Intracellular Cytokines Produced by Stimulated CD3+ Cells from Chronic Myeloid Leukemia Patients
Our work examined the production of intracellular interferon (INF)-γ, tumor necrosis factor (TNF)-α, interleukin (IL)-2, and IL-4 by in vitro stimulated CD3+ cells from 38 chronic myeloid leukemia (CML) patients. At the time of diagnosis the percentages of cells producing INF-γ, TNF-α, and IL-2 were...
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| Veröffentlicht in: | Acta haematologica 2017-01, Vol.137 (3), p.148-157 |
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| container_title | Acta haematologica |
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| creator | Ptackova, Pavlina Petrackova, Martina Hindos, Miroslav Duskova, Martina Hamsikova, Eva Klamova, Hana Pecherkova, Pavla Humlova, Zuzana Vonka, Vladimir |
| description | Our work examined the production of intracellular interferon (INF)-γ, tumor necrosis factor (TNF)-α, interleukin (IL)-2, and IL-4 by in vitro stimulated CD3+ cells from 38 chronic myeloid leukemia (CML) patients. At the time of diagnosis the percentages of cells producing INF-γ, TNF-α, and IL-2 were strongly suppressed compared to those in healthy control subjects. Hematological remission achieved through treatment with tyrosine-kinase inhibitors was associated with a highly significant increase in the ratio of cells producing all 4 cytokines. The percentages of CD3+ cells producing cytokines were dependent on age, more so in CML patients than in healthy controls, and they negatively correlated with the number of leukocytes. Patients with an optimal therapy outcome possessed higher percentages of cytokine-producing CD3+ cells at diagnosis than those with nonoptimal outcomes. This difference was statistically significant in the case of INF-γ-producing cells, and it was on the brink of significance in the case of IL-2-producing cells. |
| doi_str_mv | 10.1159/000458703 |
| format | Article |
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At the time of diagnosis the percentages of cells producing INF-γ, TNF-α, and IL-2 were strongly suppressed compared to those in healthy control subjects. Hematological remission achieved through treatment with tyrosine-kinase inhibitors was associated with a highly significant increase in the ratio of cells producing all 4 cytokines. The percentages of CD3+ cells producing cytokines were dependent on age, more so in CML patients than in healthy controls, and they negatively correlated with the number of leukocytes. Patients with an optimal therapy outcome possessed higher percentages of cytokine-producing CD3+ cells at diagnosis than those with nonoptimal outcomes. This difference was statistically significant in the case of INF-γ-producing cells, and it was on the brink of significance in the case of IL-2-producing cells.</description><identifier>ISSN: 0001-5792</identifier><identifier>EISSN: 1421-9662</identifier><identifier>DOI: 10.1159/000458703</identifier><identifier>PMID: 28376476</identifier><language>eng</language><publisher>Basel, Switzerland</publisher><subject>Adult ; Age Factors ; Aged ; Aged, 80 and over ; Case-Control Studies ; CD3 Complex - metabolism ; Cytokines - biosynthesis ; Female ; Humans ; In Vitro Techniques ; Interferon-gamma - biosynthesis ; Interleukin-2 - biosynthesis ; Interleukin-4 - biosynthesis ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - drug therapy ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - immunology ; Male ; Middle Aged ; Original Paper ; T-Lymphocytes - immunology ; Tumor Burden - immunology ; Tumor Necrosis Factor-alpha - biosynthesis</subject><ispartof>Acta haematologica, 2017-01, Vol.137 (3), p.148-157</ispartof><rights>2017 S. 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Karger AG, Basel.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c306t-d0cd3eaf16196871dbb1f9c0c5ce8fb98ab690fef5863fd9dcf0faea06dd3e223</citedby><cites>FETCH-LOGICAL-c306t-d0cd3eaf16196871dbb1f9c0c5ce8fb98ab690fef5863fd9dcf0faea06dd3e223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2429,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28376476$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ptackova, Pavlina</creatorcontrib><creatorcontrib>Petrackova, Martina</creatorcontrib><creatorcontrib>Hindos, Miroslav</creatorcontrib><creatorcontrib>Duskova, Martina</creatorcontrib><creatorcontrib>Hamsikova, Eva</creatorcontrib><creatorcontrib>Klamova, Hana</creatorcontrib><creatorcontrib>Pecherkova, Pavla</creatorcontrib><creatorcontrib>Humlova, Zuzana</creatorcontrib><creatorcontrib>Vonka, Vladimir</creatorcontrib><title>Intracellular Cytokines Produced by Stimulated CD3+ Cells from Chronic Myeloid Leukemia Patients</title><title>Acta haematologica</title><addtitle>Acta Haematol</addtitle><description>Our work examined the production of intracellular interferon (INF)-γ, tumor necrosis factor (TNF)-α, interleukin (IL)-2, and IL-4 by in vitro stimulated CD3+ cells from 38 chronic myeloid leukemia (CML) patients. At the time of diagnosis the percentages of cells producing INF-γ, TNF-α, and IL-2 were strongly suppressed compared to those in healthy control subjects. Hematological remission achieved through treatment with tyrosine-kinase inhibitors was associated with a highly significant increase in the ratio of cells producing all 4 cytokines. The percentages of CD3+ cells producing cytokines were dependent on age, more so in CML patients than in healthy controls, and they negatively correlated with the number of leukocytes. Patients with an optimal therapy outcome possessed higher percentages of cytokine-producing CD3+ cells at diagnosis than those with nonoptimal outcomes. This difference was statistically significant in the case of INF-γ-producing cells, and it was on the brink of significance in the case of IL-2-producing cells.</description><subject>Adult</subject><subject>Age Factors</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Case-Control Studies</subject><subject>CD3 Complex - metabolism</subject><subject>Cytokines - biosynthesis</subject><subject>Female</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Interleukin-2 - biosynthesis</subject><subject>Interleukin-4 - biosynthesis</subject><subject>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - drug therapy</subject><subject>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - immunology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Original Paper</subject><subject>T-Lymphocytes - immunology</subject><subject>Tumor Burden - immunology</subject><subject>Tumor Necrosis Factor-alpha - biosynthesis</subject><issn>0001-5792</issn><issn>1421-9662</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtPwzAQhC0EglI4cEfIRxAK2HHi2EcUXpWKqAScg2OvITSPYieH_HtctfS0mt1vRtpB6IySG0pTeUsISVKREbaHJjSJaSQ5j_fRJOxplGYyPkLH3v8EFWdMHqKjWLCMJxmfoM9Z2zuloa6HWjmcj323rFrweOE6M2gwuBzxW1814dwHld-za5wH3GPrugbn365rK41fRqi7yuA5DEtoKoUXqq-g7f0JOrCq9nC6nVP08fjwnj9H89enWX43jzQjvI8M0YaBspRTyUVGTVlSKzXRqQZhSylUySWxYFPBmTXSaEusAkW4Cb44ZlN0ucldue53AN8XTeXXf6kWusEXVIgk4QkjaUCvNqh2nfcObLFyVaPcWFBSrAstdoUG9mIbO5QNmB3532AAzjfAUrkvcDtg6_8DxvV6ew</recordid><startdate>20170101</startdate><enddate>20170101</enddate><creator>Ptackova, Pavlina</creator><creator>Petrackova, Martina</creator><creator>Hindos, Miroslav</creator><creator>Duskova, Martina</creator><creator>Hamsikova, Eva</creator><creator>Klamova, Hana</creator><creator>Pecherkova, Pavla</creator><creator>Humlova, Zuzana</creator><creator>Vonka, Vladimir</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20170101</creationdate><title>Intracellular Cytokines Produced by Stimulated CD3+ Cells from Chronic Myeloid Leukemia Patients</title><author>Ptackova, Pavlina ; Petrackova, Martina ; Hindos, Miroslav ; Duskova, Martina ; Hamsikova, Eva ; Klamova, Hana ; Pecherkova, Pavla ; Humlova, Zuzana ; Vonka, Vladimir</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c306t-d0cd3eaf16196871dbb1f9c0c5ce8fb98ab690fef5863fd9dcf0faea06dd3e223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>Age Factors</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Case-Control Studies</topic><topic>CD3 Complex - metabolism</topic><topic>Cytokines - biosynthesis</topic><topic>Female</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Interferon-gamma - biosynthesis</topic><topic>Interleukin-2 - biosynthesis</topic><topic>Interleukin-4 - biosynthesis</topic><topic>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - drug therapy</topic><topic>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - immunology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Original Paper</topic><topic>T-Lymphocytes - immunology</topic><topic>Tumor Burden - immunology</topic><topic>Tumor Necrosis Factor-alpha - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ptackova, Pavlina</creatorcontrib><creatorcontrib>Petrackova, Martina</creatorcontrib><creatorcontrib>Hindos, Miroslav</creatorcontrib><creatorcontrib>Duskova, Martina</creatorcontrib><creatorcontrib>Hamsikova, Eva</creatorcontrib><creatorcontrib>Klamova, Hana</creatorcontrib><creatorcontrib>Pecherkova, Pavla</creatorcontrib><creatorcontrib>Humlova, Zuzana</creatorcontrib><creatorcontrib>Vonka, Vladimir</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta haematologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ptackova, Pavlina</au><au>Petrackova, Martina</au><au>Hindos, Miroslav</au><au>Duskova, Martina</au><au>Hamsikova, Eva</au><au>Klamova, Hana</au><au>Pecherkova, Pavla</au><au>Humlova, Zuzana</au><au>Vonka, Vladimir</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intracellular Cytokines Produced by Stimulated CD3+ Cells from Chronic Myeloid Leukemia Patients</atitle><jtitle>Acta haematologica</jtitle><addtitle>Acta Haematol</addtitle><date>2017-01-01</date><risdate>2017</risdate><volume>137</volume><issue>3</issue><spage>148</spage><epage>157</epage><pages>148-157</pages><issn>0001-5792</issn><eissn>1421-9662</eissn><abstract>Our work examined the production of intracellular interferon (INF)-γ, tumor necrosis factor (TNF)-α, interleukin (IL)-2, and IL-4 by in vitro stimulated CD3+ cells from 38 chronic myeloid leukemia (CML) patients. At the time of diagnosis the percentages of cells producing INF-γ, TNF-α, and IL-2 were strongly suppressed compared to those in healthy control subjects. Hematological remission achieved through treatment with tyrosine-kinase inhibitors was associated with a highly significant increase in the ratio of cells producing all 4 cytokines. The percentages of CD3+ cells producing cytokines were dependent on age, more so in CML patients than in healthy controls, and they negatively correlated with the number of leukocytes. Patients with an optimal therapy outcome possessed higher percentages of cytokine-producing CD3+ cells at diagnosis than those with nonoptimal outcomes. This difference was statistically significant in the case of INF-γ-producing cells, and it was on the brink of significance in the case of IL-2-producing cells.</abstract><cop>Basel, Switzerland</cop><pmid>28376476</pmid><doi>10.1159/000458703</doi><tpages>10</tpages></addata></record> |
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| subjects | Adult Age Factors Aged Aged, 80 and over Case-Control Studies CD3 Complex - metabolism Cytokines - biosynthesis Female Humans In Vitro Techniques Interferon-gamma - biosynthesis Interleukin-2 - biosynthesis Interleukin-4 - biosynthesis Leukemia, Myelogenous, Chronic, BCR-ABL Positive - drug therapy Leukemia, Myelogenous, Chronic, BCR-ABL Positive - immunology Male Middle Aged Original Paper T-Lymphocytes - immunology Tumor Burden - immunology Tumor Necrosis Factor-alpha - biosynthesis |
| title | Intracellular Cytokines Produced by Stimulated CD3+ Cells from Chronic Myeloid Leukemia Patients |
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