The COP9 signalosome is an essential regulator of development in the filamentous fungus Aspergillus nidulans
Summary The COP9 signalosome (CSN) is a conserved multiprotein complex involved in regulation of eukaryotic development. The deduced amino acid sequences of two Aspergillus nidulans genes, csnD and csnE, show high identities to the fourth and fifth CSN subunits of higher eukaryotes. The csnD transcr...
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| Veröffentlicht in: | Molecular microbiology 2003-08, Vol.49 (3), p.717-730 |
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| creator | Busch, Silke Eckert, Sabine E. Krappmann, Sven Braus, Gerhard H. |
| description | Summary
The COP9 signalosome (CSN) is a conserved multiprotein complex involved in regulation of eukaryotic development. The deduced amino acid sequences of two Aspergillus nidulans genes, csnD and csnE, show high identities to the fourth and fifth CSN subunits of higher eukaryotes. The csnD transcript is abundant during vegetative growth as well as development and the corresponding protein accumulates in the nucleus. Strains deleted for either csn gene are viable and show identical mutant phenotypes at conditions that allow development: hyphae appear partly red and contain cells of reduced size. Additionally, light dependence of propagation onset is affected. The Δcsn mutants are capable of initiating the sexual cycle and develop primordia, but maturation to sexual fruit bodies is blocked. This developmental arrest could not be overcome by overexpression of the sexual activator velvet (VEA). We conclude that the COP9 signalosome in A. nidulans is a key regulator of sexual development, and its proposed structural and functional conservation to the CSN of higher eukaryotes enables studies on this regulatory complex in a genetically amenable organism. |
| doi_str_mv | 10.1046/j.1365-2958.2003.03612.x |
| format | Article |
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The COP9 signalosome (CSN) is a conserved multiprotein complex involved in regulation of eukaryotic development. The deduced amino acid sequences of two Aspergillus nidulans genes, csnD and csnE, show high identities to the fourth and fifth CSN subunits of higher eukaryotes. The csnD transcript is abundant during vegetative growth as well as development and the corresponding protein accumulates in the nucleus. Strains deleted for either csn gene are viable and show identical mutant phenotypes at conditions that allow development: hyphae appear partly red and contain cells of reduced size. Additionally, light dependence of propagation onset is affected. The Δcsn mutants are capable of initiating the sexual cycle and develop primordia, but maturation to sexual fruit bodies is blocked. This developmental arrest could not be overcome by overexpression of the sexual activator velvet (VEA). We conclude that the COP9 signalosome in A. nidulans is a key regulator of sexual development, and its proposed structural and functional conservation to the CSN of higher eukaryotes enables studies on this regulatory complex in a genetically amenable organism.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1046/j.1365-2958.2003.03612.x</identifier><identifier>PMID: 12864854</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Amino Acid Sequence ; Aspergillus nidulans - genetics ; Aspergillus nidulans - growth & development ; Aspergillus nidulans - radiation effects ; Aspergillus nidulans - ultrastructure ; DNA, Complementary - genetics ; DNA-Binding Proteins - chemistry ; Fungal Proteins - genetics ; Fungal Proteins - physiology ; Gene Expression Regulation, Developmental - radiation effects ; Gene Expression Regulation, Fungal - radiation effects ; Genes, Fungal ; Light ; Molecular Sequence Data ; Morphogenesis ; Multienzyme Complexes - genetics ; Multienzyme Complexes - physiology ; Mutagenesis ; Peptide Hydrolases ; Phenotype ; Pigments, Biological - metabolism ; Protein Structure, Tertiary ; Recombinant Fusion Proteins - physiology ; Reproduction ; Sequence Alignment ; Sequence Homology, Amino Acid ; Species Specificity ; Transcription Factors - chemistry ; Transcription, Genetic</subject><ispartof>Molecular microbiology, 2003-08, Vol.49 (3), p.717-730</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5122-a854dc41e8e462ea1c821f68f92475721b23c89bbe0be12a7266ac67b7eb59703</citedby><cites>FETCH-LOGICAL-c5122-a854dc41e8e462ea1c821f68f92475721b23c89bbe0be12a7266ac67b7eb59703</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2958.2003.03612.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2958.2003.03612.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12864854$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Busch, Silke</creatorcontrib><creatorcontrib>Eckert, Sabine E.</creatorcontrib><creatorcontrib>Krappmann, Sven</creatorcontrib><creatorcontrib>Braus, Gerhard H.</creatorcontrib><title>The COP9 signalosome is an essential regulator of development in the filamentous fungus Aspergillus nidulans</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Summary
The COP9 signalosome (CSN) is a conserved multiprotein complex involved in regulation of eukaryotic development. The deduced amino acid sequences of two Aspergillus nidulans genes, csnD and csnE, show high identities to the fourth and fifth CSN subunits of higher eukaryotes. The csnD transcript is abundant during vegetative growth as well as development and the corresponding protein accumulates in the nucleus. Strains deleted for either csn gene are viable and show identical mutant phenotypes at conditions that allow development: hyphae appear partly red and contain cells of reduced size. Additionally, light dependence of propagation onset is affected. The Δcsn mutants are capable of initiating the sexual cycle and develop primordia, but maturation to sexual fruit bodies is blocked. This developmental arrest could not be overcome by overexpression of the sexual activator velvet (VEA). We conclude that the COP9 signalosome in A. nidulans is a key regulator of sexual development, and its proposed structural and functional conservation to the CSN of higher eukaryotes enables studies on this regulatory complex in a genetically amenable organism.</description><subject>Amino Acid Sequence</subject><subject>Aspergillus nidulans - genetics</subject><subject>Aspergillus nidulans - growth & development</subject><subject>Aspergillus nidulans - radiation effects</subject><subject>Aspergillus nidulans - ultrastructure</subject><subject>DNA, Complementary - genetics</subject><subject>DNA-Binding Proteins - chemistry</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - physiology</subject><subject>Gene Expression Regulation, Developmental - radiation effects</subject><subject>Gene Expression Regulation, Fungal - radiation effects</subject><subject>Genes, Fungal</subject><subject>Light</subject><subject>Molecular Sequence Data</subject><subject>Morphogenesis</subject><subject>Multienzyme Complexes - genetics</subject><subject>Multienzyme Complexes - physiology</subject><subject>Mutagenesis</subject><subject>Peptide Hydrolases</subject><subject>Phenotype</subject><subject>Pigments, Biological - metabolism</subject><subject>Protein Structure, Tertiary</subject><subject>Recombinant Fusion Proteins - physiology</subject><subject>Reproduction</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>Species Specificity</subject><subject>Transcription Factors - chemistry</subject><subject>Transcription, Genetic</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtPwzAQhC0EgvL4C8gnbgn2OnGcA4eq4iUVlQNI3Cwn3RRXTlLiBtp_j0MruHLatXZmPPoIoZzFnCXyehlzIdMI8lTFwJiImZAc4s0BGf0eDsmI5SmLhIK3E3Lq_ZIxLpgUx-SEg5KJSpMRcS_vSCez55x6u2iMa31bI7Wemoai99isrXG0w0XvzLrtaFvROX6ia1d1uFHb0HUIqKwzw7vtPa36ZhHG2K-wW1jnwt7YebA3_pwcVcZ5vNjPM_J6d_syeYims_vHyXgalSkHiExoNi8TjgoTCWh4qYBXUlU5JFmaAS9AlCovCmQFcjAZSGlKmRUZFmmeMXFGrna5q6796NGvdW19iS50wFBRc6UAeA5BqHbCsmu977DSq87WpttqzvRAWi_1AFQPQPVAWv-Q1ptgvdz_0Rc1zv-Me7RBcLMTfFmH238H66enx2ET35tAjgM</recordid><startdate>200308</startdate><enddate>200308</enddate><creator>Busch, Silke</creator><creator>Eckert, Sabine E.</creator><creator>Krappmann, Sven</creator><creator>Braus, Gerhard H.</creator><general>Blackwell Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>200308</creationdate><title>The COP9 signalosome is an essential regulator of development in the filamentous fungus Aspergillus nidulans</title><author>Busch, Silke ; Eckert, Sabine E. ; Krappmann, Sven ; Braus, Gerhard H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5122-a854dc41e8e462ea1c821f68f92475721b23c89bbe0be12a7266ac67b7eb59703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Amino Acid Sequence</topic><topic>Aspergillus nidulans - genetics</topic><topic>Aspergillus nidulans - growth & development</topic><topic>Aspergillus nidulans - radiation effects</topic><topic>Aspergillus nidulans - ultrastructure</topic><topic>DNA, Complementary - genetics</topic><topic>DNA-Binding Proteins - chemistry</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - physiology</topic><topic>Gene Expression Regulation, Developmental - radiation effects</topic><topic>Gene Expression Regulation, Fungal - radiation effects</topic><topic>Genes, Fungal</topic><topic>Light</topic><topic>Molecular Sequence Data</topic><topic>Morphogenesis</topic><topic>Multienzyme Complexes - genetics</topic><topic>Multienzyme Complexes - physiology</topic><topic>Mutagenesis</topic><topic>Peptide Hydrolases</topic><topic>Phenotype</topic><topic>Pigments, Biological - metabolism</topic><topic>Protein Structure, Tertiary</topic><topic>Recombinant Fusion Proteins - physiology</topic><topic>Reproduction</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>Species Specificity</topic><topic>Transcription Factors - chemistry</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Busch, Silke</creatorcontrib><creatorcontrib>Eckert, Sabine E.</creatorcontrib><creatorcontrib>Krappmann, Sven</creatorcontrib><creatorcontrib>Braus, Gerhard H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Busch, Silke</au><au>Eckert, Sabine E.</au><au>Krappmann, Sven</au><au>Braus, Gerhard H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The COP9 signalosome is an essential regulator of development in the filamentous fungus Aspergillus nidulans</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>2003-08</date><risdate>2003</risdate><volume>49</volume><issue>3</issue><spage>717</spage><epage>730</epage><pages>717-730</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
The COP9 signalosome (CSN) is a conserved multiprotein complex involved in regulation of eukaryotic development. The deduced amino acid sequences of two Aspergillus nidulans genes, csnD and csnE, show high identities to the fourth and fifth CSN subunits of higher eukaryotes. The csnD transcript is abundant during vegetative growth as well as development and the corresponding protein accumulates in the nucleus. Strains deleted for either csn gene are viable and show identical mutant phenotypes at conditions that allow development: hyphae appear partly red and contain cells of reduced size. Additionally, light dependence of propagation onset is affected. The Δcsn mutants are capable of initiating the sexual cycle and develop primordia, but maturation to sexual fruit bodies is blocked. This developmental arrest could not be overcome by overexpression of the sexual activator velvet (VEA). We conclude that the COP9 signalosome in A. nidulans is a key regulator of sexual development, and its proposed structural and functional conservation to the CSN of higher eukaryotes enables studies on this regulatory complex in a genetically amenable organism.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>12864854</pmid><doi>10.1046/j.1365-2958.2003.03612.x</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Aspergillus nidulans - genetics Aspergillus nidulans - growth & development Aspergillus nidulans - radiation effects Aspergillus nidulans - ultrastructure DNA, Complementary - genetics DNA-Binding Proteins - chemistry Fungal Proteins - genetics Fungal Proteins - physiology Gene Expression Regulation, Developmental - radiation effects Gene Expression Regulation, Fungal - radiation effects Genes, Fungal Light Molecular Sequence Data Morphogenesis Multienzyme Complexes - genetics Multienzyme Complexes - physiology Mutagenesis Peptide Hydrolases Phenotype Pigments, Biological - metabolism Protein Structure, Tertiary Recombinant Fusion Proteins - physiology Reproduction Sequence Alignment Sequence Homology, Amino Acid Species Specificity Transcription Factors - chemistry Transcription, Genetic |
| title | The COP9 signalosome is an essential regulator of development in the filamentous fungus Aspergillus nidulans |
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