Optimization of Glycyrrhiza polysaccharide liposome by response surface methodology and its immune activities
Glycyrrhiza polysaccharide liposome (GPSL) was prepared by reverse-phase evaporation method and optimized with response surface methodology. As a result, the optimum preparation conditions were as follows: the ratio of soybean phosphatide to Glycyrrhiza polysaccharide (GPS) of 24:1, temperature of d...
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| Veröffentlicht in: | International journal of biological macromolecules 2017-09, Vol.102, p.68-75 |
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| container_title | International journal of biological macromolecules |
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| creator | Wu, Yi Yi, Lei Li, Entao Li, Youying Lu, Yan Wang, Peijuan Zhou, Honglei Liu, Jiaguo Hu, Yuanliang Wang, Deyun |
| description | Glycyrrhiza polysaccharide liposome (GPSL) was prepared by reverse-phase evaporation method and optimized with response surface methodology. As a result, the optimum preparation conditions were as follows: the ratio of soybean phosphatide to Glycyrrhiza polysaccharide (GPS) of 24:1, temperature of drug incubation of 46°C, the ultrasound time of 16min. Confirmation experiments and transmission electron micrograph (SEM) exhibited the entrapment efficiency of liposome as 78.33±0.25%, with spherical shape and uniform sizes. Furthermore, proliferation assay, allogeneic mixed lymphocyte reaction and supernatant cytokines of chBM-DCs were performed by MTT and ELISA methods to investigate the immune-modulating effects of GPSL and GPS. The results showed that GPSL could significantly promote the proliferation of immature chBM-DCs, enhance the ability of mature chBM-DCs to induce T-cell proliferation and modulate the mature chBM-DCs to secret cytokines such as IL-2, IFN-γ and IL-10. These evidences indicated that the immune-modulating activity of GPS was significantly improved after encapsulated with liposome. |
| doi_str_mv | 10.1016/j.ijbiomac.2017.04.006 |
| format | Article |
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As a result, the optimum preparation conditions were as follows: the ratio of soybean phosphatide to Glycyrrhiza polysaccharide (GPS) of 24:1, temperature of drug incubation of 46°C, the ultrasound time of 16min. Confirmation experiments and transmission electron micrograph (SEM) exhibited the entrapment efficiency of liposome as 78.33±0.25%, with spherical shape and uniform sizes. Furthermore, proliferation assay, allogeneic mixed lymphocyte reaction and supernatant cytokines of chBM-DCs were performed by MTT and ELISA methods to investigate the immune-modulating effects of GPSL and GPS. The results showed that GPSL could significantly promote the proliferation of immature chBM-DCs, enhance the ability of mature chBM-DCs to induce T-cell proliferation and modulate the mature chBM-DCs to secret cytokines such as IL-2, IFN-γ and IL-10. These evidences indicated that the immune-modulating activity of GPS was significantly improved after encapsulated with liposome.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2017.04.006</identifier><identifier>PMID: 28385523</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Cell Proliferation - drug effects ; Chickens ; Cytokines - metabolism ; Gene Expression Regulation - drug effects ; Glycyrrhiza - chemistry ; Glycyrrhiza polysaccharide liposome ; Immune activities ; Immunologic Factors - administration & dosage ; Immunologic Factors - pharmacology ; Liposomes ; Male ; Models, Theoretical ; Polysaccharides - administration & dosage ; Polysaccharides - pharmacology ; Preparation and optimization ; T-Lymphocytes - cytology ; T-Lymphocytes - drug effects ; T-Lymphocytes - immunology ; Temperature ; Ultrasonic Waves</subject><ispartof>International journal of biological macromolecules, 2017-09, Vol.102, p.68-75</ispartof><rights>2017 Elsevier B.V.</rights><rights>Copyright © 2017 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-26de19354a3641baa972291695e8d9ab8f14b73513dd5c368f7d03fa90f47c873</citedby><cites>FETCH-LOGICAL-c368t-26de19354a3641baa972291695e8d9ab8f14b73513dd5c368f7d03fa90f47c873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0141813016319596$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28385523$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Yi</creatorcontrib><creatorcontrib>Yi, Lei</creatorcontrib><creatorcontrib>Li, Entao</creatorcontrib><creatorcontrib>Li, Youying</creatorcontrib><creatorcontrib>Lu, Yan</creatorcontrib><creatorcontrib>Wang, Peijuan</creatorcontrib><creatorcontrib>Zhou, Honglei</creatorcontrib><creatorcontrib>Liu, Jiaguo</creatorcontrib><creatorcontrib>Hu, Yuanliang</creatorcontrib><creatorcontrib>Wang, Deyun</creatorcontrib><title>Optimization of Glycyrrhiza polysaccharide liposome by response surface methodology and its immune activities</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>Glycyrrhiza polysaccharide liposome (GPSL) was prepared by reverse-phase evaporation method and optimized with response surface methodology. As a result, the optimum preparation conditions were as follows: the ratio of soybean phosphatide to Glycyrrhiza polysaccharide (GPS) of 24:1, temperature of drug incubation of 46°C, the ultrasound time of 16min. Confirmation experiments and transmission electron micrograph (SEM) exhibited the entrapment efficiency of liposome as 78.33±0.25%, with spherical shape and uniform sizes. Furthermore, proliferation assay, allogeneic mixed lymphocyte reaction and supernatant cytokines of chBM-DCs were performed by MTT and ELISA methods to investigate the immune-modulating effects of GPSL and GPS. The results showed that GPSL could significantly promote the proliferation of immature chBM-DCs, enhance the ability of mature chBM-DCs to induce T-cell proliferation and modulate the mature chBM-DCs to secret cytokines such as IL-2, IFN-γ and IL-10. These evidences indicated that the immune-modulating activity of GPS was significantly improved after encapsulated with liposome.</description><subject>Animals</subject><subject>Cell Proliferation - drug effects</subject><subject>Chickens</subject><subject>Cytokines - metabolism</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Glycyrrhiza - chemistry</subject><subject>Glycyrrhiza polysaccharide liposome</subject><subject>Immune activities</subject><subject>Immunologic Factors - administration & dosage</subject><subject>Immunologic Factors - pharmacology</subject><subject>Liposomes</subject><subject>Male</subject><subject>Models, Theoretical</subject><subject>Polysaccharides - administration & dosage</subject><subject>Polysaccharides - pharmacology</subject><subject>Preparation and optimization</subject><subject>T-Lymphocytes - cytology</subject><subject>T-Lymphocytes - drug effects</subject><subject>T-Lymphocytes - immunology</subject><subject>Temperature</subject><subject>Ultrasonic Waves</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVpaDZp_0LQsRc7kmXZ8q0lNB8QyCU5C1kad2exLFeSA-6vj5dNeu1p4OV5Z5iHkCvOSs54c30o8dBj8MaWFeNtyeqSseYT2XHVdgVjTHwmO8ZrXigu2Dm5SOmwpY3k6gs5r5RQUlZiR_zTnNHjX5MxTDQM9G5c7RrjfovoHMY1GWv3JqIDOuIcUvBA-5VGSHOYEtC0xMFYoB7yPrgwht8rNZOjmBNF75cJqLEZXzEjpK_kbDBjgm_v85K83P56vrkvHp_uHm5-PhZWNCoXVeOAd0LWRjQ1743p2qrqeNNJUK4zvRp43bdCcuGcPFaG1jExmI4NdWtVKy7J99PeOYY_C6SsPSYL42gmCEvSXCnZ1VJVckObE2pjSCnCoOeI3sRVc6aPqvVBf6jWR9Wa1XoTuRWv3m8svQf3r_bhdgN-nADYPn1FiDpZhMmCwwg2axfwfzfeAEw6lWw</recordid><startdate>201709</startdate><enddate>201709</enddate><creator>Wu, Yi</creator><creator>Yi, Lei</creator><creator>Li, Entao</creator><creator>Li, Youying</creator><creator>Lu, Yan</creator><creator>Wang, Peijuan</creator><creator>Zhou, Honglei</creator><creator>Liu, Jiaguo</creator><creator>Hu, Yuanliang</creator><creator>Wang, Deyun</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201709</creationdate><title>Optimization of Glycyrrhiza polysaccharide liposome by response surface methodology and its immune activities</title><author>Wu, Yi ; 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As a result, the optimum preparation conditions were as follows: the ratio of soybean phosphatide to Glycyrrhiza polysaccharide (GPS) of 24:1, temperature of drug incubation of 46°C, the ultrasound time of 16min. Confirmation experiments and transmission electron micrograph (SEM) exhibited the entrapment efficiency of liposome as 78.33±0.25%, with spherical shape and uniform sizes. Furthermore, proliferation assay, allogeneic mixed lymphocyte reaction and supernatant cytokines of chBM-DCs were performed by MTT and ELISA methods to investigate the immune-modulating effects of GPSL and GPS. The results showed that GPSL could significantly promote the proliferation of immature chBM-DCs, enhance the ability of mature chBM-DCs to induce T-cell proliferation and modulate the mature chBM-DCs to secret cytokines such as IL-2, IFN-γ and IL-10. These evidences indicated that the immune-modulating activity of GPS was significantly improved after encapsulated with liposome.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>28385523</pmid><doi>10.1016/j.ijbiomac.2017.04.006</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Cell Proliferation - drug effects Chickens Cytokines - metabolism Gene Expression Regulation - drug effects Glycyrrhiza - chemistry Glycyrrhiza polysaccharide liposome Immune activities Immunologic Factors - administration & dosage Immunologic Factors - pharmacology Liposomes Male Models, Theoretical Polysaccharides - administration & dosage Polysaccharides - pharmacology Preparation and optimization T-Lymphocytes - cytology T-Lymphocytes - drug effects T-Lymphocytes - immunology Temperature Ultrasonic Waves |
| title | Optimization of Glycyrrhiza polysaccharide liposome by response surface methodology and its immune activities |
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