A novel flavin-containing monooxygenase from Methylophaga sp. strain SK1 and its indigo synthesis in Escherichia coli

We cloned a gene from Methylophaga sp. strain SK1. This gene was responsible for producing, the blue pigment, indigo. The complete open reading frame was 1371 bp long, which encodes a protein of 456 amino acids. The molecular mass of the encoded protein was 105 kDa, consisting of homodimer of 54 kDa...

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Veröffentlicht in:	Biochemical and biophysical research communications 2003-07, Vol.306 (4), p.930-936
Hauptverfasser:	Choi, Hack Sun, Kim, Jin Kwon, Cho, Eun Hee, Kim, Yong Chul, Kim, Jae Il, Kim, Si Wouk
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Motifs Amino Acid Sequence Chromatography, High Pressure Liquid Cloning, Molecular Dimerization Escherichia coli - metabolism Flavin-Adenine Dinucleotide - metabolism Flavin-containing monooxygenase Flavins - chemistry Fungal Proteins - chemistry Gammaproteobacteria - enzymology Gene Library Humans Hydrogen-Ion Concentration Isoelectric Focusing Kinetics Methylophaga sp. strain SK1 Microbial indigo synthesis Mixed Function Oxygenases - chemistry Molecular Sequence Data Mutagenesis, Site-Directed NADP - metabolism Open Reading Frames Pigments, Biological - biosynthesis Plant Proteins - chemistry Plasmids - metabolism Protein Structure, Tertiary Sequence Homology, Amino Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry Substrate Specificity Time Factors
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