Combination of imaging flow cytometry and time‐lapse microscopy for the study of label‐free morphology dynamics of hematopoietic cells

Cell differentiation is a longitudinal and dynamic process. Studying and quantifying such a process require tools combining precise time resolution and statistical power. Imaging flow cytometry (IFC) provides statistically significant number of microscopy images of individual cells in a sample at a...

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Veröffentlicht in:	Cytometry A 2017-03, Vol.91 (3), p.254-260
Hauptverfasser:	Cosette, Jérémie, Moussy, Alice, Paldi, Andras, Stockholm, Daniel
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens, CD34 - isolation & purification Antigens, CD34 - metabolism Biological activity Blood CD34 antigen Cell Count - methods Cell differentiation Cell Differentiation - genetics Cell morphology Cytology Cytometry Differentiation (biology) Fetal Blood - cytology Fetal Blood - metabolism Flow cytometry Flow Cytometry - methods Humans Image processing Image Processing, Computer-Assisted - methods Image segmentation imaging imaging flow cytometry Life Sciences Microscopy Microscopy - methods Morphology Roundness Statistical analysis Statistics Stimulation Time-Lapse Imaging - methods time‐lapse
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