An Optimized DNA Analysis Workflow for the Sampling, Extraction, and Concentration of DNA obtained from Archived Latent Fingerprints
DNA profiles have been obtained from fingerprints, but there is limited knowledge regarding DNA analysis from archived latent fingerprints—touch DNA “sandwiched” between adhesive and paper. Thus, this study sought to comparatively analyze a variety of collection and analytical methods in an effort t...
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| Veröffentlicht in: | Journal of forensic sciences 2018-01, Vol.63 (1), p.47-57 |
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| container_title | Journal of forensic sciences |
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| creator | Solomon, April D. Hytinen, Madison E. McClain, Aryn M. Miller, Marilyn T. Dawson Cruz, Tracey |
| description | DNA profiles have been obtained from fingerprints, but there is limited knowledge regarding DNA analysis from archived latent fingerprints—touch DNA “sandwiched” between adhesive and paper. Thus, this study sought to comparatively analyze a variety of collection and analytical methods in an effort to seek an optimized workflow for this specific sample type. Untreated and treated archived latent fingerprints were utilized to compare different biological sampling techniques, swab diluents, DNA extraction systems, DNA concentration practices, and post‐amplification purification methods. Archived latent fingerprints disassembled and sampled via direct cutting, followed by DNA extracted using the QIAamp® DNA Investigator Kit, and concentration with Centri‐Sep™ columns increased the odds of obtaining an STR profile. Using the recommended DNA workflow, 9 of the 10 samples provided STR profiles, which included 7–100% of the expected STR alleles and two full profiles. Thus, with carefully selected procedures, archived latent fingerprints can be a viable DNA source for criminal investigations including cold/postconviction cases. |
| doi_str_mv | 10.1111/1556-4029.13504 |
| format | Article |
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Thus, this study sought to comparatively analyze a variety of collection and analytical methods in an effort to seek an optimized workflow for this specific sample type. Untreated and treated archived latent fingerprints were utilized to compare different biological sampling techniques, swab diluents, DNA extraction systems, DNA concentration practices, and post‐amplification purification methods. Archived latent fingerprints disassembled and sampled via direct cutting, followed by DNA extracted using the QIAamp® DNA Investigator Kit, and concentration with Centri‐Sep™ columns increased the odds of obtaining an STR profile. Using the recommended DNA workflow, 9 of the 10 samples provided STR profiles, which included 7–100% of the expected STR alleles and two full profiles. 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Thus, this study sought to comparatively analyze a variety of collection and analytical methods in an effort to seek an optimized workflow for this specific sample type. Untreated and treated archived latent fingerprints were utilized to compare different biological sampling techniques, swab diluents, DNA extraction systems, DNA concentration practices, and post‐amplification purification methods. Archived latent fingerprints disassembled and sampled via direct cutting, followed by DNA extracted using the QIAamp® DNA Investigator Kit, and concentration with Centri‐Sep™ columns increased the odds of obtaining an STR profile. Using the recommended DNA workflow, 9 of the 10 samples provided STR profiles, which included 7–100% of the expected STR alleles and two full profiles. Thus, with carefully selected procedures, archived latent fingerprints can be a viable DNA source for criminal investigations including cold/postconviction cases.</description><subject>adhesive tape</subject><subject>archived latent fingerprints</subject><subject>Crime</subject><subject>Deoxyribonucleic acid</subject><subject>Dermatoglyphics</subject><subject>Dismantling</subject><subject>DNA</subject><subject>DNA - isolation & purification</subject><subject>DNA Fingerprinting</subject><subject>DNA typing</subject><subject>Fingerprints</subject><subject>forensic science</subject><subject>Forensic sciences</subject><subject>Humans</subject><subject>Latent fingerprints</subject><subject>Microsatellite Repeats</subject><subject>paper substrate</subject><subject>Sampling</subject><subject>short tandem repeat typing</subject><subject>Specimen Handling - methods</subject><subject>Touch</subject><subject>touch DNA</subject><subject>Workflow</subject><issn>0022-1198</issn><issn>1556-4029</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkb1vEzEYhy1UREPpzIYsdWHotf6OPZ5Cw4eiZgDU0XJ8NnW5s4N9oYS5f3idpnRgqRfLPz1-pPf9AfAWozNczznmXDQMEXWGKUfsBZg8JQdgghAhDcZKHoLXpdwghAQW-BU4JJJKIpiYgLs2wuV6DEP46zr44bKFbTT9toQCr1L-6ft0C33KcLx28KsZ1n2IP07hxZ8xGzuGFE-hiR2cpWhdrNkugsk_iNJqNCFWq89pgG221-F3fS3MWFE4ryKX1znEsbwBL73pizt-vI_A9_nFt9mnZrH8-HnWLhpLp5g1khlikfCOE-Lk1JuVInwlqemcx1Ou6rQdYoIS3hHccWWFMp3lgiLGiBCGHoH3e-86p18bV0Y9hGJd35vo0qZoLCWTCimmKnryH3qTNrmupmiCEKUUKYkrdb6nbE6lZOd1HWgweasx0ruC9K4OvatDPxRUf7x79G5Wg-ue-H-NVIDvgdvQu-1zPv1lvtyL7wEgAZkk</recordid><startdate>201801</startdate><enddate>201801</enddate><creator>Solomon, April D.</creator><creator>Hytinen, Madison E.</creator><creator>McClain, Aryn M.</creator><creator>Miller, Marilyn T.</creator><creator>Dawson Cruz, Tracey</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K7.</scope><scope>7X8</scope></search><sort><creationdate>201801</creationdate><title>An Optimized DNA Analysis Workflow for the Sampling, Extraction, and Concentration of DNA obtained from Archived Latent Fingerprints</title><author>Solomon, April D. ; Hytinen, Madison E. ; McClain, Aryn M. ; Miller, Marilyn T. ; Dawson Cruz, Tracey</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3714-84a2c06fe522e87fab925b83adef1759022d046325d21d59c69adc563044266a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>adhesive tape</topic><topic>archived latent fingerprints</topic><topic>Crime</topic><topic>Deoxyribonucleic acid</topic><topic>Dermatoglyphics</topic><topic>Dismantling</topic><topic>DNA</topic><topic>DNA - isolation & purification</topic><topic>DNA Fingerprinting</topic><topic>DNA typing</topic><topic>Fingerprints</topic><topic>forensic science</topic><topic>Forensic sciences</topic><topic>Humans</topic><topic>Latent fingerprints</topic><topic>Microsatellite Repeats</topic><topic>paper substrate</topic><topic>Sampling</topic><topic>short tandem repeat typing</topic><topic>Specimen Handling - methods</topic><topic>Touch</topic><topic>touch DNA</topic><topic>Workflow</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Solomon, April D.</creatorcontrib><creatorcontrib>Hytinen, Madison E.</creatorcontrib><creatorcontrib>McClain, Aryn M.</creatorcontrib><creatorcontrib>Miller, Marilyn T.</creatorcontrib><creatorcontrib>Dawson Cruz, Tracey</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Criminal Justice (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of forensic sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Solomon, April D.</au><au>Hytinen, Madison E.</au><au>McClain, Aryn M.</au><au>Miller, Marilyn T.</au><au>Dawson Cruz, Tracey</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An Optimized DNA Analysis Workflow for the Sampling, Extraction, and Concentration of DNA obtained from Archived Latent Fingerprints</atitle><jtitle>Journal of forensic sciences</jtitle><addtitle>J Forensic Sci</addtitle><date>2018-01</date><risdate>2018</risdate><volume>63</volume><issue>1</issue><spage>47</spage><epage>57</epage><pages>47-57</pages><issn>0022-1198</issn><eissn>1556-4029</eissn><abstract>DNA profiles have been obtained from fingerprints, but there is limited knowledge regarding DNA analysis from archived latent fingerprints—touch DNA “sandwiched” between adhesive and paper. Thus, this study sought to comparatively analyze a variety of collection and analytical methods in an effort to seek an optimized workflow for this specific sample type. Untreated and treated archived latent fingerprints were utilized to compare different biological sampling techniques, swab diluents, DNA extraction systems, DNA concentration practices, and post‐amplification purification methods. Archived latent fingerprints disassembled and sampled via direct cutting, followed by DNA extracted using the QIAamp® DNA Investigator Kit, and concentration with Centri‐Sep™ columns increased the odds of obtaining an STR profile. Using the recommended DNA workflow, 9 of the 10 samples provided STR profiles, which included 7–100% of the expected STR alleles and two full profiles. 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| fulltext | fulltext |
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| ispartof | Journal of forensic sciences, 2018-01, Vol.63 (1), p.47-57 |
| issn | 0022-1198 1556-4029 |
| language | eng |
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| source | MEDLINE; Wiley Journals |
| subjects | adhesive tape archived latent fingerprints Crime Deoxyribonucleic acid Dermatoglyphics Dismantling DNA DNA - isolation & purification DNA Fingerprinting DNA typing Fingerprints forensic science Forensic sciences Humans Latent fingerprints Microsatellite Repeats paper substrate Sampling short tandem repeat typing Specimen Handling - methods Touch touch DNA Workflow |
| title | An Optimized DNA Analysis Workflow for the Sampling, Extraction, and Concentration of DNA obtained from Archived Latent Fingerprints |
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