Role of IL-13 Genetic Variants in Signalling of Asthma
Asthma is a chronic inflammatory disease of the lower airways characterised by intermittent airway narrowing and airflow obstruction. The aim of this study was to examine the association of IL-13 Arg 130 Gln (A/G) and -1112C/T cytokine gene polymorphisms and to know the secretion of IL-13 cytokine l...
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| Veröffentlicht in: | Inflammation 2017-04, Vol.40 (2), p.566-577 |
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| creator | Alasandagutti, Madhavi Latha Ansari, Mohd Soheb Sadat Sagurthi, S. R. Valluri, Vijayalakshmi Gaddam, Sumanlatha |
| description | Asthma is a chronic inflammatory disease of the lower airways characterised by intermittent airway narrowing and airflow obstruction. The aim of this study was to examine the association of IL-13 Arg 130 Gln (A/G) and -1112C/T cytokine gene polymorphisms and to know the secretion of IL-13 cytokine levels and the interactions between the IL-13 130A/G and IL-13Rα1/IL-4Rα complex cytokine genes. The study population comprised of atopic and non-atopic asthma patients and healthy controls (HC) (
N
= 120). Single nucleotide polymorphisms (SNPs) were determined by restriction fragment length polymorphism (RFLP). IL-13 cytokine serum levels were measured by enzyme-linked immunosorbent assay (ELISA), and homology modelling of IL-13 A/G cytokine gene was performed through
in silico
analysis. In IL-13 130A/G cytokine gene AG, GG genotypes (
p
|
| doi_str_mv | 10.1007/s10753-016-0503-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1881765464</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1881765464</sourcerecordid><originalsourceid>FETCH-LOGICAL-c405t-24c7e6fd8b7906502e7c01caaabd93086c44021cc2b29296df43dc775915a203</originalsourceid><addsrcrecordid>eNqNkE1Lw0AQhhdRbK3-AC8S8OJldXY3-3UsRWuhIGjxumw2m5qSj5pNDv57E1JFBMHTHOZ532EehC4J3BIAeRcISM4wEIGBA8PsCE0JlwxTLsUxmgITgJnWcoLOQtgBgNKKnaIJVaCYFGKKxHNd-KjOotUaExYtfeXb3EWvtslt1YYor6KXfFvZosir7cDNQ_tW2nN0ktki-IvDnKHNw_1m8YjXT8vVYr7GLgbeYho76UWWqkRqEByolw6Is9YmqWaghItjoMQ5mlBNtUizmKVOSq4JtxTYDN2Mtfumfu98aE2ZB-eLwla-7oIhShEpeCzif6CCxAK4Uj16_Qvd1V3T_zhQUjPJRC9xhshIuaYOofGZ2Td5aZsPQ8AM-s2o3_T6zaDfsD5zdWjuktKn34kv3z1ARyD0q2rrmx-n_2z9BDSDi1c</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1879373657</pqid></control><display><type>article</type><title>Role of IL-13 Genetic Variants in Signalling of Asthma</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Alasandagutti, Madhavi Latha ; Ansari, Mohd Soheb Sadat ; Sagurthi, S. R. ; Valluri, Vijayalakshmi ; Gaddam, Sumanlatha</creator><creatorcontrib>Alasandagutti, Madhavi Latha ; Ansari, Mohd Soheb Sadat ; Sagurthi, S. R. ; Valluri, Vijayalakshmi ; Gaddam, Sumanlatha</creatorcontrib><description>Asthma is a chronic inflammatory disease of the lower airways characterised by intermittent airway narrowing and airflow obstruction. The aim of this study was to examine the association of IL-13 Arg 130 Gln (A/G) and -1112C/T cytokine gene polymorphisms and to know the secretion of IL-13 cytokine levels and the interactions between the IL-13 130A/G and IL-13Rα1/IL-4Rα complex cytokine genes. The study population comprised of atopic and non-atopic asthma patients and healthy controls (HC) (
N
= 120). Single nucleotide polymorphisms (SNPs) were determined by restriction fragment length polymorphism (RFLP). IL-13 cytokine serum levels were measured by enzyme-linked immunosorbent assay (ELISA), and homology modelling of IL-13 A/G cytokine gene was performed through
in silico
analysis. In IL-13 130A/G cytokine gene AG, GG genotypes (
p
< 0.0042, OR = 2.87, CI 1.46–5.65; OR = 1.92, CI 1.06–3.48) were found to be significant in atopic asthma patients
vs
HC. The mean IL-13 serum cytokine levels were found to be significantly high in atopic (38.48 ± 36.54) and non-atopic (36.05 ± 34.54) asthma patients whereas total serum IgE levels were significantly high at
p
< 0.0001 in atopic and low in non-atopic asthma patients at
p
< 0.003 compared to HC.
In silico
analysis indicated that residue IL-13 130 with charge modifying variants was crucial in ligand-receptor interactions. IL-13 cytokine serum levels were significantly high in atopic and non-atopic asthma patients compared to HC. The GG genotype of IL-13 130A/G cytokine gene might be involved in the induced production of total IgE and IL-13 cytokine serum levels suggesting IL-13 may be important in the signalling of asthma.</description><identifier>ISSN: 0360-3997</identifier><identifier>EISSN: 1573-2576</identifier><identifier>DOI: 10.1007/s10753-016-0503-3</identifier><identifier>PMID: 28083766</identifier><identifier>CODEN: INFLD4</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adult ; Asthma - genetics ; Asthma - metabolism ; Biomedical and Life Sciences ; Biomedicine ; Case-Control Studies ; Female ; Genotype ; Humans ; Hypersensitivity, Immediate - blood ; Hypersensitivity, Immediate - genetics ; Immunoglobulin E - blood ; Immunology ; Interleukin-13 - blood ; Interleukin-13 - genetics ; Interleukin-13 - physiology ; Internal Medicine ; Male ; Original Article ; Pathology ; Pharmacology/Toxicology ; Polymorphism, Single Nucleotide - genetics ; Rheumatology ; Signal Transduction</subject><ispartof>Inflammation, 2017-04, Vol.40 (2), p.566-577</ispartof><rights>Springer Science+Business Media New York 2017</rights><rights>Inflammation is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-24c7e6fd8b7906502e7c01caaabd93086c44021cc2b29296df43dc775915a203</citedby><cites>FETCH-LOGICAL-c405t-24c7e6fd8b7906502e7c01caaabd93086c44021cc2b29296df43dc775915a203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10753-016-0503-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10753-016-0503-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28083766$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alasandagutti, Madhavi Latha</creatorcontrib><creatorcontrib>Ansari, Mohd Soheb Sadat</creatorcontrib><creatorcontrib>Sagurthi, S. R.</creatorcontrib><creatorcontrib>Valluri, Vijayalakshmi</creatorcontrib><creatorcontrib>Gaddam, Sumanlatha</creatorcontrib><title>Role of IL-13 Genetic Variants in Signalling of Asthma</title><title>Inflammation</title><addtitle>Inflammation</addtitle><addtitle>Inflammation</addtitle><description>Asthma is a chronic inflammatory disease of the lower airways characterised by intermittent airway narrowing and airflow obstruction. The aim of this study was to examine the association of IL-13 Arg 130 Gln (A/G) and -1112C/T cytokine gene polymorphisms and to know the secretion of IL-13 cytokine levels and the interactions between the IL-13 130A/G and IL-13Rα1/IL-4Rα complex cytokine genes. The study population comprised of atopic and non-atopic asthma patients and healthy controls (HC) (
N
= 120). Single nucleotide polymorphisms (SNPs) were determined by restriction fragment length polymorphism (RFLP). IL-13 cytokine serum levels were measured by enzyme-linked immunosorbent assay (ELISA), and homology modelling of IL-13 A/G cytokine gene was performed through
in silico
analysis. In IL-13 130A/G cytokine gene AG, GG genotypes (
p
< 0.0042, OR = 2.87, CI 1.46–5.65; OR = 1.92, CI 1.06–3.48) were found to be significant in atopic asthma patients
vs
HC. The mean IL-13 serum cytokine levels were found to be significantly high in atopic (38.48 ± 36.54) and non-atopic (36.05 ± 34.54) asthma patients whereas total serum IgE levels were significantly high at
p
< 0.0001 in atopic and low in non-atopic asthma patients at
p
< 0.003 compared to HC.
In silico
analysis indicated that residue IL-13 130 with charge modifying variants was crucial in ligand-receptor interactions. IL-13 cytokine serum levels were significantly high in atopic and non-atopic asthma patients compared to HC. The GG genotype of IL-13 130A/G cytokine gene might be involved in the induced production of total IgE and IL-13 cytokine serum levels suggesting IL-13 may be important in the signalling of asthma.</description><subject>Adult</subject><subject>Asthma - genetics</subject><subject>Asthma - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Case-Control Studies</subject><subject>Female</subject><subject>Genotype</subject><subject>Humans</subject><subject>Hypersensitivity, Immediate - blood</subject><subject>Hypersensitivity, Immediate - genetics</subject><subject>Immunoglobulin E - blood</subject><subject>Immunology</subject><subject>Interleukin-13 - blood</subject><subject>Interleukin-13 - genetics</subject><subject>Interleukin-13 - physiology</subject><subject>Internal Medicine</subject><subject>Male</subject><subject>Original Article</subject><subject>Pathology</subject><subject>Pharmacology/Toxicology</subject><subject>Polymorphism, Single Nucleotide - genetics</subject><subject>Rheumatology</subject><subject>Signal Transduction</subject><issn>0360-3997</issn><issn>1573-2576</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkE1Lw0AQhhdRbK3-AC8S8OJldXY3-3UsRWuhIGjxumw2m5qSj5pNDv57E1JFBMHTHOZ532EehC4J3BIAeRcISM4wEIGBA8PsCE0JlwxTLsUxmgITgJnWcoLOQtgBgNKKnaIJVaCYFGKKxHNd-KjOotUaExYtfeXb3EWvtslt1YYor6KXfFvZosir7cDNQ_tW2nN0ktki-IvDnKHNw_1m8YjXT8vVYr7GLgbeYho76UWWqkRqEByolw6Is9YmqWaghItjoMQ5mlBNtUizmKVOSq4JtxTYDN2Mtfumfu98aE2ZB-eLwla-7oIhShEpeCzif6CCxAK4Uj16_Qvd1V3T_zhQUjPJRC9xhshIuaYOofGZ2Td5aZsPQ8AM-s2o3_T6zaDfsD5zdWjuktKn34kv3z1ARyD0q2rrmx-n_2z9BDSDi1c</recordid><startdate>20170401</startdate><enddate>20170401</enddate><creator>Alasandagutti, Madhavi Latha</creator><creator>Ansari, Mohd Soheb Sadat</creator><creator>Sagurthi, S. R.</creator><creator>Valluri, Vijayalakshmi</creator><creator>Gaddam, Sumanlatha</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20170401</creationdate><title>Role of IL-13 Genetic Variants in Signalling of Asthma</title><author>Alasandagutti, Madhavi Latha ; Ansari, Mohd Soheb Sadat ; Sagurthi, S. R. ; Valluri, Vijayalakshmi ; Gaddam, Sumanlatha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-24c7e6fd8b7906502e7c01caaabd93086c44021cc2b29296df43dc775915a203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>Asthma - genetics</topic><topic>Asthma - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Case-Control Studies</topic><topic>Female</topic><topic>Genotype</topic><topic>Humans</topic><topic>Hypersensitivity, Immediate - blood</topic><topic>Hypersensitivity, Immediate - genetics</topic><topic>Immunoglobulin E - blood</topic><topic>Immunology</topic><topic>Interleukin-13 - blood</topic><topic>Interleukin-13 - genetics</topic><topic>Interleukin-13 - physiology</topic><topic>Internal Medicine</topic><topic>Male</topic><topic>Original Article</topic><topic>Pathology</topic><topic>Pharmacology/Toxicology</topic><topic>Polymorphism, Single Nucleotide - genetics</topic><topic>Rheumatology</topic><topic>Signal Transduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alasandagutti, Madhavi Latha</creatorcontrib><creatorcontrib>Ansari, Mohd Soheb Sadat</creatorcontrib><creatorcontrib>Sagurthi, S. R.</creatorcontrib><creatorcontrib>Valluri, Vijayalakshmi</creatorcontrib><creatorcontrib>Gaddam, Sumanlatha</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Inflammation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alasandagutti, Madhavi Latha</au><au>Ansari, Mohd Soheb Sadat</au><au>Sagurthi, S. R.</au><au>Valluri, Vijayalakshmi</au><au>Gaddam, Sumanlatha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of IL-13 Genetic Variants in Signalling of Asthma</atitle><jtitle>Inflammation</jtitle><stitle>Inflammation</stitle><addtitle>Inflammation</addtitle><date>2017-04-01</date><risdate>2017</risdate><volume>40</volume><issue>2</issue><spage>566</spage><epage>577</epage><pages>566-577</pages><issn>0360-3997</issn><eissn>1573-2576</eissn><coden>INFLD4</coden><abstract>Asthma is a chronic inflammatory disease of the lower airways characterised by intermittent airway narrowing and airflow obstruction. The aim of this study was to examine the association of IL-13 Arg 130 Gln (A/G) and -1112C/T cytokine gene polymorphisms and to know the secretion of IL-13 cytokine levels and the interactions between the IL-13 130A/G and IL-13Rα1/IL-4Rα complex cytokine genes. The study population comprised of atopic and non-atopic asthma patients and healthy controls (HC) (
N
= 120). Single nucleotide polymorphisms (SNPs) were determined by restriction fragment length polymorphism (RFLP). IL-13 cytokine serum levels were measured by enzyme-linked immunosorbent assay (ELISA), and homology modelling of IL-13 A/G cytokine gene was performed through
in silico
analysis. In IL-13 130A/G cytokine gene AG, GG genotypes (
p
< 0.0042, OR = 2.87, CI 1.46–5.65; OR = 1.92, CI 1.06–3.48) were found to be significant in atopic asthma patients
vs
HC. The mean IL-13 serum cytokine levels were found to be significantly high in atopic (38.48 ± 36.54) and non-atopic (36.05 ± 34.54) asthma patients whereas total serum IgE levels were significantly high at
p
< 0.0001 in atopic and low in non-atopic asthma patients at
p
< 0.003 compared to HC.
In silico
analysis indicated that residue IL-13 130 with charge modifying variants was crucial in ligand-receptor interactions. IL-13 cytokine serum levels were significantly high in atopic and non-atopic asthma patients compared to HC. The GG genotype of IL-13 130A/G cytokine gene might be involved in the induced production of total IgE and IL-13 cytokine serum levels suggesting IL-13 may be important in the signalling of asthma.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>28083766</pmid><doi>10.1007/s10753-016-0503-3</doi><tpages>12</tpages></addata></record> |
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| subjects | Adult Asthma - genetics Asthma - metabolism Biomedical and Life Sciences Biomedicine Case-Control Studies Female Genotype Humans Hypersensitivity, Immediate - blood Hypersensitivity, Immediate - genetics Immunoglobulin E - blood Immunology Interleukin-13 - blood Interleukin-13 - genetics Interleukin-13 - physiology Internal Medicine Male Original Article Pathology Pharmacology/Toxicology Polymorphism, Single Nucleotide - genetics Rheumatology Signal Transduction |
| title | Role of IL-13 Genetic Variants in Signalling of Asthma |
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