Chemical characterization of a novel polysaccharide ASKP-1 from Artemisia sphaerocephala Krasch seed and its macrophage activation via MAPK, PI3k/Akt and NF-κB signaling pathways in RAW264.7 cells
The aim of this study was to investigate the molecular mechanism underlying the immunomodulatory effect of the purified Artemisia sphaerocephala Krasch seed polysaccharide (ASKP-1) in RAW264.7 macrophages. Chemical characteristic analysis revealed that ASKP-1 consisted of 14.1% mannose, 56.9% glucos...
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| Veröffentlicht in: | Food & function 2017-03, Vol.8 (3), p.1299-1312 |
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| description | The aim of this study was to investigate the molecular mechanism underlying the immunomodulatory effect of the purified
Artemisia sphaerocephala
Krasch seed polysaccharide (ASKP-1) in RAW264.7 macrophages. Chemical characteristic analysis revealed that ASKP-1 consisted of 14.1% mannose, 56.9% glucose and 19.6% galactose with the average molecular weight of 9.08 × 10
5
Da and the mixed glycan backbone structure containing 1→4)-Glc
p
(39.8%), 1→6)-Gal
p
(18.8%), 1→3,6)-Man
p
(19.6%), 1→)-Glc
p
(10.8%), 2→6)-Man
p
(4.0%) and 2→3,5)-Ara
f
(7.0%).
In vitro
studies showed that ASKP-1 markedly induced the release of cytotoxic molecules (NO and ROS) and secretion of the cytokines (TNF-α, INF-β, and IL-6) and significantly enhanced the phagocytosis of RAW264.7 macrophages. Furthermore, TLR4 was found to be a recognized target of ASKP-1 and its related mitogen-activated protein (MAPK) and phosphoinositide 3-kinase (PI3K)/Akt, including phosphorylated ERK, JNK, p38 and Akt, were rapidly activated by ASKP-1 in RAW264.7 macrophages. Moreover, ASKP-1 was found to cause the nuclear translocation of the nuclear factor NF-κB subunit p65 and the degradation of IκB-α in RAW264.7 macrophages. All these findings suggest that MAPK, PI3K/Akt and NF-κB pathways are involved in ASKP-1-induced macrophage activation, and ASKP-1 is a potential immunomodulating function food.
The aim of this study was to investigate the molecular mechanism underlying the immunomodulatory effect of the purified
Artemisia sphaerocephala
Krasch seed polysaccharide (ASKP-1) in RAW264.7 macrophages. |
| doi_str_mv | 10.1039/c6fo01699e |
| format | Article |
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Artemisia sphaerocephala
Krasch seed polysaccharide (ASKP-1) in RAW264.7 macrophages. Chemical characteristic analysis revealed that ASKP-1 consisted of 14.1% mannose, 56.9% glucose and 19.6% galactose with the average molecular weight of 9.08 × 10
5
Da and the mixed glycan backbone structure containing 1→4)-Glc
p
(39.8%), 1→6)-Gal
p
(18.8%), 1→3,6)-Man
p
(19.6%), 1→)-Glc
p
(10.8%), 2→6)-Man
p
(4.0%) and 2→3,5)-Ara
f
(7.0%).
In vitro
studies showed that ASKP-1 markedly induced the release of cytotoxic molecules (NO and ROS) and secretion of the cytokines (TNF-α, INF-β, and IL-6) and significantly enhanced the phagocytosis of RAW264.7 macrophages. Furthermore, TLR4 was found to be a recognized target of ASKP-1 and its related mitogen-activated protein (MAPK) and phosphoinositide 3-kinase (PI3K)/Akt, including phosphorylated ERK, JNK, p38 and Akt, were rapidly activated by ASKP-1 in RAW264.7 macrophages. Moreover, ASKP-1 was found to cause the nuclear translocation of the nuclear factor NF-κB subunit p65 and the degradation of IκB-α in RAW264.7 macrophages. All these findings suggest that MAPK, PI3K/Akt and NF-κB pathways are involved in ASKP-1-induced macrophage activation, and ASKP-1 is a potential immunomodulating function food.
The aim of this study was to investigate the molecular mechanism underlying the immunomodulatory effect of the purified
Artemisia sphaerocephala
Krasch seed polysaccharide (ASKP-1) in RAW264.7 macrophages.</description><identifier>ISSN: 2042-6496</identifier><identifier>EISSN: 2042-650X</identifier><identifier>DOI: 10.1039/c6fo01699e</identifier><identifier>PMID: 28251195</identifier><language>eng</language><publisher>England</publisher><subject>Animals ; Artemisia ; Artemisia - chemistry ; Interleukin-6 - genetics ; Interleukin-6 - immunology ; Macrophage Activation - drug effects ; Macrophages - drug effects ; Macrophages - immunology ; Mice ; Mitogen-Activated Protein Kinase Kinases - genetics ; Mitogen-Activated Protein Kinase Kinases - immunology ; NF-kappa B - genetics ; NF-kappa B - immunology ; Phosphatidylinositol 3-Kinases - genetics ; Phosphatidylinositol 3-Kinases - immunology ; Plant Extracts - chemistry ; Plant Extracts - isolation & purification ; Plant Extracts - pharmacology ; Polysaccharides - chemistry ; Polysaccharides - isolation & purification ; Polysaccharides - pharmacology ; Proto-Oncogene Proteins c-akt - genetics ; Proto-Oncogene Proteins c-akt - immunology ; RAW 264.7 Cells ; Seeds - chemistry ; Signal Transduction ; Tumor Necrosis Factor-alpha - genetics ; Tumor Necrosis Factor-alpha - immunology</subject><ispartof>Food & function, 2017-03, Vol.8 (3), p.1299-1312</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-895e6b8645a96c1add1e06d063125bf8bd34c2bd676fe8f49f982eb6f677984d3</citedby><cites>FETCH-LOGICAL-c409t-895e6b8645a96c1add1e06d063125bf8bd34c2bd676fe8f49f982eb6f677984d3</cites><orcidid>0000-0002-8039-0525</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28251195$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ren, Daoyuan</creatorcontrib><creatorcontrib>Lin, Dehui</creatorcontrib><creatorcontrib>Alim, Aamina</creatorcontrib><creatorcontrib>Zheng, Quan</creatorcontrib><creatorcontrib>Yang, Xingbin</creatorcontrib><title>Chemical characterization of a novel polysaccharide ASKP-1 from Artemisia sphaerocephala Krasch seed and its macrophage activation via MAPK, PI3k/Akt and NF-κB signaling pathways in RAW264.7 cells</title><title>Food & function</title><addtitle>Food Funct</addtitle><description>The aim of this study was to investigate the molecular mechanism underlying the immunomodulatory effect of the purified
Artemisia sphaerocephala
Krasch seed polysaccharide (ASKP-1) in RAW264.7 macrophages. Chemical characteristic analysis revealed that ASKP-1 consisted of 14.1% mannose, 56.9% glucose and 19.6% galactose with the average molecular weight of 9.08 × 10
5
Da and the mixed glycan backbone structure containing 1→4)-Glc
p
(39.8%), 1→6)-Gal
p
(18.8%), 1→3,6)-Man
p
(19.6%), 1→)-Glc
p
(10.8%), 2→6)-Man
p
(4.0%) and 2→3,5)-Ara
f
(7.0%).
In vitro
studies showed that ASKP-1 markedly induced the release of cytotoxic molecules (NO and ROS) and secretion of the cytokines (TNF-α, INF-β, and IL-6) and significantly enhanced the phagocytosis of RAW264.7 macrophages. Furthermore, TLR4 was found to be a recognized target of ASKP-1 and its related mitogen-activated protein (MAPK) and phosphoinositide 3-kinase (PI3K)/Akt, including phosphorylated ERK, JNK, p38 and Akt, were rapidly activated by ASKP-1 in RAW264.7 macrophages. Moreover, ASKP-1 was found to cause the nuclear translocation of the nuclear factor NF-κB subunit p65 and the degradation of IκB-α in RAW264.7 macrophages. All these findings suggest that MAPK, PI3K/Akt and NF-κB pathways are involved in ASKP-1-induced macrophage activation, and ASKP-1 is a potential immunomodulating function food.
The aim of this study was to investigate the molecular mechanism underlying the immunomodulatory effect of the purified
Artemisia sphaerocephala
Krasch seed polysaccharide (ASKP-1) in RAW264.7 macrophages.</description><subject>Animals</subject><subject>Artemisia</subject><subject>Artemisia - chemistry</subject><subject>Interleukin-6 - genetics</subject><subject>Interleukin-6 - immunology</subject><subject>Macrophage Activation - drug effects</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - immunology</subject><subject>Mice</subject><subject>Mitogen-Activated Protein Kinase Kinases - genetics</subject><subject>Mitogen-Activated Protein Kinase Kinases - immunology</subject><subject>NF-kappa B - genetics</subject><subject>NF-kappa B - immunology</subject><subject>Phosphatidylinositol 3-Kinases - genetics</subject><subject>Phosphatidylinositol 3-Kinases - immunology</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - pharmacology</subject><subject>Polysaccharides - chemistry</subject><subject>Polysaccharides - isolation & purification</subject><subject>Polysaccharides - pharmacology</subject><subject>Proto-Oncogene Proteins c-akt - genetics</subject><subject>Proto-Oncogene Proteins c-akt - immunology</subject><subject>RAW 264.7 Cells</subject><subject>Seeds - chemistry</subject><subject>Signal Transduction</subject><subject>Tumor Necrosis Factor-alpha - genetics</subject><subject>Tumor Necrosis Factor-alpha - immunology</subject><issn>2042-6496</issn><issn>2042-650X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk1v1DAQhiMEolXphTtouCFEWttJHPsYVl2ottAVH4Jb5PhjY5rEwc4uWn4ad678JrzdttwQvoyleead8bxOkscYnWCU8VNJjUOYcq7vJYcE5SSlBfpy__aec3qQHIfwFcWTcc44e5gcEEYKjHlxmPyatbq3UnQgW-GFnLS3P8Rk3QDOgIDBbXQHo-u2QcgdYpWG6sNimWIw3vVQ-SkKBCsgjK3Q3kkdYydg4UWQLQStFYhBgZ0C9EJ6F9MrDbGV3ewbbWLx22q5eAnL8-zqtLqargvezdPfP19BsKtBdHZYwSim9rvYBrADvK8-E5qflCB114VHyQMjuqCPb-JR8ml-9nH2Jr24fH0-qy5SmSM-pYwXmjaM5oXgVGKhFNaIKkQzTIrGsEZluSSNoiU1mpmcG86IbqihZclZrrKj5Pled_Tu21qHqY5P300gBu3WocaMxQUjRvL_QMusJFmBaURf7NG4nBC8NvXobS_8tsao3plcz-j88trkswg_vdFdN71Wd-itpRF4tgd8kHfZv7-kHpWJzJN_Mdkfmty4eA</recordid><startdate>20170322</startdate><enddate>20170322</enddate><creator>Ren, Daoyuan</creator><creator>Lin, Dehui</creator><creator>Alim, Aamina</creator><creator>Zheng, Quan</creator><creator>Yang, Xingbin</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope><orcidid>https://orcid.org/0000-0002-8039-0525</orcidid></search><sort><creationdate>20170322</creationdate><title>Chemical characterization of a novel polysaccharide ASKP-1 from Artemisia sphaerocephala Krasch seed and its macrophage activation via MAPK, PI3k/Akt and NF-κB signaling pathways in RAW264.7 cells</title><author>Ren, Daoyuan ; Lin, Dehui ; Alim, Aamina ; Zheng, Quan ; Yang, Xingbin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-895e6b8645a96c1add1e06d063125bf8bd34c2bd676fe8f49f982eb6f677984d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Artemisia</topic><topic>Artemisia - chemistry</topic><topic>Interleukin-6 - genetics</topic><topic>Interleukin-6 - immunology</topic><topic>Macrophage Activation - drug effects</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - immunology</topic><topic>Mice</topic><topic>Mitogen-Activated Protein Kinase Kinases - genetics</topic><topic>Mitogen-Activated Protein Kinase Kinases - immunology</topic><topic>NF-kappa B - genetics</topic><topic>NF-kappa B - immunology</topic><topic>Phosphatidylinositol 3-Kinases - genetics</topic><topic>Phosphatidylinositol 3-Kinases - immunology</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - isolation & purification</topic><topic>Plant Extracts - pharmacology</topic><topic>Polysaccharides - chemistry</topic><topic>Polysaccharides - isolation & purification</topic><topic>Polysaccharides - pharmacology</topic><topic>Proto-Oncogene Proteins c-akt - genetics</topic><topic>Proto-Oncogene Proteins c-akt - immunology</topic><topic>RAW 264.7 Cells</topic><topic>Seeds - chemistry</topic><topic>Signal Transduction</topic><topic>Tumor Necrosis Factor-alpha - genetics</topic><topic>Tumor Necrosis Factor-alpha - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ren, Daoyuan</creatorcontrib><creatorcontrib>Lin, Dehui</creatorcontrib><creatorcontrib>Alim, Aamina</creatorcontrib><creatorcontrib>Zheng, Quan</creatorcontrib><creatorcontrib>Yang, Xingbin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Food & function</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ren, Daoyuan</au><au>Lin, Dehui</au><au>Alim, Aamina</au><au>Zheng, Quan</au><au>Yang, Xingbin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chemical characterization of a novel polysaccharide ASKP-1 from Artemisia sphaerocephala Krasch seed and its macrophage activation via MAPK, PI3k/Akt and NF-κB signaling pathways in RAW264.7 cells</atitle><jtitle>Food & function</jtitle><addtitle>Food Funct</addtitle><date>2017-03-22</date><risdate>2017</risdate><volume>8</volume><issue>3</issue><spage>1299</spage><epage>1312</epage><pages>1299-1312</pages><issn>2042-6496</issn><eissn>2042-650X</eissn><abstract>The aim of this study was to investigate the molecular mechanism underlying the immunomodulatory effect of the purified
Artemisia sphaerocephala
Krasch seed polysaccharide (ASKP-1) in RAW264.7 macrophages. Chemical characteristic analysis revealed that ASKP-1 consisted of 14.1% mannose, 56.9% glucose and 19.6% galactose with the average molecular weight of 9.08 × 10
5
Da and the mixed glycan backbone structure containing 1→4)-Glc
p
(39.8%), 1→6)-Gal
p
(18.8%), 1→3,6)-Man
p
(19.6%), 1→)-Glc
p
(10.8%), 2→6)-Man
p
(4.0%) and 2→3,5)-Ara
f
(7.0%).
In vitro
studies showed that ASKP-1 markedly induced the release of cytotoxic molecules (NO and ROS) and secretion of the cytokines (TNF-α, INF-β, and IL-6) and significantly enhanced the phagocytosis of RAW264.7 macrophages. Furthermore, TLR4 was found to be a recognized target of ASKP-1 and its related mitogen-activated protein (MAPK) and phosphoinositide 3-kinase (PI3K)/Akt, including phosphorylated ERK, JNK, p38 and Akt, were rapidly activated by ASKP-1 in RAW264.7 macrophages. Moreover, ASKP-1 was found to cause the nuclear translocation of the nuclear factor NF-κB subunit p65 and the degradation of IκB-α in RAW264.7 macrophages. All these findings suggest that MAPK, PI3K/Akt and NF-κB pathways are involved in ASKP-1-induced macrophage activation, and ASKP-1 is a potential immunomodulating function food.
The aim of this study was to investigate the molecular mechanism underlying the immunomodulatory effect of the purified
Artemisia sphaerocephala
Krasch seed polysaccharide (ASKP-1) in RAW264.7 macrophages.</abstract><cop>England</cop><pmid>28251195</pmid><doi>10.1039/c6fo01699e</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-8039-0525</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2042-6496 |
| ispartof | Food & function, 2017-03, Vol.8 (3), p.1299-1312 |
| issn | 2042-6496 2042-650X |
| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008- |
| subjects | Animals Artemisia Artemisia - chemistry Interleukin-6 - genetics Interleukin-6 - immunology Macrophage Activation - drug effects Macrophages - drug effects Macrophages - immunology Mice Mitogen-Activated Protein Kinase Kinases - genetics Mitogen-Activated Protein Kinase Kinases - immunology NF-kappa B - genetics NF-kappa B - immunology Phosphatidylinositol 3-Kinases - genetics Phosphatidylinositol 3-Kinases - immunology Plant Extracts - chemistry Plant Extracts - isolation & purification Plant Extracts - pharmacology Polysaccharides - chemistry Polysaccharides - isolation & purification Polysaccharides - pharmacology Proto-Oncogene Proteins c-akt - genetics Proto-Oncogene Proteins c-akt - immunology RAW 264.7 Cells Seeds - chemistry Signal Transduction Tumor Necrosis Factor-alpha - genetics Tumor Necrosis Factor-alpha - immunology |
| title | Chemical characterization of a novel polysaccharide ASKP-1 from Artemisia sphaerocephala Krasch seed and its macrophage activation via MAPK, PI3k/Akt and NF-κB signaling pathways in RAW264.7 cells |
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