Investigation of cell adhesion in chitosan membranes for peripheral nerve regeneration
Peripheral nerve injuries have produced major concerns in regenerative medicine for several years, as the recovery of normal nerve function continues to be a significant clinical challenge. Chitosan (CHT), because of its good biocompatibility, biodegradability and physicochemical properties, has bee...
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| Veröffentlicht in: | Materials Science & Engineering C 2017-02, Vol.71, p.1122-1134 |
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| creator | Carvalho, Cristiana R. López-Cebral, Rita Silva-Correia, Joana Silva, Joana M. Mano, João F. Silva, Tiago H. Freier, Thomas Reis, Rui L. Oliveira, Joaquim M. |
| description | Peripheral nerve injuries have produced major concerns in regenerative medicine for several years, as the recovery of normal nerve function continues to be a significant clinical challenge. Chitosan (CHT), because of its good biocompatibility, biodegradability and physicochemical properties, has been widely used as a biomaterial in tissue engineering scaffolding. In this study, CHT membranes were produced with three different Degrees of Acetylation (DA), envisioning its application in peripheral nerve regeneration. The three CHT membranes (DA I: 1%, DA II: 2%, DA III: 5%) were extensively characterized and were found to have a smooth and flat surface, with DA III membrane having slightly higher roughness and surface energy. All the membranes presented suitable mechanical properties and did not show any signs of calcification after SBF test. Biodegradability was similar for all samples, and adequate to physically support neurite outgrowth. The in vitro cell culture results indicate selective cell adhesion. The CHT membranes favoured Schwann cells invasion and proliferation, with a display of appropriate cytoskeletal morphology. At the same time they presented low fibroblast infiltration. This fact may be greatly beneficial for the prevention of fibrotic tissue formation, a common phenomenon impairing peripheral nerve regeneration. The great deal of results obtained during this work permitted to select the formulation with the greatest potential for further biological tests.
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•Three chitosan membranes were produced with very specific degrees of acetylation (DA I: 1%, DA II: 2%, DA III: 5%).•Physicochemical characterization of the membranes showed their suitability for peripheral nerve regeneration purposes.•In vitro cellular tests confirmed the potential of the membranes as peripheral nerve regeneration systems.•The results indicated that DA III membrane should be the one considered for further peripheral nerve regeneration studies. |
| doi_str_mv | 10.1016/j.msec.2016.11.100 |
| format | Article |
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[Display omitted]
•Three chitosan membranes were produced with very specific degrees of acetylation (DA I: 1%, DA II: 2%, DA III: 5%).•Physicochemical characterization of the membranes showed their suitability for peripheral nerve regeneration purposes.•In vitro cellular tests confirmed the potential of the membranes as peripheral nerve regeneration systems.•The results indicated that DA III membrane should be the one considered for further peripheral nerve regeneration studies.</description><identifier>ISSN: 0928-4931</identifier><identifier>EISSN: 1873-0191</identifier><identifier>DOI: 10.1016/j.msec.2016.11.100</identifier><identifier>PMID: 27987669</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Acetylation ; Animals ; Axonogenesis ; Biocompatibility ; Biodegradability ; Biodegradation ; Biomaterials ; Biomedical materials ; Calcification ; Cell Adhesion ; Cell adhesion & migration ; Cell culture ; Cell Line, Transformed ; Cell Proliferation ; Chitosan ; Chitosan - chemistry ; Cytoskeleton ; Degree of acetylation ; Fibrosis ; Infiltration ; L929 mouse fibroblast cells ; Materials science ; Mechanical properties ; Membranes ; Membranes, Artificial ; Mice ; Nerve Regeneration ; Peripheral nerve injuries ; Peripheral nerves ; Peripheral Nerves - cytology ; Peripheral Nerves - metabolism ; Physicochemical properties ; Regeneration ; Regeneration (physiology) ; Regenerative medicine ; Roughness ; Scaffolding ; Schwann cells ; Schwann Cells - cytology ; Schwann Cells - metabolism ; Surface energy ; Surface properties ; Tissue engineering</subject><ispartof>Materials Science & Engineering C, 2017-02, Vol.71, p.1122-1134</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><rights>Copyright Elsevier BV Feb 1, 2017</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c556t-bb4140937c1efad5209198fca1f46cd9691a75e30651746706a585e89f84ffb23</citedby><cites>FETCH-LOGICAL-c556t-bb4140937c1efad5209198fca1f46cd9691a75e30651746706a585e89f84ffb23</cites><orcidid>0000-0001-7052-8837</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.msec.2016.11.100$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27987669$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carvalho, Cristiana R.</creatorcontrib><creatorcontrib>López-Cebral, Rita</creatorcontrib><creatorcontrib>Silva-Correia, Joana</creatorcontrib><creatorcontrib>Silva, Joana M.</creatorcontrib><creatorcontrib>Mano, João F.</creatorcontrib><creatorcontrib>Silva, Tiago H.</creatorcontrib><creatorcontrib>Freier, Thomas</creatorcontrib><creatorcontrib>Reis, Rui L.</creatorcontrib><creatorcontrib>Oliveira, Joaquim M.</creatorcontrib><title>Investigation of cell adhesion in chitosan membranes for peripheral nerve regeneration</title><title>Materials Science & Engineering C</title><addtitle>Mater Sci Eng C Mater Biol Appl</addtitle><description>Peripheral nerve injuries have produced major concerns in regenerative medicine for several years, as the recovery of normal nerve function continues to be a significant clinical challenge. Chitosan (CHT), because of its good biocompatibility, biodegradability and physicochemical properties, has been widely used as a biomaterial in tissue engineering scaffolding. In this study, CHT membranes were produced with three different Degrees of Acetylation (DA), envisioning its application in peripheral nerve regeneration. The three CHT membranes (DA I: 1%, DA II: 2%, DA III: 5%) were extensively characterized and were found to have a smooth and flat surface, with DA III membrane having slightly higher roughness and surface energy. All the membranes presented suitable mechanical properties and did not show any signs of calcification after SBF test. Biodegradability was similar for all samples, and adequate to physically support neurite outgrowth. The in vitro cell culture results indicate selective cell adhesion. The CHT membranes favoured Schwann cells invasion and proliferation, with a display of appropriate cytoskeletal morphology. At the same time they presented low fibroblast infiltration. This fact may be greatly beneficial for the prevention of fibrotic tissue formation, a common phenomenon impairing peripheral nerve regeneration. The great deal of results obtained during this work permitted to select the formulation with the greatest potential for further biological tests.
[Display omitted]
•Three chitosan membranes were produced with very specific degrees of acetylation (DA I: 1%, DA II: 2%, DA III: 5%).•Physicochemical characterization of the membranes showed their suitability for peripheral nerve regeneration purposes.•In vitro cellular tests confirmed the potential of the membranes as peripheral nerve regeneration systems.•The results indicated that DA III membrane should be the one considered for further peripheral nerve regeneration studies.</description><subject>Acetylation</subject><subject>Animals</subject><subject>Axonogenesis</subject><subject>Biocompatibility</subject><subject>Biodegradability</subject><subject>Biodegradation</subject><subject>Biomaterials</subject><subject>Biomedical materials</subject><subject>Calcification</subject><subject>Cell Adhesion</subject><subject>Cell adhesion & migration</subject><subject>Cell culture</subject><subject>Cell Line, Transformed</subject><subject>Cell Proliferation</subject><subject>Chitosan</subject><subject>Chitosan - 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Academic</collection><collection>Neurosciences Abstracts</collection><jtitle>Materials Science & Engineering C</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carvalho, Cristiana R.</au><au>López-Cebral, Rita</au><au>Silva-Correia, Joana</au><au>Silva, Joana M.</au><au>Mano, João F.</au><au>Silva, Tiago H.</au><au>Freier, Thomas</au><au>Reis, Rui L.</au><au>Oliveira, Joaquim M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigation of cell adhesion in chitosan membranes for peripheral nerve regeneration</atitle><jtitle>Materials Science & Engineering C</jtitle><addtitle>Mater Sci Eng C Mater Biol Appl</addtitle><date>2017-02-01</date><risdate>2017</risdate><volume>71</volume><spage>1122</spage><epage>1134</epage><pages>1122-1134</pages><issn>0928-4931</issn><eissn>1873-0191</eissn><abstract>Peripheral nerve injuries have produced major concerns in regenerative medicine for several years, as the recovery of normal nerve function continues to be a significant clinical challenge. Chitosan (CHT), because of its good biocompatibility, biodegradability and physicochemical properties, has been widely used as a biomaterial in tissue engineering scaffolding. In this study, CHT membranes were produced with three different Degrees of Acetylation (DA), envisioning its application in peripheral nerve regeneration. The three CHT membranes (DA I: 1%, DA II: 2%, DA III: 5%) were extensively characterized and were found to have a smooth and flat surface, with DA III membrane having slightly higher roughness and surface energy. All the membranes presented suitable mechanical properties and did not show any signs of calcification after SBF test. Biodegradability was similar for all samples, and adequate to physically support neurite outgrowth. The in vitro cell culture results indicate selective cell adhesion. The CHT membranes favoured Schwann cells invasion and proliferation, with a display of appropriate cytoskeletal morphology. At the same time they presented low fibroblast infiltration. This fact may be greatly beneficial for the prevention of fibrotic tissue formation, a common phenomenon impairing peripheral nerve regeneration. The great deal of results obtained during this work permitted to select the formulation with the greatest potential for further biological tests.
[Display omitted]
•Three chitosan membranes were produced with very specific degrees of acetylation (DA I: 1%, DA II: 2%, DA III: 5%).•Physicochemical characterization of the membranes showed their suitability for peripheral nerve regeneration purposes.•In vitro cellular tests confirmed the potential of the membranes as peripheral nerve regeneration systems.•The results indicated that DA III membrane should be the one considered for further peripheral nerve regeneration studies.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>27987669</pmid><doi>10.1016/j.msec.2016.11.100</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-7052-8837</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acetylation Animals Axonogenesis Biocompatibility Biodegradability Biodegradation Biomaterials Biomedical materials Calcification Cell Adhesion Cell adhesion & migration Cell culture Cell Line, Transformed Cell Proliferation Chitosan Chitosan - chemistry Cytoskeleton Degree of acetylation Fibrosis Infiltration L929 mouse fibroblast cells Materials science Mechanical properties Membranes Membranes, Artificial Mice Nerve Regeneration Peripheral nerve injuries Peripheral nerves Peripheral Nerves - cytology Peripheral Nerves - metabolism Physicochemical properties Regeneration Regeneration (physiology) Regenerative medicine Roughness Scaffolding Schwann cells Schwann Cells - cytology Schwann Cells - metabolism Surface energy Surface properties Tissue engineering |
| title | Investigation of cell adhesion in chitosan membranes for peripheral nerve regeneration |
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