Bioremediation of methylene blue dye using Bacillus subtilis MTCC 441
Methylene blue (MB) commonly found in the textile industry effluent has been chosen as a model dye to investigate bioremediation using Bacillus subtilis MTCC 441. Both free cells and calcium alginate immobilized cells have been used to remove MB from the effluent. The operating variables of initial...
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Veröffentlicht in: | Water science and technology 2017-04, Vol.75 (7-8), p.1572-1583 |
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description | Methylene blue (MB) commonly found in the textile industry effluent has been chosen as a model dye to investigate bioremediation using Bacillus subtilis MTCC 441. Both free cells and calcium alginate immobilized cells have been used to remove MB from the effluent. The operating variables of initial concentration of dye (20-60 mg/L), inoculum size (4-8%) and temperature (25-35 °C) have been varied judiciously during the kinetic study in a batch contactor. A maximum removal of 91.68% is obtained when 20 mg/L MB solution was inoculated with 8% inoculum and cultured for 6 h at 30 °C. Continuous removal of MB has been studied in a fixed bed contactor using immobilized cells as packing materials. Influent concentration (10-30 mg/L) was varied and breakthrough parameters have been determined. With increase in influent concentration from 10 mg/L to 30 mg/L, percentage removal of dye decreases from 72.44% to 49.62%. |
doi_str_mv | 10.2166/wst.2017.031 |
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Both free cells and calcium alginate immobilized cells have been used to remove MB from the effluent. The operating variables of initial concentration of dye (20-60 mg/L), inoculum size (4-8%) and temperature (25-35 °C) have been varied judiciously during the kinetic study in a batch contactor. A maximum removal of 91.68% is obtained when 20 mg/L MB solution was inoculated with 8% inoculum and cultured for 6 h at 30 °C. Continuous removal of MB has been studied in a fixed bed contactor using immobilized cells as packing materials. Influent concentration (10-30 mg/L) was varied and breakthrough parameters have been determined. 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Both free cells and calcium alginate immobilized cells have been used to remove MB from the effluent. The operating variables of initial concentration of dye (20-60 mg/L), inoculum size (4-8%) and temperature (25-35 °C) have been varied judiciously during the kinetic study in a batch contactor. A maximum removal of 91.68% is obtained when 20 mg/L MB solution was inoculated with 8% inoculum and cultured for 6 h at 30 °C. Continuous removal of MB has been studied in a fixed bed contactor using immobilized cells as packing materials. Influent concentration (10-30 mg/L) was varied and breakthrough parameters have been determined. With increase in influent concentration from 10 mg/L to 30 mg/L, percentage removal of dye decreases from 72.44% to 49.62%.</description><subject>Activated carbon</subject><subject>Adsorption</subject><subject>Algae</subject><subject>Aqueous solutions</subject><subject>Bacillus subtilis - metabolism</subject><subject>Bacteria</subject><subject>Bioaccumulation</subject><subject>Biodegradation</subject><subject>Biodegradation, Environmental</subject><subject>Biomass</subject><subject>Bioremediation</subject><subject>Calcium</subject><subject>Calcium alginate</subject><subject>Chemical engineering</subject><subject>Coloring Agents - metabolism</subject><subject>Cyanobacteria</subject><subject>Dyes</subject><subject>Growth rate</subject><subject>Hydrogen-Ion Concentration</subject><subject>Inoculation</subject><subject>Inoculum</subject><subject>Kinetics</subject><subject>Metabolism</subject><subject>Methylene blue</subject><subject>Methylene Blue - metabolism</subject><subject>Microorganisms</subject><subject>Pollutants</subject><subject>Temperature</subject><subject>Textile Industry</subject><subject>Textile industry wastes</subject><subject>Water Pollutants, Chemical - 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Both free cells and calcium alginate immobilized cells have been used to remove MB from the effluent. The operating variables of initial concentration of dye (20-60 mg/L), inoculum size (4-8%) and temperature (25-35 °C) have been varied judiciously during the kinetic study in a batch contactor. A maximum removal of 91.68% is obtained when 20 mg/L MB solution was inoculated with 8% inoculum and cultured for 6 h at 30 °C. Continuous removal of MB has been studied in a fixed bed contactor using immobilized cells as packing materials. Influent concentration (10-30 mg/L) was varied and breakthrough parameters have been determined. With increase in influent concentration from 10 mg/L to 30 mg/L, percentage removal of dye decreases from 72.44% to 49.62%.</abstract><cop>England</cop><pub>IWA Publishing</pub><pmid>28402298</pmid><doi>10.2166/wst.2017.031</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Activated carbon Adsorption Algae Aqueous solutions Bacillus subtilis - metabolism Bacteria Bioaccumulation Biodegradation Biodegradation, Environmental Biomass Bioremediation Calcium Calcium alginate Chemical engineering Coloring Agents - metabolism Cyanobacteria Dyes Growth rate Hydrogen-Ion Concentration Inoculation Inoculum Kinetics Metabolism Methylene blue Methylene Blue - metabolism Microorganisms Pollutants Temperature Textile Industry Textile industry wastes Water Pollutants, Chemical - metabolism Yeast |
title | Bioremediation of methylene blue dye using Bacillus subtilis MTCC 441 |
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