Quantitative and Single-step Enzyme Immunosensing Based on an Electrochemical Detection Coupled with Lateral-flow System
A single-step electrochemical immunochromatography has been developed: the device was based on two pieces of nitrocellulose membrane, a sample pad with anti-mouse IgG antibody labeled with glucose oxidase (GOx-labeled antibody), a conjugate pad with glucose, and a Pt working electrode. Either antibo...
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| Veröffentlicht in: | Analytical Sciences 2017/04/10, Vol.33(4), pp.531-536 |
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| creator | TOMINAGA, Kohei ARIMOTO, Satoshi SHIMONO, Ken YOSHIOKA, Toshihiko MIZUTANI, Fumio YASUKAWA, Tomoyuki |
| description | A single-step electrochemical immunochromatography has been developed: the device was based on two pieces of nitrocellulose membrane, a sample pad with anti-mouse IgG antibody labeled with glucose oxidase (GOx-labeled antibody), a conjugate pad with glucose, and a Pt working electrode. Either antibody or antigen was immobilized on the membrane. The addition of a solution containing mouse IgG, a model target, allows for the dissolution of GOx-labeled antibody in the sample pad to form an immunocomplex. The produced immunocomplex was automatically separated by capturing to the antibody immobilized on the membrane with the sandwich structure or by passing through the membrane modified with an antigen for the competitive reaction. The separated GOx label arrived at the conjugate pad with glucose to undergo the enzyme reaction. Hydrogen peroxide generated by this reaction was detected at the Pt electrode prepared on the second nitrocellulose membrane downstream from the conjugate pad. The results demonstrated that the designed immunochromatography can be applied to quantitative detection with a single-step procedure, because both the GOx-labeled antibody for revealing the immunoreactions and the substrate for the enzyme reaction were prepared in the device. Moreover, the initial concentration of the GOx-labeled antibody permitted control of the detectable concentration for mouse IgG. |
| doi_str_mv | 10.2116/analsci.33.531 |
| format | Article |
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Either antibody or antigen was immobilized on the membrane. The addition of a solution containing mouse IgG, a model target, allows for the dissolution of GOx-labeled antibody in the sample pad to form an immunocomplex. The produced immunocomplex was automatically separated by capturing to the antibody immobilized on the membrane with the sandwich structure or by passing through the membrane modified with an antigen for the competitive reaction. The separated GOx label arrived at the conjugate pad with glucose to undergo the enzyme reaction. Hydrogen peroxide generated by this reaction was detected at the Pt electrode prepared on the second nitrocellulose membrane downstream from the conjugate pad. The results demonstrated that the designed immunochromatography can be applied to quantitative detection with a single-step procedure, because both the GOx-labeled antibody for revealing the immunoreactions and the substrate for the enzyme reaction were prepared in the device. Moreover, the initial concentration of the GOx-labeled antibody permitted control of the detectable concentration for mouse IgG.</description><identifier>ISSN: 0910-6340</identifier><identifier>EISSN: 1348-2246</identifier><identifier>DOI: 10.2116/analsci.33.531</identifier><identifier>PMID: 28392533</identifier><language>eng</language><publisher>Singapore: The Japan Society for Analytical Chemistry</publisher><subject>Analytical Chemistry ; Animals ; Antibodies ; Antigens ; Cellulose nitrate ; Chemistry ; Collodion - chemistry ; Dissolution ; Electrochemical analysis ; Electrochemical sensor ; Electrochemistry ; Electrodes ; enzyme label ; Enzymes ; Enzymes, Immobilized - chemistry ; Enzymes, Immobilized - metabolism ; Flow system ; Glucose ; Glucose oxidase ; Glucose Oxidase - chemistry ; Glucose Oxidase - metabolism ; Hydrogen peroxide ; Hydrogen Peroxide - chemistry ; immunochromatography ; Immunochromatography - instrumentation ; Immunochromatography - methods ; Immunoglobulin G ; lateral flow ; Membranes ; Membranes, Artificial ; Oxidation-Reduction ; single-step detection</subject><ispartof>Analytical Sciences, 2017/04/10, Vol.33(4), pp.531-536</ispartof><rights>2017 by The Japan Society for Analytical Chemistry</rights><rights>The Japan Society for Analytical Chemistry 2017</rights><rights>Copyright Japan Science and Technology Agency 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c551t-51183d5709149734bbf0ec0a6b7767483a9af6ace8bf4f3c662cc2a8f4de730a3</citedby><cites>FETCH-LOGICAL-c551t-51183d5709149734bbf0ec0a6b7767483a9af6ace8bf4f3c662cc2a8f4de730a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.2116/analsci.33.531$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.2116/analsci.33.531$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,1877,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28392533$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TOMINAGA, Kohei</creatorcontrib><creatorcontrib>ARIMOTO, Satoshi</creatorcontrib><creatorcontrib>SHIMONO, Ken</creatorcontrib><creatorcontrib>YOSHIOKA, Toshihiko</creatorcontrib><creatorcontrib>MIZUTANI, Fumio</creatorcontrib><creatorcontrib>YASUKAWA, Tomoyuki</creatorcontrib><title>Quantitative and Single-step Enzyme Immunosensing Based on an Electrochemical Detection Coupled with Lateral-flow System</title><title>Analytical Sciences</title><addtitle>ANAL. SCI</addtitle><addtitle>Anal Sci</addtitle><description>A single-step electrochemical immunochromatography has been developed: the device was based on two pieces of nitrocellulose membrane, a sample pad with anti-mouse IgG antibody labeled with glucose oxidase (GOx-labeled antibody), a conjugate pad with glucose, and a Pt working electrode. Either antibody or antigen was immobilized on the membrane. The addition of a solution containing mouse IgG, a model target, allows for the dissolution of GOx-labeled antibody in the sample pad to form an immunocomplex. The produced immunocomplex was automatically separated by capturing to the antibody immobilized on the membrane with the sandwich structure or by passing through the membrane modified with an antigen for the competitive reaction. The separated GOx label arrived at the conjugate pad with glucose to undergo the enzyme reaction. Hydrogen peroxide generated by this reaction was detected at the Pt electrode prepared on the second nitrocellulose membrane downstream from the conjugate pad. The results demonstrated that the designed immunochromatography can be applied to quantitative detection with a single-step procedure, because both the GOx-labeled antibody for revealing the immunoreactions and the substrate for the enzyme reaction were prepared in the device. Moreover, the initial concentration of the GOx-labeled antibody permitted control of the detectable concentration for mouse IgG.</description><subject>Analytical Chemistry</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antigens</subject><subject>Cellulose nitrate</subject><subject>Chemistry</subject><subject>Collodion - chemistry</subject><subject>Dissolution</subject><subject>Electrochemical analysis</subject><subject>Electrochemical sensor</subject><subject>Electrochemistry</subject><subject>Electrodes</subject><subject>enzyme label</subject><subject>Enzymes</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Enzymes, Immobilized - metabolism</subject><subject>Flow system</subject><subject>Glucose</subject><subject>Glucose oxidase</subject><subject>Glucose Oxidase - chemistry</subject><subject>Glucose Oxidase - metabolism</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - chemistry</subject><subject>immunochromatography</subject><subject>Immunochromatography - instrumentation</subject><subject>Immunochromatography - methods</subject><subject>Immunoglobulin G</subject><subject>lateral flow</subject><subject>Membranes</subject><subject>Membranes, Artificial</subject><subject>Oxidation-Reduction</subject><subject>single-step detection</subject><issn>0910-6340</issn><issn>1348-2246</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1vEzEQxS0EomnhyhFZ4sJlU3_trnMsIYVKkRAqnFez3tnEkdcbbC8l_PW4SogqJE6W_H7veTyPkDeczQXn1TV4cNHYuZTzUvJnZMal0oUQqnpOZmzBWVFJxS7IZYw7xrjQQrwkF0LLhSilnJFfXyfwySZI9idS8B29t37jsIgJ93Tlfx8GpHfDMPkxoo9Zox8gYkdHn2m6cmhSGM0WB2vA0Y-Y8oXN4nKc9i5zDzZt6RoSBnBF78YHen_I2cMr8qLPo-Pr03lFvt-uvi0_F-svn-6WN-vClCVPRcm5ll1Z56-oRS1V2_YMDYOqreuqVlrCAvoKDOq2V700VSWMEaB71WEtGcgr8v6Yuw_jjwljagYbDToHHscpNlzrvKG6rnVG3_2D7sYpPC44U4tSS1arMlPzI2XCGGPAvtkHO0A4NJw1j500p04aKZvcSTa8PcVO7YDdGf9bQgauj0DMkt9gePLu_yJvjo5dTLDBcySEZI3Dp7g6ec6a2UJo0Ms_E6ayVw</recordid><startdate>2017</startdate><enddate>2017</enddate><creator>TOMINAGA, Kohei</creator><creator>ARIMOTO, Satoshi</creator><creator>SHIMONO, Ken</creator><creator>YOSHIOKA, Toshihiko</creator><creator>MIZUTANI, Fumio</creator><creator>YASUKAWA, Tomoyuki</creator><general>The Japan Society for Analytical Chemistry</general><general>Springer Nature Singapore</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>2017</creationdate><title>Quantitative and Single-step Enzyme Immunosensing Based on an Electrochemical Detection Coupled with Lateral-flow System</title><author>TOMINAGA, Kohei ; ARIMOTO, Satoshi ; SHIMONO, Ken ; YOSHIOKA, Toshihiko ; MIZUTANI, Fumio ; YASUKAWA, Tomoyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c551t-51183d5709149734bbf0ec0a6b7767483a9af6ace8bf4f3c662cc2a8f4de730a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Analytical Chemistry</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antigens</topic><topic>Cellulose nitrate</topic><topic>Chemistry</topic><topic>Collodion - chemistry</topic><topic>Dissolution</topic><topic>Electrochemical analysis</topic><topic>Electrochemical sensor</topic><topic>Electrochemistry</topic><topic>Electrodes</topic><topic>enzyme label</topic><topic>Enzymes</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Enzymes, Immobilized - metabolism</topic><topic>Flow system</topic><topic>Glucose</topic><topic>Glucose oxidase</topic><topic>Glucose Oxidase - chemistry</topic><topic>Glucose Oxidase - metabolism</topic><topic>Hydrogen peroxide</topic><topic>Hydrogen Peroxide - chemistry</topic><topic>immunochromatography</topic><topic>Immunochromatography - instrumentation</topic><topic>Immunochromatography - methods</topic><topic>Immunoglobulin G</topic><topic>lateral flow</topic><topic>Membranes</topic><topic>Membranes, Artificial</topic><topic>Oxidation-Reduction</topic><topic>single-step detection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TOMINAGA, Kohei</creatorcontrib><creatorcontrib>ARIMOTO, Satoshi</creatorcontrib><creatorcontrib>SHIMONO, Ken</creatorcontrib><creatorcontrib>YOSHIOKA, Toshihiko</creatorcontrib><creatorcontrib>MIZUTANI, Fumio</creatorcontrib><creatorcontrib>YASUKAWA, Tomoyuki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TOMINAGA, Kohei</au><au>ARIMOTO, Satoshi</au><au>SHIMONO, Ken</au><au>YOSHIOKA, Toshihiko</au><au>MIZUTANI, Fumio</au><au>YASUKAWA, Tomoyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative and Single-step Enzyme Immunosensing Based on an Electrochemical Detection Coupled with Lateral-flow System</atitle><jtitle>Analytical Sciences</jtitle><stitle>ANAL. 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The separated GOx label arrived at the conjugate pad with glucose to undergo the enzyme reaction. Hydrogen peroxide generated by this reaction was detected at the Pt electrode prepared on the second nitrocellulose membrane downstream from the conjugate pad. The results demonstrated that the designed immunochromatography can be applied to quantitative detection with a single-step procedure, because both the GOx-labeled antibody for revealing the immunoreactions and the substrate for the enzyme reaction were prepared in the device. Moreover, the initial concentration of the GOx-labeled antibody permitted control of the detectable concentration for mouse IgG.</abstract><cop>Singapore</cop><pub>The Japan Society for Analytical Chemistry</pub><pmid>28392533</pmid><doi>10.2116/analsci.33.531</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | J-STAGE Free; MEDLINE; Springer Nature - Complete Springer Journals; Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Analytical Chemistry Animals Antibodies Antigens Cellulose nitrate Chemistry Collodion - chemistry Dissolution Electrochemical analysis Electrochemical sensor Electrochemistry Electrodes enzyme label Enzymes Enzymes, Immobilized - chemistry Enzymes, Immobilized - metabolism Flow system Glucose Glucose oxidase Glucose Oxidase - chemistry Glucose Oxidase - metabolism Hydrogen peroxide Hydrogen Peroxide - chemistry immunochromatography Immunochromatography - instrumentation Immunochromatography - methods Immunoglobulin G lateral flow Membranes Membranes, Artificial Oxidation-Reduction single-step detection |
| title | Quantitative and Single-step Enzyme Immunosensing Based on an Electrochemical Detection Coupled with Lateral-flow System |
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