Rapid in vitro screening for the location‐dependent effects of unnatural amino acids on protein expression and activity

ABSTRACT The incorporation of unnatural amino acids (uAA) can introduce novel functional groups into proteins site‐specifically, with important applications in basic sciences and protein engineering. However, uAA incorporation can impact protein expression and functional activity depending on its lo...

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Veröffentlicht in:	Biotechnology and bioengineering 2017-10, Vol.114 (10), p.2412-2417
Hauptverfasser:	Schinn, Song‐Min, Bradley, William, Groesbeck, Ashtyn, Wu, Jeffrey C., Broadbent, Andrew, Bundy, Bradley C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Amino Acids - genetics Amino Acids - metabolism cell free protein synthesis Cloning Functional groups Gene Expression Profiling - methods High-Throughput Screening Assays - methods In vitro methods and tests in vitro screening linear DNA Lysozyme Mutants nonnatural amino acid Optimization Protein Biosynthesis - physiology Protein engineering Protein Engineering - methods Protein expression Protein purification Proteins Purification rapid screening Screening Subcellular Fractions - metabolism unnatural amino acid
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