A yeast-based method for the detection of cyto and genotoxicity
A miniaturized short-term in vivo genotoxicity screening assay based on genetically modified yeast ( Saccharomyces cerevisiae) cells was performed to explore the capacity of this eukaryotic organism to detect the presence of genotoxic compounds. An increased general sensitivity of yeast cells to tox...
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| Veröffentlicht in: | Toxicology in vitro 2003-10, Vol.17 (5), p.709-716 |
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| creator | Lichtenberg-Fraté, Hella Schmitt, Marcel Gellert, Georg Ludwig, Jost |
| description | A miniaturized short-term in vivo genotoxicity screening assay based on genetically modified yeast (
Saccharomyces cerevisiae) cells was performed to explore the capacity of this eukaryotic organism to detect the presence of genotoxic compounds. An increased general sensitivity of yeast cells to toxic compounds was obtained by using a strain being deleted in the prominent pleiotropic drug resistance mediating efflux transporters PDR5, SNQ2 and YOR1. In order to detect genotoxic effects, a yeast optimized version of the green fluorescent protein (GFP) was fused to the
RAD54 promoter that is activated upon DNA damage. Various model substances including the oxygenated fuel additive methyl tertiary-butyl ether (MTBE) and the direct acting genotoxins methyl-
N-nitro-
N-nitrosoguanidine (MNNG) and 4-nitroquinoline-1-oxide (4-NQO) were tested. All model substances were in parallel examined for chronic cytotoxicity. The results point out the sufficiency of both the sensitivity of the yeast cells to detect chronic cytotoxicity and the intensity of the fluorescence signal for the assessment of genotoxic effects. Thus, the test enables simultaneous detection of cytotoxic and genotoxic effects. By partial automation and implementation of the test in the microtitre scale this bioassay allows parallel sensitive pre-screening of numerous samples. |
| doi_str_mv | 10.1016/S0887-2333(03)00129-2 |
| format | Article |
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Saccharomyces cerevisiae) cells was performed to explore the capacity of this eukaryotic organism to detect the presence of genotoxic compounds. An increased general sensitivity of yeast cells to toxic compounds was obtained by using a strain being deleted in the prominent pleiotropic drug resistance mediating efflux transporters PDR5, SNQ2 and YOR1. In order to detect genotoxic effects, a yeast optimized version of the green fluorescent protein (GFP) was fused to the
RAD54 promoter that is activated upon DNA damage. Various model substances including the oxygenated fuel additive methyl tertiary-butyl ether (MTBE) and the direct acting genotoxins methyl-
N-nitro-
N-nitrosoguanidine (MNNG) and 4-nitroquinoline-1-oxide (4-NQO) were tested. All model substances were in parallel examined for chronic cytotoxicity. The results point out the sufficiency of both the sensitivity of the yeast cells to detect chronic cytotoxicity and the intensity of the fluorescence signal for the assessment of genotoxic effects. Thus, the test enables simultaneous detection of cytotoxic and genotoxic effects. By partial automation and implementation of the test in the microtitre scale this bioassay allows parallel sensitive pre-screening of numerous samples.</description><identifier>ISSN: 0887-2333</identifier><identifier>EISSN: 1879-3177</identifier><identifier>DOI: 10.1016/S0887-2333(03)00129-2</identifier><identifier>PMID: 14599467</identifier><identifier>CODEN: TIVIEQ</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Biological and medical sciences ; Cell Division - drug effects ; Cell Division - physiology ; Cytotoxicity ; DNA Damage ; DNA Helicases ; DNA Repair Enzymes ; Dose-Response Relationship, Drug ; General aspects. Methods ; Genotoxicity ; GFP ; Green Fluorescent Proteins ; Luminescent Proteins - genetics ; Luminescent Proteins - metabolism ; Medical sciences ; Mutagenicity Tests ; Mutagens - toxicity ; RAD54 ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - drug effects ; Saccharomyces cerevisiae - physiology ; Saccharomyces cerevisiae Proteins - genetics ; Saccharomyces cerevisiae Proteins - metabolism ; Short term in vitro test ; Toxicology ; Xenobiotics - toxicity</subject><ispartof>Toxicology in vitro, 2003-10, Vol.17 (5), p.709-716</ispartof><rights>2003 Elsevier Ltd</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c488t-2f255404993228bdf5c6f63410c2f0eeccb817627cf632dd10968868a90cb50f3</citedby><cites>FETCH-LOGICAL-c488t-2f255404993228bdf5c6f63410c2f0eeccb817627cf632dd10968868a90cb50f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0887233303001292$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,3537,23909,23910,25118,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15338934$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14599467$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lichtenberg-Fraté, Hella</creatorcontrib><creatorcontrib>Schmitt, Marcel</creatorcontrib><creatorcontrib>Gellert, Georg</creatorcontrib><creatorcontrib>Ludwig, Jost</creatorcontrib><title>A yeast-based method for the detection of cyto and genotoxicity</title><title>Toxicology in vitro</title><addtitle>Toxicol In Vitro</addtitle><description>A miniaturized short-term in vivo genotoxicity screening assay based on genetically modified yeast (
Saccharomyces cerevisiae) cells was performed to explore the capacity of this eukaryotic organism to detect the presence of genotoxic compounds. An increased general sensitivity of yeast cells to toxic compounds was obtained by using a strain being deleted in the prominent pleiotropic drug resistance mediating efflux transporters PDR5, SNQ2 and YOR1. In order to detect genotoxic effects, a yeast optimized version of the green fluorescent protein (GFP) was fused to the
RAD54 promoter that is activated upon DNA damage. Various model substances including the oxygenated fuel additive methyl tertiary-butyl ether (MTBE) and the direct acting genotoxins methyl-
N-nitro-
N-nitrosoguanidine (MNNG) and 4-nitroquinoline-1-oxide (4-NQO) were tested. All model substances were in parallel examined for chronic cytotoxicity. The results point out the sufficiency of both the sensitivity of the yeast cells to detect chronic cytotoxicity and the intensity of the fluorescence signal for the assessment of genotoxic effects. Thus, the test enables simultaneous detection of cytotoxic and genotoxic effects. By partial automation and implementation of the test in the microtitre scale this bioassay allows parallel sensitive pre-screening of numerous samples.</description><subject>Biological and medical sciences</subject><subject>Cell Division - drug effects</subject><subject>Cell Division - physiology</subject><subject>Cytotoxicity</subject><subject>DNA Damage</subject><subject>DNA Helicases</subject><subject>DNA Repair Enzymes</subject><subject>Dose-Response Relationship, Drug</subject><subject>General aspects. Methods</subject><subject>Genotoxicity</subject><subject>GFP</subject><subject>Green Fluorescent Proteins</subject><subject>Luminescent Proteins - genetics</subject><subject>Luminescent Proteins - metabolism</subject><subject>Medical sciences</subject><subject>Mutagenicity Tests</subject><subject>Mutagens - toxicity</subject><subject>RAD54</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - drug effects</subject><subject>Saccharomyces cerevisiae - physiology</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>Short term in vitro test</subject><subject>Toxicology</subject><subject>Xenobiotics - toxicity</subject><issn>0887-2333</issn><issn>1879-3177</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtrGzEQgEVJqB23P6FBl5TksOlI2od0CiHkBYEc0p6FVho1KvYqkeSQ_fdd16Y-BgaGGb558BHyjcE5A9b-eAIpu4oLIU5BnAEwrir-icyZ7FQlWNcdkPl_ZEaOcv4DAI3k8JnMWN0oVbfdnFxc0hFNLlVvMjq6wvIcHfUx0fKM1GFBW0IcaPTUjiVSMzj6G4dY4nuwoYxfyKE3y4xfd3lBft1c_7y6qx4eb--vLh8qW0tZKu5509RQKyU4l73zjW19K2oGlntAtLaXrGt5Z6cud46BaqVspVFg-wa8WJDv270vKb6uMRe9CtnicmkGjOusmZRSScYmsNmCNsWcE3r9ksLKpFEz0Btz-p85vdGiYYqNualakOPdgXW_Qref2qmagJMdYLI1S5_MYEPec40QUol64i62HE463gImnW3AwaILaZKpXQwfvPIXIfGJCg</recordid><startdate>20031001</startdate><enddate>20031001</enddate><creator>Lichtenberg-Fraté, Hella</creator><creator>Schmitt, Marcel</creator><creator>Gellert, Georg</creator><creator>Ludwig, Jost</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20031001</creationdate><title>A yeast-based method for the detection of cyto and genotoxicity</title><author>Lichtenberg-Fraté, Hella ; Schmitt, Marcel ; Gellert, Georg ; Ludwig, Jost</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-2f255404993228bdf5c6f63410c2f0eeccb817627cf632dd10968868a90cb50f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Biological and medical sciences</topic><topic>Cell Division - drug effects</topic><topic>Cell Division - physiology</topic><topic>Cytotoxicity</topic><topic>DNA Damage</topic><topic>DNA Helicases</topic><topic>DNA Repair Enzymes</topic><topic>Dose-Response Relationship, Drug</topic><topic>General aspects. Methods</topic><topic>Genotoxicity</topic><topic>GFP</topic><topic>Green Fluorescent Proteins</topic><topic>Luminescent Proteins - genetics</topic><topic>Luminescent Proteins - metabolism</topic><topic>Medical sciences</topic><topic>Mutagenicity Tests</topic><topic>Mutagens - toxicity</topic><topic>RAD54</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - drug effects</topic><topic>Saccharomyces cerevisiae - physiology</topic><topic>Saccharomyces cerevisiae Proteins - genetics</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><topic>Short term in vitro test</topic><topic>Toxicology</topic><topic>Xenobiotics - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lichtenberg-Fraté, Hella</creatorcontrib><creatorcontrib>Schmitt, Marcel</creatorcontrib><creatorcontrib>Gellert, Georg</creatorcontrib><creatorcontrib>Ludwig, Jost</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Toxicology in vitro</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lichtenberg-Fraté, Hella</au><au>Schmitt, Marcel</au><au>Gellert, Georg</au><au>Ludwig, Jost</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A yeast-based method for the detection of cyto and genotoxicity</atitle><jtitle>Toxicology in vitro</jtitle><addtitle>Toxicol In Vitro</addtitle><date>2003-10-01</date><risdate>2003</risdate><volume>17</volume><issue>5</issue><spage>709</spage><epage>716</epage><pages>709-716</pages><issn>0887-2333</issn><eissn>1879-3177</eissn><coden>TIVIEQ</coden><abstract>A miniaturized short-term in vivo genotoxicity screening assay based on genetically modified yeast (
Saccharomyces cerevisiae) cells was performed to explore the capacity of this eukaryotic organism to detect the presence of genotoxic compounds. An increased general sensitivity of yeast cells to toxic compounds was obtained by using a strain being deleted in the prominent pleiotropic drug resistance mediating efflux transporters PDR5, SNQ2 and YOR1. In order to detect genotoxic effects, a yeast optimized version of the green fluorescent protein (GFP) was fused to the
RAD54 promoter that is activated upon DNA damage. Various model substances including the oxygenated fuel additive methyl tertiary-butyl ether (MTBE) and the direct acting genotoxins methyl-
N-nitro-
N-nitrosoguanidine (MNNG) and 4-nitroquinoline-1-oxide (4-NQO) were tested. All model substances were in parallel examined for chronic cytotoxicity. The results point out the sufficiency of both the sensitivity of the yeast cells to detect chronic cytotoxicity and the intensity of the fluorescence signal for the assessment of genotoxic effects. Thus, the test enables simultaneous detection of cytotoxic and genotoxic effects. By partial automation and implementation of the test in the microtitre scale this bioassay allows parallel sensitive pre-screening of numerous samples.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>14599467</pmid><doi>10.1016/S0887-2333(03)00129-2</doi><tpages>8</tpages></addata></record> |
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| subjects | Biological and medical sciences Cell Division - drug effects Cell Division - physiology Cytotoxicity DNA Damage DNA Helicases DNA Repair Enzymes Dose-Response Relationship, Drug General aspects. Methods Genotoxicity GFP Green Fluorescent Proteins Luminescent Proteins - genetics Luminescent Proteins - metabolism Medical sciences Mutagenicity Tests Mutagens - toxicity RAD54 Saccharomyces cerevisiae Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - physiology Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Short term in vitro test Toxicology Xenobiotics - toxicity |
| title | A yeast-based method for the detection of cyto and genotoxicity |
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