The oral microbiome community variations associated with normal, potentially malignant disorders and malignant lesions of the oral cavity
The human oral microbiome has been known to show strong association with various oral diseases including oral cancer. This study attempts to characterize the community variations between normal, oral potentially malignant disorders (OPMD) and cancer associated microbiota using 16S rDNA sequencing. S...
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Veröffentlicht in: | Malaysian journal of pathology 2017-04, Vol.39 (1), p.1-15 |
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description | The human oral microbiome has been known to show strong association with various oral diseases including oral cancer. This study attempts to characterize the community variations between normal, oral potentially malignant disorders (OPMD) and cancer associated microbiota using 16S rDNA sequencing. Swab samples were collected from three groups (normal, OPMD and oral cancer) with nine subjects from each group. Bacteria genomic DNA was isolated in which full length 16S rDNA were amplified and used for cloned library sequencing. 16S rDNA sequences were processed and analysed with MOTHUR. A core oral microbiome was identified consisting of Firmicutes, Proteobacteria, Fusobacteria, Bacteroidetes and Actinobacteria at the phylum level while Streptococcus, Veillonella, Gemella, Granulicatella, Neisseria, Haemophilus, Selenomonas, Fusobacterium, Leptotrichia, Prevotella, Porphyromonas and Lachnoanaerobaculum were detected at the genus level. Firmicutes and Streptococcus were the predominant phylum and genus respectively. Potential oral microbiome memberships unique to normal, OPMD and oral cancer oral cavities were also identified. Analysis of Molecular Variance (AMOVA) showed a significant difference between the normal and the cancer associated oral microbiota but not between the OPMD and the other two groups. However, 2D NMDS showed an overlapping of the OPMD associated oral microbiome between the normal and cancer groups. These findings indicated that oral microbes could be potential biomarkers to distinguish between normal, OPMD and cancer subjects. |
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This study attempts to characterize the community variations between normal, oral potentially malignant disorders (OPMD) and cancer associated microbiota using 16S rDNA sequencing. Swab samples were collected from three groups (normal, OPMD and oral cancer) with nine subjects from each group. Bacteria genomic DNA was isolated in which full length 16S rDNA were amplified and used for cloned library sequencing. 16S rDNA sequences were processed and analysed with MOTHUR. A core oral microbiome was identified consisting of Firmicutes, Proteobacteria, Fusobacteria, Bacteroidetes and Actinobacteria at the phylum level while Streptococcus, Veillonella, Gemella, Granulicatella, Neisseria, Haemophilus, Selenomonas, Fusobacterium, Leptotrichia, Prevotella, Porphyromonas and Lachnoanaerobaculum were detected at the genus level. Firmicutes and Streptococcus were the predominant phylum and genus respectively. Potential oral microbiome memberships unique to normal, OPMD and oral cancer oral cavities were also identified. Analysis of Molecular Variance (AMOVA) showed a significant difference between the normal and the cancer associated oral microbiota but not between the OPMD and the other two groups. However, 2D NMDS showed an overlapping of the OPMD associated oral microbiome between the normal and cancer groups. These findings indicated that oral microbes could be potential biomarkers to distinguish between normal, OPMD and cancer subjects.</description><identifier>ISSN: 0126-8635</identifier><identifier>PMID: 28413200</identifier><language>eng</language><publisher>Malaysia: College of Pathologists, Academy of Medicine of Malaysia</publisher><subject>Adult ; Aged ; Bacteria ; Bacteria - pathogenicity ; Biomarkers ; Biomarkers, Tumor - analysis ; Cloning ; Dentistry ; Female ; Genomes ; Head & neck cancer ; Health risk assessment ; HIV ; Human immunodeficiency virus ; Humans ; Male ; Medical prognosis ; Microbiota - drug effects ; Middle Aged ; Mouth - microbiology ; Mouth - pathology ; Mouth Neoplasms - microbiology ; Neoplasms - microbiology ; Older people ; Oral cancer ; Oral hygiene ; Patients ; Skin cancer ; Tumors</subject><ispartof>Malaysian journal of pathology, 2017-04, Vol.39 (1), p.1-15</ispartof><rights>Copyright College of Pathologists, Academy of Medicine of Malaysia Apr 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28413200$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mok, S F</creatorcontrib><creatorcontrib>Karuthan, C</creatorcontrib><creatorcontrib>Cheah, Y K</creatorcontrib><creatorcontrib>Ngeow, W C</creatorcontrib><creatorcontrib>Rosnah, Z</creatorcontrib><creatorcontrib>Yap, S F</creatorcontrib><creatorcontrib>Ong, H K A</creatorcontrib><title>The oral microbiome community variations associated with normal, potentially malignant disorders and malignant lesions of the oral cavity</title><title>Malaysian journal of pathology</title><addtitle>Malays J Pathol</addtitle><description>The human oral microbiome has been known to show strong association with various oral diseases including oral cancer. This study attempts to characterize the community variations between normal, oral potentially malignant disorders (OPMD) and cancer associated microbiota using 16S rDNA sequencing. Swab samples were collected from three groups (normal, OPMD and oral cancer) with nine subjects from each group. Bacteria genomic DNA was isolated in which full length 16S rDNA were amplified and used for cloned library sequencing. 16S rDNA sequences were processed and analysed with MOTHUR. A core oral microbiome was identified consisting of Firmicutes, Proteobacteria, Fusobacteria, Bacteroidetes and Actinobacteria at the phylum level while Streptococcus, Veillonella, Gemella, Granulicatella, Neisseria, Haemophilus, Selenomonas, Fusobacterium, Leptotrichia, Prevotella, Porphyromonas and Lachnoanaerobaculum were detected at the genus level. Firmicutes and Streptococcus were the predominant phylum and genus respectively. Potential oral microbiome memberships unique to normal, OPMD and oral cancer oral cavities were also identified. Analysis of Molecular Variance (AMOVA) showed a significant difference between the normal and the cancer associated oral microbiota but not between the OPMD and the other two groups. However, 2D NMDS showed an overlapping of the OPMD associated oral microbiome between the normal and cancer groups. These findings indicated that oral microbes could be potential biomarkers to distinguish between normal, OPMD and cancer subjects.</description><subject>Adult</subject><subject>Aged</subject><subject>Bacteria</subject><subject>Bacteria - pathogenicity</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Cloning</subject><subject>Dentistry</subject><subject>Female</subject><subject>Genomes</subject><subject>Head & neck cancer</subject><subject>Health risk assessment</subject><subject>HIV</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Male</subject><subject>Medical prognosis</subject><subject>Microbiota - drug effects</subject><subject>Middle Aged</subject><subject>Mouth - microbiology</subject><subject>Mouth - pathology</subject><subject>Mouth Neoplasms - microbiology</subject><subject>Neoplasms - microbiology</subject><subject>Older people</subject><subject>Oral cancer</subject><subject>Oral hygiene</subject><subject>Patients</subject><subject>Skin cancer</subject><subject>Tumors</subject><issn>0126-8635</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpdkE1LAzEQhveg2Fr9CxLw4sFCkt3sJkcpfkHBSz0v2eysTcnHmmQr_Qn-a4NWEC8zzMsz7zvMSTHHhNZLXpdsVpzHuMO4roTgZ8WM8oqUFON58bnZAvJBGmS1Cr7T3gJS3trJ6XRAexm0TNq7iGSMXuUBevSh0xY5H6w0t2j0CVzS0pgDyoJ-c9Il1OvoQw8h77n-j24gfrv5AaXfZCX3OeuiOB2kiXB57Ivi9eF-s3parl8en1d36-VIS5GWQKta9ZxTAFJ3hClaUypE0w20w0xUVKmOKgysU6wcSF01WFHWU8YHRnItF8XNj-8Y_PsEMbVWRwXGSAd-ii3hnAvWNIxk9PofuvNTcPm6lghCclrJq0xdHamps9C3Y9BWhkP7--TyC34OeZ4</recordid><startdate>201704</startdate><enddate>201704</enddate><creator>Mok, S F</creator><creator>Karuthan, C</creator><creator>Cheah, Y K</creator><creator>Ngeow, W C</creator><creator>Rosnah, Z</creator><creator>Yap, S F</creator><creator>Ong, H K A</creator><general>College of Pathologists, Academy of Medicine of Malaysia</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BVBZV</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>201704</creationdate><title>The oral microbiome community variations associated with normal, potentially malignant disorders and malignant lesions of the oral cavity</title><author>Mok, S F ; Karuthan, C ; Cheah, Y K ; Ngeow, W C ; Rosnah, Z ; Yap, S F ; Ong, H K A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p239t-e246cd882ee16b15c2622997bf2b05942ccb2c0e5bc53f16470c25d258f512583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Bacteria</topic><topic>Bacteria - pathogenicity</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Cloning</topic><topic>Dentistry</topic><topic>Female</topic><topic>Genomes</topic><topic>Head & neck cancer</topic><topic>Health risk assessment</topic><topic>HIV</topic><topic>Human immunodeficiency virus</topic><topic>Humans</topic><topic>Male</topic><topic>Medical prognosis</topic><topic>Microbiota - drug effects</topic><topic>Middle Aged</topic><topic>Mouth - microbiology</topic><topic>Mouth - pathology</topic><topic>Mouth Neoplasms - microbiology</topic><topic>Neoplasms - microbiology</topic><topic>Older people</topic><topic>Oral cancer</topic><topic>Oral hygiene</topic><topic>Patients</topic><topic>Skin cancer</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mok, S F</creatorcontrib><creatorcontrib>Karuthan, C</creatorcontrib><creatorcontrib>Cheah, Y K</creatorcontrib><creatorcontrib>Ngeow, W C</creatorcontrib><creatorcontrib>Rosnah, Z</creatorcontrib><creatorcontrib>Yap, S F</creatorcontrib><creatorcontrib>Ong, H K A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>East & South Asia Database</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Malaysian journal of pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mok, S F</au><au>Karuthan, C</au><au>Cheah, Y K</au><au>Ngeow, W C</au><au>Rosnah, Z</au><au>Yap, S F</au><au>Ong, H K A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The oral microbiome community variations associated with normal, potentially malignant disorders and malignant lesions of the oral cavity</atitle><jtitle>Malaysian journal of pathology</jtitle><addtitle>Malays J Pathol</addtitle><date>2017-04</date><risdate>2017</risdate><volume>39</volume><issue>1</issue><spage>1</spage><epage>15</epage><pages>1-15</pages><issn>0126-8635</issn><abstract>The human oral microbiome has been known to show strong association with various oral diseases including oral cancer. This study attempts to characterize the community variations between normal, oral potentially malignant disorders (OPMD) and cancer associated microbiota using 16S rDNA sequencing. Swab samples were collected from three groups (normal, OPMD and oral cancer) with nine subjects from each group. Bacteria genomic DNA was isolated in which full length 16S rDNA were amplified and used for cloned library sequencing. 16S rDNA sequences were processed and analysed with MOTHUR. A core oral microbiome was identified consisting of Firmicutes, Proteobacteria, Fusobacteria, Bacteroidetes and Actinobacteria at the phylum level while Streptococcus, Veillonella, Gemella, Granulicatella, Neisseria, Haemophilus, Selenomonas, Fusobacterium, Leptotrichia, Prevotella, Porphyromonas and Lachnoanaerobaculum were detected at the genus level. Firmicutes and Streptococcus were the predominant phylum and genus respectively. Potential oral microbiome memberships unique to normal, OPMD and oral cancer oral cavities were also identified. Analysis of Molecular Variance (AMOVA) showed a significant difference between the normal and the cancer associated oral microbiota but not between the OPMD and the other two groups. However, 2D NMDS showed an overlapping of the OPMD associated oral microbiome between the normal and cancer groups. These findings indicated that oral microbes could be potential biomarkers to distinguish between normal, OPMD and cancer subjects.</abstract><cop>Malaysia</cop><pub>College of Pathologists, Academy of Medicine of Malaysia</pub><pmid>28413200</pmid><tpages>15</tpages></addata></record> |
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subjects | Adult Aged Bacteria Bacteria - pathogenicity Biomarkers Biomarkers, Tumor - analysis Cloning Dentistry Female Genomes Head & neck cancer Health risk assessment HIV Human immunodeficiency virus Humans Male Medical prognosis Microbiota - drug effects Middle Aged Mouth - microbiology Mouth - pathology Mouth Neoplasms - microbiology Neoplasms - microbiology Older people Oral cancer Oral hygiene Patients Skin cancer Tumors |
title | The oral microbiome community variations associated with normal, potentially malignant disorders and malignant lesions of the oral cavity |
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