Investigating cell‐substrate and cell–cell interactions by means of single‐cell‐probe force spectroscopy
ABSTRACT Cell adhesion forces are typically a mixture of specific and nonspecific cell‐substrate and cell–cell interactions. In order to resolve these phenomena, Atomic Force Microscopy appears as a powerful device which can measure cell parameters by means of manipulation of single cells. This meth...
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| Veröffentlicht in: | Microscopy research and technique 2017-01, Vol.80 (1), p.124-130 |
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| creator | Moreno‐Cencerrado, Alberto Iturri, Jagoba Pecorari, Ilaria D.M. Vivanco, Maria Sbaizero, Orfeo Toca‐Herrera, José L. |
| description | ABSTRACT
Cell adhesion forces are typically a mixture of specific and nonspecific cell‐substrate and cell–cell interactions. In order to resolve these phenomena, Atomic Force Microscopy appears as a powerful device which can measure cell parameters by means of manipulation of single cells. This method, commonly known as cell‐probe force spectroscopy, allows us to control the force applied, the area of interest, the approach/retracting speed, the force rate, and the time of interaction. Here, we developed a novel approach for in situ cantilever cell capturing and measurement of specific cell interactions. In particular, we present a new setup consisting of two different half‐surfaces coated either with recrystallized SbpA bacterial cell surface layer proteins (S‐layers) or integrin binding Fibronectin, on which MCF‐7 breast cancer cells are incubated. The presence of a clear physical boundary between both surfaces benefits for a quick detection of the region under analysis. Thus, quantitative results about SbpA‐cell and Fibronectin‐cell adhesion forces as a function of the contact time are described. Additionally, the importance of the cell spreading in cell–cell interactions has been studied for surfaces coated with two different Fibronectin concentrations: 20 μg/mL (FN20) and 100 μg/mL (FN100), which impact the number of substrate receptors. Microsc. Res. Tech. 80:124–130, 2017. © 2016 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/jemt.22706 |
| format | Article |
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Cell adhesion forces are typically a mixture of specific and nonspecific cell‐substrate and cell–cell interactions. In order to resolve these phenomena, Atomic Force Microscopy appears as a powerful device which can measure cell parameters by means of manipulation of single cells. This method, commonly known as cell‐probe force spectroscopy, allows us to control the force applied, the area of interest, the approach/retracting speed, the force rate, and the time of interaction. Here, we developed a novel approach for in situ cantilever cell capturing and measurement of specific cell interactions. In particular, we present a new setup consisting of two different half‐surfaces coated either with recrystallized SbpA bacterial cell surface layer proteins (S‐layers) or integrin binding Fibronectin, on which MCF‐7 breast cancer cells are incubated. The presence of a clear physical boundary between both surfaces benefits for a quick detection of the region under analysis. Thus, quantitative results about SbpA‐cell and Fibronectin‐cell adhesion forces as a function of the contact time are described. Additionally, the importance of the cell spreading in cell–cell interactions has been studied for surfaces coated with two different Fibronectin concentrations: 20 μg/mL (FN20) and 100 μg/mL (FN100), which impact the number of substrate receptors. Microsc. Res. Tech. 80:124–130, 2017. © 2016 Wiley Periodicals, Inc.</description><identifier>ISSN: 1059-910X</identifier><identifier>EISSN: 1097-0029</identifier><identifier>DOI: 10.1002/jemt.22706</identifier><identifier>PMID: 27341785</identifier><identifier>CODEN: MRTEEO</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>atomic force microscopy ; Bacteria ; Bacterial Proteins - chemistry ; Binding ; Breast ; Cell Adhesion ; Cell Communication ; Coating ; Devices ; Fibronectin ; Fibronectins - chemistry ; force spectroscopy ; Humans ; MCF-7 Cells ; Microscopy, Atomic Force ; Monosaccharide Transport Proteins - chemistry ; Protein Binding ; Recrystallization ; single‐cell probe ; Spectroscopy ; Surface Properties ; S‐layer proteins</subject><ispartof>Microscopy research and technique, 2017-01, Vol.80 (1), p.124-130</ispartof><rights>2016 Wiley Periodicals, Inc.</rights><rights>2017 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4566-30ffdbb7b124133527dca7c665a4eb53294ac0f8187dd602816b3d22b254b6d23</citedby><cites>FETCH-LOGICAL-c4566-30ffdbb7b124133527dca7c665a4eb53294ac0f8187dd602816b3d22b254b6d23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjemt.22706$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjemt.22706$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27341785$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moreno‐Cencerrado, Alberto</creatorcontrib><creatorcontrib>Iturri, Jagoba</creatorcontrib><creatorcontrib>Pecorari, Ilaria</creatorcontrib><creatorcontrib>D.M. Vivanco, Maria</creatorcontrib><creatorcontrib>Sbaizero, Orfeo</creatorcontrib><creatorcontrib>Toca‐Herrera, José L.</creatorcontrib><title>Investigating cell‐substrate and cell–cell interactions by means of single‐cell‐probe force spectroscopy</title><title>Microscopy research and technique</title><addtitle>Microsc Res Tech</addtitle><description>ABSTRACT
Cell adhesion forces are typically a mixture of specific and nonspecific cell‐substrate and cell–cell interactions. In order to resolve these phenomena, Atomic Force Microscopy appears as a powerful device which can measure cell parameters by means of manipulation of single cells. This method, commonly known as cell‐probe force spectroscopy, allows us to control the force applied, the area of interest, the approach/retracting speed, the force rate, and the time of interaction. Here, we developed a novel approach for in situ cantilever cell capturing and measurement of specific cell interactions. In particular, we present a new setup consisting of two different half‐surfaces coated either with recrystallized SbpA bacterial cell surface layer proteins (S‐layers) or integrin binding Fibronectin, on which MCF‐7 breast cancer cells are incubated. The presence of a clear physical boundary between both surfaces benefits for a quick detection of the region under analysis. Thus, quantitative results about SbpA‐cell and Fibronectin‐cell adhesion forces as a function of the contact time are described. Additionally, the importance of the cell spreading in cell–cell interactions has been studied for surfaces coated with two different Fibronectin concentrations: 20 μg/mL (FN20) and 100 μg/mL (FN100), which impact the number of substrate receptors. Microsc. Res. Tech. 80:124–130, 2017. © 2016 Wiley Periodicals, Inc.</description><subject>atomic force microscopy</subject><subject>Bacteria</subject><subject>Bacterial Proteins - chemistry</subject><subject>Binding</subject><subject>Breast</subject><subject>Cell Adhesion</subject><subject>Cell Communication</subject><subject>Coating</subject><subject>Devices</subject><subject>Fibronectin</subject><subject>Fibronectins - chemistry</subject><subject>force spectroscopy</subject><subject>Humans</subject><subject>MCF-7 Cells</subject><subject>Microscopy, Atomic Force</subject><subject>Monosaccharide Transport Proteins - chemistry</subject><subject>Protein Binding</subject><subject>Recrystallization</subject><subject>single‐cell probe</subject><subject>Spectroscopy</subject><subject>Surface Properties</subject><subject>S‐layer proteins</subject><issn>1059-910X</issn><issn>1097-0029</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctKxDAUhoMoOl42PoAU3IjQ8SRpknYpw3hjxM0I7kqSptKhN5NWmZ2PIPiGPompM7pwIa7O4fCdn_OfH6FDDGMMQM4WpurGhAjgG2iEIRGhnyabQ8-SMMHwsIN2nVsAYMxwtI12iKARFjEbofa6fjauKx5lV9SPgTZl-fH65nrlOis7E8g6Ww_fhxIUdWes1F3R1C5Qy6Ay0jdNHji_Xhq_u5ZobaNMkDdWm8C1Rne2cbppl_toK5elMwfruofuL6bzyVU4u7u8npzPQh0xzkMKeZ4pJRQmEaaUEZFpKTTnTEZGMUqSSGrIYxyLLONAYswVzQhRhEWKZ4TuoZOVrj_kqfcW06pww22yNk3vUhzHAMD9Q_6BEu6fy0B49PgXumh6W3sjnmI0ooCBe-p0RWlv2lmTp60tKmmXKYZ0iCwdIku_IvPw0VqyV5XJftDvjDyAV8BLUZrlH1LpzfR2vhL9BNL_pXQ</recordid><startdate>201701</startdate><enddate>201701</enddate><creator>Moreno‐Cencerrado, Alberto</creator><creator>Iturri, Jagoba</creator><creator>Pecorari, Ilaria</creator><creator>D.M. 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Vivanco, Maria ; Sbaizero, Orfeo ; Toca‐Herrera, José L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4566-30ffdbb7b124133527dca7c665a4eb53294ac0f8187dd602816b3d22b254b6d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>atomic force microscopy</topic><topic>Bacteria</topic><topic>Bacterial Proteins - chemistry</topic><topic>Binding</topic><topic>Breast</topic><topic>Cell Adhesion</topic><topic>Cell Communication</topic><topic>Coating</topic><topic>Devices</topic><topic>Fibronectin</topic><topic>Fibronectins - chemistry</topic><topic>force spectroscopy</topic><topic>Humans</topic><topic>MCF-7 Cells</topic><topic>Microscopy, Atomic Force</topic><topic>Monosaccharide Transport Proteins - chemistry</topic><topic>Protein Binding</topic><topic>Recrystallization</topic><topic>single‐cell probe</topic><topic>Spectroscopy</topic><topic>Surface Properties</topic><topic>S‐layer proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moreno‐Cencerrado, Alberto</creatorcontrib><creatorcontrib>Iturri, Jagoba</creatorcontrib><creatorcontrib>Pecorari, Ilaria</creatorcontrib><creatorcontrib>D.M. 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Vivanco, Maria</au><au>Sbaizero, Orfeo</au><au>Toca‐Herrera, José L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigating cell‐substrate and cell–cell interactions by means of single‐cell‐probe force spectroscopy</atitle><jtitle>Microscopy research and technique</jtitle><addtitle>Microsc Res Tech</addtitle><date>2017-01</date><risdate>2017</risdate><volume>80</volume><issue>1</issue><spage>124</spage><epage>130</epage><pages>124-130</pages><issn>1059-910X</issn><eissn>1097-0029</eissn><coden>MRTEEO</coden><abstract>ABSTRACT
Cell adhesion forces are typically a mixture of specific and nonspecific cell‐substrate and cell–cell interactions. In order to resolve these phenomena, Atomic Force Microscopy appears as a powerful device which can measure cell parameters by means of manipulation of single cells. This method, commonly known as cell‐probe force spectroscopy, allows us to control the force applied, the area of interest, the approach/retracting speed, the force rate, and the time of interaction. Here, we developed a novel approach for in situ cantilever cell capturing and measurement of specific cell interactions. In particular, we present a new setup consisting of two different half‐surfaces coated either with recrystallized SbpA bacterial cell surface layer proteins (S‐layers) or integrin binding Fibronectin, on which MCF‐7 breast cancer cells are incubated. The presence of a clear physical boundary between both surfaces benefits for a quick detection of the region under analysis. Thus, quantitative results about SbpA‐cell and Fibronectin‐cell adhesion forces as a function of the contact time are described. Additionally, the importance of the cell spreading in cell–cell interactions has been studied for surfaces coated with two different Fibronectin concentrations: 20 μg/mL (FN20) and 100 μg/mL (FN100), which impact the number of substrate receptors. Microsc. Res. Tech. 80:124–130, 2017. © 2016 Wiley Periodicals, Inc.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>27341785</pmid><doi>10.1002/jemt.22706</doi><tpages>7</tpages></addata></record> |
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| ispartof | Microscopy research and technique, 2017-01, Vol.80 (1), p.124-130 |
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| subjects | atomic force microscopy Bacteria Bacterial Proteins - chemistry Binding Breast Cell Adhesion Cell Communication Coating Devices Fibronectin Fibronectins - chemistry force spectroscopy Humans MCF-7 Cells Microscopy, Atomic Force Monosaccharide Transport Proteins - chemistry Protein Binding Recrystallization single‐cell probe Spectroscopy Surface Properties S‐layer proteins |
| title | Investigating cell‐substrate and cell–cell interactions by means of single‐cell‐probe force spectroscopy |
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