Bioremediation of methylene blue dye using Bacillus subtilis MTCC 441

Methylene blue (MB) commonly found in the textile industry effluent has been chosen as a model dye to investigate bioremediation using Bacillus subtilis MTCC 441. Both free cells and calcium alginate immobilized cells have been used to remove MB from the effluent. The operating variables of initial...

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Veröffentlicht in:Water science and technology 2017-04, Vol.75 (7-8), p.1572-1583
Hauptverfasser: Upendar, Ganta, Dutta, Susmita, Bhattacharya, Pinaki, Dutta, Abhishek
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container_issue 7-8
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container_title Water science and technology
container_volume 75
creator Upendar, Ganta
Dutta, Susmita
Bhattacharya, Pinaki
Dutta, Abhishek
description Methylene blue (MB) commonly found in the textile industry effluent has been chosen as a model dye to investigate bioremediation using Bacillus subtilis MTCC 441. Both free cells and calcium alginate immobilized cells have been used to remove MB from the effluent. The operating variables of initial concentration of dye (20-60 mg/L), inoculum size (4-8%) and temperature (25-35 °C) have been varied judiciously during the kinetic study in a batch contactor. A maximum removal of 91.68% is obtained when 20 mg/L MB solution was inoculated with 8% inoculum and cultured for 6 h at 30 °C. Continuous removal of MB has been studied in a fixed bed contactor using immobilized cells as packing materials. Influent concentration (10-30 mg/L) was varied and breakthrough parameters have been determined. With increase in influent concentration from 10 mg/L to 30 mg/L, percentage removal of dye decreases from 72.44% to 49.62%.
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source MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
subjects Activated carbon
Adsorption
Algae
Aqueous solutions
Bacillus subtilis - metabolism
Bacteria
Bioaccumulation
Biodegradation
Biodegradation, Environmental
Biomass
Bioremediation
Calcium
Calcium alginate
Chemical engineering
Coloring Agents - metabolism
Cyanobacteria
Dyes
Growth rate
Hydrogen-Ion Concentration
Inoculation
Inoculum
Kinetics
Metabolism
Methylene blue
Methylene Blue - metabolism
Microorganisms
Pollutants
Temperature
Textile Industry
Textile industry wastes
Water Pollutants, Chemical - metabolism
Yeast
title Bioremediation of methylene blue dye using Bacillus subtilis MTCC 441
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