Segmental isotopic labeling of a single‐domain globular protein without any refolding step by an asparaginyl endopeptidase
Asparaginyl endopeptidases (AEPs) catalyze head‐to‐tail backbone cyclization of naturally occurring cyclic peptides such as cyclotides, and have become an important peptide‐engineering tool for macrocyclization and peptide ligation. Here, we report efficient protein ligation in trans by mimicking ef...
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| Veröffentlicht in: | FEBS letters 2017-05, Vol.591 (9), p.1285-1294 |
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| creator | Mikula, Kornelia M. Tascón, Igor Tommila, Jenni J. Iwaï, Hideo |
| description | Asparaginyl endopeptidases (AEPs) catalyze head‐to‐tail backbone cyclization of naturally occurring cyclic peptides such as cyclotides, and have become an important peptide‐engineering tool for macrocyclization and peptide ligation. Here, we report efficient protein ligation in trans by mimicking efficient backbone cyclization by an AEP without any excess of reactants. We demonstrate a practical application of segmental isotopic labeling for NMR studies of a single‐domain globular protein without any refolding step using the recombinant AEP prepared from Escherichia coli. This simple protein ligation approach using an AEP could be applied for incorporation of various biophysical probes into proteins as well as post‐translational production of full‐length proteins. |
| doi_str_mv | 10.1002/1873-3468.12640 |
| format | Article |
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This simple protein ligation approach using an AEP could be applied for incorporation of various biophysical probes into proteins as well as post‐translational production of full‐length proteins.</description><subject>asparaginyl endopeptidase</subject><subject>Cysteine Endopeptidases - genetics</subject><subject>Cysteine Endopeptidases - metabolism</subject><subject>Escherichia coli - genetics</subject><subject>Isotope Labeling - methods</subject><subject>Magnetic Resonance Spectroscopy - methods</subject><subject>Models, Molecular</subject><subject>NMR</subject><subject>Oldenlandia - enzymology</subject><subject>Oldenlandia - genetics</subject><subject>Peptides, Cyclic - chemistry</subject><subject>Peptides, Cyclic - genetics</subject><subject>Peptides, Cyclic - metabolism</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Protein Conformation</subject><subject>protein ligation</subject><subject>Protein Refolding</subject><subject>Recombinant Proteins - chemistry</subject><subject>Recombinant Proteins - metabolism</subject><subject>segmental isotopic labeling</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkLFO3TAUhi1UBLfA3A157BKwHceJR0BAKyExALN1Ep9cXDlxiBOhSB36CDwjT4Ivl7Iy2f71nV8-HyE_ODvhjIlTXpV5lktVnXChJNshq8_kG1kxxmVWlDrfJ99j_MPSu-J6j-yLKle6KsWK_L3DdYf9BJ66GKYwuIZ6qNG7fk1DS4HGdPP4-u_Fhg5cT9c-1LOHkQ5jmDAFz256DPNEoV_oiG3wdjMbJxxovaSUQhxghLXrF0-xt2HAYXIWIh6S3RZ8xKOP84A8XF3eX_zKbm6vf1-c3WSNLDjLFJNSFqVEDhUr6iYt1yLUreC6kblqARpbal1aJpBpyYSuVZMrKywqWQiWH5Cf29705acZ42Q6Fxv0HnoMczS8qiRXpeQ6oadbtBlDjGkdM4yug3ExnJmNcrMRbDaCzbvyNHH8UT7XHdpP_r_jBKgt8Ow8Ll_1mavLc7FtfgPq_I4o</recordid><startdate>201705</startdate><enddate>201705</enddate><creator>Mikula, Kornelia M.</creator><creator>Tascón, Igor</creator><creator>Tommila, Jenni J.</creator><creator>Iwaï, Hideo</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201705</creationdate><title>Segmental isotopic labeling of a single‐domain globular protein without any refolding step by an asparaginyl endopeptidase</title><author>Mikula, Kornelia M. ; 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| fulltext | fulltext |
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| ispartof | FEBS letters, 2017-05, Vol.591 (9), p.1285-1294 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; Alma/SFX Local Collection |
| subjects | asparaginyl endopeptidase Cysteine Endopeptidases - genetics Cysteine Endopeptidases - metabolism Escherichia coli - genetics Isotope Labeling - methods Magnetic Resonance Spectroscopy - methods Models, Molecular NMR Oldenlandia - enzymology Oldenlandia - genetics Peptides, Cyclic - chemistry Peptides, Cyclic - genetics Peptides, Cyclic - metabolism Plant Proteins - genetics Plant Proteins - metabolism Protein Conformation protein ligation Protein Refolding Recombinant Proteins - chemistry Recombinant Proteins - metabolism segmental isotopic labeling |
| title | Segmental isotopic labeling of a single‐domain globular protein without any refolding step by an asparaginyl endopeptidase |
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