Evaluation of a 24-hour fluorogenic assay for the enumeration of Escherichia coli from foods

A 24 h assay for the detection and enumeration of Escherichia coli in foods was evaluated. The procedure used 4-methylumbelliferyl-beta-D-glucuronide (MUG) incorporated into trypticase soy agar (TSA) and violet red bile agar (VRBA). The procedure was evaluated using 106 different stock cultures, and...

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Veröffentlicht in:Journal of food protection 1988-10, Vol.51 (10), p.766-769
Hauptverfasser: Weiss, L.H, Humber, J
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Humber, J
description A 24 h assay for the detection and enumeration of Escherichia coli in foods was evaluated. The procedure used 4-methylumbelliferyl-beta-D-glucuronide (MUG) incorporated into trypticase soy agar (TSA) and violet red bile agar (VRBA). The procedure was evaluated using 106 different stock cultures, and 165 isolates from a variety of naturally contaminated foods. Samples were first surface plated on TSA-MUG, and allowed to incubate at room temperature for 1-2 h. Plates were then overlaid with VRBA-MUG and incubated at 35 degrees C for 24 h. The TSA/VRBA-MUG assay was able to detect 94.1% of the E. coli cultures tested, and 95.8% of E. coli strains isolated form foods, giving false-negative rates of 5.9% and 4.2% respectively. False positives were found with 2 of 31 Salmonella spp. isolates and 4 of 4 Shigella spp. isolates from the culture collection. There were no false positives from food isolates. The TSA/VRBA-MUG assay was able to detect E. coli in six food samples that were not detected by our routine procedure. The visible fluorescent halo around each E. coli colony gave immediate confirmation, without the need for further tests. The TSA/VRBA-MUG assay provided an accurate, simple inexpensive method for detection of E. coli, from a wide variety of food products
doi_str_mv 10.4315/0362-028X-51.10.766
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subjects Biological and medical sciences
ESCHERICHIA
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
HIDROLASAS
HIGIENE DE LOS ALIMENTOS
HYDROLASE
HYGIENE DES ALIMENTS
TECHNIQUE DE CULTURE
TECNICAS DE CULTIVO
title Evaluation of a 24-hour fluorogenic assay for the enumeration of Escherichia coli from foods
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