Proteomic Characteristics of Blood Serum in Rats with Different Behavioral Parameters after Acute Stress
We studied proteome profile of blood serum of Wistar rats with different behavioral activity immediately and in 1 and 3 days after acute stress on the model of 12-h immobilization during the nighttime. Comparative analysis of 2D-electrophoretograms revealed differences in the expression of serum pro...
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| Veröffentlicht in: | Bulletin of experimental biology and medicine 2017-03, Vol.162 (5), p.602-605 |
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| creator | Kirbaeva, N. V. Sharanova, N. E. Vasil’ev, A. V. Pertsov, S. S. |
| description | We studied proteome profile of blood serum of Wistar rats with different behavioral activity immediately and in 1 and 3 days after acute stress on the model of 12-h immobilization during the nighttime. Comparative analysis of 2D-electrophoretograms revealed differences in the expression of serum proteins in non-stressed (control) and stressed (experimental) rats. We found 22 protein spots that characterized the proteomic features of blood serum in rats with different prognostic resistance to stress. Mass-spectrometry of isolated spots identified 6 functional proteins. Persistent proteome changes in the blood of animals at different stages after acute stress were determined. The specificity of proteomic characteristics of blood serum was shown in behaviorally passive and active rats during the post-stress period. These data extend the concept on specific protein markers for the formation of a negative emotional state and adaptive-and-compensatory processes in mammals with different sensitivity to stressogenic factors. |
| doi_str_mv | 10.1007/s10517-017-3667-4 |
| format | Article |
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The specificity of proteomic characteristics of blood serum was shown in behaviorally passive and active rats during the post-stress period. 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V.</creatorcontrib><creatorcontrib>Sharanova, N. E.</creatorcontrib><creatorcontrib>Vasil’ev, A. V.</creatorcontrib><creatorcontrib>Pertsov, S. S.</creatorcontrib><title>Proteomic Characteristics of Blood Serum in Rats with Different Behavioral Parameters after Acute Stress</title><title>Bulletin of experimental biology and medicine</title><addtitle>Bull Exp Biol Med</addtitle><addtitle>Bull Exp Biol Med</addtitle><description>We studied proteome profile of blood serum of Wistar rats with different behavioral activity immediately and in 1 and 3 days after acute stress on the model of 12-h immobilization during the nighttime. Comparative analysis of 2D-electrophoretograms revealed differences in the expression of serum proteins in non-stressed (control) and stressed (experimental) rats. We found 22 protein spots that characterized the proteomic features of blood serum in rats with different prognostic resistance to stress. Mass-spectrometry of isolated spots identified 6 functional proteins. Persistent proteome changes in the blood of animals at different stages after acute stress were determined. The specificity of proteomic characteristics of blood serum was shown in behaviorally passive and active rats during the post-stress period. 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| subjects | Animals Biological products Biomedical and Life Sciences Biomedicine Blood Blood proteins Blood Proteins - metabolism Cell Biology Comparative analysis Cytochrome P-450 CYP2E1 - blood Emotions Immobilization Immunoglobulin Light Chains - blood Internal Medicine Laboratory Medicine Lamin Type A - blood Male Nighttime Pathology Peroxiredoxin VI - blood Proteome - metabolism Rats, Wistar Rho Guanine Nucleotide Exchange Factors - blood Rodents Serum proteins Stress, Psychological - blood |
| title | Proteomic Characteristics of Blood Serum in Rats with Different Behavioral Parameters after Acute Stress |
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