Differentiation behavior of iPS cells cultured on PLGA with osteoinduction medium
In the present report, we have generated osteoblast-like cells derived from mouse induced-pluripotent stem (iPS) cells on PLGA with osteoinduction medium in vitro and in vivo. The cell culture period was 2 weeks. At 2 weeks, mRNA level of type I collagen was significantly higher than at 1 week. Oste...
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| Veröffentlicht in: | Dental Materials Journal 2017/01/30, Vol.36(1), pp.103-110 |
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| container_title | Dental Materials Journal |
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| creator | TOKITA, Reiko NAKAJIMA, Kei INOUE, Kenji AL-WAHABI, Akram SER-OD, Tungalag MATSUZAKA, Kenichi INOUE, Takashi |
| description | In the present report, we have generated osteoblast-like cells derived from mouse induced-pluripotent stem (iPS) cells on PLGA with osteoinduction medium in vitro and in vivo. The cell culture period was 2 weeks. At 2 weeks, mRNA level of type I collagen was significantly higher than at 1 week. Osteocalcin mRNA level at 2 weeks was tendency to increase compared with at 1 week. And the cells cultured on PLGA were positive for immunofluorescent staining of osteocalcin and alizarin red S staining. The scaffold and osteogenic-like cells induced in vitro were implanted subcutaneously into SCID mice. In resected teratoma, hard tissues resembling bone were observed mixed with other tissues on the scaffold. The sum of these findings suggests that PLGA does not disturb the osteogenesis of iPS cells. |
| doi_str_mv | 10.4012/dmj.2016-087 |
| format | Article |
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The cell culture period was 2 weeks. At 2 weeks, mRNA level of type I collagen was significantly higher than at 1 week. Osteocalcin mRNA level at 2 weeks was tendency to increase compared with at 1 week. And the cells cultured on PLGA were positive for immunofluorescent staining of osteocalcin and alizarin red S staining. The scaffold and osteogenic-like cells induced in vitro were implanted subcutaneously into SCID mice. In resected teratoma, hard tissues resembling bone were observed mixed with other tissues on the scaffold. The sum of these findings suggests that PLGA does not disturb the osteogenesis of iPS cells.</description><identifier>ISSN: 0287-4547</identifier><identifier>EISSN: 1881-1361</identifier><identifier>DOI: 10.4012/dmj.2016-087</identifier><identifier>PMID: 28090031</identifier><language>eng</language><publisher>Japan: The Japanese Society for Dental Materials and Devices</publisher><subject>Alizarin ; Animal tissues ; Animals ; Biocompatibility ; Biodegradable material ; Biomedical materials ; Biotechnology ; Bones ; Cell culture ; Cell Differentiation ; Cells, Cultured ; Collagen (type I) ; Collagens ; Dentistry ; In vitro testing ; Induced Pluripotent Stem Cells ; iPS cells ; Mesenchymal Stromal Cells ; Mice ; Mice, SCID ; mRNA ; Osteocalcin ; Osteogenesis ; Osteoinduction ; PLGA ; Pluripotency ; Polylactide-co-glycolide ; Scaffolds ; Staining ; Surgical implants ; Teratoma</subject><ispartof>Dental Materials Journal, 2017/01/30, Vol.36(1), pp.103-110</ispartof><rights>2017 The Japanese Society for Dental Materials and Devices</rights><rights>Copyright Japan Science and Technology Agency 2017</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c572t-63180eec6d6e00bcea3ebe0342f194bf6039756331cba5ce0d754de1e8d6d6f53</citedby><cites>FETCH-LOGICAL-c572t-63180eec6d6e00bcea3ebe0342f194bf6039756331cba5ce0d754de1e8d6d6f53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28090031$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TOKITA, Reiko</creatorcontrib><creatorcontrib>NAKAJIMA, Kei</creatorcontrib><creatorcontrib>INOUE, Kenji</creatorcontrib><creatorcontrib>AL-WAHABI, Akram</creatorcontrib><creatorcontrib>SER-OD, Tungalag</creatorcontrib><creatorcontrib>MATSUZAKA, Kenichi</creatorcontrib><creatorcontrib>INOUE, Takashi</creatorcontrib><title>Differentiation behavior of iPS cells cultured on PLGA with osteoinduction medium</title><title>Dental Materials Journal</title><addtitle>Dent. Mater. J.</addtitle><description>In the present report, we have generated osteoblast-like cells derived from mouse induced-pluripotent stem (iPS) cells on PLGA with osteoinduction medium in vitro and in vivo. The cell culture period was 2 weeks. At 2 weeks, mRNA level of type I collagen was significantly higher than at 1 week. Osteocalcin mRNA level at 2 weeks was tendency to increase compared with at 1 week. And the cells cultured on PLGA were positive for immunofluorescent staining of osteocalcin and alizarin red S staining. The scaffold and osteogenic-like cells induced in vitro were implanted subcutaneously into SCID mice. In resected teratoma, hard tissues resembling bone were observed mixed with other tissues on the scaffold. The sum of these findings suggests that PLGA does not disturb the osteogenesis of iPS cells.</description><subject>Alizarin</subject><subject>Animal tissues</subject><subject>Animals</subject><subject>Biocompatibility</subject><subject>Biodegradable material</subject><subject>Biomedical materials</subject><subject>Biotechnology</subject><subject>Bones</subject><subject>Cell culture</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Collagen (type I)</subject><subject>Collagens</subject><subject>Dentistry</subject><subject>In vitro testing</subject><subject>Induced Pluripotent Stem Cells</subject><subject>iPS cells</subject><subject>Mesenchymal Stromal Cells</subject><subject>Mice</subject><subject>Mice, SCID</subject><subject>mRNA</subject><subject>Osteocalcin</subject><subject>Osteogenesis</subject><subject>Osteoinduction</subject><subject>PLGA</subject><subject>Pluripotency</subject><subject>Polylactide-co-glycolide</subject><subject>Scaffolds</subject><subject>Staining</subject><subject>Surgical implants</subject><subject>Teratoma</subject><issn>0287-4547</issn><issn>1881-1361</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc9P5CAYholxo-OPm2fTxIsH634USpmjjq67ySSr2fVMKP3qMGmLAtX438s4OgdPXuDA877A9xByROGcAy1-Nv3yvAAqcpDVFplQKWlOmaDbZAKFrHJe8mqX7IWwBOBTIeUO2S0kTAEYnZC7K9u26HGIVkfrhqzGhX62zmeuzeztv8xg14XMjF0cPTZZIm7nNxfZi42LzIWIzg7NaN6jPTZ27A_Ij1Z3AQ8_9n1y_-v6_-x3Pv9782d2Mc9NWRUxF4xKQDSiEQhQG9QMawTGi5ZOed0KYNOqFIxRU-vSIDRVyRukKJsUaUu2T07XvY_ePY0YouptWL1WD-jGoNIgOAUuGfsGKiTjUMB3WgUtC1YKkdCTL-jSjX5If1bJB-ci3S8TdbamjHcheGzVo7e99q-KgloJVEngKiBUEpjw44_SsU7z3MCfxhJwuQaWIeoH3ADaR2s6fG9jQtHV8tm6OTQL7RUO7A1nJaw3</recordid><startdate>2017</startdate><enddate>2017</enddate><creator>TOKITA, Reiko</creator><creator>NAKAJIMA, Kei</creator><creator>INOUE, Kenji</creator><creator>AL-WAHABI, Akram</creator><creator>SER-OD, Tungalag</creator><creator>MATSUZAKA, Kenichi</creator><creator>INOUE, Takashi</creator><general>The Japanese Society for Dental Materials and Devices</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>2017</creationdate><title>Differentiation behavior of iPS cells cultured on PLGA with osteoinduction medium</title><author>TOKITA, Reiko ; 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Mater. J.</addtitle><date>2017</date><risdate>2017</risdate><volume>36</volume><issue>1</issue><spage>103</spage><epage>110</epage><pages>103-110</pages><issn>0287-4547</issn><eissn>1881-1361</eissn><abstract>In the present report, we have generated osteoblast-like cells derived from mouse induced-pluripotent stem (iPS) cells on PLGA with osteoinduction medium in vitro and in vivo. The cell culture period was 2 weeks. At 2 weeks, mRNA level of type I collagen was significantly higher than at 1 week. Osteocalcin mRNA level at 2 weeks was tendency to increase compared with at 1 week. And the cells cultured on PLGA were positive for immunofluorescent staining of osteocalcin and alizarin red S staining. The scaffold and osteogenic-like cells induced in vitro were implanted subcutaneously into SCID mice. In resected teratoma, hard tissues resembling bone were observed mixed with other tissues on the scaffold. 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| subjects | Alizarin Animal tissues Animals Biocompatibility Biodegradable material Biomedical materials Biotechnology Bones Cell culture Cell Differentiation Cells, Cultured Collagen (type I) Collagens Dentistry In vitro testing Induced Pluripotent Stem Cells iPS cells Mesenchymal Stromal Cells Mice Mice, SCID mRNA Osteocalcin Osteogenesis Osteoinduction PLGA Pluripotency Polylactide-co-glycolide Scaffolds Staining Surgical implants Teratoma |
| title | Differentiation behavior of iPS cells cultured on PLGA with osteoinduction medium |
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