Growth kinetics and hydrolytic enzyme production of Pseudomonas spp. isolated from pasteurized milk
Psychrotrophs, particularly Pseudomonas spp. are known to be the main determinants of the shelf-life of pasteurized milk and refrigerated raw milk. It is presumed that they mainly cause spoilage through the elaboration of proteinase and lipase enzymes. At the time of this research, under the relevan...
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| Veröffentlicht in: | Journal of dairy research 2003-08, Vol.70 (3), p.293-296 |
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| creator | Stevenson, R Gregory Rowe, Michael T Wisdom, G Brian Kilpatrick, David |
| description | Psychrotrophs, particularly Pseudomonas spp. are known to be the main determinants of the shelf-life of pasteurized milk and refrigerated raw milk. It is presumed that they mainly cause spoilage through the elaboration of proteinase and lipase enzymes. At the time of this research, under the relevant European Directive, one of the means of determining the quality of pasteurized milk was the pre-incubated count, which involves incubating the milk sample for 5 d at 6°C followed by a plate count. Examination of numerous pre-incubated counts revealed a bimodal rather than a normal distribution indicating that the types of contaminants in pasteurized milk may be as important as their initial concentration. Pseudomonads that gave particularly high (>5×106 cfu/ml) and low ( |
| doi_str_mv | 10.1017/S0022029903006204 |
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It is presumed that they mainly cause spoilage through the elaboration of proteinase and lipase enzymes. At the time of this research, under the relevant European Directive, one of the means of determining the quality of pasteurized milk was the pre-incubated count, which involves incubating the milk sample for 5 d at 6°C followed by a plate count. Examination of numerous pre-incubated counts revealed a bimodal rather than a normal distribution indicating that the types of contaminants in pasteurized milk may be as important as their initial concentration. Pseudomonads that gave particularly high (>5×106 cfu/ml) and low (<103 cfu/ml) pre-incubated counts were isolated (high and low count isolates respectively). After the organisms had been subjected to a cold shock no consistent trend between the groups of isolates was detected with respect to lag phase duration. However, the high count isolates consistently had a faster exponential growth rate. Unexpectedly, with the exception of one isolate, the low count isolates produced detectable proteinase and lipase earlier. In addition, with one exception, maximal proteinase and lipase production was observed with the low count isolates. These findings indicate that there is no causal relationship between selective growth advantage and ability to produce proteinase and lipase. It also indicates that the spoilage of pasteurized milk is a complex phenomenon and is worthy of further research.</description><identifier>ISSN: 0022-0299</identifier><identifier>EISSN: 1469-7629</identifier><identifier>DOI: 10.1017/S0022029903006204</identifier><identifier>PMID: 12916824</identifier><identifier>CODEN: JDRSAN</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Animals ; Biological and medical sciences ; Cattle - physiology ; cold stress ; Colony Count, Microbial - veterinary ; Contaminants ; Endopeptidases - metabolism ; Female ; Food Handling - methods ; Food industries ; Food Microbiology ; Food Preservation - methods ; Fundamental and applied biological sciences. Psychology ; Growth kinetics ; Kinetics ; Lipase - metabolism ; Milk ; Milk - microbiology ; Milk and cheese industries. Ice creams ; Pasteurization ; pasteurized milk ; plate count ; pollutants ; proteinases ; Pseudomonas ; Pseudomonas - enzymology ; Pseudomonas - growth & development ; Psychrotrophs ; raw milk ; shelf life ; spoilage ; triacylglycerol lipase</subject><ispartof>Journal of dairy research, 2003-08, Vol.70 (3), p.293-296</ispartof><rights>Proprietors of Journal of Dairy Research 2003</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-6a71b859e802f7f4c0b9eee5bf350fb73436c64c9513957325b3edfa7020f2dc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0022029903006204/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,778,782,27911,27912,55615</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14999313$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12916824$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stevenson, R Gregory</creatorcontrib><creatorcontrib>Rowe, Michael T</creatorcontrib><creatorcontrib>Wisdom, G Brian</creatorcontrib><creatorcontrib>Kilpatrick, David</creatorcontrib><title>Growth kinetics and hydrolytic enzyme production of Pseudomonas spp. isolated from pasteurized milk</title><title>Journal of dairy research</title><addtitle>Journal of Dairy Research</addtitle><description>Psychrotrophs, particularly Pseudomonas spp. are known to be the main determinants of the shelf-life of pasteurized milk and refrigerated raw milk. It is presumed that they mainly cause spoilage through the elaboration of proteinase and lipase enzymes. At the time of this research, under the relevant European Directive, one of the means of determining the quality of pasteurized milk was the pre-incubated count, which involves incubating the milk sample for 5 d at 6°C followed by a plate count. Examination of numerous pre-incubated counts revealed a bimodal rather than a normal distribution indicating that the types of contaminants in pasteurized milk may be as important as their initial concentration. Pseudomonads that gave particularly high (>5×106 cfu/ml) and low (<103 cfu/ml) pre-incubated counts were isolated (high and low count isolates respectively). After the organisms had been subjected to a cold shock no consistent trend between the groups of isolates was detected with respect to lag phase duration. However, the high count isolates consistently had a faster exponential growth rate. Unexpectedly, with the exception of one isolate, the low count isolates produced detectable proteinase and lipase earlier. In addition, with one exception, maximal proteinase and lipase production was observed with the low count isolates. These findings indicate that there is no causal relationship between selective growth advantage and ability to produce proteinase and lipase. It also indicates that the spoilage of pasteurized milk is a complex phenomenon and is worthy of further research.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle - physiology</subject><subject>cold stress</subject><subject>Colony Count, Microbial - veterinary</subject><subject>Contaminants</subject><subject>Endopeptidases - metabolism</subject><subject>Female</subject><subject>Food Handling - methods</subject><subject>Food industries</subject><subject>Food Microbiology</subject><subject>Food Preservation - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Growth kinetics</subject><subject>Kinetics</subject><subject>Lipase - metabolism</subject><subject>Milk</subject><subject>Milk - microbiology</subject><subject>Milk and cheese industries. Ice creams</subject><subject>Pasteurization</subject><subject>pasteurized milk</subject><subject>plate count</subject><subject>pollutants</subject><subject>proteinases</subject><subject>Pseudomonas</subject><subject>Pseudomonas - enzymology</subject><subject>Pseudomonas - growth & development</subject><subject>Psychrotrophs</subject><subject>raw milk</subject><subject>shelf life</subject><subject>spoilage</subject><subject>triacylglycerol lipase</subject><issn>0022-0299</issn><issn>1469-7629</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kV2P1CAYhYnRuOPqD_BGiYnedX2BFsqlmeho3Pi17t4SSmGXnbaM0EZnf7000ziJxisC53k_zgGhpwTOCBDx-gKAUqBSAgPgFMp7aEVKLgvBqbyPVrNczPoJepTSLQBhIPlDdEKoJLym5QqZTQw_xxu89YMdvUlYDy2-2bcxdPt8x3a42_cW72JoJzP6MODg8Jdkpzb0YdAJp93uDPsUOj3aFrsYerzTabRT9Hf5offd9jF64HSX7JPlPEWX795-X78vzj9vPqzfnBemlGQsuBakqStpa6BOuNJAI621VeNYBa4RrGTc8NLIijBZCUarhtnWaQEUHG0NO0WvDn3ztj8mm0bV-2Rs1-nBhikpUtc1BYAMvvgLvA1THPJuilIhJa0rliFygEwMKUXr1C76Xse9IqDm-NU_8eeaZ0vjqelte6xY8s7AywXQyejORT0Yn45cKaVkZB5eHDifo_z1R9dxq7hgolJ881Wtr9i3zcerT2p29PzAOx2Uvo655-UFBVLmP6eE83kyW-zovom-vbZH0_839BtVArSW</recordid><startdate>20030801</startdate><enddate>20030801</enddate><creator>Stevenson, R Gregory</creator><creator>Rowe, Michael T</creator><creator>Wisdom, G Brian</creator><creator>Kilpatrick, David</creator><general>Cambridge University Press</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7S</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope></search><sort><creationdate>20030801</creationdate><title>Growth kinetics and hydrolytic enzyme production of Pseudomonas spp. isolated from pasteurized milk</title><author>Stevenson, R Gregory ; Rowe, Michael T ; Wisdom, G Brian ; Kilpatrick, David</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-6a71b859e802f7f4c0b9eee5bf350fb73436c64c9513957325b3edfa7020f2dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cattle - physiology</topic><topic>cold stress</topic><topic>Colony Count, Microbial - veterinary</topic><topic>Contaminants</topic><topic>Endopeptidases - metabolism</topic><topic>Female</topic><topic>Food Handling - methods</topic><topic>Food industries</topic><topic>Food Microbiology</topic><topic>Food Preservation - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Growth kinetics</topic><topic>Kinetics</topic><topic>Lipase - metabolism</topic><topic>Milk</topic><topic>Milk - microbiology</topic><topic>Milk and cheese industries. Ice creams</topic><topic>Pasteurization</topic><topic>pasteurized milk</topic><topic>plate count</topic><topic>pollutants</topic><topic>proteinases</topic><topic>Pseudomonas</topic><topic>Pseudomonas - enzymology</topic><topic>Pseudomonas - growth & development</topic><topic>Psychrotrophs</topic><topic>raw milk</topic><topic>shelf life</topic><topic>spoilage</topic><topic>triacylglycerol lipase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stevenson, R Gregory</creatorcontrib><creatorcontrib>Rowe, Michael T</creatorcontrib><creatorcontrib>Wisdom, G Brian</creatorcontrib><creatorcontrib>Kilpatrick, David</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><jtitle>Journal of dairy research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stevenson, R Gregory</au><au>Rowe, Michael T</au><au>Wisdom, G Brian</au><au>Kilpatrick, David</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Growth kinetics and hydrolytic enzyme production of Pseudomonas spp. isolated from pasteurized milk</atitle><jtitle>Journal of dairy research</jtitle><addtitle>Journal of Dairy Research</addtitle><date>2003-08-01</date><risdate>2003</risdate><volume>70</volume><issue>3</issue><spage>293</spage><epage>296</epage><pages>293-296</pages><issn>0022-0299</issn><eissn>1469-7629</eissn><coden>JDRSAN</coden><abstract>Psychrotrophs, particularly Pseudomonas spp. are known to be the main determinants of the shelf-life of pasteurized milk and refrigerated raw milk. It is presumed that they mainly cause spoilage through the elaboration of proteinase and lipase enzymes. At the time of this research, under the relevant European Directive, one of the means of determining the quality of pasteurized milk was the pre-incubated count, which involves incubating the milk sample for 5 d at 6°C followed by a plate count. Examination of numerous pre-incubated counts revealed a bimodal rather than a normal distribution indicating that the types of contaminants in pasteurized milk may be as important as their initial concentration. Pseudomonads that gave particularly high (>5×106 cfu/ml) and low (<103 cfu/ml) pre-incubated counts were isolated (high and low count isolates respectively). After the organisms had been subjected to a cold shock no consistent trend between the groups of isolates was detected with respect to lag phase duration. However, the high count isolates consistently had a faster exponential growth rate. Unexpectedly, with the exception of one isolate, the low count isolates produced detectable proteinase and lipase earlier. In addition, with one exception, maximal proteinase and lipase production was observed with the low count isolates. These findings indicate that there is no causal relationship between selective growth advantage and ability to produce proteinase and lipase. It also indicates that the spoilage of pasteurized milk is a complex phenomenon and is worthy of further research.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>12916824</pmid><doi>10.1017/S0022029903006204</doi><tpages>4</tpages></addata></record> |
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| ispartof | Journal of dairy research, 2003-08, Vol.70 (3), p.293-296 |
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| subjects | Animals Biological and medical sciences Cattle - physiology cold stress Colony Count, Microbial - veterinary Contaminants Endopeptidases - metabolism Female Food Handling - methods Food industries Food Microbiology Food Preservation - methods Fundamental and applied biological sciences. Psychology Growth kinetics Kinetics Lipase - metabolism Milk Milk - microbiology Milk and cheese industries. Ice creams Pasteurization pasteurized milk plate count pollutants proteinases Pseudomonas Pseudomonas - enzymology Pseudomonas - growth & development Psychrotrophs raw milk shelf life spoilage triacylglycerol lipase |
| title | Growth kinetics and hydrolytic enzyme production of Pseudomonas spp. isolated from pasteurized milk |
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