Interlaboratory Reproducibility of a Targeted Metabolomics Platform for Analysis of Human Serum and Plasma

Interlaboratory Reproducibility of a Targeted Metabolomics Platform for Analysis of Human Serum and Plasma

A critical question facing the field of metabolomics is whether data obtained from different centers can be effectively compared and combined. An important aspect of this is the interlaboratory precision (reproducibility) of the analytical protocols used. We analyzed human samples in six laboratorie...

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Veröffentlicht in:Analytical chemistry (Washington) 2017-01, Vol.89 (1), p.656-665
Hauptverfasser: Siskos, Alexandros P, Jain, Pooja, Römisch-Margl, Werner, Bennett, Mark, Achaintre, David, Asad, Yasmin, Marney, Luke, Richardson, Larissa, Koulman, Albert, Griffin, Julian L, Raynaud, Florence, Scalbert, Augustin, Adamski, Jerzy, Prehn, Cornelia, Keun, Hector C
Format: Artikel
Sprache:eng
Schlagworte:
Analytical chemistry
Chromatography
Flow injection analysis
Humans
Interlaboratory
Laboratories
Limit of Detection
Liquid chromatography
Mass spectrometry
Metabolites
Metabolomics - methods
Metabolomics - standards
Plasma
Proteins
Quality Control
Reference Standards
Reproducibility
Reproducibility of Results
Serums
Tandem Mass Spectrometry
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Zusammenfassung:A critical question facing the field of metabolomics is whether data obtained from different centers can be effectively compared and combined. An important aspect of this is the interlaboratory precision (reproducibility) of the analytical protocols used. We analyzed human samples in six laboratories using different instrumentation but a common protocol (the AbsoluteIDQ p180 kit) for the measurement of 189 metabolites via liquid chromatography (LC) or flow injection analysis (FIA) coupled to tandem mass spectrometry (MS/MS). In spiked quality control (QC) samples 82% of metabolite measurements had an interlaboratory precision of
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.6b02930