The pore size of the autotransporter domain is critical for the active translocation of the passenger domain

The autotransporter mode of surface presentation in Gram-negative bacteria requires a hypothetical C-terminal β-barrel which makes up an aqueous channel in the outer membrane. PalA is a Pseudomonas sp. autotransporter lipolytic protein. PalA is a 66 kDa protein that is composed of two parts, the N-t...

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