A column switching ultrahigh-performance liquid chromatography-tandem mass spectrometry method to determine anandamide and 2-arachidonoylglycerol in plasma samples
This study reports a fast, sensitive, and selective column switching ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to determine the endocannabinoids (eCBs), anandamide (AEA), and 2-arachidonoylglycerol (2-AG) in plasma samples. This bidimensional system us...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2017-05, Vol.409 (14), p.3587-3596 |
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| creator | Marchioni, Camila de Souza, Israel Donizeti Grecco, Caroline Fernandes Crippa, José Alexandre Tumas, Vitor Queiroz, Maria Eugênia Costa |
| description | This study reports a fast, sensitive, and selective column switching ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to determine the endocannabinoids (eCBs), anandamide (AEA), and 2-arachidonoylglycerol (2-AG) in plasma samples. This bidimensional system used a restricted access media column (RP-8 ADS, 25 mm × 4 mm × 25 μM) in the first dimension and a core-shell Kinetex C18 (100 mm × 2, 1.7 mm × 1 μM) column in the second dimension, followed by detection in a mass spectrometer triple quadrupole (multiple reactions monitoring mode) operating in the positive mode. RP-8 ADS was used for trace enrichment of eCBs (reverse phase partitioning) and macromolecular matrix size exclusion; the core-shell column was used for the chromatographic separation. The column switching UHPLC-MS/MS method presented a linear range spanning from 0.1 ng mL
−1
(LOQ) to 6 ng mL
−1
for AEA and from 0.04 ng mL
−1
(LOQ) to 10 ng mL
−1
for 2-AG. Excluding the LLOQ values, the precision assays provided coefficients of variation lower than 8% and accuracy with relative standard error values lower than 14%. Neither carryover nor matrix effects were detected. This high-throughput column switching method compared to conventional methods is time saving as it involves fewer steps, consumes less solvent, and presents lower LLOQ. The column switching UHPLC-MS/MS method was successfully applied to determine AEA and 2-AG in plasma samples obtained from Alzheimer’s disease patients.
Graphical abstract
A column switching ultra high-performance liquid chromatography-tandem mass spectrometry method using RP-8 ADS column and core shell column to determine endocannabinoids in plasma samples |
| doi_str_mv | 10.1007/s00216-017-0300-3 |
| format | Article |
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−1
(LOQ) to 6 ng mL
−1
for AEA and from 0.04 ng mL
−1
(LOQ) to 10 ng mL
−1
for 2-AG. Excluding the LLOQ values, the precision assays provided coefficients of variation lower than 8% and accuracy with relative standard error values lower than 14%. Neither carryover nor matrix effects were detected. This high-throughput column switching method compared to conventional methods is time saving as it involves fewer steps, consumes less solvent, and presents lower LLOQ. The column switching UHPLC-MS/MS method was successfully applied to determine AEA and 2-AG in plasma samples obtained from Alzheimer’s disease patients.
Graphical abstract
A column switching ultra high-performance liquid chromatography-tandem mass spectrometry method using RP-8 ADS column and core shell column to determine endocannabinoids in plasma samples</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-017-0300-3</identifier><identifier>PMID: 28337517</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>2-Arachidonoylglycerol ; Alzheimer Disease - blood ; Analytical Chemistry ; Anandamide ; Arachidonic Acids - blood ; Biochemistry ; Cannabinoids ; Characterization and Evaluation of Materials ; Chemical properties ; Chemistry ; Chemistry and Materials Science ; Chromatography ; Chromatography, High Pressure Liquid - instrumentation ; Chromatography, High Pressure Liquid - methods ; Coefficient of variation ; Endocannabinoids - blood ; Equipment Design ; Error detection ; Food Science ; Glycerides - blood ; Humans ; Laboratory Medicine ; Limit of Detection ; Liquid chromatography ; Macromolecules ; Mass spectrometry ; Mass spectroscopy ; Matrices (mathematics) ; Monitoring/Environmental Analysis ; Polyunsaturated Alkamides - blood ; Quadrupoles ; Reproducibility of Results ; Research Paper ; Scientific imaging ; Spectroscopy ; Standard error ; Switching ; Tandem Mass Spectrometry - instrumentation ; Tandem Mass Spectrometry - methods</subject><ispartof>Analytical and bioanalytical chemistry, 2017-05, Vol.409 (14), p.3587-3596</ispartof><rights>Springer-Verlag Berlin Heidelberg 2017</rights><rights>COPYRIGHT 2017 Springer</rights><rights>Analytical and Bioanalytical Chemistry is a copyright of Springer, (2017). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-3b122967e96cb3266cffc52a4fa14e5c2f12f618012784cdf884a1b9df8da6d23</citedby><cites>FETCH-LOGICAL-c448t-3b122967e96cb3266cffc52a4fa14e5c2f12f618012784cdf884a1b9df8da6d23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-017-0300-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-017-0300-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28337517$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marchioni, Camila</creatorcontrib><creatorcontrib>de Souza, Israel Donizeti</creatorcontrib><creatorcontrib>Grecco, Caroline Fernandes</creatorcontrib><creatorcontrib>Crippa, José Alexandre</creatorcontrib><creatorcontrib>Tumas, Vitor</creatorcontrib><creatorcontrib>Queiroz, Maria Eugênia Costa</creatorcontrib><title>A column switching ultrahigh-performance liquid chromatography-tandem mass spectrometry method to determine anandamide and 2-arachidonoylglycerol in plasma samples</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>This study reports a fast, sensitive, and selective column switching ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to determine the endocannabinoids (eCBs), anandamide (AEA), and 2-arachidonoylglycerol (2-AG) in plasma samples. This bidimensional system used a restricted access media column (RP-8 ADS, 25 mm × 4 mm × 25 μM) in the first dimension and a core-shell Kinetex C18 (100 mm × 2, 1.7 mm × 1 μM) column in the second dimension, followed by detection in a mass spectrometer triple quadrupole (multiple reactions monitoring mode) operating in the positive mode. RP-8 ADS was used for trace enrichment of eCBs (reverse phase partitioning) and macromolecular matrix size exclusion; the core-shell column was used for the chromatographic separation. The column switching UHPLC-MS/MS method presented a linear range spanning from 0.1 ng mL
−1
(LOQ) to 6 ng mL
−1
for AEA and from 0.04 ng mL
−1
(LOQ) to 10 ng mL
−1
for 2-AG. Excluding the LLOQ values, the precision assays provided coefficients of variation lower than 8% and accuracy with relative standard error values lower than 14%. Neither carryover nor matrix effects were detected. This high-throughput column switching method compared to conventional methods is time saving as it involves fewer steps, consumes less solvent, and presents lower LLOQ. The column switching UHPLC-MS/MS method was successfully applied to determine AEA and 2-AG in plasma samples obtained from Alzheimer’s disease patients.
Graphical abstract
A column switching ultra high-performance liquid chromatography-tandem mass spectrometry method using RP-8 ADS column and core shell column to determine endocannabinoids in plasma samples</description><subject>2-Arachidonoylglycerol</subject><subject>Alzheimer Disease - blood</subject><subject>Analytical Chemistry</subject><subject>Anandamide</subject><subject>Arachidonic Acids - blood</subject><subject>Biochemistry</subject><subject>Cannabinoids</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemical properties</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid - instrumentation</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Coefficient of variation</subject><subject>Endocannabinoids - blood</subject><subject>Equipment Design</subject><subject>Error detection</subject><subject>Food Science</subject><subject>Glycerides - blood</subject><subject>Humans</subject><subject>Laboratory Medicine</subject><subject>Limit of Detection</subject><subject>Liquid chromatography</subject><subject>Macromolecules</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Matrices (mathematics)</subject><subject>Monitoring/Environmental Analysis</subject><subject>Polyunsaturated Alkamides - blood</subject><subject>Quadrupoles</subject><subject>Reproducibility of Results</subject><subject>Research Paper</subject><subject>Scientific imaging</subject><subject>Spectroscopy</subject><subject>Standard error</subject><subject>Switching</subject><subject>Tandem Mass Spectrometry - instrumentation</subject><subject>Tandem Mass Spectrometry - methods</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kcuKFTEQhhtRnHH0AdxIwI2bHlPp61kehvECA250Heokle4MufQk3ch5Hl_UHM44iCCBSpH6_kpRf1W9BX4NnA8fM-cC-prDUPOG87p5Vl1CD2Mt-o4_f8pbcVG9yvmec-hG6F9WF2JsmqGD4bL6tWcqus0Hln_aVc02TGxza8LZTnO9UDIxeQyKmLMPm9VMzSl6XOOUcJmP9YpBk2cec2Z5IbWWKq3pyEqco2ZrZJpWSt4GYhgKjd7qU6qZqDFh-VLHEI9uckdFKTpmA1scZo8so18c5dfVC4Mu05vH-6r68en2-82X-u7b5683-7tate241s0BhNj1A-16dWhE3ytjVCewNQgtdUoYEKZshIMYxlZpM44twmFXEo29Fs1V9eHcd0nxYaO8Sm-zIucwUNyyhHEE0Q9txwv6_h_0Pm4plOkk7AYQ4ykU6vpMTehI2mBiWawqp6zMqhjI2PK-7zq-64CLkwDOApVizomMXJL1mI4SuDxZLs-Wy2K5PFkum6J59zjKdvCknxR_PC6AOAO5lMJE6a9Z_9v1N-bOujA</recordid><startdate>20170501</startdate><enddate>20170501</enddate><creator>Marchioni, Camila</creator><creator>de Souza, Israel Donizeti</creator><creator>Grecco, Caroline Fernandes</creator><creator>Crippa, José Alexandre</creator><creator>Tumas, Vitor</creator><creator>Queiroz, Maria Eugênia Costa</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>H8G</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KB.</scope><scope>KR7</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20170501</creationdate><title>A column switching ultrahigh-performance liquid chromatography-tandem mass spectrometry method to determine anandamide and 2-arachidonoylglycerol in plasma samples</title><author>Marchioni, Camila ; de Souza, Israel Donizeti ; Grecco, Caroline Fernandes ; Crippa, José Alexandre ; Tumas, Vitor ; Queiroz, Maria Eugênia Costa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-3b122967e96cb3266cffc52a4fa14e5c2f12f618012784cdf884a1b9df8da6d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>2-Arachidonoylglycerol</topic><topic>Alzheimer Disease - blood</topic><topic>Analytical Chemistry</topic><topic>Anandamide</topic><topic>Arachidonic Acids - blood</topic><topic>Biochemistry</topic><topic>Cannabinoids</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemical properties</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid - instrumentation</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Coefficient of variation</topic><topic>Endocannabinoids - blood</topic><topic>Equipment Design</topic><topic>Error detection</topic><topic>Food Science</topic><topic>Glycerides - blood</topic><topic>Humans</topic><topic>Laboratory Medicine</topic><topic>Limit of Detection</topic><topic>Liquid chromatography</topic><topic>Macromolecules</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Matrices (mathematics)</topic><topic>Monitoring/Environmental Analysis</topic><topic>Polyunsaturated Alkamides - blood</topic><topic>Quadrupoles</topic><topic>Reproducibility of Results</topic><topic>Research Paper</topic><topic>Scientific imaging</topic><topic>Spectroscopy</topic><topic>Standard error</topic><topic>Switching</topic><topic>Tandem Mass Spectrometry - instrumentation</topic><topic>Tandem Mass Spectrometry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marchioni, Camila</creatorcontrib><creatorcontrib>de Souza, Israel Donizeti</creatorcontrib><creatorcontrib>Grecco, Caroline Fernandes</creatorcontrib><creatorcontrib>Crippa, José Alexandre</creatorcontrib><creatorcontrib>Tumas, Vitor</creatorcontrib><creatorcontrib>Queiroz, Maria Eugênia Costa</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marchioni, Camila</au><au>de Souza, Israel Donizeti</au><au>Grecco, Caroline Fernandes</au><au>Crippa, José Alexandre</au><au>Tumas, Vitor</au><au>Queiroz, Maria Eugênia Costa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A column switching ultrahigh-performance liquid chromatography-tandem mass spectrometry method to determine anandamide and 2-arachidonoylglycerol in plasma samples</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2017-05-01</date><risdate>2017</risdate><volume>409</volume><issue>14</issue><spage>3587</spage><epage>3596</epage><pages>3587-3596</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>This study reports a fast, sensitive, and selective column switching ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to determine the endocannabinoids (eCBs), anandamide (AEA), and 2-arachidonoylglycerol (2-AG) in plasma samples. This bidimensional system used a restricted access media column (RP-8 ADS, 25 mm × 4 mm × 25 μM) in the first dimension and a core-shell Kinetex C18 (100 mm × 2, 1.7 mm × 1 μM) column in the second dimension, followed by detection in a mass spectrometer triple quadrupole (multiple reactions monitoring mode) operating in the positive mode. RP-8 ADS was used for trace enrichment of eCBs (reverse phase partitioning) and macromolecular matrix size exclusion; the core-shell column was used for the chromatographic separation. The column switching UHPLC-MS/MS method presented a linear range spanning from 0.1 ng mL
−1
(LOQ) to 6 ng mL
−1
for AEA and from 0.04 ng mL
−1
(LOQ) to 10 ng mL
−1
for 2-AG. Excluding the LLOQ values, the precision assays provided coefficients of variation lower than 8% and accuracy with relative standard error values lower than 14%. Neither carryover nor matrix effects were detected. This high-throughput column switching method compared to conventional methods is time saving as it involves fewer steps, consumes less solvent, and presents lower LLOQ. The column switching UHPLC-MS/MS method was successfully applied to determine AEA and 2-AG in plasma samples obtained from Alzheimer’s disease patients.
Graphical abstract
A column switching ultra high-performance liquid chromatography-tandem mass spectrometry method using RP-8 ADS column and core shell column to determine endocannabinoids in plasma samples</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>28337517</pmid><doi>10.1007/s00216-017-0300-3</doi><tpages>10</tpages></addata></record> |
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| issn | 1618-2642 1618-2650 |
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| source | MEDLINE; SpringerNature Journals |
| subjects | 2-Arachidonoylglycerol Alzheimer Disease - blood Analytical Chemistry Anandamide Arachidonic Acids - blood Biochemistry Cannabinoids Characterization and Evaluation of Materials Chemical properties Chemistry Chemistry and Materials Science Chromatography Chromatography, High Pressure Liquid - instrumentation Chromatography, High Pressure Liquid - methods Coefficient of variation Endocannabinoids - blood Equipment Design Error detection Food Science Glycerides - blood Humans Laboratory Medicine Limit of Detection Liquid chromatography Macromolecules Mass spectrometry Mass spectroscopy Matrices (mathematics) Monitoring/Environmental Analysis Polyunsaturated Alkamides - blood Quadrupoles Reproducibility of Results Research Paper Scientific imaging Spectroscopy Standard error Switching Tandem Mass Spectrometry - instrumentation Tandem Mass Spectrometry - methods |
| title | A column switching ultrahigh-performance liquid chromatography-tandem mass spectrometry method to determine anandamide and 2-arachidonoylglycerol in plasma samples |
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