The oral microbiome community variations associated with normal, potentially malignant disorders and malignant lesions of the oral cavity

The human oral microbiome has been known to show strong association with various oral diseases including oral cancer. This study attempts to characterize the community variations between normal, oral potentially malignant disorders (OPMD) and cancer associated microbiota using 16S rDNA sequencing. S...

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Veröffentlicht in:Malaysian journal of pathology 2017-04, Vol.39 (1), p.1-15
Hauptverfasser: Mok, S F, Karuthan, C, Cheah, Y K, Ngeow, W C, Rosnah, Z, Yap, S F, Ong, H K A
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container_issue 1
container_start_page 1
container_title Malaysian journal of pathology
container_volume 39
creator Mok, S F
Karuthan, C
Cheah, Y K
Ngeow, W C
Rosnah, Z
Yap, S F
Ong, H K A
description The human oral microbiome has been known to show strong association with various oral diseases including oral cancer. This study attempts to characterize the community variations between normal, oral potentially malignant disorders (OPMD) and cancer associated microbiota using 16S rDNA sequencing. Swab samples were collected from three groups (normal, OPMD and oral cancer) with nine subjects from each group. Bacteria genomic DNA was isolated in which full length 16S rDNA were amplified and used for cloned library sequencing. 16S rDNA sequences were processed and analysed with MOTHUR. A core oral microbiome was identified consisting of Firmicutes, Proteobacteria, Fusobacteria, Bacteroidetes and Actinobacteria at the phylum level while Streptococcus, Veillonella, Gemella, Granulicatella, Neisseria, Haemophilus, Selenomonas, Fusobacterium, Leptotrichia, Prevotella, Porphyromonas and Lachnoanaerobaculum were detected at the genus level. Firmicutes and Streptococcus were the predominant phylum and genus respectively. Potential oral microbiome memberships unique to normal, OPMD and oral cancer oral cavities were also identified. Analysis of Molecular Variance (AMOVA) showed a significant difference between the normal and the cancer associated oral microbiota but not between the OPMD and the other two groups. However, 2D NMDS showed an overlapping of the OPMD associated oral microbiome between the normal and cancer groups. These findings indicated that oral microbes could be potential biomarkers to distinguish between normal, OPMD and cancer subjects.
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source MEDLINE; EZB-FREE-00999 freely available EZB journals
subjects Adult
Aged
Bacteria
Bacteria - pathogenicity
Biomarkers
Biomarkers, Tumor - analysis
Cloning
Dentistry
Female
Genomes
Head & neck cancer
Health risk assessment
HIV
Human immunodeficiency virus
Humans
Male
Medical prognosis
Microbiota - drug effects
Middle Aged
Mouth - microbiology
Mouth - pathology
Mouth Neoplasms - microbiology
Neoplasms - microbiology
Older people
Oral cancer
Oral hygiene
Patients
Skin cancer
Tumors
title The oral microbiome community variations associated with normal, potentially malignant disorders and malignant lesions of the oral cavity
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