Highly sensitive detection of polycyclic aromatic hydrocarbons (PAHs) and association constants of the interaction between PAHs and antibodies using surface plasmon resonance immunosensor
Surface plasmon resonance (SPR) immunoprobes for 2-hydroxybiphenyl (HBP) and benzo(a)pyrene (BaP) are fabricated with a remarkably stable immobilization of HBP–bovine serum albumin (HBP–BSA) conjugate or BaP–BSA on a SPR active material by simple physisorption. Using competitive immunoreactions of a...
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| Veröffentlicht in: | Sensors and actuators. B, Chemical Chemical, 2003-03, Vol.89 (1), p.137-143 |
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| container_title | Sensors and actuators. B, Chemical |
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| creator | Vengatajalabathy Gobi, K. Sasaki, Makoto Shoyama, Yukihiro Miura, Norio |
| description | Surface plasmon resonance (SPR) immunoprobes for 2-hydroxybiphenyl (HBP) and benzo(a)pyrene (BaP) are fabricated with a remarkably stable immobilization of HBP–bovine serum albumin (HBP–BSA) conjugate or BaP–BSA on a SPR active material by simple physisorption. Using competitive immunoreactions of antibody between antigen and antigen–protein conjugate, we demonstrate that the SPR sensors for HBP and BaP are capable of determining the concentration of HBP and BaP sensitively in the range 0.1–1000
ppb (parts-per-billion) and 0.1–300
ppb, respectively. The antibodies, anti-BaP–BSA antibody and anti-HBP–BSA antibody, anchored to the chip by antigen–antibody binding are found to be removed on treatment with a pepsin solution; the SPR chips are found to be reusable for more than 50 times with |
| doi_str_mv | 10.1016/S0925-4005(02)00455-0 |
| format | Article |
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ppb (parts-per-billion) and 0.1–300
ppb, respectively. The antibodies, anti-BaP–BSA antibody and anti-HBP–BSA antibody, anchored to the chip by antigen–antibody binding are found to be removed on treatment with a pepsin solution; the SPR chips are found to be reusable for more than 50 times with <10% decrease in the sensor signal. Association constants of the biomolecular interaction between the antigens (BaP and HBP) and the respective monoclonal antibodies are determined by considering Langmuir adsorption isotherm model. The association constants are determined to be 3.62×10
6 and 3.68×10
7
M
−1 for the immunoreactions of anti-HBP–BSA antibody with HBP in analyte and the SPR chip-immobilized HBP–BSA, respectively; similarly, the association constants are determined to be 2.66×10
6 and 7.18×10
6
M
−1 for the immunoreactions of anti-BaP–BSA antibody with BaP in analyte and the SPR chip-immobilized BaP–BSA, respectively.</description><identifier>ISSN: 0925-4005</identifier><identifier>EISSN: 1873-3077</identifier><identifier>DOI: 10.1016/S0925-4005(02)00455-0</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>2-Hydroxybiphenyl immunosensor ; Association constant ; Benzo[a]pyrene immunosensor ; Endocrine disrupters ; Pepsin ; SPR sensor</subject><ispartof>Sensors and actuators. B, Chemical, 2003-03, Vol.89 (1), p.137-143</ispartof><rights>2002 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-997b2215d7136118dee01cad97233529dff1e49500ede7d94cba83a70b8720163</citedby><cites>FETCH-LOGICAL-c404t-997b2215d7136118dee01cad97233529dff1e49500ede7d94cba83a70b8720163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0925-4005(02)00455-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Vengatajalabathy Gobi, K.</creatorcontrib><creatorcontrib>Sasaki, Makoto</creatorcontrib><creatorcontrib>Shoyama, Yukihiro</creatorcontrib><creatorcontrib>Miura, Norio</creatorcontrib><title>Highly sensitive detection of polycyclic aromatic hydrocarbons (PAHs) and association constants of the interaction between PAHs and antibodies using surface plasmon resonance immunosensor</title><title>Sensors and actuators. B, Chemical</title><description>Surface plasmon resonance (SPR) immunoprobes for 2-hydroxybiphenyl (HBP) and benzo(a)pyrene (BaP) are fabricated with a remarkably stable immobilization of HBP–bovine serum albumin (HBP–BSA) conjugate or BaP–BSA on a SPR active material by simple physisorption. Using competitive immunoreactions of antibody between antigen and antigen–protein conjugate, we demonstrate that the SPR sensors for HBP and BaP are capable of determining the concentration of HBP and BaP sensitively in the range 0.1–1000
ppb (parts-per-billion) and 0.1–300
ppb, respectively. The antibodies, anti-BaP–BSA antibody and anti-HBP–BSA antibody, anchored to the chip by antigen–antibody binding are found to be removed on treatment with a pepsin solution; the SPR chips are found to be reusable for more than 50 times with <10% decrease in the sensor signal. Association constants of the biomolecular interaction between the antigens (BaP and HBP) and the respective monoclonal antibodies are determined by considering Langmuir adsorption isotherm model. The association constants are determined to be 3.62×10
6 and 3.68×10
7
M
−1 for the immunoreactions of anti-HBP–BSA antibody with HBP in analyte and the SPR chip-immobilized HBP–BSA, respectively; similarly, the association constants are determined to be 2.66×10
6 and 7.18×10
6
M
−1 for the immunoreactions of anti-BaP–BSA antibody with BaP in analyte and the SPR chip-immobilized BaP–BSA, respectively.</description><subject>2-Hydroxybiphenyl immunosensor</subject><subject>Association constant</subject><subject>Benzo[a]pyrene immunosensor</subject><subject>Endocrine disrupters</subject><subject>Pepsin</subject><subject>SPR sensor</subject><issn>0925-4005</issn><issn>1873-3077</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqFkcGK1TAUhoMoeB19BCErmVl0PGna23YlwzB6BwYU1HVIk9O5kTa55qQjfTZfzvRW3LpKSP7vJycfY28FXAsQ-_dfoSvrogKoL6G8AqjquoBnbCfaRhYSmuY52_2LvGSviH5ATsk97Njvg3s8jgsn9OSSe0JuMaFJLngeBn4K42IWMzrDdQyTTnlzXGwMRsc-eOKXX24OdMW1t1wTBeP0GTX5LmmfaC1JR-TOJ4x66-0x_UL0fEU30ifXB-uQ-EzOP3Ka46AN8tOoacpERApe-3zipmn2YX1tiK_Zi0GPhG_-rhfs-8e7b7eH4uHzp_vbm4fCVFClouuavixFbRsh90K0FhGE0bZrSinrsrPDILDqagC02NiuMr1upW6gb5sy_6-8YO-23lMMP2ekpCZHBsdRewwzKdG2ddt2MgfrLWhiIIo4qFN0k46LEqBWVeqsSq0eFJTqrEpB5j5sHOYpnhxGRcZhHte6mF0oG9x_Gv4AXN-gOQ</recordid><startdate>20030301</startdate><enddate>20030301</enddate><creator>Vengatajalabathy Gobi, K.</creator><creator>Sasaki, Makoto</creator><creator>Shoyama, Yukihiro</creator><creator>Miura, Norio</creator><general>Elsevier B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20030301</creationdate><title>Highly sensitive detection of polycyclic aromatic hydrocarbons (PAHs) and association constants of the interaction between PAHs and antibodies using surface plasmon resonance immunosensor</title><author>Vengatajalabathy Gobi, K. ; Sasaki, Makoto ; Shoyama, Yukihiro ; Miura, Norio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-997b2215d7136118dee01cad97233529dff1e49500ede7d94cba83a70b8720163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>2-Hydroxybiphenyl immunosensor</topic><topic>Association constant</topic><topic>Benzo[a]pyrene immunosensor</topic><topic>Endocrine disrupters</topic><topic>Pepsin</topic><topic>SPR sensor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vengatajalabathy Gobi, K.</creatorcontrib><creatorcontrib>Sasaki, Makoto</creatorcontrib><creatorcontrib>Shoyama, Yukihiro</creatorcontrib><creatorcontrib>Miura, Norio</creatorcontrib><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Sensors and actuators. B, Chemical</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vengatajalabathy Gobi, K.</au><au>Sasaki, Makoto</au><au>Shoyama, Yukihiro</au><au>Miura, Norio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Highly sensitive detection of polycyclic aromatic hydrocarbons (PAHs) and association constants of the interaction between PAHs and antibodies using surface plasmon resonance immunosensor</atitle><jtitle>Sensors and actuators. B, Chemical</jtitle><date>2003-03-01</date><risdate>2003</risdate><volume>89</volume><issue>1</issue><spage>137</spage><epage>143</epage><pages>137-143</pages><issn>0925-4005</issn><eissn>1873-3077</eissn><abstract>Surface plasmon resonance (SPR) immunoprobes for 2-hydroxybiphenyl (HBP) and benzo(a)pyrene (BaP) are fabricated with a remarkably stable immobilization of HBP–bovine serum albumin (HBP–BSA) conjugate or BaP–BSA on a SPR active material by simple physisorption. Using competitive immunoreactions of antibody between antigen and antigen–protein conjugate, we demonstrate that the SPR sensors for HBP and BaP are capable of determining the concentration of HBP and BaP sensitively in the range 0.1–1000
ppb (parts-per-billion) and 0.1–300
ppb, respectively. The antibodies, anti-BaP–BSA antibody and anti-HBP–BSA antibody, anchored to the chip by antigen–antibody binding are found to be removed on treatment with a pepsin solution; the SPR chips are found to be reusable for more than 50 times with <10% decrease in the sensor signal. Association constants of the biomolecular interaction between the antigens (BaP and HBP) and the respective monoclonal antibodies are determined by considering Langmuir adsorption isotherm model. The association constants are determined to be 3.62×10
6 and 3.68×10
7
M
−1 for the immunoreactions of anti-HBP–BSA antibody with HBP in analyte and the SPR chip-immobilized HBP–BSA, respectively; similarly, the association constants are determined to be 2.66×10
6 and 7.18×10
6
M
−1 for the immunoreactions of anti-BaP–BSA antibody with BaP in analyte and the SPR chip-immobilized BaP–BSA, respectively.</abstract><pub>Elsevier B.V</pub><doi>10.1016/S0925-4005(02)00455-0</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0925-4005 |
| ispartof | Sensors and actuators. B, Chemical, 2003-03, Vol.89 (1), p.137-143 |
| issn | 0925-4005 1873-3077 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_18858893 |
| source | Elsevier ScienceDirect Journals Complete |
| subjects | 2-Hydroxybiphenyl immunosensor Association constant Benzo[a]pyrene immunosensor Endocrine disrupters Pepsin SPR sensor |
| title | Highly sensitive detection of polycyclic aromatic hydrocarbons (PAHs) and association constants of the interaction between PAHs and antibodies using surface plasmon resonance immunosensor |
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