of tirofiban, a potent integrin αIIbβ3 inhibitor. The percentage of the dissociation of PRT‐treated PLTs on collagen was lower than that of control PLTs after flushing with phosphate‐buffered saline. PRT treatment significantly inhibited PLT aggregation under high‐shear‐stress conditions.
CONCLUSION
Riboflavin‐based PRT treatment of PCs leads to the enhancement of PLT thrombus formation and thrombus stability on collagen. However, it does not enhance the reactivity of PLTs not in contact with collagen under high‐shear‐stress conditions. |
| doi_str_mv | 10.1111/trf.14114 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1889385232</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1889385232</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3884-40c2b62c9d0b5fbb157d857236e5a1aae10888a8bb838e39a0dd15602ece58d93</originalsourceid><addsrcrecordid>eNp1kUFrFTEQx4Mo9tl68AtIwEs9bJtJNm-zRyltFQqCtOeQ7E7alOzmmWQt79ub59YeBHMZkvnNjyF_Qj4AO4N6zktyZ9ACtK_IBqToGt738jXZMNZCAyD4EXmX8yNjjPcM3pIjrlroWtltyNOlcziUTKOjydvogvnlZ2rmkS6hpHqJAQsN_v6h0JLQlAnnQuNMd8EUPPTKQ4qTXTJ1MU2m-LiOvzznYqwPvuwPU0MMwdzjfELeOBMyvn-ux-Tu6vL24mtz8_3628WXm2YQSrVNywZut3zoR2alsxZkNyrZcbFFacAYBKaUMspaJRSK3rBxBLllHAeUauzFMTldvbsUfy6Yi558HrAuMWNcsgaleqEkF7yin_5BH-OS5rqdhh5ktbb8QH1eqSHFnBM6vUt-MmmvgelDGrqmof-kUdmPz8bFTji-kH-_vwLnK_DkA-7_b9K3P65W5W-yrZWM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1915560422</pqid></control><display><type>article</type><title>Effects of riboflavin and ultraviolet light treatment on platelet thrombus formation and thrombus stability on collagen</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Terada, Chikahiro ; Shiba, Masayuki ; Nagai, Tadashi ; Satake, Masahiro</creator><creatorcontrib>Terada, Chikahiro ; Shiba, Masayuki ; Nagai, Tadashi ; Satake, Masahiro</creatorcontrib><description>BACKGROUND
Pathogen reduction technologies (PRTs) are considered for the implementation of safer platelet (PLT) transfusion. PRT treatment involves the addition of a photosensitizer to a blood component followed by ultraviolet (UV) light irradiation. However, the effects of PRT treatment on PLT thrombus formation and thrombus stability have not been satisfactorily clarified.
STUDY DESIGN AND METHODS
Leukoreduced PLT concentrates (PCs) were treated with riboflavin and UV light (Mirasol PRT). PLT thrombus formation on collagen was evaluated by the microchannel method, by which the total amount of PLTs deposited was measured as indices of thrombus formation and thrombus stability. Using a cone‐plate shear‐induced PLT aggregometer, PLT reactivity in blood flow was examined in a wide range of shear stresses of 6 to 108 dyn/cm2.
RESULTS
There was no significant difference in surface coverage between PRT‐treated PLTs and control PLTs on collagen. On the other hand, the total amount of PRT‐treated PLTs deposited was higher than that of control PLTs. The promotive effect of PRT treatment on PLT deposition completely disappeared in the presence of tirofiban, a potent integrin αIIbβ3 inhibitor. The percentage of the dissociation of PRT‐treated PLTs on collagen was lower than that of control PLTs after flushing with phosphate‐buffered saline. PRT treatment significantly inhibited PLT aggregation under high‐shear‐stress conditions.
CONCLUSION
Riboflavin‐based PRT treatment of PCs leads to the enhancement of PLT thrombus formation and thrombus stability on collagen. However, it does not enhance the reactivity of PLTs not in contact with collagen under high‐shear‐stress conditions.</description><identifier>ISSN: 0041-1132</identifier><identifier>EISSN: 1537-2995</identifier><identifier>DOI: 10.1111/trf.14114</identifier><identifier>PMID: 28417457</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Agglomeration ; Blood clots ; Blood flow ; Blood Platelets - drug effects ; Blood Platelets - radiation effects ; Buffers ; Catheters ; Collagen ; Collagen - chemistry ; Contact stresses ; Flow stability ; Flushing ; Humans ; Inhibitors ; Irradiation ; Light irradiation ; Mechanical stimuli ; Pathogens ; Phosphate ; Phosphates ; Platelet Glycoprotein GPIIb-IIIa Complex - chemistry ; Platelets ; Riboflavin ; Riboflavin - pharmacology ; Shear ; Stress concentration ; Thrombosis ; Thrombosis - etiology ; Transfusion ; Ultraviolet radiation ; Ultraviolet Rays ; Vitamin B</subject><ispartof>Transfusion (Philadelphia, Pa.), 2017-07, Vol.57 (7), p.1772-1780</ispartof><rights>2017 AABB</rights><rights>2017 AABB.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3884-40c2b62c9d0b5fbb157d857236e5a1aae10888a8bb838e39a0dd15602ece58d93</citedby><cites>FETCH-LOGICAL-c3884-40c2b62c9d0b5fbb157d857236e5a1aae10888a8bb838e39a0dd15602ece58d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Ftrf.14114$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Ftrf.14114$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28417457$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Terada, Chikahiro</creatorcontrib><creatorcontrib>Shiba, Masayuki</creatorcontrib><creatorcontrib>Nagai, Tadashi</creatorcontrib><creatorcontrib>Satake, Masahiro</creatorcontrib><title>Effects of riboflavin and ultraviolet light treatment on platelet thrombus formation and thrombus stability on collagen</title><title>Transfusion (Philadelphia, Pa.)</title><addtitle>Transfusion</addtitle><description>BACKGROUND
Pathogen reduction technologies (PRTs) are considered for the implementation of safer platelet (PLT) transfusion. PRT treatment involves the addition of a photosensitizer to a blood component followed by ultraviolet (UV) light irradiation. However, the effects of PRT treatment on PLT thrombus formation and thrombus stability have not been satisfactorily clarified.
STUDY DESIGN AND METHODS
Leukoreduced PLT concentrates (PCs) were treated with riboflavin and UV light (Mirasol PRT). PLT thrombus formation on collagen was evaluated by the microchannel method, by which the total amount of PLTs deposited was measured as indices of thrombus formation and thrombus stability. Using a cone‐plate shear‐induced PLT aggregometer, PLT reactivity in blood flow was examined in a wide range of shear stresses of 6 to 108 dyn/cm2.
RESULTS
There was no significant difference in surface coverage between PRT‐treated PLTs and control PLTs on collagen. On the other hand, the total amount of PRT‐treated PLTs deposited was higher than that of control PLTs. The promotive effect of PRT treatment on PLT deposition completely disappeared in the presence of tirofiban, a potent integrin αIIbβ3 inhibitor. The percentage of the dissociation of PRT‐treated PLTs on collagen was lower than that of control PLTs after flushing with phosphate‐buffered saline. PRT treatment significantly inhibited PLT aggregation under high‐shear‐stress conditions.
CONCLUSION
Riboflavin‐based PRT treatment of PCs leads to the enhancement of PLT thrombus formation and thrombus stability on collagen. However, it does not enhance the reactivity of PLTs not in contact with collagen under high‐shear‐stress conditions.</description><subject>Agglomeration</subject><subject>Blood clots</subject><subject>Blood flow</subject><subject>Blood Platelets - drug effects</subject><subject>Blood Platelets - radiation effects</subject><subject>Buffers</subject><subject>Catheters</subject><subject>Collagen</subject><subject>Collagen - chemistry</subject><subject>Contact stresses</subject><subject>Flow stability</subject><subject>Flushing</subject><subject>Humans</subject><subject>Inhibitors</subject><subject>Irradiation</subject><subject>Light irradiation</subject><subject>Mechanical stimuli</subject><subject>Pathogens</subject><subject>Phosphate</subject><subject>Phosphates</subject><subject>Platelet Glycoprotein GPIIb-IIIa Complex - chemistry</subject><subject>Platelets</subject><subject>Riboflavin</subject><subject>Riboflavin - pharmacology</subject><subject>Shear</subject><subject>Stress concentration</subject><subject>Thrombosis</subject><subject>Thrombosis - etiology</subject><subject>Transfusion</subject><subject>Ultraviolet radiation</subject><subject>Ultraviolet Rays</subject><subject>Vitamin B</subject><issn>0041-1132</issn><issn>1537-2995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUFrFTEQx4Mo9tl68AtIwEs9bJtJNm-zRyltFQqCtOeQ7E7alOzmmWQt79ub59YeBHMZkvnNjyF_Qj4AO4N6zktyZ9ACtK_IBqToGt738jXZMNZCAyD4EXmX8yNjjPcM3pIjrlroWtltyNOlcziUTKOjydvogvnlZ2rmkS6hpHqJAQsN_v6h0JLQlAnnQuNMd8EUPPTKQ4qTXTJ1MU2m-LiOvzznYqwPvuwPU0MMwdzjfELeOBMyvn-ux-Tu6vL24mtz8_3628WXm2YQSrVNywZut3zoR2alsxZkNyrZcbFFacAYBKaUMspaJRSK3rBxBLllHAeUauzFMTldvbsUfy6Yi558HrAuMWNcsgaleqEkF7yin_5BH-OS5rqdhh5ktbb8QH1eqSHFnBM6vUt-MmmvgelDGrqmof-kUdmPz8bFTji-kH-_vwLnK_DkA-7_b9K3P65W5W-yrZWM</recordid><startdate>201707</startdate><enddate>201707</enddate><creator>Terada, Chikahiro</creator><creator>Shiba, Masayuki</creator><creator>Nagai, Tadashi</creator><creator>Satake, Masahiro</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201707</creationdate><title>Effects of riboflavin and ultraviolet light treatment on platelet thrombus formation and thrombus stability on collagen</title><author>Terada, Chikahiro ; Shiba, Masayuki ; Nagai, Tadashi ; Satake, Masahiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3884-40c2b62c9d0b5fbb157d857236e5a1aae10888a8bb838e39a0dd15602ece58d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Agglomeration</topic><topic>Blood clots</topic><topic>Blood flow</topic><topic>Blood Platelets - drug effects</topic><topic>Blood Platelets - radiation effects</topic><topic>Buffers</topic><topic>Catheters</topic><topic>Collagen</topic><topic>Collagen - chemistry</topic><topic>Contact stresses</topic><topic>Flow stability</topic><topic>Flushing</topic><topic>Humans</topic><topic>Inhibitors</topic><topic>Irradiation</topic><topic>Light irradiation</topic><topic>Mechanical stimuli</topic><topic>Pathogens</topic><topic>Phosphate</topic><topic>Phosphates</topic><topic>Platelet Glycoprotein GPIIb-IIIa Complex - chemistry</topic><topic>Platelets</topic><topic>Riboflavin</topic><topic>Riboflavin - pharmacology</topic><topic>Shear</topic><topic>Stress concentration</topic><topic>Thrombosis</topic><topic>Thrombosis - etiology</topic><topic>Transfusion</topic><topic>Ultraviolet radiation</topic><topic>Ultraviolet Rays</topic><topic>Vitamin B</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Terada, Chikahiro</creatorcontrib><creatorcontrib>Shiba, Masayuki</creatorcontrib><creatorcontrib>Nagai, Tadashi</creatorcontrib><creatorcontrib>Satake, Masahiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transfusion (Philadelphia, Pa.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Terada, Chikahiro</au><au>Shiba, Masayuki</au><au>Nagai, Tadashi</au><au>Satake, Masahiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of riboflavin and ultraviolet light treatment on platelet thrombus formation and thrombus stability on collagen</atitle><jtitle>Transfusion (Philadelphia, Pa.)</jtitle><addtitle>Transfusion</addtitle><date>2017-07</date><risdate>2017</risdate><volume>57</volume><issue>7</issue><spage>1772</spage><epage>1780</epage><pages>1772-1780</pages><issn>0041-1132</issn><eissn>1537-2995</eissn><abstract>BACKGROUND
Pathogen reduction technologies (PRTs) are considered for the implementation of safer platelet (PLT) transfusion. PRT treatment involves the addition of a photosensitizer to a blood component followed by ultraviolet (UV) light irradiation. However, the effects of PRT treatment on PLT thrombus formation and thrombus stability have not been satisfactorily clarified.
STUDY DESIGN AND METHODS
Leukoreduced PLT concentrates (PCs) were treated with riboflavin and UV light (Mirasol PRT). PLT thrombus formation on collagen was evaluated by the microchannel method, by which the total amount of PLTs deposited was measured as indices of thrombus formation and thrombus stability. Using a cone‐plate shear‐induced PLT aggregometer, PLT reactivity in blood flow was examined in a wide range of shear stresses of 6 to 108 dyn/cm2.
RESULTS
There was no significant difference in surface coverage between PRT‐treated PLTs and control PLTs on collagen. On the other hand, the total amount of PRT‐treated PLTs deposited was higher than that of control PLTs. The promotive effect of PRT treatment on PLT deposition completely disappeared in the presence of tirofiban, a potent integrin αIIbβ3 inhibitor. The percentage of the dissociation of PRT‐treated PLTs on collagen was lower than that of control PLTs after flushing with phosphate‐buffered saline. PRT treatment significantly inhibited PLT aggregation under high‐shear‐stress conditions.
CONCLUSION
Riboflavin‐based PRT treatment of PCs leads to the enhancement of PLT thrombus formation and thrombus stability on collagen. However, it does not enhance the reactivity of PLTs not in contact with collagen under high‐shear‐stress conditions.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>28417457</pmid><doi>10.1111/trf.14114</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Transfusion (Philadelphia, Pa.), 2017-07, Vol.57 (7), p.1772-1780 |
| issn | 0041-1132 1537-2995 |
| language | eng |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Agglomeration Blood clots Blood flow Blood Platelets - drug effects Blood Platelets - radiation effects Buffers Catheters Collagen Collagen - chemistry Contact stresses Flow stability Flushing Humans Inhibitors Irradiation Light irradiation Mechanical stimuli Pathogens Phosphate Phosphates Platelet Glycoprotein GPIIb-IIIa Complex - chemistry Platelets Riboflavin Riboflavin - pharmacology Shear Stress concentration Thrombosis Thrombosis - etiology Transfusion Ultraviolet radiation Ultraviolet Rays Vitamin B |
| title | Effects of riboflavin and ultraviolet light treatment on platelet thrombus formation and thrombus stability on collagen |
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