Polygala tenuifolia polysaccharide (PTP) inhibits cell proliferation by repressing Bmi-1 expression and downregulating telomerase activity
In our previous study, we isolated a homogeneous polysaccharide (PTP) with antitumor activity from the roots of Polygala tenuifolia . In view of the close correlation between Bmi-1 expression and progression of ovarian cancer, we intend to elucidate the mechanism of its activity by determining the B...
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| Veröffentlicht in: | Tumor biology 2015-04, Vol.36 (4), p.2907-2912 |
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| creator | Zhang, Fubin Song, Xiaowei Li, Li Wang, Jingfang Lin, Leyuan Li, Cong Li, Hongtao Lv, Yanju Jin, Yinghua Liu, Ying Hu, Yu Xin, Tao |
| description | In our previous study, we isolated a homogeneous polysaccharide (PTP) with antitumor activity from the roots of
Polygala tenuifolia
. In view of the close correlation between Bmi-1 expression and progression of ovarian cancer, we intend to elucidate the mechanism of its activity by determining the Bmi-1 expression and the telomerase activity in human ovarian carcinoma OVCAR-3 cells following treatment with PTP at three concentrations of 0.5, 1, and 2 mg/mL for 48 h. MTT and colony-forming assays revealed that PTP had a significant inhibitory effect on the cell growth and colony formation of OVCAR-3 cells. Furthermore, Western blot and real-time PCR analysis showed that PTP inhibited Bmi-1 both in protein and transcript levels. Besides, the telomerase activity in OVCAR-3 cells was also downregulated after PTP treatment for 48 h. Taken together, the inhibitory effect of PTP on the cell growth was at least in part mediated via the downregulation of Bmi-1 expression and the telomerase activity in OVCAR-3 cells, and PTP might be a new candidate for chemotherapeutic agent against human ovarian cancer. |
| doi_str_mv | 10.1007/s13277-014-2920-y |
| format | Article |
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Polygala tenuifolia
. In view of the close correlation between Bmi-1 expression and progression of ovarian cancer, we intend to elucidate the mechanism of its activity by determining the Bmi-1 expression and the telomerase activity in human ovarian carcinoma OVCAR-3 cells following treatment with PTP at three concentrations of 0.5, 1, and 2 mg/mL for 48 h. MTT and colony-forming assays revealed that PTP had a significant inhibitory effect on the cell growth and colony formation of OVCAR-3 cells. Furthermore, Western blot and real-time PCR analysis showed that PTP inhibited Bmi-1 both in protein and transcript levels. Besides, the telomerase activity in OVCAR-3 cells was also downregulated after PTP treatment for 48 h. Taken together, the inhibitory effect of PTP on the cell growth was at least in part mediated via the downregulation of Bmi-1 expression and the telomerase activity in OVCAR-3 cells, and PTP might be a new candidate for chemotherapeutic agent against human ovarian cancer.</description><identifier>ISSN: 1010-4283</identifier><identifier>EISSN: 1423-0380</identifier><identifier>DOI: 10.1007/s13277-014-2920-y</identifier><identifier>PMID: 25501509</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Biomedical and Life Sciences ; Biomedicine ; Cancer Research ; Cell Cycle - drug effects ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Cellular biology ; Enzymes ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Ovarian cancer ; Ovarian Neoplasms - drug therapy ; Ovarian Neoplasms - genetics ; Ovarian Neoplasms - pathology ; Polycomb Repressive Complex 1 - biosynthesis ; Polygala ; Polygala - chemistry ; Polysaccharides - administration & dosage ; Polysaccharides - chemistry ; Research Article ; Telomerase ; Telomerase - biosynthesis ; Telomerase - genetics</subject><ispartof>Tumor biology, 2015-04, Vol.36 (4), p.2907-2912</ispartof><rights>International Society of Oncology and BioMarkers (ISOBM) 2014</rights><rights>International Society of Oncology and BioMarkers (ISOBM) 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-6a962ac59cda945f4073f0f85a5c319b3147bacf61187aa75ba68f39ea0ff5aa3</citedby><cites>FETCH-LOGICAL-c438t-6a962ac59cda945f4073f0f85a5c319b3147bacf61187aa75ba68f39ea0ff5aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13277-014-2920-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13277-014-2920-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25501509$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Fubin</creatorcontrib><creatorcontrib>Song, Xiaowei</creatorcontrib><creatorcontrib>Li, Li</creatorcontrib><creatorcontrib>Wang, Jingfang</creatorcontrib><creatorcontrib>Lin, Leyuan</creatorcontrib><creatorcontrib>Li, Cong</creatorcontrib><creatorcontrib>Li, Hongtao</creatorcontrib><creatorcontrib>Lv, Yanju</creatorcontrib><creatorcontrib>Jin, Yinghua</creatorcontrib><creatorcontrib>Liu, Ying</creatorcontrib><creatorcontrib>Hu, Yu</creatorcontrib><creatorcontrib>Xin, Tao</creatorcontrib><title>Polygala tenuifolia polysaccharide (PTP) inhibits cell proliferation by repressing Bmi-1 expression and downregulating telomerase activity</title><title>Tumor biology</title><addtitle>Tumor Biol</addtitle><addtitle>Tumour Biol</addtitle><description>In our previous study, we isolated a homogeneous polysaccharide (PTP) with antitumor activity from the roots of
Polygala tenuifolia
. In view of the close correlation between Bmi-1 expression and progression of ovarian cancer, we intend to elucidate the mechanism of its activity by determining the Bmi-1 expression and the telomerase activity in human ovarian carcinoma OVCAR-3 cells following treatment with PTP at three concentrations of 0.5, 1, and 2 mg/mL for 48 h. MTT and colony-forming assays revealed that PTP had a significant inhibitory effect on the cell growth and colony formation of OVCAR-3 cells. Furthermore, Western blot and real-time PCR analysis showed that PTP inhibited Bmi-1 both in protein and transcript levels. Besides, the telomerase activity in OVCAR-3 cells was also downregulated after PTP treatment for 48 h. Taken together, the inhibitory effect of PTP on the cell growth was at least in part mediated via the downregulation of Bmi-1 expression and the telomerase activity in OVCAR-3 cells, and PTP might be a new candidate for chemotherapeutic agent against human ovarian cancer.</description><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cancer Research</subject><subject>Cell Cycle - drug effects</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Cellular biology</subject><subject>Enzymes</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Ovarian cancer</subject><subject>Ovarian Neoplasms - drug therapy</subject><subject>Ovarian Neoplasms - genetics</subject><subject>Ovarian Neoplasms - pathology</subject><subject>Polycomb Repressive Complex 1 - biosynthesis</subject><subject>Polygala</subject><subject>Polygala - chemistry</subject><subject>Polysaccharides - administration & dosage</subject><subject>Polysaccharides - chemistry</subject><subject>Research Article</subject><subject>Telomerase</subject><subject>Telomerase - biosynthesis</subject><subject>Telomerase - genetics</subject><issn>1010-4283</issn><issn>1423-0380</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkcuKFTEURQtR7Id-gBMJOOkeRPOsJENtfEGDd9COw6lUcjtNVeqaVKn1C361uVQrIoijHM5Ze4fNbppnlLykhKhXhXKmFCZUYGYYweuD5pQKxjHhmjysM6EEC6b5SXNWyh0hVBrTPm5OmJR1Jua0-bGbhnUPA6DZpyWGaYiADnVXwLlbyLH36GJ3s7tEMd3GLs4FOT8M6JArGXyGOU4JdSvK_pB9KTHt0ZsxYor8921Rz5B61E_fUvb7ZaiKysx-mMYqLx6Bm-PXOK9PmkcBhuKf3r_nzed3b2-uPuDrT-8_Xr2-xk5wPeMWTMvASeN6MEIGQRQPJGgJ0nFqOk6F6sCFllKtAJTsoNWBGw8kBAnAz5uLzbdm-LL4MtsxlmMoSH5aiqVaUyWM1ur_aKsEI0QoXtEXf6F305JTDXKkOKdcMlkpulEuT6VkH-whxxHyaimxx07t1qmtndpjp3atmuf3zks3-v634leJFWAbUOop7X3-4-t_uv4Ee6evJQ</recordid><startdate>20150401</startdate><enddate>20150401</enddate><creator>Zhang, Fubin</creator><creator>Song, Xiaowei</creator><creator>Li, Li</creator><creator>Wang, Jingfang</creator><creator>Lin, Leyuan</creator><creator>Li, Cong</creator><creator>Li, Hongtao</creator><creator>Lv, Yanju</creator><creator>Jin, Yinghua</creator><creator>Liu, Ying</creator><creator>Hu, Yu</creator><creator>Xin, Tao</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20150401</creationdate><title>Polygala tenuifolia polysaccharide (PTP) inhibits cell proliferation by repressing Bmi-1 expression and downregulating telomerase activity</title><author>Zhang, Fubin ; Song, Xiaowei ; Li, Li ; Wang, Jingfang ; Lin, Leyuan ; Li, Cong ; Li, Hongtao ; Lv, Yanju ; Jin, Yinghua ; Liu, Ying ; Hu, Yu ; Xin, Tao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-6a962ac59cda945f4073f0f85a5c319b3147bacf61187aa75ba68f39ea0ff5aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cancer Research</topic><topic>Cell Cycle - drug effects</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>Cellular biology</topic><topic>Enzymes</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Ovarian cancer</topic><topic>Ovarian Neoplasms - drug therapy</topic><topic>Ovarian Neoplasms - genetics</topic><topic>Ovarian Neoplasms - pathology</topic><topic>Polycomb Repressive Complex 1 - biosynthesis</topic><topic>Polygala</topic><topic>Polygala - chemistry</topic><topic>Polysaccharides - administration & dosage</topic><topic>Polysaccharides - chemistry</topic><topic>Research Article</topic><topic>Telomerase</topic><topic>Telomerase - biosynthesis</topic><topic>Telomerase - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Fubin</creatorcontrib><creatorcontrib>Song, Xiaowei</creatorcontrib><creatorcontrib>Li, Li</creatorcontrib><creatorcontrib>Wang, Jingfang</creatorcontrib><creatorcontrib>Lin, Leyuan</creatorcontrib><creatorcontrib>Li, Cong</creatorcontrib><creatorcontrib>Li, Hongtao</creatorcontrib><creatorcontrib>Lv, Yanju</creatorcontrib><creatorcontrib>Jin, Yinghua</creatorcontrib><creatorcontrib>Liu, Ying</creatorcontrib><creatorcontrib>Hu, Yu</creatorcontrib><creatorcontrib>Xin, Tao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Tumor biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Fubin</au><au>Song, Xiaowei</au><au>Li, Li</au><au>Wang, Jingfang</au><au>Lin, Leyuan</au><au>Li, Cong</au><au>Li, Hongtao</au><au>Lv, Yanju</au><au>Jin, Yinghua</au><au>Liu, Ying</au><au>Hu, Yu</au><au>Xin, Tao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polygala tenuifolia polysaccharide (PTP) inhibits cell proliferation by repressing Bmi-1 expression and downregulating telomerase activity</atitle><jtitle>Tumor biology</jtitle><stitle>Tumor Biol</stitle><addtitle>Tumour Biol</addtitle><date>2015-04-01</date><risdate>2015</risdate><volume>36</volume><issue>4</issue><spage>2907</spage><epage>2912</epage><pages>2907-2912</pages><issn>1010-4283</issn><eissn>1423-0380</eissn><abstract>In our previous study, we isolated a homogeneous polysaccharide (PTP) with antitumor activity from the roots of
Polygala tenuifolia
. In view of the close correlation between Bmi-1 expression and progression of ovarian cancer, we intend to elucidate the mechanism of its activity by determining the Bmi-1 expression and the telomerase activity in human ovarian carcinoma OVCAR-3 cells following treatment with PTP at three concentrations of 0.5, 1, and 2 mg/mL for 48 h. MTT and colony-forming assays revealed that PTP had a significant inhibitory effect on the cell growth and colony formation of OVCAR-3 cells. Furthermore, Western blot and real-time PCR analysis showed that PTP inhibited Bmi-1 both in protein and transcript levels. Besides, the telomerase activity in OVCAR-3 cells was also downregulated after PTP treatment for 48 h. Taken together, the inhibitory effect of PTP on the cell growth was at least in part mediated via the downregulation of Bmi-1 expression and the telomerase activity in OVCAR-3 cells, and PTP might be a new candidate for chemotherapeutic agent against human ovarian cancer.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>25501509</pmid><doi>10.1007/s13277-014-2920-y</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biomedical and Life Sciences Biomedicine Cancer Research Cell Cycle - drug effects Cell Line, Tumor Cell Proliferation - drug effects Cellular biology Enzymes Female Gene Expression Regulation, Neoplastic Humans Ovarian cancer Ovarian Neoplasms - drug therapy Ovarian Neoplasms - genetics Ovarian Neoplasms - pathology Polycomb Repressive Complex 1 - biosynthesis Polygala Polygala - chemistry Polysaccharides - administration & dosage Polysaccharides - chemistry Research Article Telomerase Telomerase - biosynthesis Telomerase - genetics |
| title | Polygala tenuifolia polysaccharide (PTP) inhibits cell proliferation by repressing Bmi-1 expression and downregulating telomerase activity |
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