Experimental Model to Study Reproductive Toxicity of Chemicals using Induced Ovulation in Immature F344 Rats
We investigated a simple method using induced ovulation in immature rats to detect ovarian injury due to chemicals. We investigated the influence of di-(2-ethylhexyl) phthalate (DEHP) on ovulation induced by equine chorionic gonadotropin (eCG) in immature F344 rats. Single injections of 15 and 30 iu...
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| Veröffentlicht in: | Industrial Health 2003, Vol.41(3), pp.287-290 |
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| creator | SEKIGUCHI, Soichiro ITO, Shin HONMA, Takeshi |
| description | We investigated a simple method using induced ovulation in immature rats to detect ovarian injury due to chemicals. We investigated the influence of di-(2-ethylhexyl) phthalate (DEHP) on ovulation induced by equine chorionic gonadotropin (eCG) in immature F344 rats. Single injections of 15 and 30 iu eCG induced ovulation in 4 of 6 rats and in all of 4 rats, respectively. The mean number of ovulated ova was 12.7 in the 15 iu eCG group and 8.0 in the 30 iu group. When rats received 4 daily doses of DEHP at 500 mg/kg, ovulation occurred in 4 of 6 rats in the 15 iu eCG group and in 1 of 3 rats in the 30 iu group. Mean numbers of ovulated ova were 2.50 and 0.33 ova in the 15 and 30 iu groups, respectively. Changes in ovarian and uterine weights were not found. Inhibition of ovulation by the injection of DEHP indicated the utility of induced ovulation in immature rats to detect reproductive toxicity in females. |
| doi_str_mv | 10.2486/indhealth.41.287 |
| format | Article |
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We investigated the influence of di-(2-ethylhexyl) phthalate (DEHP) on ovulation induced by equine chorionic gonadotropin (eCG) in immature F344 rats. Single injections of 15 and 30 iu eCG induced ovulation in 4 of 6 rats and in all of 4 rats, respectively. The mean number of ovulated ova was 12.7 in the 15 iu eCG group and 8.0 in the 30 iu group. When rats received 4 daily doses of DEHP at 500 mg/kg, ovulation occurred in 4 of 6 rats in the 15 iu eCG group and in 1 of 3 rats in the 30 iu group. Mean numbers of ovulated ova were 2.50 and 0.33 ova in the 15 and 30 iu groups, respectively. Changes in ovarian and uterine weights were not found. Inhibition of ovulation by the injection of DEHP indicated the utility of induced ovulation in immature rats to detect reproductive toxicity in females.</description><identifier>ISSN: 0019-8366</identifier><identifier>EISSN: 1880-8026</identifier><identifier>DOI: 10.2486/indhealth.41.287</identifier><identifier>PMID: 12916761</identifier><language>eng</language><publisher>Japan: National Institute of Occupational Safety and Health</publisher><subject>Animals ; Body Weight - drug effects ; Chorionic Gonadotropin ; Di-(2-ethylhexyl) phthalate ; Diethylhexyl Phthalate - toxicity ; eCG ; F344 rats ; Female ; Gonadotropins, Equine ; Immature ; Japan ; Organ Size - drug effects ; Ovary - drug effects ; Ovulation ; Ovulation Induction ; Rats ; Rats, Inbred F344 ; Reproduction - drug effects ; Uterus - drug effects</subject><ispartof>Industrial Health, 2003, Vol.41(3), pp.287-290</ispartof><rights>National Institute of Occupational Safety and Health</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c581t-42a3dffc7302ac4788861f4b4b75851961dbe667c66e0b5267f65a99df6fa2653</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,1877,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12916761$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SEKIGUCHI, Soichiro</creatorcontrib><creatorcontrib>ITO, Shin</creatorcontrib><creatorcontrib>HONMA, Takeshi</creatorcontrib><title>Experimental Model to Study Reproductive Toxicity of Chemicals using Induced Ovulation in Immature F344 Rats</title><title>Industrial Health</title><addtitle>Ind Health</addtitle><description>We investigated a simple method using induced ovulation in immature rats to detect ovarian injury due to chemicals. We investigated the influence of di-(2-ethylhexyl) phthalate (DEHP) on ovulation induced by equine chorionic gonadotropin (eCG) in immature F344 rats. Single injections of 15 and 30 iu eCG induced ovulation in 4 of 6 rats and in all of 4 rats, respectively. The mean number of ovulated ova was 12.7 in the 15 iu eCG group and 8.0 in the 30 iu group. When rats received 4 daily doses of DEHP at 500 mg/kg, ovulation occurred in 4 of 6 rats in the 15 iu eCG group and in 1 of 3 rats in the 30 iu group. Mean numbers of ovulated ova were 2.50 and 0.33 ova in the 15 and 30 iu groups, respectively. Changes in ovarian and uterine weights were not found. Inhibition of ovulation by the injection of DEHP indicated the utility of induced ovulation in immature rats to detect reproductive toxicity in females.</description><subject>Animals</subject><subject>Body Weight - drug effects</subject><subject>Chorionic Gonadotropin</subject><subject>Di-(2-ethylhexyl) phthalate</subject><subject>Diethylhexyl Phthalate - toxicity</subject><subject>eCG</subject><subject>F344 rats</subject><subject>Female</subject><subject>Gonadotropins, Equine</subject><subject>Immature</subject><subject>Japan</subject><subject>Organ Size - drug effects</subject><subject>Ovary - drug effects</subject><subject>Ovulation</subject><subject>Ovulation Induction</subject><subject>Rats</subject><subject>Rats, Inbred F344</subject><subject>Reproduction - drug effects</subject><subject>Uterus - drug effects</subject><issn>0019-8366</issn><issn>1880-8026</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM1P2zAYhy20CTrgzgn5tFs6f8VxjlMFWyUmJD7OlmO_pkZO0sUOov89Rq3Kxb48v0f2g9AVJUsmlPwVBrcBE_NmKeiSqeYELahSpFKEyW9oQQhtK8WlPEM_UnolhMu65afojLKWykbSBYo371uYQg9DNhH_Gx1EnEf8mGe3ww-wnUY32xzeAD-N78GGvMOjx6sN9MGamPCcwvCC10OhwOH7tzmaHMYBhwGv-97keQJ8y4XADyanC_TdlxFcHu5z9Hx787T6W93d_1mvft9VtlY0V4IZ7ry3DSfMWNEopST1ohNdU6uatpK6DqRsrJRAuprJxsvatK3z0hsma36Ofu695fn_Z0hZ9yFZiNEMMM5Jl0aMcsEKSPagncaUJvB6W1qYaacp0Z-F9bGwFlSXwmVyfXDPXQ_ua3BIWoD1HnhN2bzAETBTDjbCl7F8hH9aD0eRHxm7MZOGgX8AwESUVw</recordid><startdate>20030701</startdate><enddate>20030701</enddate><creator>SEKIGUCHI, Soichiro</creator><creator>ITO, Shin</creator><creator>HONMA, Takeshi</creator><general>National Institute of Occupational Safety and Health</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20030701</creationdate><title>Experimental Model to Study Reproductive Toxicity of Chemicals using Induced Ovulation in Immature F344 Rats</title><author>SEKIGUCHI, Soichiro ; ITO, Shin ; HONMA, Takeshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c581t-42a3dffc7302ac4788861f4b4b75851961dbe667c66e0b5267f65a99df6fa2653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Body Weight - drug effects</topic><topic>Chorionic Gonadotropin</topic><topic>Di-(2-ethylhexyl) phthalate</topic><topic>Diethylhexyl Phthalate - toxicity</topic><topic>eCG</topic><topic>F344 rats</topic><topic>Female</topic><topic>Gonadotropins, Equine</topic><topic>Immature</topic><topic>Japan</topic><topic>Organ Size - drug effects</topic><topic>Ovary - drug effects</topic><topic>Ovulation</topic><topic>Ovulation Induction</topic><topic>Rats</topic><topic>Rats, Inbred F344</topic><topic>Reproduction - drug effects</topic><topic>Uterus - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SEKIGUCHI, Soichiro</creatorcontrib><creatorcontrib>ITO, Shin</creatorcontrib><creatorcontrib>HONMA, Takeshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Industrial Health</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SEKIGUCHI, Soichiro</au><au>ITO, Shin</au><au>HONMA, Takeshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Experimental Model to Study Reproductive Toxicity of Chemicals using Induced Ovulation in Immature F344 Rats</atitle><jtitle>Industrial Health</jtitle><addtitle>Ind Health</addtitle><date>2003-07-01</date><risdate>2003</risdate><volume>41</volume><issue>3</issue><spage>287</spage><epage>290</epage><pages>287-290</pages><issn>0019-8366</issn><eissn>1880-8026</eissn><abstract>We investigated a simple method using induced ovulation in immature rats to detect ovarian injury due to chemicals. We investigated the influence of di-(2-ethylhexyl) phthalate (DEHP) on ovulation induced by equine chorionic gonadotropin (eCG) in immature F344 rats. Single injections of 15 and 30 iu eCG induced ovulation in 4 of 6 rats and in all of 4 rats, respectively. The mean number of ovulated ova was 12.7 in the 15 iu eCG group and 8.0 in the 30 iu group. When rats received 4 daily doses of DEHP at 500 mg/kg, ovulation occurred in 4 of 6 rats in the 15 iu eCG group and in 1 of 3 rats in the 30 iu group. Mean numbers of ovulated ova were 2.50 and 0.33 ova in the 15 and 30 iu groups, respectively. Changes in ovarian and uterine weights were not found. Inhibition of ovulation by the injection of DEHP indicated the utility of induced ovulation in immature rats to detect reproductive toxicity in females.</abstract><cop>Japan</cop><pub>National Institute of Occupational Safety and Health</pub><pmid>12916761</pmid><doi>10.2486/indhealth.41.287</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Industrial Health, 2003, Vol.41(3), pp.287-290 |
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| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese |
| subjects | Animals Body Weight - drug effects Chorionic Gonadotropin Di-(2-ethylhexyl) phthalate Diethylhexyl Phthalate - toxicity eCG F344 rats Female Gonadotropins, Equine Immature Japan Organ Size - drug effects Ovary - drug effects Ovulation Ovulation Induction Rats Rats, Inbred F344 Reproduction - drug effects Uterus - drug effects |
| title | Experimental Model to Study Reproductive Toxicity of Chemicals using Induced Ovulation in Immature F344 Rats |
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