Allogeneic islet cells implant on poly- l -lactide matrix to reduce hyperglycaemia in streptozotocin-induced diabetic rat
Abstract Objective To demonstrate the effects of allogeneic islet cell matrix implants for glycaemic control in rats with induced diabetes. Method Sprague-Dawley rats were used as allogeneic donors of islet cells. Cells were seeded on three-dimensional proprietary poly-( l -lactide) matrices. Animal...
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| Veröffentlicht in: | Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] 2017-05, Vol.17 (3), p.411-418 |
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| container_title | Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] |
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| creator | Hendrawan, Siufui, MD, MS Yusuf, Irawan, MD, PhD Hatta, Mochammad, MD, PhD Aman, Makbul, MD Patellongi, Ilhamjaya, MD Serra, Andreas L., MD, MPH Lawrence, Gatot, MD Weber, Ursula Sutedja, Barlian, MD Baer, Hans U., MD |
| description | Abstract Objective To demonstrate the effects of allogeneic islet cell matrix implants for glycaemic control in rats with induced diabetes. Method Sprague-Dawley rats were used as allogeneic donors of islet cells. Cells were seeded on three-dimensional proprietary poly-( l -lactide) matrices. Animals were rendered diabetic and a week later a matrix seeded with islet cells (IMI group) or a control matrix (placebo group) was implanted in the small bowel mesentery. Blood glucose levels were measured weekly for 12 weeks. After sacrifice, implant sections were Gomori stained for beta-cells and immuno-stained for insulin 3, 4, 5, and 6 months post implantation. Results 82% of seeded islet cells attached to the matrices. In the IMI group blood glucose levels were significantly reduced after implantation compared with before implantation across several time points. In the IMI group beta-cells and insulin-positive cells were identified at the implant site. Conclusion The islet cell matrix implant reduced the blood glucose levels although complete normo-glycaemia was not established. The islet cell matrix implant may serve as an additional option for islet cell transplantation using 3D scaffold platforms for better survival and function of the islet cells. |
| doi_str_mv | 10.1016/j.pan.2017.02.017 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1881770723</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S142439031730042X</els_id><sourcerecordid>1902501712</sourcerecordid><originalsourceid>FETCH-LOGICAL-c436t-b51e9626397074cc46d47c802b89c0943190e57c23c77811aa0fee3ffab1ca4c3</originalsourceid><addsrcrecordid>eNp9kktv1TAQhSNERR_wA9ggS2zYJPh18xASUlW1gFSJBSCxs5zJpPji2MF2EOmvr6NbitQFq_HiO2c8c6YoXjJaMcrqt_tq1q7ilDUV5VUuT4oTJrksRcfY04c3FcfFaYx7SjlnrHtWHPNWyKaW9Umxnlvrb9ChAWKixUQArY3ETLPVLhHvyOztWhJLSqshmQHJpFMwf0jyJOCwAJIf64zhxq6gcTKaGEdiCjgnf-uTB-NK4zZuIIPRPabcKuj0vDgatY344r6eFd-uLr9efCyvP3_4dHF-XYIUdSr7HcOu5rXoGtpIAFkPsoGW8r7tgHZSsI7irgEuoGlaxrSmI6IYR90z0BLEWfHm4DsH_2vBmNRk4jakduiXqFjbsiZ7c5HR14_QvV-Cy79TuQvf5Q0znil2oCD4GAOOag5m0mFVjKotF7VXORe15aIoV7lkzat756WfcHhQ_A0iA-8OAOZV_DYYVASDLi_NBISkBm_-a__-kRqscQa0_Ykrxn9TqJgF6st2GNtdZCmlkn8Xd0tCs0A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1902501712</pqid></control><display><type>article</type><title>Allogeneic islet cells implant on poly- l -lactide matrix to reduce hyperglycaemia in streptozotocin-induced diabetic rat</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Hendrawan, Siufui, MD, MS ; Yusuf, Irawan, MD, PhD ; Hatta, Mochammad, MD, PhD ; Aman, Makbul, MD ; Patellongi, Ilhamjaya, MD ; Serra, Andreas L., MD, MPH ; Lawrence, Gatot, MD ; Weber, Ursula ; Sutedja, Barlian, MD ; Baer, Hans U., MD</creator><creatorcontrib>Hendrawan, Siufui, MD, MS ; Yusuf, Irawan, MD, PhD ; Hatta, Mochammad, MD, PhD ; Aman, Makbul, MD ; Patellongi, Ilhamjaya, MD ; Serra, Andreas L., MD, MPH ; Lawrence, Gatot, MD ; Weber, Ursula ; Sutedja, Barlian, MD ; Baer, Hans U., MD</creatorcontrib><description>Abstract Objective To demonstrate the effects of allogeneic islet cell matrix implants for glycaemic control in rats with induced diabetes. Method Sprague-Dawley rats were used as allogeneic donors of islet cells. Cells were seeded on three-dimensional proprietary poly-( l -lactide) matrices. Animals were rendered diabetic and a week later a matrix seeded with islet cells (IMI group) or a control matrix (placebo group) was implanted in the small bowel mesentery. Blood glucose levels were measured weekly for 12 weeks. After sacrifice, implant sections were Gomori stained for beta-cells and immuno-stained for insulin 3, 4, 5, and 6 months post implantation. Results 82% of seeded islet cells attached to the matrices. In the IMI group blood glucose levels were significantly reduced after implantation compared with before implantation across several time points. In the IMI group beta-cells and insulin-positive cells were identified at the implant site. Conclusion The islet cell matrix implant reduced the blood glucose levels although complete normo-glycaemia was not established. The islet cell matrix implant may serve as an additional option for islet cell transplantation using 3D scaffold platforms for better survival and function of the islet cells.</description><identifier>ISSN: 1424-3903</identifier><identifier>EISSN: 1424-3911</identifier><identifier>DOI: 10.1016/j.pan.2017.02.017</identifier><identifier>PMID: 28347646</identifier><language>eng</language><publisher>Switzerland: Elsevier B.V</publisher><subject>Acids ; Animals ; Beta cells ; Biomedical materials ; Blood glucose ; Blood Glucose - metabolism ; Body Weight ; Cell adhesion & migration ; Cell Culture Techniques ; Cell division ; Cell Separation ; Cell Survival ; Diabetes ; Diabetes mellitus ; Diabetes Mellitus, Experimental - complications ; Diabetes Mellitus, Experimental - pathology ; Diabetes Mellitus, Experimental - therapy ; Endocrinology & Metabolism ; Gastroenterology and Hepatology ; Glycaemic control ; Hyperglycemia ; Hyperglycemia - etiology ; Hyperglycemia - therapy ; Insulin ; Insulin - biosynthesis ; Islet cells ; Islet matrix implant ; Islets of Langerhans - metabolism ; Islets of Langerhans Transplantation - methods ; Liver ; Male ; Mesentery ; Mortality ; Pancreas ; Pancreatic islet transplantation ; Polyesters ; Rat ; Rats ; Rats, Sprague-Dawley ; Rodents ; Small intestine ; Streptozocin ; Transplantation ; Transplants & implants</subject><ispartof>Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], 2017-05, Vol.17 (3), p.411-418</ispartof><rights>IAP and EPC</rights><rights>2017 IAP and EPC</rights><rights>Copyright © 2017 IAP and EPC. Published by Elsevier B.V. All rights reserved.</rights><rights>2017. IAP and EPC</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-b51e9626397074cc46d47c802b89c0943190e57c23c77811aa0fee3ffab1ca4c3</citedby><cites>FETCH-LOGICAL-c436t-b51e9626397074cc46d47c802b89c0943190e57c23c77811aa0fee3ffab1ca4c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28347646$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hendrawan, Siufui, MD, MS</creatorcontrib><creatorcontrib>Yusuf, Irawan, MD, PhD</creatorcontrib><creatorcontrib>Hatta, Mochammad, MD, PhD</creatorcontrib><creatorcontrib>Aman, Makbul, MD</creatorcontrib><creatorcontrib>Patellongi, Ilhamjaya, MD</creatorcontrib><creatorcontrib>Serra, Andreas L., MD, MPH</creatorcontrib><creatorcontrib>Lawrence, Gatot, MD</creatorcontrib><creatorcontrib>Weber, Ursula</creatorcontrib><creatorcontrib>Sutedja, Barlian, MD</creatorcontrib><creatorcontrib>Baer, Hans U., MD</creatorcontrib><title>Allogeneic islet cells implant on poly- l -lactide matrix to reduce hyperglycaemia in streptozotocin-induced diabetic rat</title><title>Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]</title><addtitle>Pancreatology</addtitle><description>Abstract Objective To demonstrate the effects of allogeneic islet cell matrix implants for glycaemic control in rats with induced diabetes. Method Sprague-Dawley rats were used as allogeneic donors of islet cells. Cells were seeded on three-dimensional proprietary poly-( l -lactide) matrices. Animals were rendered diabetic and a week later a matrix seeded with islet cells (IMI group) or a control matrix (placebo group) was implanted in the small bowel mesentery. Blood glucose levels were measured weekly for 12 weeks. After sacrifice, implant sections were Gomori stained for beta-cells and immuno-stained for insulin 3, 4, 5, and 6 months post implantation. Results 82% of seeded islet cells attached to the matrices. In the IMI group blood glucose levels were significantly reduced after implantation compared with before implantation across several time points. In the IMI group beta-cells and insulin-positive cells were identified at the implant site. Conclusion The islet cell matrix implant reduced the blood glucose levels although complete normo-glycaemia was not established. The islet cell matrix implant may serve as an additional option for islet cell transplantation using 3D scaffold platforms for better survival and function of the islet cells.</description><subject>Acids</subject><subject>Animals</subject><subject>Beta cells</subject><subject>Biomedical materials</subject><subject>Blood glucose</subject><subject>Blood Glucose - metabolism</subject><subject>Body Weight</subject><subject>Cell adhesion & migration</subject><subject>Cell Culture Techniques</subject><subject>Cell division</subject><subject>Cell Separation</subject><subject>Cell Survival</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Diabetes Mellitus, Experimental - complications</subject><subject>Diabetes Mellitus, Experimental - pathology</subject><subject>Diabetes Mellitus, Experimental - therapy</subject><subject>Endocrinology & Metabolism</subject><subject>Gastroenterology and Hepatology</subject><subject>Glycaemic control</subject><subject>Hyperglycemia</subject><subject>Hyperglycemia - etiology</subject><subject>Hyperglycemia - therapy</subject><subject>Insulin</subject><subject>Insulin - biosynthesis</subject><subject>Islet cells</subject><subject>Islet matrix implant</subject><subject>Islets of Langerhans - metabolism</subject><subject>Islets of Langerhans Transplantation - methods</subject><subject>Liver</subject><subject>Male</subject><subject>Mesentery</subject><subject>Mortality</subject><subject>Pancreas</subject><subject>Pancreatic islet transplantation</subject><subject>Polyesters</subject><subject>Rat</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Rodents</subject><subject>Small intestine</subject><subject>Streptozocin</subject><subject>Transplantation</subject><subject>Transplants & implants</subject><issn>1424-3903</issn><issn>1424-3911</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kktv1TAQhSNERR_wA9ggS2zYJPh18xASUlW1gFSJBSCxs5zJpPji2MF2EOmvr6NbitQFq_HiO2c8c6YoXjJaMcrqt_tq1q7ilDUV5VUuT4oTJrksRcfY04c3FcfFaYx7SjlnrHtWHPNWyKaW9Umxnlvrb9ChAWKixUQArY3ETLPVLhHvyOztWhJLSqshmQHJpFMwf0jyJOCwAJIf64zhxq6gcTKaGEdiCjgnf-uTB-NK4zZuIIPRPabcKuj0vDgatY344r6eFd-uLr9efCyvP3_4dHF-XYIUdSr7HcOu5rXoGtpIAFkPsoGW8r7tgHZSsI7irgEuoGlaxrSmI6IYR90z0BLEWfHm4DsH_2vBmNRk4jakduiXqFjbsiZ7c5HR14_QvV-Cy79TuQvf5Q0znil2oCD4GAOOag5m0mFVjKotF7VXORe15aIoV7lkzat756WfcHhQ_A0iA-8OAOZV_DYYVASDLi_NBISkBm_-a__-kRqscQa0_Ykrxn9TqJgF6st2GNtdZCmlkn8Xd0tCs0A</recordid><startdate>20170501</startdate><enddate>20170501</enddate><creator>Hendrawan, Siufui, MD, MS</creator><creator>Yusuf, Irawan, MD, PhD</creator><creator>Hatta, Mochammad, MD, PhD</creator><creator>Aman, Makbul, MD</creator><creator>Patellongi, Ilhamjaya, MD</creator><creator>Serra, Andreas L., MD, MPH</creator><creator>Lawrence, Gatot, MD</creator><creator>Weber, Ursula</creator><creator>Sutedja, Barlian, MD</creator><creator>Baer, Hans U., MD</creator><general>Elsevier B.V</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>20170501</creationdate><title>Allogeneic islet cells implant on poly- l -lactide matrix to reduce hyperglycaemia in streptozotocin-induced diabetic rat</title><author>Hendrawan, Siufui, MD, MS ; Yusuf, Irawan, MD, PhD ; Hatta, Mochammad, MD, PhD ; Aman, Makbul, MD ; Patellongi, Ilhamjaya, MD ; Serra, Andreas L., MD, MPH ; Lawrence, Gatot, MD ; Weber, Ursula ; Sutedja, Barlian, MD ; Baer, Hans U., MD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-b51e9626397074cc46d47c802b89c0943190e57c23c77811aa0fee3ffab1ca4c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Acids</topic><topic>Animals</topic><topic>Beta cells</topic><topic>Biomedical materials</topic><topic>Blood glucose</topic><topic>Blood Glucose - metabolism</topic><topic>Body Weight</topic><topic>Cell adhesion & migration</topic><topic>Cell Culture Techniques</topic><topic>Cell division</topic><topic>Cell Separation</topic><topic>Cell Survival</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Diabetes Mellitus, Experimental - complications</topic><topic>Diabetes Mellitus, Experimental - pathology</topic><topic>Diabetes Mellitus, Experimental - therapy</topic><topic>Endocrinology & Metabolism</topic><topic>Gastroenterology and Hepatology</topic><topic>Glycaemic control</topic><topic>Hyperglycemia</topic><topic>Hyperglycemia - etiology</topic><topic>Hyperglycemia - therapy</topic><topic>Insulin</topic><topic>Insulin - biosynthesis</topic><topic>Islet cells</topic><topic>Islet matrix implant</topic><topic>Islets of Langerhans - metabolism</topic><topic>Islets of Langerhans Transplantation - methods</topic><topic>Liver</topic><topic>Male</topic><topic>Mesentery</topic><topic>Mortality</topic><topic>Pancreas</topic><topic>Pancreatic islet transplantation</topic><topic>Polyesters</topic><topic>Rat</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Rodents</topic><topic>Small intestine</topic><topic>Streptozocin</topic><topic>Transplantation</topic><topic>Transplants & implants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hendrawan, Siufui, MD, MS</creatorcontrib><creatorcontrib>Yusuf, Irawan, MD, PhD</creatorcontrib><creatorcontrib>Hatta, Mochammad, MD, PhD</creatorcontrib><creatorcontrib>Aman, Makbul, MD</creatorcontrib><creatorcontrib>Patellongi, Ilhamjaya, MD</creatorcontrib><creatorcontrib>Serra, Andreas L., MD, MPH</creatorcontrib><creatorcontrib>Lawrence, Gatot, MD</creatorcontrib><creatorcontrib>Weber, Ursula</creatorcontrib><creatorcontrib>Sutedja, Barlian, MD</creatorcontrib><creatorcontrib>Baer, Hans U., MD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hendrawan, Siufui, MD, MS</au><au>Yusuf, Irawan, MD, PhD</au><au>Hatta, Mochammad, MD, PhD</au><au>Aman, Makbul, MD</au><au>Patellongi, Ilhamjaya, MD</au><au>Serra, Andreas L., MD, MPH</au><au>Lawrence, Gatot, MD</au><au>Weber, Ursula</au><au>Sutedja, Barlian, MD</au><au>Baer, Hans U., MD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Allogeneic islet cells implant on poly- l -lactide matrix to reduce hyperglycaemia in streptozotocin-induced diabetic rat</atitle><jtitle>Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]</jtitle><addtitle>Pancreatology</addtitle><date>2017-05-01</date><risdate>2017</risdate><volume>17</volume><issue>3</issue><spage>411</spage><epage>418</epage><pages>411-418</pages><issn>1424-3903</issn><eissn>1424-3911</eissn><abstract>Abstract Objective To demonstrate the effects of allogeneic islet cell matrix implants for glycaemic control in rats with induced diabetes. Method Sprague-Dawley rats were used as allogeneic donors of islet cells. Cells were seeded on three-dimensional proprietary poly-( l -lactide) matrices. Animals were rendered diabetic and a week later a matrix seeded with islet cells (IMI group) or a control matrix (placebo group) was implanted in the small bowel mesentery. Blood glucose levels were measured weekly for 12 weeks. After sacrifice, implant sections were Gomori stained for beta-cells and immuno-stained for insulin 3, 4, 5, and 6 months post implantation. Results 82% of seeded islet cells attached to the matrices. In the IMI group blood glucose levels were significantly reduced after implantation compared with before implantation across several time points. In the IMI group beta-cells and insulin-positive cells were identified at the implant site. Conclusion The islet cell matrix implant reduced the blood glucose levels although complete normo-glycaemia was not established. The islet cell matrix implant may serve as an additional option for islet cell transplantation using 3D scaffold platforms for better survival and function of the islet cells.</abstract><cop>Switzerland</cop><pub>Elsevier B.V</pub><pmid>28347646</pmid><doi>10.1016/j.pan.2017.02.017</doi><tpages>8</tpages></addata></record> |
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| ispartof | Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], 2017-05, Vol.17 (3), p.411-418 |
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| subjects | Acids Animals Beta cells Biomedical materials Blood glucose Blood Glucose - metabolism Body Weight Cell adhesion & migration Cell Culture Techniques Cell division Cell Separation Cell Survival Diabetes Diabetes mellitus Diabetes Mellitus, Experimental - complications Diabetes Mellitus, Experimental - pathology Diabetes Mellitus, Experimental - therapy Endocrinology & Metabolism Gastroenterology and Hepatology Glycaemic control Hyperglycemia Hyperglycemia - etiology Hyperglycemia - therapy Insulin Insulin - biosynthesis Islet cells Islet matrix implant Islets of Langerhans - metabolism Islets of Langerhans Transplantation - methods Liver Male Mesentery Mortality Pancreas Pancreatic islet transplantation Polyesters Rat Rats Rats, Sprague-Dawley Rodents Small intestine Streptozocin Transplantation Transplants & implants |
| title | Allogeneic islet cells implant on poly- l -lactide matrix to reduce hyperglycaemia in streptozotocin-induced diabetic rat |
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