New Insights into the Neural Differentiation Potential of Canine Adipose Tissue‐Derived Mesenchymal Stem Cells
Summary Adipose tissue‐derived stem cells (ASCs) can be obtained from different adipose tissue sources within the body. It is an abundant cell pool, easily accessible, suitable for cultivation and expansion in vitro and preparation for therapeutic approaches. Amongst these therapeutic approaches are...
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| Veröffentlicht in: | Anatomia, histologia, embryologia histologia, embryologia, 2017-06, Vol.46 (3), p.304-315 |
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| creator | Blecker, D. Elashry, M. I. Heimann, M. Wenisch, S. Arnhold, S. |
| description | Summary
Adipose tissue‐derived stem cells (ASCs) can be obtained from different adipose tissue sources within the body. It is an abundant cell pool, easily accessible, suitable for cultivation and expansion in vitro and preparation for therapeutic approaches. Amongst these therapeutic approaches are tissue engineering and nervous system disorders such as spinal cord injuries. For such treatment, ASCs have to be reliably differentiated in to the neuronal direction. Therefore, we investigated the neural differentiation potential of ASCs using protocols with neurogenic inductors such as valproic acid and forskolin, while dog brain tissue served as control. Morphological changes could already be noticed 1 h after neuronal induction. Gene expression analysis revealed that the neuronal markers nestin and βIII‐tubulin as well as MAP2 were expressed after induction of neuronal differentiation. Additionally, the expression of the neurotrophic factors NGF, BDNF and GDNF was determined. Some of the neuronal markers and neurotrophic factors were already expressed in undifferentiated cells. Our findings point out that ASCs can reliably be differentiated into the neuronal lineage; therefore, these cells are a suitable cell source for cell transplantation in disorders of the central nervous system. Follow‐up studies would show the clinical benefit of these cells after transplantation. |
| doi_str_mv | 10.1111/ahe.12270 |
| format | Article |
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Adipose tissue‐derived stem cells (ASCs) can be obtained from different adipose tissue sources within the body. It is an abundant cell pool, easily accessible, suitable for cultivation and expansion in vitro and preparation for therapeutic approaches. Amongst these therapeutic approaches are tissue engineering and nervous system disorders such as spinal cord injuries. For such treatment, ASCs have to be reliably differentiated in to the neuronal direction. Therefore, we investigated the neural differentiation potential of ASCs using protocols with neurogenic inductors such as valproic acid and forskolin, while dog brain tissue served as control. Morphological changes could already be noticed 1 h after neuronal induction. Gene expression analysis revealed that the neuronal markers nestin and βIII‐tubulin as well as MAP2 were expressed after induction of neuronal differentiation. Additionally, the expression of the neurotrophic factors NGF, BDNF and GDNF was determined. Some of the neuronal markers and neurotrophic factors were already expressed in undifferentiated cells. Our findings point out that ASCs can reliably be differentiated into the neuronal lineage; therefore, these cells are a suitable cell source for cell transplantation in disorders of the central nervous system. Follow‐up studies would show the clinical benefit of these cells after transplantation.</description><identifier>ISSN: 0340-2096</identifier><identifier>EISSN: 1439-0264</identifier><identifier>DOI: 10.1111/ahe.12270</identifier><identifier>PMID: 28401575</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Adipose tissue ; Adipose Tissue - cytology ; Animals ; Biomarkers - analysis ; Brain - cytology ; Brain injury ; Brain-derived neurotrophic factor ; Brain-Derived Neurotrophic Factor - biosynthesis ; Cell Differentiation - physiology ; Cell- and Tissue-Based Therapy - veterinary ; Central nervous system ; Dogs ; Forskolin ; Gene expression ; Glial cell line-derived neurotrophic factor ; Glial Cell Line-Derived Neurotrophic Factor - biosynthesis ; Mesenchymal stem cells ; Mesenchymal Stromal Cells - cytology ; Mesenchyme ; Microtubule-Associated Proteins - biosynthesis ; Nerve growth factor ; Nerve Growth Factor - biosynthesis ; Nervous system ; Nestin ; Nestin - biosynthesis ; Neurodegenerative Diseases - therapy ; Neurodegenerative Diseases - veterinary ; Neurons - cytology ; Neurotrophic factors ; Spinal cord injuries ; Spinal Cord Injuries - therapy ; Spinal Cord Injuries - veterinary ; Stem cells ; Tissue engineering ; Transplantation ; Tubulin ; Tubulin - biosynthesis ; Valproic acid</subject><ispartof>Anatomia, histologia, embryologia, 2017-06, Vol.46 (3), p.304-315</ispartof><rights>2017 Blackwell Verlag GmbH</rights><rights>2017 Blackwell Verlag GmbH.</rights><rights>Copyright © 2017 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3530-cbd299a3f538aac35004b5d92d428fa76b8df9ca6aaf44afa54dbfdd964604523</citedby><cites>FETCH-LOGICAL-c3530-cbd299a3f538aac35004b5d92d428fa76b8df9ca6aaf44afa54dbfdd964604523</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fahe.12270$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fahe.12270$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28401575$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Blecker, D.</creatorcontrib><creatorcontrib>Elashry, M. I.</creatorcontrib><creatorcontrib>Heimann, M.</creatorcontrib><creatorcontrib>Wenisch, S.</creatorcontrib><creatorcontrib>Arnhold, S.</creatorcontrib><title>New Insights into the Neural Differentiation Potential of Canine Adipose Tissue‐Derived Mesenchymal Stem Cells</title><title>Anatomia, histologia, embryologia</title><addtitle>Anat Histol Embryol</addtitle><description>Summary
Adipose tissue‐derived stem cells (ASCs) can be obtained from different adipose tissue sources within the body. It is an abundant cell pool, easily accessible, suitable for cultivation and expansion in vitro and preparation for therapeutic approaches. Amongst these therapeutic approaches are tissue engineering and nervous system disorders such as spinal cord injuries. For such treatment, ASCs have to be reliably differentiated in to the neuronal direction. Therefore, we investigated the neural differentiation potential of ASCs using protocols with neurogenic inductors such as valproic acid and forskolin, while dog brain tissue served as control. Morphological changes could already be noticed 1 h after neuronal induction. Gene expression analysis revealed that the neuronal markers nestin and βIII‐tubulin as well as MAP2 were expressed after induction of neuronal differentiation. Additionally, the expression of the neurotrophic factors NGF, BDNF and GDNF was determined. Some of the neuronal markers and neurotrophic factors were already expressed in undifferentiated cells. Our findings point out that ASCs can reliably be differentiated into the neuronal lineage; therefore, these cells are a suitable cell source for cell transplantation in disorders of the central nervous system. Follow‐up studies would show the clinical benefit of these cells after transplantation.</description><subject>Adipose tissue</subject><subject>Adipose Tissue - cytology</subject><subject>Animals</subject><subject>Biomarkers - analysis</subject><subject>Brain - cytology</subject><subject>Brain injury</subject><subject>Brain-derived neurotrophic factor</subject><subject>Brain-Derived Neurotrophic Factor - biosynthesis</subject><subject>Cell Differentiation - physiology</subject><subject>Cell- and Tissue-Based Therapy - veterinary</subject><subject>Central nervous system</subject><subject>Dogs</subject><subject>Forskolin</subject><subject>Gene expression</subject><subject>Glial cell line-derived neurotrophic factor</subject><subject>Glial Cell Line-Derived Neurotrophic Factor - biosynthesis</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchyme</subject><subject>Microtubule-Associated Proteins - biosynthesis</subject><subject>Nerve growth factor</subject><subject>Nerve Growth Factor - biosynthesis</subject><subject>Nervous system</subject><subject>Nestin</subject><subject>Nestin - biosynthesis</subject><subject>Neurodegenerative Diseases - therapy</subject><subject>Neurodegenerative Diseases - veterinary</subject><subject>Neurons - cytology</subject><subject>Neurotrophic factors</subject><subject>Spinal cord injuries</subject><subject>Spinal Cord Injuries - therapy</subject><subject>Spinal Cord Injuries - veterinary</subject><subject>Stem cells</subject><subject>Tissue engineering</subject><subject>Transplantation</subject><subject>Tubulin</subject><subject>Tubulin - biosynthesis</subject><subject>Valproic acid</subject><issn>0340-2096</issn><issn>1439-0264</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10c9OFTEUBvDGaOQCLnwB08SNLAb6d2a6vLmgkCCQgOumMz31lsy013ZGcnc-gs_ok1i46MLEbpo2v3w5OR9Cbyk5puWcmDUcU8Ya8gItqOCqIqwWL9GCcEEqRlS9h_Zzviekplw1r9EeawWhspELtLmCB3wRsv-6njL2YYp4WgO-gjmZAZ965yBBmLyZfAz4Jk5PjwFHh1cm-AB4af0mZsB3PucZfv34eQrJfweLP0OG0K-3Y-G3E4x4BcOQD9ErZ4YMb57vA_Tl49nd6ry6vP50sVpeVj2XnFR9Z5lShjvJW2PKHyGik1YxK1jrTFN3rXWqN7UxTgjjjBS2c9aqWtRESMYP0Idd7ibFbzPkSY8-92UCEyDOWdO2bYgUjeKFvv-H3sc5hTKdpooRwankj-pop_oUc07g9Cb50aStpkQ_1qBLDfqphmLfPSfO3Qj2r_yz9wJOduDBD7D9f5Jenp_tIn8DfGaSkw</recordid><startdate>201706</startdate><enddate>201706</enddate><creator>Blecker, D.</creator><creator>Elashry, M. I.</creator><creator>Heimann, M.</creator><creator>Wenisch, S.</creator><creator>Arnhold, S.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>201706</creationdate><title>New Insights into the Neural Differentiation Potential of Canine Adipose Tissue‐Derived Mesenchymal Stem Cells</title><author>Blecker, D. ; Elashry, M. I. ; Heimann, M. ; Wenisch, S. ; Arnhold, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3530-cbd299a3f538aac35004b5d92d428fa76b8df9ca6aaf44afa54dbfdd964604523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adipose tissue</topic><topic>Adipose Tissue - cytology</topic><topic>Animals</topic><topic>Biomarkers - analysis</topic><topic>Brain - cytology</topic><topic>Brain injury</topic><topic>Brain-derived neurotrophic factor</topic><topic>Brain-Derived Neurotrophic Factor - biosynthesis</topic><topic>Cell Differentiation - physiology</topic><topic>Cell- and Tissue-Based Therapy - veterinary</topic><topic>Central nervous system</topic><topic>Dogs</topic><topic>Forskolin</topic><topic>Gene expression</topic><topic>Glial cell line-derived neurotrophic factor</topic><topic>Glial Cell Line-Derived Neurotrophic Factor - biosynthesis</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchyme</topic><topic>Microtubule-Associated Proteins - biosynthesis</topic><topic>Nerve growth factor</topic><topic>Nerve Growth Factor - biosynthesis</topic><topic>Nervous system</topic><topic>Nestin</topic><topic>Nestin - biosynthesis</topic><topic>Neurodegenerative Diseases - therapy</topic><topic>Neurodegenerative Diseases - veterinary</topic><topic>Neurons - cytology</topic><topic>Neurotrophic factors</topic><topic>Spinal cord injuries</topic><topic>Spinal Cord Injuries - therapy</topic><topic>Spinal Cord Injuries - veterinary</topic><topic>Stem cells</topic><topic>Tissue engineering</topic><topic>Transplantation</topic><topic>Tubulin</topic><topic>Tubulin - biosynthesis</topic><topic>Valproic acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Blecker, D.</creatorcontrib><creatorcontrib>Elashry, M. I.</creatorcontrib><creatorcontrib>Heimann, M.</creatorcontrib><creatorcontrib>Wenisch, S.</creatorcontrib><creatorcontrib>Arnhold, S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Anatomia, histologia, embryologia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Blecker, D.</au><au>Elashry, M. I.</au><au>Heimann, M.</au><au>Wenisch, S.</au><au>Arnhold, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>New Insights into the Neural Differentiation Potential of Canine Adipose Tissue‐Derived Mesenchymal Stem Cells</atitle><jtitle>Anatomia, histologia, embryologia</jtitle><addtitle>Anat Histol Embryol</addtitle><date>2017-06</date><risdate>2017</risdate><volume>46</volume><issue>3</issue><spage>304</spage><epage>315</epage><pages>304-315</pages><issn>0340-2096</issn><eissn>1439-0264</eissn><abstract>Summary
Adipose tissue‐derived stem cells (ASCs) can be obtained from different adipose tissue sources within the body. It is an abundant cell pool, easily accessible, suitable for cultivation and expansion in vitro and preparation for therapeutic approaches. Amongst these therapeutic approaches are tissue engineering and nervous system disorders such as spinal cord injuries. For such treatment, ASCs have to be reliably differentiated in to the neuronal direction. Therefore, we investigated the neural differentiation potential of ASCs using protocols with neurogenic inductors such as valproic acid and forskolin, while dog brain tissue served as control. Morphological changes could already be noticed 1 h after neuronal induction. Gene expression analysis revealed that the neuronal markers nestin and βIII‐tubulin as well as MAP2 were expressed after induction of neuronal differentiation. Additionally, the expression of the neurotrophic factors NGF, BDNF and GDNF was determined. Some of the neuronal markers and neurotrophic factors were already expressed in undifferentiated cells. Our findings point out that ASCs can reliably be differentiated into the neuronal lineage; therefore, these cells are a suitable cell source for cell transplantation in disorders of the central nervous system. Follow‐up studies would show the clinical benefit of these cells after transplantation.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>28401575</pmid><doi>10.1111/ahe.12270</doi><tpages>12</tpages></addata></record> |
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| subjects | Adipose tissue Adipose Tissue - cytology Animals Biomarkers - analysis Brain - cytology Brain injury Brain-derived neurotrophic factor Brain-Derived Neurotrophic Factor - biosynthesis Cell Differentiation - physiology Cell- and Tissue-Based Therapy - veterinary Central nervous system Dogs Forskolin Gene expression Glial cell line-derived neurotrophic factor Glial Cell Line-Derived Neurotrophic Factor - biosynthesis Mesenchymal stem cells Mesenchymal Stromal Cells - cytology Mesenchyme Microtubule-Associated Proteins - biosynthesis Nerve growth factor Nerve Growth Factor - biosynthesis Nervous system Nestin Nestin - biosynthesis Neurodegenerative Diseases - therapy Neurodegenerative Diseases - veterinary Neurons - cytology Neurotrophic factors Spinal cord injuries Spinal Cord Injuries - therapy Spinal Cord Injuries - veterinary Stem cells Tissue engineering Transplantation Tubulin Tubulin - biosynthesis Valproic acid |
| title | New Insights into the Neural Differentiation Potential of Canine Adipose Tissue‐Derived Mesenchymal Stem Cells |
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